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The map label for this gene is gdhA [H]

Identifier: 187736530

GI number: 187736530

Start: 2496479

End: 2497834

Strand: Direct

Name: gdhA [H]

Synonym: Amuc_2051

Alternate gene names: 187736530

Gene position: 2496479-2497834 (Clockwise)

Preceding gene: 187736529

Following gene: 187736532

Centisome position: 93.71

GC content: 58.92

Gene sequence:

>1356_bases
ATGGCACAGACATATTCCCAGCGCGTGCTCGACTTGGTTAAGGCCAAGCATAGCCACGAAAAAGAATTCATCCAGGCCGT
TCAGGAAGTGCTCAGCACGATCGAACCGGTTTTGGCCGCGAATTCCAAGTATGAAGACCACGCGATTCTGGAACGGATCG
TGGAACCGGAAAGAACCATCCTGTTCCGCGTGCCGTGGATCGATGACCAGGGCAAGGTTCAGGTGAACCGCGGATACCGT
GTGGAATTCAACAGCGCCATCGGACCATACAAGGGCGGCCTCCGCTTCCATCCCAGCGTCAACCTCGGCATCCTGAAGTT
TCTGGGTTTTGAACAGGTTTTCAAAAACTCCCTCACCACCCTCCCCATGGGCGGCGGCAAGGGCGGTTCCGACTTCGACC
CCAAGGGCAAGAGCGATAATGAAGTGATGCGCTTCTGCCAGAGTTTCATGACGGAGCTTCAGCGCCACATCGGCGCGGAT
ACGGACGTTCCCGCGGGGGACATCGGCGTGGGCGGCCGTGAAATCGGCTACCTGTTCGGACAATACAAGAGAATCCGCAA
CGAATTCACCGGCGTTCTCACCGGCAAGAGCCTGAACTGGGGCGGCTCCCTCATCCGCCCGGAAGCCACCGGCTACGGTT
GCGTATACTTCGCCCAGAACATGCTCGCCACCCGCAACGACGACTTGCAGGGCAAAGTCTGCCTGGTGTCCGGTTCCGGC
AATGTGGCCCAGTACACCGTGGAAAAACTTAACGAACTGGGCGCGAAAGCGGTCACGATGTCCGACTCCAACGGCTATAT
TTACGATCCGGACGGCATTTCCCCGGAAAAGCTGGCCTGGGTCATGGAACTCAAGAACAAGCGCCGCGGACGCATTGCGG
AATACGTGAAGCAATTCCCGAAAGCCCAGTACTTTGAAGGCCAGCGTCCCTGGAGCGTTCCGTGCGACTGCGCCTTCCCC
TCCGCCACGCAGAATGAAATCAACGGGGAAGACGCCCGCACCCTCATCAAGAACGGCTGCACCCTGGTAGCGGAAGGCGC
CAACATGCCTACGGATCTGGAAGGCATTGAAACCTACCTGGCCGCCAAAATCCTGTACGGCCCGGCCAAGGCCGCCAATG
CCGGCGGTGTGGCCACCTCCGGCCTGGAAATGTCCCAGAACAGCCAGCGCCTCTCCTGGACGCGTGAAGAAGTGGACCAC
AAGCTGAAGACCATCATGGCCAACATCCACGCAAACGCCGTGGCCCACGCCCGTGAATACTCCTCCGACAAATCCTTCAC
CAACTACGTCACCGGCGCCAACATCGCCGGCTTCGTGAAAGTGGCGGATTCCATGATCGACCAGGGGGTAGTCTGA

Upstream 100 bases:

>100_bases
GGAAAAAAATGATTGCCAGTCCGCTTCGCGGAATGATAAACGGAAGGCGCGTGCGAAAAACTGCGCGTAAAGCGTCAACC
CAAATACAACAACAGAAAAG

Downstream 100 bases:

