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The map label for this gene is rfbB2

Identifier: 187730265

GI number: 187730265

Start: 1098421

End: 1099506

Strand: Direct

Name: rfbB2

Synonym: SbBS512_E1192

Alternate gene names: 187730265

Gene position: 1098421-1099506 (Clockwise)

Preceding gene: 187732350

Following gene: 187730759

Centisome position: 23.8

GC content: 43.09

Gene sequence:

>1086_bases
GTGAAGATACTTGTTACTGGTGGCGCAGGATTTATTGGTTCTGCTGTAGTTCGTCACATTATAAATAATAGGCAGGATAG
TGTTGTTAATGTCGATAAATTAACGTACGCCGGAAACCTGGAATCACTTGCTGATGTTTCTGATTCTGAACGCTATGTTT
TTGAACATGCGGATATTTGCGATGCTGCTGCAATGGCGCGGATTTTTGCTCAGCATCAGCCGGATGCAGTGATGCACCTG
GCTGCTGAAAGCCATGTTGACCGTTCAATTACAGGTCCTGCGGCATTTATTGAAACCAATATTGTTGGTACTTATGTCCT
TTTAGAAGCCGCTCGTAATTACTGGTCTGCTCTTGATAGCGACAAGAAAAATAGCTTCCGTTTTCATCATATTTCTACTG
ACGAAGTCTATGGTGATTTGCCTCATCCTGACGAGGTAAATATTAAAGAAGGATTACCCTTATTTACTGAGACAACAGCT
TACGCGCCAAGCAGCCCTTATTCCGCATCCAAAGCATCCAGCGATCATTTAGTCCGCGCGTGGAAACGTACCTATGGTTT
ACCGACCATTGTGACTAATTGCTCTAACAATTATGGTCCTTATCATTTCCCGGAAAAATTGATTCCATTGGTTATTCTCA
ATGCTCTGGAAGGTAAAGGATTACCTATTTATGGTAAAGGGGATCAAATTCGCGACTGGCTGTATGTTGAAGATCATGCG
CGTGCGTTATATACCGTCGTAACCGAAGGTAAAGCGGGTGAAACTTATAACATTGGTGGACACAACGAAAAGAAAAACAT
CGATGTAGTGCTCACTATTTGTGATTTGTTGGATGAGATTGTCCCGAAAGAGAAATCTTACCGCGAGCAAATTACTTATG
TTACCGATCGTCCGGGACACGATCGCCGTTATGCGATTGATGCTGAGAAGATTGGTCGCGAATTGGGATGGAAACCACAG
GAAACGTTTGAGAGTGGGATTCGTAAAACGGTGGAATGGTACCTGTCCAATACAAAATGGGTTGATAATGTGAAAAGTGG
TGCCTATCAATCGTGGATTGAACAGAACTATGAGGGCCGCCAGTAA

Upstream 100 bases:

>100_bases
GCTGTAAAGCCAGGGGCGGTAGCGTGCATTAATACCTCTATTAATCAAACTAAGAGCCGCTAATTTCACAGCATGCTCTG
AAGTAATATGGAATAAAAAA

Downstream 100 bases:

>100_bases
TGAATATCCTCCTTTTTGGCAAAACAGGGCAGGTAGGTTGGGAACTACAGCGTGCTCTGGCACCTCTGGGTAATTTGATT
GCTCTTGATGTTCACTCCAC

Product: dTDP-glucose 4,6 dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 361; Mature: 361

Protein sequence:

>361_residues
MKILVTGGAGFIGSAVVRHIINNRQDSVVNVDKLTYAGNLESLADVSDSERYVFEHADICDAAAMARIFAQHQPDAVMHL
AAESHVDRSITGPAAFIETNIVGTYVLLEAARNYWSALDSDKKNSFRFHHISTDEVYGDLPHPDEVNIKEGLPLFTETTA
YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEGKGLPIYGKGDQIRDWLYVEDHA
RALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDLLDEIVPKEKSYREQITYVTDRPGHDRRYAIDAEKIGRELGWKPQ
ETFESGIRKTVEWYLSNTKWVDNVKSGAYQSWIEQNYEGRQ

Sequences:

>Translated_361_residues
MKILVTGGAGFIGSAVVRHIINNRQDSVVNVDKLTYAGNLESLADVSDSERYVFEHADICDAAAMARIFAQHQPDAVMHL
AAESHVDRSITGPAAFIETNIVGTYVLLEAARNYWSALDSDKKNSFRFHHISTDEVYGDLPHPDEVNIKEGLPLFTETTA
YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEGKGLPIYGKGDQIRDWLYVEDHA
RALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDLLDEIVPKEKSYREQITYVTDRPGHDRRYAIDAEKIGRELGWKPQ
ETFESGIRKTVEWYLSNTKWVDNVKSGAYQSWIEQNYEGRQ
>Mature_361_residues
MKILVTGGAGFIGSAVVRHIINNRQDSVVNVDKLTYAGNLESLADVSDSERYVFEHADICDAAAMARIFAQHQPDAVMHL
AAESHVDRSITGPAAFIETNIVGTYVLLEAARNYWSALDSDKKNSFRFHHISTDEVYGDLPHPDEVNIKEGLPLFTETTA
YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEGKGLPIYGKGDQIRDWLYVEDHA
RALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDLLDEIVPKEKSYREQITYVTDRPGHDRRYAIDAEKIGRELGWKPQ
ETFESGIRKTVEWYLSNTKWVDNVKSGAYQSWIEQNYEGRQ

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=337, Percent_Identity=35.3115727002967, Blast_Score=219, Evalue=3e-57,
Organism=Homo sapiens, GI42516563, Length=345, Percent_Identity=23.1884057971014, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI4504031, Length=297, Percent_Identity=23.5690235690236, Blast_Score=66, Evalue=6e-11,
Organism=Escherichia coli, GI1788353, Length=361, Percent_Identity=97.5069252077562, Blast_Score=729, Evalue=0.0,
Organism=Escherichia coli, GI48994969, Length=353, Percent_Identity=73.9376770538244, Blast_Score=545, Evalue=1e-156,
Organism=Escherichia coli, GI1786974, Length=356, Percent_Identity=25.561797752809, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1788366, Length=385, Percent_Identity=24.4155844155844, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17568069, Length=335, Percent_Identity=35.2238805970149, Blast_Score=203, Evalue=1e-52,
Organism=Caenorhabditis elegans, GI115532424, Length=347, Percent_Identity=34.0057636887608, Blast_Score=177, Evalue=6e-45,
Organism=Caenorhabditis elegans, GI71982035, Length=374, Percent_Identity=26.7379679144385, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71982038, Length=376, Percent_Identity=26.5957446808511, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17539532, Length=338, Percent_Identity=23.0769230769231, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6319493, Length=368, Percent_Identity=26.9021739130435, Blast_Score=90, Evalue=5e-19,
Organism=Drosophila melanogaster, GI19923002, Length=367, Percent_Identity=25.8855585831063, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21356223, Length=338, Percent_Identity=23.6686390532544, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24158427, Length=345, Percent_Identity=23.4782608695652, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 40573; Mature: 40573

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILVTGGAGFIGSAVVRHIINNRQDSVVNVDKLTYAGNLESLADVSDSERYVFEHADIC
CEEEEECCCCHHHHHHHHHHHCCCCCCEEEHHHEEECCCHHHHHCCCCCCCEEEHHCCHH
DAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIVGTYVLLEAARNYWSALDS
HHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
DKKNSFRFHHISTDEVYGDLPHPDEVNIKEGLPLFTETTAYAPSSPYSASKASSDHLVRA
CCCCCEEEEECCHHHHCCCCCCCCCCCHHCCCCCEECCCCCCCCCCCCCCCCCHHHHHHH
WKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEGKGLPIYGKGDQIRDWLYVEDHA
HHHHCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCEEEEEHHC
RALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDLLDEIVPKEKSYREQITYVTDRPGH
CEEEEEEECCCCCCEEECCCCCCCCCCHHEEHHHHHHHHHCCCCHHHHHHEEEEECCCCC
DRRYAIDAEKIGRELGWKPQETFESGIRKTVEWYLSNTKWVDNVKSGAYQSWIEQNYEGR
CCEEEECHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCC
Q
C
>Mature Secondary Structure
MKILVTGGAGFIGSAVVRHIINNRQDSVVNVDKLTYAGNLESLADVSDSERYVFEHADIC
CEEEEECCCCHHHHHHHHHHHCCCCCCEEEHHHEEECCCHHHHHCCCCCCCEEEHHCCHH
DAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIVGTYVLLEAARNYWSALDS
HHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
DKKNSFRFHHISTDEVYGDLPHPDEVNIKEGLPLFTETTAYAPSSPYSASKASSDHLVRA
CCCCCEEEEECCHHHHCCCCCCCCCCCHHCCCCCEECCCCCCCCCCCCCCCCCHHHHHHH
WKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEGKGLPIYGKGDQIRDWLYVEDHA
HHHHCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCEEEEEHHC
RALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDLLDEIVPKEKSYREQITYVTDRPGH
CEEEEEEECCCCCCEEECCCCCCCCCCHHEEHHHHHHHHHCCCCHHHHHHEEEEECCCCC
DRRYAIDAEKIGRELGWKPQETFESGIRKTVEWYLSNTKWVDNVKSGAYQSWIEQNYEGR
CCEEEECHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCC
Q
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7517391; 9097040; 9278503 [H]