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The map label for this gene is purD [H]

Identifier: 187736345

GI number: 187736345

Start: 2265147

End: 2266403

Strand: Reverse

Name: purD [H]

Synonym: Amuc_1862

Alternate gene names: 187736345

Gene position: 2266403-2265147 (Counterclockwise)

Preceding gene: 187736346

Following gene: 187736344

Centisome position: 85.07

GC content: 57.04

Gene sequence:

>1257_bases
ATGAAAATAGCCGTTATTGGAAAAGGTGGACGTGAACATGCACTGGTCAAGGCACTCAAGGAATCTCCCTCCGCTCCGGA
AATGTACTGTTTCCCGGGAAGTGACGCCATCAACCGTCTGGCTACCCCCATCCCGGCCAGGGATCTGCCCTCGCTGATCG
ACTGGATGGTTTCCAATAAAATGGATCTCTGCGTTGCCGGGGAGGAAAGCTATCTGGTCAAGGATGAAGGCCTGGCCAAT
GCCTGCTCCCGTGCGGGCATCCCCTGCTGGGGGCCGGTAAAAGAAAGCGCCCAGCTGGAAGCCAGCAAGGAATTTGCCAA
GGACTTCCTGCTCCGCCACCGCATCCCTACCGGGCAGGCGCGCGTAGCGGCCACATTGGAGGAAGCCCGGCAATTCATCG
GAAACAACTATCCTACCGTGCTGAAATTTGACGGTCTGGCCGCCGGAAAGGGCGTAGCCGTCTGCATGAGCAAGGAGGAA
GCAGACTCTTTCCTGAAAGAAGTATTTACGGACAGGCGTTTTGGTGAAGGGCGGCTGCTGGTGGAAGAATTTCTTACCGG
ACCGGAAGTTTCCATCTTCGGCGCTCTGGTGGACGACCATTATCTCATCCTCTGCCCGGCACGGGACTACAAGCGCCTCA
AGGAAGGCGATGCAGGCCCCAACACGGGCGGCATGGGAGCGGTGGCTTCCCGTCGGCTGGTGGAGCCGGAAATGATGGAA
CGCATTGAAAAGGAAATCGTGGCTCCGACGGTAGCCGGACTGAAAAAAGACGGACTGCCTTACCGGGGTTTTCTGTACTT
CGGCCTGATGCTGACTCCAAACGGCCCAAAAGTAATTGAATACAACTGCCGCTTCGGCGATCCGGAATGCCAGGCCGTTA
TGCCCCTGCTCTCCGGAGACTTGGCTTCCTTCTGCCTGCATGGGGCCAAGGGAGAATTTGCGCCGGAAGAAATTTCCTTC
CAAAACGGCTGGAGCGTCTGCTGCGTGCTGGCCTCCAAAGGATATCCGGAGACCTCCCATTCCGGCGACGCTATTCAGGG
ACTGGACGATATTTCAAATGCTTCCGTTTACCACGCCGGCACCAAATGGAATGCGGATAAAAACTGTTATGAAACCAATG
GCGGCCGCGTCCTGGCCGTCGTGGCACAGGGAGATACCCTCTCCGAAGCGCGCCAGCGTGCCCACAACGAAATCAAAAAG
ATTCAATTTCACGGCATGCAGCGCCGGCCGGACATCGGCTTTGCCAGCTTCGTCTGA

Upstream 100 bases:

>100_bases
GGCAATGGAGGAAAAGCATGGGAACAACCGGACAAAAGGGAAAACCTCACCGTTCATTTCAGCTTGGCAAAATCAGTCCG
TCCCGGCATACTGGCGGCGT

Downstream 100 bases:

>100_bases
TTTTTCCGCGTTTCCCTCCCCATCTGCCCTATGAAACCGGCGGCCCTTCTCATCCTCCTGTTTCTTTCCTGCCCTGCATG
CCTGATGGCGGACAACGCCG

Product: phosphoribosylamine/glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 418; Mature: 418

