Click here to switch to the map view.

The map label for this gene is pheS [H]

Identifier: 187736479

GI number: 187736479

Start: 2428414

End: 2429439

Strand: Reverse

Name: pheS [H]

Synonym: Amuc_1999

Alternate gene names: 187736479

Gene position: 2429439-2428414 (Counterclockwise)

Preceding gene: 187736480

Following gene: 187736478

Centisome position: 91.19

GC content: 62.18

Gene sequence:

>1026_bases
ATGAAGGAAGAGATTGTACGCATCCAACGGGACGCCCTGGCACGCATCGCCCAGGCGTCTGACAGGCGCGGCGTGGAAGA
CGCGCGCGTGGCCATCCTCGGCAAAAAAGGGGAACTGACCCTGGCCCAGACGGGCATGAAGGACGTTCCCAGAGAGGAAA
AGCCCGCTGTCGGCCAGTTGCTTAACGAGGCCCGCAAGGCCATTACGGAAGCTCTGGACGCCAAGCTGGAGGAAGTGCAG
GCCCAGGCGGACAAGGCCGCCGTGGCCGGTGTGGACCTGACGCTTCCGGCCCGCTCCCTGCCGCCTGGCGGTCTGCACCC
CCTCACCATCGTTAGGGATGAAGCCGTCCGCATTCTGCGCCACATGGGCTTTGCCCTGGCGGACGGCCCGGAGATTGAGG
ACGAGTTCCACTGCTTTGACGCGCTGAACACGCCGGAAGATCACCCGGCCCGCAATGAGAAGGATACATTTTACTTTGAT
TCCGGCAAGCTTCTGCGTACGCACACGTCTTCCGTGCAGATCCGCTCCATGGAAAAGCAGCTGCCGCCCGTGCGTGTCAT
CGCTCCCGGTTCCGCCTACCGCCGCGACGAAATTGACGCCACGCACCTTTCCGCCTTCAATCAGCTTGAAGGCCTGTATG
TGGATACGGACGTTTCCGTGGGCGACCTGAAAGGAACGCTGGAATATTTTCTGCGCGCCCTTTTCGGTTCCGGAACGGAG
GTGCGCTTCCGCCCCCATTTCTTCCCGTTCACGGAACCCAGCTTTGAAATTGACGTCAAGCTGAAGGTGGACGGCCAGGC
CCCCCGCTGGGTGGAGATTGCCGGCTGCGGCATGGTGGATCCCAATGTTTTTGAAGCCGTGGACCGCGAACTGGGCCTGG
ACCCCGGAGCGCAGGCCCGCTACACGGGGCTGACCGGCTTTGCCTTCGGCATCGGCCTGGACCGCCTGGCGATGATCCGC
TGGGGCATCAGGGACATTCGCGCCCTGATTGAGAATGATGTGCGCTTCCTTGCCCAATTCCAATAA

Upstream 100 bases:

>100_bases
CCTTTCCGGGGTCCGCCATTTTCTTTTGAGTGACCTCCCCGCGGCCCGAAGGGCTTGACGCTGGCGGGGGGGACGGGTAA
GCTCTTGCCCGTCAAATTTT

Downstream 100 bases:

>100_bases
TTTTTACCTGTTTTCCCGTTATGAAGATTTCCCTTAACTGGCTTTCCCAGTACATTGACCTGTCCGGCCTGAGCGTGGAT
GAAATGTCTGACATGCTGAC

Product: phenylalanyl-tRNA synthetase, alpha subunit

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS [H]

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MKEEIVRIQRDALARIAQASDRRGVEDARVAILGKKGELTLAQTGMKDVPREEKPAVGQLLNEARKAITEALDAKLEEVQ
AQADKAAVAGVDLTLPARSLPPGGLHPLTIVRDEAVRILRHMGFALADGPEIEDEFHCFDALNTPEDHPARNEKDTFYFD
SGKLLRTHTSSVQIRSMEKQLPPVRVIAPGSAYRRDEIDATHLSAFNQLEGLYVDTDVSVGDLKGTLEYFLRALFGSGTE
VRFRPHFFPFTEPSFEIDVKLKVDGQAPRWVEIAGCGMVDPNVFEAVDRELGLDPGAQARYTGLTGFAFGIGLDRLAMIR
WGIRDIRALIENDVRFLAQFQ

Sequences:

>Translated_341_residues
MKEEIVRIQRDALARIAQASDRRGVEDARVAILGKKGELTLAQTGMKDVPREEKPAVGQLLNEARKAITEALDAKLEEVQ
AQADKAAVAGVDLTLPARSLPPGGLHPLTIVRDEAVRILRHMGFALADGPEIEDEFHCFDALNTPEDHPARNEKDTFYFD
SGKLLRTHTSSVQIRSMEKQLPPVRVIAPGSAYRRDEIDATHLSAFNQLEGLYVDTDVSVGDLKGTLEYFLRALFGSGTE
VRFRPHFFPFTEPSFEIDVKLKVDGQAPRWVEIAGCGMVDPNVFEAVDRELGLDPGAQARYTGLTGFAFGIGLDRLAMIR
WGIRDIRALIENDVRFLAQFQ
>Mature_341_residues
MKEEIVRIQRDALARIAQASDRRGVEDARVAILGKKGELTLAQTGMKDVPREEKPAVGQLLNEARKAITEALDAKLEEVQ
AQADKAAVAGVDLTLPARSLPPGGLHPLTIVRDEAVRILRHMGFALADGPEIEDEFHCFDALNTPEDHPARNEKDTFYFD
SGKLLRTHTSSVQIRSMEKQLPPVRVIAPGSAYRRDEIDATHLSAFNQLEGLYVDTDVSVGDLKGTLEYFLRALFGSGTE
VRFRPHFFPFTEPSFEIDVKLKVDGQAPRWVEIAGCGMVDPNVFEAVDRELGLDPGAQARYTGLTGFAFGIGLDRLAMIR
WGIRDIRALIENDVRFLAQFQ

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5729820, Length=306, Percent_Identity=30.3921568627451, Blast_Score=128, Evalue=6e-30,
Organism=Homo sapiens, GI4758340, Length=279, Percent_Identity=30.1075268817204, Blast_Score=124, Evalue=1e-28,
Organism=Escherichia coli, GI1788007, Length=332, Percent_Identity=49.0963855421687, Blast_Score=332, Evalue=3e-92,
Organism=Caenorhabditis elegans, GI32566635, Length=252, Percent_Identity=34.1269841269841, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI32563657, Length=276, Percent_Identity=29.3478260869565, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17508957, Length=276, Percent_Identity=29.3478260869565, Blast_Score=119, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6321087, Length=279, Percent_Identity=29.7491039426523, Blast_Score=125, Evalue=9e-30,
Organism=Saccharomyces cerevisiae, GI6325304, Length=139, Percent_Identity=34.5323741007194, Blast_Score=74, Evalue=5e-14,
Organism=Drosophila melanogaster, GI18858079, Length=278, Percent_Identity=30.2158273381295, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI17137424, Length=293, Percent_Identity=26.962457337884, Blast_Score=106, Evalue=2e-23,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978 [H]

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 37835; Mature: 37835

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEEIVRIQRDALARIAQASDRRGVEDARVAILGKKGELTLAQTGMKDVPREEKPAVGQL
CCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCEEEECCCCHHCCCCCCCHHHHH
LNEARKAITEALDAKLEEVQAQADKAAVAGVDLTLPARSLPPGGLHPLTIVRDEAVRILR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCEEEEHHHHHHHHH
HMGFALADGPEIEDEFHCFDALNTPEDHPARNEKDTFYFDSGKLLRTHTSSVQIRSMEKQ
HCCCEECCCCCCCCHHHHHHCCCCCCCCCCCCCCCEEEECCCCEEEECCCCEEEEHHHHH
LPPVRVIAPGSAYRRDEIDATHLSAFNQLEGLYVDTDVSVGDLKGTLEYFLRALFGSGTE
CCCEEEECCCCCCCCCCCCHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHCCCCE
VRFRPHFFPFTEPSFEIDVKLKVDGQAPRWVEIAGCGMVDPNVFEAVDRELGLDPGAQAR
EEECCCCCCCCCCCEEEEEEEEECCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCCE
YTGLTGFAFGIGLDRLAMIRWGIRDIRALIENDVRFLAQFQ
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKEEIVRIQRDALARIAQASDRRGVEDARVAILGKKGELTLAQTGMKDVPREEKPAVGQL
CCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCEEEECCCCHHCCCCCCCHHHHH
LNEARKAITEALDAKLEEVQAQADKAAVAGVDLTLPARSLPPGGLHPLTIVRDEAVRILR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCEEEEHHHHHHHHH
HMGFALADGPEIEDEFHCFDALNTPEDHPARNEKDTFYFDSGKLLRTHTSSVQIRSMEKQ
HCCCEECCCCCCCCHHHHHHCCCCCCCCCCCCCCCEEEECCCCEEEECCCCEEEEHHHHH
LPPVRVIAPGSAYRRDEIDATHLSAFNQLEGLYVDTDVSVGDLKGTLEYFLRALFGSGTE
CCCEEEECCCCCCCCCCCCHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHCCCCE
VRFRPHFFPFTEPSFEIDVKLKVDGQAPRWVEIAGCGMVDPNVFEAVDRELGLDPGAQAR
EEECCCCCCCCCCCEEEEEEEEECCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCCE
YTGLTGFAFGIGLDRLAMIRWGIRDIRALIENDVRFLAQFQ
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA