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The map label for this gene is cysK [H]

Identifier: 187736493

GI number: 187736493

Start: 2447048

End: 2447977

Strand: Direct

Name: cysK [H]

Synonym: Amuc_2014

Alternate gene names: 187736493

Gene position: 2447048-2447977 (Clockwise)

Preceding gene: 187736492

Following gene: 187736494

Centisome position: 91.85

GC content: 61.4

Gene sequence:

>930_bases
ATGAGAATTTACAAAAACATTACGGAACTGATCGGCGGCACCCCGTTGCTGGAACTGACCAATTACGAACGCAGGAATGG
CCTGAACGCCACGCTCCTGGCCAAGCTGGAATACCTGAACCCTGCCGGCAGCGTGAAAGACCGCATTGCCCAGGCCATGA
TTGATGCGGCGGAAGCCGCCGGGAAGCTGAAGCCGGATTCCGTCATCATTGAGCCCACCAGCGGAAACACGGGCATCGGC
CTGGCGGCGGTGGCCACTTCACGCGGGTACCGGATTATATTGACCATGCCGGAGACGATGAGCGTGGAACGCCGCAACCT
TCTGAAAGCCTACGGCGCGGAACTGGTGCTGACGGACGGCGCGCTGGGCATGAAGGGAGCCATCTCCAAGGCGGAGGAAC
TGGCCGCGGGACTGCCCAACAGCTTTATCCCCGGCCAGTTCGCCAACCCGTCCAACCCGGAAGCGCATTTCCGCACCACA
GGCCCGGAAATCTGGAATGATACAGAAGGCAAAGCGGATATTTTTGTGGCGGGCGTGGGTACCGGAGGCACCATCACAGG
CGTGGGCAAGTACCTTAAAACCCGTAATCCGGACATCCGGATCGTGGCTGTGGAGCCGTTCTCCTCCCCCGTGCTGACCC
AGGGCACGGCCGGCCCCCACAAGATTCAGGGCATTGGCGCGGGCTTCGTGCCGGGAACGCTGGACACCTCCGTGTACGAT
GAAGTCATCGCCGTTACCAATGAAGACGCTTTCTCCACAGGGAAGGAACTGGCGCGCACGGACGGCGTGCTGACGGGTAT
TTCCTCCGGAGCAGCTCTCTGGGCCGCCACACAGGTGGCGCGGCGCCCGGAGAATGAAGGAAAGACCATCGTCGTCCTGC
TGCCGGACACGGGGGACCGCTATCTTTCCACCCCTCTGTTTACGGACTGA

Upstream 100 bases:

>100_bases
CGGGACGGAACATATCGACGATATTCTGGAAGATCTGGAACAAGGCTTCCGCGCCATTCTCTAACGATTTAACGGCAGCA
ACGCTCTTTTCCTACAAGCC

Downstream 100 bases:

>100_bases
TTCCTTCCATTCCCTGCCATGAAAAAATTCAATCTCCATCTCTACCTGGTTACGGATGAAGCATCCAAATGCCGCCTCAG
CCTTCTGGAAACCGTACGGA

Product: cysteine synthase A

Products: NA

Alternate protein names: OAS sulfhydrylase; OASS; Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine-specific cysteine synthase; Sulfide-dependent cysteine synthase [H]

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MRIYKNITELIGGTPLLELTNYERRNGLNATLLAKLEYLNPAGSVKDRIAQAMIDAAEAAGKLKPDSVIIEPTSGNTGIG
LAAVATSRGYRIILTMPETMSVERRNLLKAYGAELVLTDGALGMKGAISKAEELAAGLPNSFIPGQFANPSNPEAHFRTT
GPEIWNDTEGKADIFVAGVGTGGTITGVGKYLKTRNPDIRIVAVEPFSSPVLTQGTAGPHKIQGIGAGFVPGTLDTSVYD
EVIAVTNEDAFSTGKELARTDGVLTGISSGAALWAATQVARRPENEGKTIVVLLPDTGDRYLSTPLFTD

Sequences:

>Translated_309_residues
MRIYKNITELIGGTPLLELTNYERRNGLNATLLAKLEYLNPAGSVKDRIAQAMIDAAEAAGKLKPDSVIIEPTSGNTGIG
LAAVATSRGYRIILTMPETMSVERRNLLKAYGAELVLTDGALGMKGAISKAEELAAGLPNSFIPGQFANPSNPEAHFRTT
GPEIWNDTEGKADIFVAGVGTGGTITGVGKYLKTRNPDIRIVAVEPFSSPVLTQGTAGPHKIQGIGAGFVPGTLDTSVYD
EVIAVTNEDAFSTGKELARTDGVLTGISSGAALWAATQVARRPENEGKTIVVLLPDTGDRYLSTPLFTD
>Mature_309_residues
MRIYKNITELIGGTPLLELTNYERRNGLNATLLAKLEYLNPAGSVKDRIAQAMIDAAEAAGKLKPDSVIIEPTSGNTGIG
LAAVATSRGYRIILTMPETMSVERRNLLKAYGAELVLTDGALGMKGAISKAEELAAGLPNSFIPGQFANPSNPEAHFRTT
GPEIWNDTEGKADIFVAGVGTGGTITGVGKYLKTRNPDIRIVAVEPFSSPVLTQGTAGPHKIQGIGAGFVPGTLDTSVYD
EVIAVTNEDAFSTGKELARTDGVLTGISSGAALWAATQVARRPENEGKTIVVLLPDTGDRYLSTPLFTD

