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The map label for this gene is thiE [H]

Identifier: 187736494

GI number: 187736494

Start: 2447996

End: 2448610

Strand: Direct

Name: thiE [H]

Synonym: Amuc_2015

Alternate gene names: 187736494

Gene position: 2447996-2448610 (Clockwise)

Preceding gene: 187736493

Following gene: 187736495

Centisome position: 91.89

GC content: 60.98

Gene sequence:

>615_bases
ATGAAAAAATTCAATCTCCATCTCTACCTGGTTACGGATGAAGCATCCAAATGCCGCCTCAGCCTTCTGGAAACCGTACG
GAAAGCGGCGGACGGAGGAGTTACCATCGTGCAGTACCGCTCCACCAATCCGGATGCGGGAACCTGTTACCGGGAAGCTT
TGCCCATACGGGATTTCCTGGCTTCCCGCGGTATTCCCTTCATCGTCAACAACCGCATTGACCTGGCTCTGGCGCTGGAA
GCAGACGGCGTCCACATCGGCCAGAGGGATCTTCCGGTTCCATCCGTCAGAGCCATGATCGGCCCGGATAAAATCCTGGG
ACTGTCCGTCTCCAATAAGGAACAGCTCCGCGCCGTGGATGCCTCCCTGGTGGACTACCTGGGCATGGGCCCCGTCTTCC
CCACCATTTCCAAGCTGAACGCGCCTCCCGTGCTGGGCGTGGAAGGCTTCGCCGCTCTGGCTTCCCAATCTCCCCTGCCC
GTTGTCGCCATCGGCGGACTGGACGCGGAACGGGCCCGTCAAGTGCGCGCCACGGGAACCGCCTCCGGAATTGCCGTTGT
CTCCGCCATTTGCGGAGCGGAGAATCCGGAAACCGCCGCACGGGCTCTTGCCTGA

Upstream 100 bases:

>100_bases
GGAGAATGAAGGAAAGACCATCGTCGTCCTGCTGCCGGACACGGGGGACCGCTATCTTTCCACCCCTCTGTTTACGGACT
GATTCCTTCCATTCCCTGCC

Downstream 100 bases:

>100_bases
CGGACGTACCGCAGGAAATACCGGACAATCAAGCACTTTTTTACGCCATGCTTTCATCGACAGACCTTGTTCAAGCCGTT
ACCGCCGATTTGGGAAAAAT

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 204; Mature: 204

Protein sequence:

>204_residues
MKKFNLHLYLVTDEASKCRLSLLETVRKAADGGVTIVQYRSTNPDAGTCYREALPIRDFLASRGIPFIVNNRIDLALALE
ADGVHIGQRDLPVPSVRAMIGPDKILGLSVSNKEQLRAVDASLVDYLGMGPVFPTISKLNAPPVLGVEGFAALASQSPLP
VVAIGGLDAERARQVRATGTASGIAVVSAICGAENPETAARALA

Sequences:

>Translated_204_residues
MKKFNLHLYLVTDEASKCRLSLLETVRKAADGGVTIVQYRSTNPDAGTCYREALPIRDFLASRGIPFIVNNRIDLALALE
ADGVHIGQRDLPVPSVRAMIGPDKILGLSVSNKEQLRAVDASLVDYLGMGPVFPTISKLNAPPVLGVEGFAALASQSPLP
VVAIGGLDAERARQVRATGTASGIAVVSAICGAENPETAARALA
>Mature_204_residues
MKKFNLHLYLVTDEASKCRLSLLETVRKAADGGVTIVQYRSTNPDAGTCYREALPIRDFLASRGIPFIVNNRIDLALALE
ADGVHIGQRDLPVPSVRAMIGPDKILGLSVSNKEQLRAVDASLVDYLGMGPVFPTISKLNAPPVLGVEGFAALASQSPLP
VVAIGGLDAERARQVRATGTASGIAVVSAICGAENPETAARALA

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=138, Percent_Identity=35.5072463768116, Blast_Score=64, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6325042, Length=220, Percent_Identity=35, Blast_Score=114, Evalue=9e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 21444; Mature: 21444

Theoretical pI: Translated: 8.05; Mature: 8.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKFNLHLYLVTDEASKCRLSLLETVRKAADGGVTIVQYRSTNPDAGTCYREALPIRDFL
CCEEEEEEEEEECCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHCCHHHHH
ASRGIPFIVNNRIDLALALEADGVHIGQRDLPVPSVRAMIGPDKILGLSVSNKEQLRAVD
HHCCCCEEECCCEEEEEEEECCCEEECCCCCCCCHHHHHCCCCCEEEEECCCHHHHHHHH
ASLVDYLGMGPVFPTISKLNAPPVLGVEGFAALASQSPLPVVAIGGLDAERARQVRATGT
HHHHHHHCCCCCCCHHHHCCCCCEECCCHHHHHHCCCCCCEEEECCCCHHHHHHHHCCCC
ASGIAVVSAICGAENPETAARALA
CHHHHHHHHHHCCCCHHHHHHHCC
>Mature Secondary Structure
MKKFNLHLYLVTDEASKCRLSLLETVRKAADGGVTIVQYRSTNPDAGTCYREALPIRDFL
CCEEEEEEEEEECCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHCCHHHHH
ASRGIPFIVNNRIDLALALEADGVHIGQRDLPVPSVRAMIGPDKILGLSVSNKEQLRAVD
HHCCCCEEECCCEEEEEEEECCCEEECCCCCCCCHHHHHCCCCCEEEEECCCHHHHHHHH
ASLVDYLGMGPVFPTISKLNAPPVLGVEGFAALASQSPLPVVAIGGLDAERARQVRATGT
HHHHHHHCCCCCCCHHHHCCCCCEECCCHHHHHHCCCCCCEEEECCCCHHHHHHHHCCCC
ASGIAVVSAICGAENPETAARALA
CHHHHHHHHHHCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA