Click here to switch to the map view.

The map label for this gene is ynfJ [C]

Identifier: 187736528

GI number: 187736528

Start: 2492611

End: 2494401

Strand: Direct

Name: ynfJ [C]

Synonym: Amuc_2049

Alternate gene names: 187736528

Gene position: 2492611-2494401 (Clockwise)

Preceding gene: 187736527

Following gene: 187736529

Centisome position: 93.56

GC content: 61.42

Gene sequence:

>1791_bases
ATGACGGACAAGAAAAACCAGCCTGCGGAAAAGACGCGCCTTTCCTGGTCATGGCTGCGCATGGCGTTGAGCCTGCGTGA
AAAAATCAAGCTGGGGGACAAGCAGGTCATCTTCATCTGGGCGGTCGTCGTCGGAGCGCTGGGTGCCCTGATGGCCCTTG
TCTTTGAAATGGGCGTGGAACTGGTGCAGGACGTCCTGACGGGGCGGACCAGCTACAGGCAAATTCAGGTCTTTCAGGAA
ATGGCCAACCAGGACCCGGCCTGGTGCATCTTCGTTCCGGCGGCGGGCGGCCTTTTGGCGGGGCTTACGCTTCTCTTCAC
CCACCGCTTTGTGCCGGCCAAGGCGACGGAATACATGGAAGCCGTGGCGCTGGGCAATGGATACGTGCCTCCCAAGCCCA
GCCTTCTGCGTTCCCTGTCCGCCGTTTTCTCCGTAGGTTCCGGCGCATCCATCGGCCGTGAAGGCCCGCTGGTGCAGGCC
GCCGCCGTAGCGGGCTCCGCCCTGGGGCGTTTCTTCCACCTCTCAGCTCCTCGTCTGCGCCTGGTAGTGGCCTGCGCCGC
CGCCTCCGGCATGTCCTCCGCCTTCCATACGCCCCTGGCGGGCGGTCTGTTCGTAAGTGAAATCGTTCTGGGCGCGCTGA
CTATTGATTTCCTGGCTCCCCTGCTGGTTGCCAGCTGCGCGGGGTACTTTACTATGGGCCTGTTCCATGAACCCGCCCCC
ATCTACCAGCTCCAGCAGGAAGTTTCTCTGGCGGGCAATCAGCATGTGCTCTGGTGCGTGCTGCTGGGAGCCCTGGCCTC
CCTGGTCGCCAGTTTCTGGCTCCTCATCCTCAAAAAATCGCGCCGGTACCTGAACGGCAAGCGCCAATGGCTGCCCGTGC
GCCTGACGGCGGCAGGCGTGCTGGTGGGAGCCATCGCCATCTTTTACCCGGAAATCGTAGGGAACGGCAAAAATATCATC
ACCTCCCTCATTCATTATGAATTCGACGCCGCGAGAGCCGCCGTCCTGCTGTTCCTGAAAATATTCACCGTCGCCATCGT
CTTCGGCGTGGGAACCGTGGGGGGCGCCCTGACCCCCAGTCTGACTATCGGGAGCGTCTGCGGCTTCCTGTTCAGCGCGG
CTCTTACCCAGCTGGGGGTGCCCGGGGACCATGCCATCGCCTATTCCCTGGTGGGCATGGCCGCCTTTTTCACTACAGCT
GCGAACGCCCCGATCACCTCCCTGGTGCTGGTGGTGGAATTCACCATGGCCGGGCAAATGATGTTCCCCCTGATCATCGG
CGTGCTGGTTTCCTATGGAATGGCCAGGCTCACCAAAGCCCAGTCCATGTACCATGATTCCCTGGCCTTCGGCCCCAGAA
GCACCTTTGACAAACCCCTGGCCCAGGTACAGCTTCAGGATGTAGCGCGCAAGGATCCGCCCGTGGTGCATCCCCTGGAC
AAATTCGGCACCATTGCCGCCATGCTCATTAAAAACCCGGCCCAGCCCATCTTCGTCACTTCCCCGGCCGGCAAATACCT
GGGAAGCGTGGTGGCGGAAGACGTGGCGGCCTTTGCCCGCAACAAGGAGCTGGCCCAGGCCGTACTGGCCATGGATGTAC
TGCGCAGCGACATGCCTACGCTTCCCGCGGACATGCACCTCCCGGAAGCCCTGGGTATTTTCTCCCGCCCCCACTGCGCG
GAATCCCTGGCCCTTGTGAACCCGAACAACAATCTGCTGCTGGGCGTAGTCAACAAGACGGACCTCTACCTAGTGCTCTC
TGAAATCATGCGCAGGGAAAAACTGCAATAA

Upstream 100 bases:

>100_bases
AGAAATTGCTGCGGTGGCATAAAAACGCCCCCCTGGGCCCCAATGGGGAATGGACGGGAGATGAAGCCAGTCTCCTGGCG
TCCCTTCAACAAGCCCTGCA

Downstream 100 bases:

>100_bases
AATCTGCCTTGCCGTGCTGTATCCGTTGAAATACCGGAAAAAACGCCGCTCCCTTCCGCGGTTTGCGGGAAATGCCCGTT
CAAGGCCGAACACCCTTCCC

Product: Chloride channel core

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 596; Mature: 595

Protein sequence:

>596_residues
MTDKKNQPAEKTRLSWSWLRMALSLREKIKLGDKQVIFIWAVVVGALGALMALVFEMGVELVQDVLTGRTSYRQIQVFQE
MANQDPAWCIFVPAAGGLLAGLTLLFTHRFVPAKATEYMEAVALGNGYVPPKPSLLRSLSAVFSVGSGASIGREGPLVQA
AAVAGSALGRFFHLSAPRLRLVVACAAASGMSSAFHTPLAGGLFVSEIVLGALTIDFLAPLLVASCAGYFTMGLFHEPAP
IYQLQQEVSLAGNQHVLWCVLLGALASLVASFWLLILKKSRRYLNGKRQWLPVRLTAAGVLVGAIAIFYPEIVGNGKNII
TSLIHYEFDAARAAVLLFLKIFTVAIVFGVGTVGGALTPSLTIGSVCGFLFSAALTQLGVPGDHAIAYSLVGMAAFFTTA
ANAPITSLVLVVEFTMAGQMMFPLIIGVLVSYGMARLTKAQSMYHDSLAFGPRSTFDKPLAQVQLQDVARKDPPVVHPLD
KFGTIAAMLIKNPAQPIFVTSPAGKYLGSVVAEDVAAFARNKELAQAVLAMDVLRSDMPTLPADMHLPEALGIFSRPHCA
ESLALVNPNNNLLLGVVNKTDLYLVLSEIMRREKLQ

Sequences:

>Translated_596_residues
MTDKKNQPAEKTRLSWSWLRMALSLREKIKLGDKQVIFIWAVVVGALGALMALVFEMGVELVQDVLTGRTSYRQIQVFQE
MANQDPAWCIFVPAAGGLLAGLTLLFTHRFVPAKATEYMEAVALGNGYVPPKPSLLRSLSAVFSVGSGASIGREGPLVQA
AAVAGSALGRFFHLSAPRLRLVVACAAASGMSSAFHTPLAGGLFVSEIVLGALTIDFLAPLLVASCAGYFTMGLFHEPAP
IYQLQQEVSLAGNQHVLWCVLLGALASLVASFWLLILKKSRRYLNGKRQWLPVRLTAAGVLVGAIAIFYPEIVGNGKNII
TSLIHYEFDAARAAVLLFLKIFTVAIVFGVGTVGGALTPSLTIGSVCGFLFSAALTQLGVPGDHAIAYSLVGMAAFFTTA
ANAPITSLVLVVEFTMAGQMMFPLIIGVLVSYGMARLTKAQSMYHDSLAFGPRSTFDKPLAQVQLQDVARKDPPVVHPLD
KFGTIAAMLIKNPAQPIFVTSPAGKYLGSVVAEDVAAFARNKELAQAVLAMDVLRSDMPTLPADMHLPEALGIFSRPHCA
ESLALVNPNNNLLLGVVNKTDLYLVLSEIMRREKLQ
>Mature_595_residues
TDKKNQPAEKTRLSWSWLRMALSLREKIKLGDKQVIFIWAVVVGALGALMALVFEMGVELVQDVLTGRTSYRQIQVFQEM
ANQDPAWCIFVPAAGGLLAGLTLLFTHRFVPAKATEYMEAVALGNGYVPPKPSLLRSLSAVFSVGSGASIGREGPLVQAA
AVAGSALGRFFHLSAPRLRLVVACAAASGMSSAFHTPLAGGLFVSEIVLGALTIDFLAPLLVASCAGYFTMGLFHEPAPI
YQLQQEVSLAGNQHVLWCVLLGALASLVASFWLLILKKSRRYLNGKRQWLPVRLTAAGVLVGAIAIFYPEIVGNGKNIIT
SLIHYEFDAARAAVLLFLKIFTVAIVFGVGTVGGALTPSLTIGSVCGFLFSAALTQLGVPGDHAIAYSLVGMAAFFTTAA
NAPITSLVLVVEFTMAGQMMFPLIIGVLVSYGMARLTKAQSMYHDSLAFGPRSTFDKPLAQVQLQDVARKDPPVVHPLDK
FGTIAAMLIKNPAQPIFVTSPAGKYLGSVVAEDVAAFARNKELAQAVLAMDVLRSDMPTLPADMHLPEALGIFSRPHCAE
SLALVNPNNNLLLGVVNKTDLYLVLSEIMRREKLQ