Protein sequence:

>418_residues
MKIAVIGKGGREHALVKALKESPSAPEMYCFPGSDAINRLATPIPARDLPSLIDWMVSNKMDLCVAGEESYLVKDEGLAN
ACSRAGIPCWGPVKESAQLEASKEFAKDFLLRHRIPTGQARVAATLEEARQFIGNNYPTVLKFDGLAAGKGVAVCMSKEE
ADSFLKEVFTDRRFGEGRLLVEEFLTGPEVSIFGALVDDHYLILCPARDYKRLKEGDAGPNTGGMGAVASRRLVEPEMME
RIEKEIVAPTVAGLKKDGLPYRGFLYFGLMLTPNGPKVIEYNCRFGDPECQAVMPLLSGDLASFCLHGAKGEFAPEEISF
QNGWSVCCVLASKGYPETSHSGDAIQGLDDISNASVYHAGTKWNADKNCYETNGGRVLAVVAQGDTLSEARQRAHNEIKK
IQFHGMQRRPDIGFASFV

Sequences:

>Translated_418_residues
MKIAVIGKGGREHALVKALKESPSAPEMYCFPGSDAINRLATPIPARDLPSLIDWMVSNKMDLCVAGEESYLVKDEGLAN
ACSRAGIPCWGPVKESAQLEASKEFAKDFLLRHRIPTGQARVAATLEEARQFIGNNYPTVLKFDGLAAGKGVAVCMSKEE
ADSFLKEVFTDRRFGEGRLLVEEFLTGPEVSIFGALVDDHYLILCPARDYKRLKEGDAGPNTGGMGAVASRRLVEPEMME
RIEKEIVAPTVAGLKKDGLPYRGFLYFGLMLTPNGPKVIEYNCRFGDPECQAVMPLLSGDLASFCLHGAKGEFAPEEISF
QNGWSVCCVLASKGYPETSHSGDAIQGLDDISNASVYHAGTKWNADKNCYETNGGRVLAVVAQGDTLSEARQRAHNEIKK
IQFHGMQRRPDIGFASFV
>Mature_418_residues
MKIAVIGKGGREHALVKALKESPSAPEMYCFPGSDAINRLATPIPARDLPSLIDWMVSNKMDLCVAGEESYLVKDEGLAN
ACSRAGIPCWGPVKESAQLEASKEFAKDFLLRHRIPTGQARVAATLEEARQFIGNNYPTVLKFDGLAAGKGVAVCMSKEE
ADSFLKEVFTDRRFGEGRLLVEEFLTGPEVSIFGALVDDHYLILCPARDYKRLKEGDAGPNTGGMGAVASRRLVEPEMME
RIEKEIVAPTVAGLKKDGLPYRGFLYFGLMLTPNGPKVIEYNCRFGDPECQAVMPLLSGDLASFCLHGAKGEFAPEEISF
QNGWSVCCVLASKGYPETSHSGDAIQGLDDISNASVYHAGTKWNADKNCYETNGGRVLAVVAQGDTLSEARQRAHNEIKK
IQFHGMQRRPDIGFASFV

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI28416899, Length=426, Percent_Identity=39.6713615023474, Blast_Score=273, Evalue=1e-73,
Organism=Homo sapiens, GI4503915, Length=426, Percent_Identity=39.6713615023474, Blast_Score=273, Evalue=2e-73,
Organism=Homo sapiens, GI209869995, Length=426, Percent_Identity=39.6713615023474, Blast_Score=273, Evalue=2e-73,
Organism=Homo sapiens, GI209869993, Length=426, Percent_Identity=39.6713615023474, Blast_Score=273, Evalue=2e-73,
Organism=Escherichia coli, GI1790438, Length=426, Percent_Identity=40.1408450704225, Blast_Score=296, Evalue=2e-81,
Organism=Caenorhabditis elegans, GI17567511, Length=425, Percent_Identity=35.7647058823529, Blast_Score=205, Evalue=4e-53,
Organism=Saccharomyces cerevisiae, GI6321203, Length=442, Percent_Identity=36.4253393665158, Blast_Score=263, Evalue=4e-71,
Organism=Drosophila melanogaster, GI62471619, Length=432, Percent_Identity=40.9722222222222, Blast_Score=273, Evalue=2e-73,
Organism=Drosophila melanogaster, GI24582400, Length=432, Percent_Identity=41.2037037037037, Blast_Score=273, Evalue=2e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 45534; Mature: 45534