Specific function: Catalyzes the conversion of O-acetylserine (OAS) to cysteine through the elimination of acetate and addition of hydrogen sulfide [H]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=310, Percent_Identity=42.258064516129, Blast_Score=234, Evalue=1e-61,
Organism=Homo sapiens, GI295821200, Length=310, Percent_Identity=42.258064516129, Blast_Score=234, Evalue=1e-61,
Organism=Homo sapiens, GI4557415, Length=310, Percent_Identity=42.258064516129, Blast_Score=234, Evalue=1e-61,
Organism=Escherichia coli, GI1788754, Length=317, Percent_Identity=55.205047318612, Blast_Score=300, Evalue=8e-83,
Organism=Escherichia coli, GI2367138, Length=301, Percent_Identity=43.8538205980066, Blast_Score=226, Evalue=2e-60,
Organism=Caenorhabditis elegans, GI17535051, Length=302, Percent_Identity=54.3046357615894, Blast_Score=336, Evalue=1e-92,
Organism=Caenorhabditis elegans, GI17562970, Length=300, Percent_Identity=53, Blast_Score=324, Evalue=4e-89,
Organism=Caenorhabditis elegans, GI32566674, Length=293, Percent_Identity=53.5836177474403, Blast_Score=317, Evalue=4e-87,
Organism=Caenorhabditis elegans, GI115535073, Length=308, Percent_Identity=55.1948051948052, Blast_Score=309, Evalue=1e-84,
Organism=Caenorhabditis elegans, GI17561720, Length=299, Percent_Identity=51.505016722408, Blast_Score=288, Evalue=2e-78,
Organism=Caenorhabditis elegans, GI32566672, Length=214, Percent_Identity=53.2710280373832, Blast_Score=241, Evalue=3e-64,
Organism=Caenorhabditis elegans, GI17534315, Length=320, Percent_Identity=39.375, Blast_Score=190, Evalue=7e-49,
Organism=Caenorhabditis elegans, GI25147552, Length=322, Percent_Identity=36.0248447204969, Blast_Score=175, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI17561716, Length=119, Percent_Identity=45.3781512605042, Blast_Score=110, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6321594, Length=315, Percent_Identity=40.9523809523809, Blast_Score=218, Evalue=1e-57,
Organism=Saccharomyces cerevisiae, GI6321449, Length=329, Percent_Identity=35.8662613981763, Blast_Score=138, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24643623, Length=316, Percent_Identity=39.5569620253165, Blast_Score=203, Evalue=1e-52,
Organism=Drosophila melanogaster, GI20129101, Length=316, Percent_Identity=39.5569620253165, Blast_Score=203, Evalue=1e-52,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001216
- InterPro:   IPR005856
- InterPro:   IPR005859
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =2.5.1.47 [H]

Molecular weight: Translated: 32528; Mature: 32528

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00901 CYS_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIYKNITELIGGTPLLELTNYERRNGLNATLLAKLEYLNPAGSVKDRIAQAMIDAAEAA
CCHHHHHHHHHCCCCEEEECCCHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHC
GKLKPDSVIIEPTSGNTGIGLAAVATSRGYRIILTMPETMSVERRNLLKAYGAELVLTDG
CCCCCCCEEEEECCCCCCCEEEEEECCCCCEEEEECCCCCCHHHHHHHHHCCCEEEEECC
ALGMKGAISKAEELAAGLPNSFIPGQFANPSNPEAHFRTTGPEIWNDTEGKADIFVAGVG
CCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCEEEEEEECC
TGGTITGVGKYLKTRNPDIRIVAVEPFSSPVLTQGTAGPHKIQGIGAGFVPGTLDTSVYD
CCCCHHHHHHHHHCCCCCEEEEEECCCCCCCEECCCCCCCEEEECCCCCCCCCCCHHHHH
EVIAVTNEDAFSTGKELARTDGVLTGISSGAALWAATQVARRPENEGKTIVVLLPDTGDR
HEEEEECCCHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCC
YLSTPLFTD
EECCCCCCC
>Mature Secondary Structure
MRIYKNITELIGGTPLLELTNYERRNGLNATLLAKLEYLNPAGSVKDRIAQAMIDAAEAA
CCHHHHHHHHHCCCCEEEECCCHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHC
GKLKPDSVIIEPTSGNTGIGLAAVATSRGYRIILTMPETMSVERRNLLKAYGAELVLTDG
CCCCCCCEEEEECCCCCCCEEEEEECCCCCEEEEECCCCCCHHHHHHHHHCCCEEEEECC
ALGMKGAISKAEELAAGLPNSFIPGQFANPSNPEAHFRTTGPEIWNDTEGKADIFVAGVG
CCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCEEEEEEECC
TGGTITGVGKYLKTRNPDIRIVAVEPFSSPVLTQGTAGPHKIQGIGAGFVPGTLDTSVYD
CCCCHHHHHHHHHCCCCCEEEEEECCCCCCCEECCCCCCCEEEECCCCCCCCCCCHHHHH
EVIAVTNEDAFSTGKELARTDGVLTGISSGAALWAATQVARRPENEGKTIVVLLPDTGDR
HEEEEECCCHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCC
YLSTPLFTD
EECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]