Specific function: Unknown

COG id: COG0038

COG function: function code P; Chloride channel protein EriC

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chloride channel (TC 2.A.49) family. ClcB subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081943, Length=343, Percent_Identity=30.0291545189504, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI1786350, Length=329, Percent_Identity=24.6200607902736, Blast_Score=66, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014743
- InterPro:   IPR001807 [H]

Pfam domain/function: PF00654 Voltage_CLC [H]

EC number: NA

Molecular weight: Translated: 63794; Mature: 63663

Theoretical pI: Translated: 9.37; Mature: 9.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDKKNQPAEKTRLSWSWLRMALSLREKIKLGDKQVIFIWAVVVGALGALMALVFEMGVE
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LVQDVLTGRTSYRQIQVFQEMANQDPAWCIFVPAAGGLLAGLTLLFTHRFVPAKATEYME
HHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHCCCHHHHHHHH
AVALGNGYVPPKPSLLRSLSAVFSVGSGASIGREGPLVQAAAVAGSALGRFFHLSAPRLR
HHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHH
LVVACAAASGMSSAFHTPLAGGLFVSEIVLGALTIDFLAPLLVASCAGYFTMGLFHEPAP
HHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
IYQLQQEVSLAGNQHVLWCVLLGALASLVASFWLLILKKSRRYLNGKRQWLPVRLTAAGV
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHH
LVGAIAIFYPEIVGNGKNIITSLIHYEFDAARAAVLLFLKIFTVAIVFGVGTVGGALTPS
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
LTIGSVCGFLFSAALTQLGVPGDHAIAYSLVGMAAFFTTAANAPITSLVLVVEFTMAGQM
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
MFPLIIGVLVSYGMARLTKAQSMYHDSLAFGPRSTFDKPLAQVQLQDVARKDPPVVHPLD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHH
KFGTIAAMLIKNPAQPIFVTSPAGKYLGSVVAEDVAAFARNKELAQAVLAMDVLRSDMPT
HHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LPADMHLPEALGIFSRPHCAESLALVNPNNNLLLGVVNKTDLYLVLSEIMRREKLQ
CCCCCCCHHHHCCCCCCCHHCCEEEECCCCCEEEEEECCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TDKKNQPAEKTRLSWSWLRMALSLREKIKLGDKQVIFIWAVVVGALGALMALVFEMGVE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LVQDVLTGRTSYRQIQVFQEMANQDPAWCIFVPAAGGLLAGLTLLFTHRFVPAKATEYME
HHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHCCCHHHHHHHH
AVALGNGYVPPKPSLLRSLSAVFSVGSGASIGREGPLVQAAAVAGSALGRFFHLSAPRLR
HHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHH
LVVACAAASGMSSAFHTPLAGGLFVSEIVLGALTIDFLAPLLVASCAGYFTMGLFHEPAP
HHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
IYQLQQEVSLAGNQHVLWCVLLGALASLVASFWLLILKKSRRYLNGKRQWLPVRLTAAGV
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHH
LVGAIAIFYPEIVGNGKNIITSLIHYEFDAARAAVLLFLKIFTVAIVFGVGTVGGALTPS
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
LTIGSVCGFLFSAALTQLGVPGDHAIAYSLVGMAAFFTTAANAPITSLVLVVEFTMAGQM
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
MFPLIIGVLVSYGMARLTKAQSMYHDSLAFGPRSTFDKPLAQVQLQDVARKDPPVVHPLD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHH
KFGTIAAMLIKNPAQPIFVTSPAGKYLGSVVAEDVAAFARNKELAQAVLAMDVLRSDMPT
HHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LPADMHLPEALGIFSRPHCAESLALVNPNNNLLLGVVNKTDLYLVLSEIMRREKLQ
CCCCCCCHHHHCCCCCCCHHCCEEEECCCCCEEEEEECCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]