>100_bases
CCCCGGCCGACATACTTGCTGCCAATGTAAAACACACCAGAAGCGCCGGGTTCGCCCGGCGCTTCTCTTTTCTGCGGAAA
CGTCTCCACTCCGGAAGTTC

Product: glutamate dehydrogenase

Products: NA

Alternate protein names: NADP-GDH [H]

Number of amino acids: Translated: 451; Mature: 450

Protein sequence:

>451_residues
MAQTYSQRVLDLVKAKHSHEKEFIQAVQEVLSTIEPVLAANSKYEDHAILERIVEPERTILFRVPWIDDQGKVQVNRGYR
VEFNSAIGPYKGGLRFHPSVNLGILKFLGFEQVFKNSLTTLPMGGGKGGSDFDPKGKSDNEVMRFCQSFMTELQRHIGAD
TDVPAGDIGVGGREIGYLFGQYKRIRNEFTGVLTGKSLNWGGSLIRPEATGYGCVYFAQNMLATRNDDLQGKVCLVSGSG
NVAQYTVEKLNELGAKAVTMSDSNGYIYDPDGISPEKLAWVMELKNKRRGRIAEYVKQFPKAQYFEGQRPWSVPCDCAFP
SATQNEINGEDARTLIKNGCTLVAEGANMPTDLEGIETYLAAKILYGPAKAANAGGVATSGLEMSQNSQRLSWTREEVDH
KLKTIMANIHANAVAHAREYSSDKSFTNYVTGANIAGFVKVADSMIDQGVV

Sequences:

>Translated_451_residues
MAQTYSQRVLDLVKAKHSHEKEFIQAVQEVLSTIEPVLAANSKYEDHAILERIVEPERTILFRVPWIDDQGKVQVNRGYR
VEFNSAIGPYKGGLRFHPSVNLGILKFLGFEQVFKNSLTTLPMGGGKGGSDFDPKGKSDNEVMRFCQSFMTELQRHIGAD
TDVPAGDIGVGGREIGYLFGQYKRIRNEFTGVLTGKSLNWGGSLIRPEATGYGCVYFAQNMLATRNDDLQGKVCLVSGSG
NVAQYTVEKLNELGAKAVTMSDSNGYIYDPDGISPEKLAWVMELKNKRRGRIAEYVKQFPKAQYFEGQRPWSVPCDCAFP
SATQNEINGEDARTLIKNGCTLVAEGANMPTDLEGIETYLAAKILYGPAKAANAGGVATSGLEMSQNSQRLSWTREEVDH
KLKTIMANIHANAVAHAREYSSDKSFTNYVTGANIAGFVKVADSMIDQGVV
>Mature_450_residues
AQTYSQRVLDLVKAKHSHEKEFIQAVQEVLSTIEPVLAANSKYEDHAILERIVEPERTILFRVPWIDDQGKVQVNRGYRV
EFNSAIGPYKGGLRFHPSVNLGILKFLGFEQVFKNSLTTLPMGGGKGGSDFDPKGKSDNEVMRFCQSFMTELQRHIGADT
DVPAGDIGVGGREIGYLFGQYKRIRNEFTGVLTGKSLNWGGSLIRPEATGYGCVYFAQNMLATRNDDLQGKVCLVSGSGN
VAQYTVEKLNELGAKAVTMSDSNGYIYDPDGISPEKLAWVMELKNKRRGRIAEYVKQFPKAQYFEGQRPWSVPCDCAFPS
ATQNEINGEDARTLIKNGCTLVAEGANMPTDLEGIETYLAAKILYGPAKAANAGGVATSGLEMSQNSQRLSWTREEVDHK
LKTIMANIHANAVAHAREYSSDKSFTNYVTGANIAGFVKVADSMIDQGVV

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=359, Percent_Identity=30.9192200557103, Blast_Score=158, Evalue=1e-38,
Organism=Homo sapiens, GI4885281, Length=359, Percent_Identity=30.3621169916435, Blast_Score=154, Evalue=1e-37,
Organism=Escherichia coli, GI1788059, Length=452, Percent_Identity=59.9557522123894, Blast_Score=556, Evalue=1e-159,
Organism=Caenorhabditis elegans, GI17544676, Length=367, Percent_Identity=28.8828337874659, Blast_Score=131, Evalue=8e-31,
Organism=Saccharomyces cerevisiae, GI6324951, Length=452, Percent_Identity=52.8761061946903, Blast_Score=440, Evalue=1e-124,
Organism=Saccharomyces cerevisiae, GI6319256, Length=456, Percent_Identity=52.4122807017544, Blast_Score=434, Evalue=1e-122,
Organism=Drosophila melanogaster, GI45553475, Length=408, Percent_Identity=29.1666666666667, Blast_Score=146, Evalue=3e-35,
Organism=Drosophila melanogaster, GI45549226, Length=408, Percent_Identity=29.1666666666667, Blast_Score=145, Evalue=4e-35,
Organism=Drosophila melanogaster, GI24649283, Length=413, Percent_Identity=28.3292978208232, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI24649460, Length=296, Percent_Identity=30.4054054054054, Blast_Score=123, Evalue=3e-28,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.4 [H]

Molecular weight: Translated: 49567; Mature: 49435

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQTYSQRVLDLVKAKHSHEKEFIQAVQEVLSTIEPVLAANSKYEDHAILERIVEPERTI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEE
LFRVPWIDDQGKVQVNRGYRVEFNSAIGPYKGGLRFHPSVNLGILKFLGFEQVFKNSLTT
EEEECCCCCCCCEEECCCEEEEECCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHCCCCE
LPMGGGKGGSDFDPKGKSDNEVMRFCQSFMTELQRHIGADTDVPAGDIGVGGREIGYLFG
ECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHH
QYKRIRNEFTGVLTGKSLNWGGSLIRPEATGYGCVYFAQNMLATRNDDLQGKVCLVSGSG
HHHHHHHHHCEEEECCCCCCCCCEECCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEECCC
NVAQYTVEKLNELGAKAVTMSDSNGYIYDPDGISPEKLAWVMELKNKRRGRIAEYVKQFP
CHHHHHHHHHHHCCCEEEEEECCCCEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCC
KAQYFEGQRPWSVPCDCAFPSATQNEINGEDARTLIKNGCTLVAEGANMPTDLEGIETYL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEEECCCCCCCCHHHHHHHH
AAKILYGPAKAANAGGVATSGLEMSQNSQRLSWTREEVDHKLKTIMANIHANAVAHAREY
HHHHHHCCCCCCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SSDKSFTNYVTGANIAGFVKVADSMIDQGVV
CCCCCHHHHCCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AQTYSQRVLDLVKAKHSHEKEFIQAVQEVLSTIEPVLAANSKYEDHAILERIVEPERTI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEE
LFRVPWIDDQGKVQVNRGYRVEFNSAIGPYKGGLRFHPSVNLGILKFLGFEQVFKNSLTT
EEEECCCCCCCCEEECCCEEEEECCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHCCCCE
LPMGGGKGGSDFDPKGKSDNEVMRFCQSFMTELQRHIGADTDVPAGDIGVGGREIGYLFG
ECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHH
QYKRIRNEFTGVLTGKSLNWGGSLIRPEATGYGCVYFAQNMLATRNDDLQGKVCLVSGSG
HHHHHHHHHCEEEECCCCCCCCCEECCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEECCC
NVAQYTVEKLNELGAKAVTMSDSNGYIYDPDGISPEKLAWVMELKNKRRGRIAEYVKQFP
CHHHHHHHHHHHCCCEEEEEECCCCEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCC
KAQYFEGQRPWSVPCDCAFPSATQNEINGEDARTLIKNGCTLVAEGANMPTDLEGIETYL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEEECCCCCCCCHHHHHHHH
AAKILYGPAKAANAGGVATSGLEMSQNSQRLSWTREEVDHKLKTIMANIHANAVAHAREY
HHHHHHCCCCCCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SSDKSFTNYVTGANIAGFVKVADSMIDQGVV
CCCCCHHHHCCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]