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIAVIGKGGREHALVKALKESPSAPEMYCFPGSDAINRLATPIPARDLPSLIDWMVSNK
CEEEEECCCCCHHHHHHHHHHCCCCCEEEEECCCHHHHHHCCCCCCHHHHHHHHHHHCCC
MDLCVAGEESYLVKDEGLANACSRAGIPCWGPVKESAQLEASKEFAKDFLLRHRIPTGQA
CEEEEECCCCEEEECCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHH
RVAATLEEARQFIGNNYPTVLKFDGLAAGKGVAVCMSKEEADSFLKEVFTDRRFGEGRLL
HHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEECHHHHHHHHHHHHHCCCCCCCCCH
VEEFLTGPEVSIFGALVDDHYLILCPARDYKRLKEGDAGPNTGGMGAVASRRLVEPEMME
HHHHCCCCCEEEEEEEECCCEEEEECCHHHHHHHCCCCCCCCCCCCHHHHHHCCCHHHHH
RIEKEIVAPTVAGLKKDGLPYRGFLYFGLMLTPNGPKVIEYNCRFGDPECQAVMPLLSGD
HHHHHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCCEEEEEECCCCCCHHHHHHHHHHCH
LASFCLHGAKGEFAPEEISFQNGWSVCCVLASKGYPETSHSGDAIQGLDDISNASVYHAG
HHHHHHCCCCCCCCCHHCCCCCCCEEEEEEECCCCCCCCCCCCHHHCHHHCCCCEEEECC
TKWNADKNCYETNGGRVLAVVAQGDTLSEARQRAHNEIKKIQFHGMQRRPDIGFASFV
CCCCCCCCCEECCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCC
>Mature Secondary Structure
MKIAVIGKGGREHALVKALKESPSAPEMYCFPGSDAINRLATPIPARDLPSLIDWMVSNK
CEEEEECCCCCHHHHHHHHHHCCCCCEEEEECCCHHHHHHCCCCCCHHHHHHHHHHHCCC
MDLCVAGEESYLVKDEGLANACSRAGIPCWGPVKESAQLEASKEFAKDFLLRHRIPTGQA
CEEEEECCCCEEEECCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHH
RVAATLEEARQFIGNNYPTVLKFDGLAAGKGVAVCMSKEEADSFLKEVFTDRRFGEGRLL
HHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEECHHHHHHHHHHHHHCCCCCCCCCH
VEEFLTGPEVSIFGALVDDHYLILCPARDYKRLKEGDAGPNTGGMGAVASRRLVEPEMME
HHHHCCCCCEEEEEEEECCCEEEEECCHHHHHHHCCCCCCCCCCCCHHHHHHCCCHHHHH
RIEKEIVAPTVAGLKKDGLPYRGFLYFGLMLTPNGPKVIEYNCRFGDPECQAVMPLLSGD
HHHHHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCCEEEEEECCCCCCHHHHHHHHHHCH
LASFCLHGAKGEFAPEEISFQNGWSVCCVLASKGYPETSHSGDAIQGLDDISNASVYHAG
HHHHHHCCCCCCCCCHHCCCCCCCEEEEEEECCCCCCCCCCCCHHHCHHHCCCCEEEECC
TKWNADKNCYETNGGRVLAVVAQGDTLSEARQRAHNEIKKIQFHGMQRRPDIGFASFV
CCCCCCCCCEECCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]