Click here to switch to the map view.

The map label for this gene is dnaG [H]

Identifier: 187736114

GI number: 187736114

Start: 1957360

End: 1959159

Strand: Direct

Name: dnaG [H]

Synonym: Amuc_1625

Alternate gene names: 187736114

Gene position: 1957360-1959159 (Clockwise)

Preceding gene: 187736111

Following gene: 187736115

Centisome position: 73.47

GC content: 59.17

Gene sequence:

>1800_bases
ATGGGAAGGATTGATGAAGACAGTATCCGGCGCGTTCTGGAGGCCACTGACATTGTAGATGTGGTGGGATCCTATCTTCC
CCTGAAAAGGGCCGGTTCCCGGTGGAAGGCATGCTGCCCGTTCCACAATGAAAAGACGCCTTCCTTTATTGTGGACCAGA
GTCGGCAGAATTTTAAATGCTTTGGCTGCGGGGAAGGAGGGTCTGCCATCACTTTTGTGATGAAGATGGAGAACCTGGGA
TTTGCGGATACCGTGCGAAGGCTGGCGGAAAAAGCCGGAATCTCCATTGTGGAGGAGGTCTATGACGCAGCGGAGGAAAG
GCGGCGCAAGGCACGCACGGCTTTGCTGGTGGCCCACAAGAAGGCGGCTGGGTTTTTCCACAGGCTCCTGCTTTGTTCCC
CGAAGGCGGCGGAGGCCAGGGCTTACCTGAATTCCCGCGGATACGGGGCAGACATGGCGAAACGCTGGCAGGTAGGGTGG
GCACCTGACTCCTCGCGGGAGTTTCTGGCCTGGGCCCGCAGGGAGGGGATCCCGGATCAGGTGCTGATAGATTCCGGTCT
CGCCAACCGCGGAGAACGGGGAGATTTGTACGCCCGCTTCCGGGACAGGCTGATGTTCCCCATCAACAATGTGTACGGAG
AATGTGTGGCGTTTTCCGGCCGCATTCTGCGGCCTCAGGAAAACGTAGGCAAGTACGTTAATTCCCCGGAGACGGCCATT
TTCAAAAAGGGGGACGTGGTGTTTGCCCTGGACAAGGCGCGCGGCCCTATGGGAAAAAGCGGCAAAGCCCTGTTGTGCGA
GGGGCAGATAGACGTCATTGCCTGCCACGAGGCGGGTATTTCTTTTGCCGTAGCCCCTTTGGGGACGGCGTTTACTCCGG
AGCATGCCCGGATTCTTTCACGCTATGCTTCACGGATAGTGTTGTGTTTTGACGCGGACAAGGCGGGCATGGCTGCGGCG
GACCGGGCTTTCCGTGAACTGGCCCCTTTTGGCAAGGATATTTACCTGGTGAACCTTCCTGCCGGGGACGATCCGGATTC
TTTCATGAAGAGGGAGGGGAAAGAGGCTTTTTCTGGGATGGTGGAGCGGGCCCGTTCCTTCTTTGAGGTTCGCATTGAAC
GGGCCAGGGAGAGCGGTTTGTTGGATGACGCCGCTTCCAGCGCCGCTTTTGCCCGTGAGATGACGGCCCTGCTGGCCTGC
ATGAGCGATTCGGTGGCCCGGGACCTGGCTACGGCTGACGTAGCCACGCGCATGCGCATGGCTGCGGATAATTTGCGCGG
CGCCGTCAGAATGGCCGGACGCCGGAAGGGGCAGGAACAGGCCCAGTCCGCAGAGCGTAAGACGGCTGAGGAGGTTCCGC
AGCATCCTCCTGTGAAAATGGATCGCGCCGTGGCCGTGCTCTGTGAACTGGCCCTTCAGAATTCCCGTGCCCAGGGGCTT
ATCGTGGACCGCATTGAAGAATTGCTGGAGCCGATGCGGCTGCTGCAGGGCGGAGGCATCTTAAAAAAAATTCTGGCCAG
GCTGCCTTCTCCGGACAGCCCCGCGGCCGTTCAGGCTTTTCTGGTGTCTCTGCCTCAGCCGGAACGGGATGCCCTGGGCA
TGCTGAATCTGGAGCCCATACCTATTCCCGATGTGGACAGGAGCGTACAGGAAGCCTGCTCCGGCATTGCGAAGGCCGCT
TTGGAGAGGCACATCGCCTCCCTGATGGCGGAACTGGCGGATCCCTCCACGGATGCTGCCAGAAGGCTGGAATTGTCCAA
ACTTAGTGTTGACTTGAAGCGTTTGCTGGGTACAATGTAG

Upstream 100 bases:

>100_bases
CCGTCTTTTCCCTGCCTGTGGAGGCCTTGTTTCCTCGCATGGCGTTCCGATTCTGTATTCTTGTGCACCCGTACCCCTTG
TGATAGACTGGTTCCGCCTT

Downstream 100 bases:

>100_bases
TGCCTCGATACCTCCGGGTATTGATATAATGGTGAATTGTGCCCTCTTCTTTTCAAGATATGTCCAAATCCGGTGACCCA
TCCCCCTCTTCTCCCAAGAA

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 599; Mature: 598

Protein sequence:

>599_residues
MGRIDEDSIRRVLEATDIVDVVGSYLPLKRAGSRWKACCPFHNEKTPSFIVDQSRQNFKCFGCGEGGSAITFVMKMENLG
FADTVRRLAEKAGISIVEEVYDAAEERRRKARTALLVAHKKAAGFFHRLLLCSPKAAEARAYLNSRGYGADMAKRWQVGW
APDSSREFLAWARREGIPDQVLIDSGLANRGERGDLYARFRDRLMFPINNVYGECVAFSGRILRPQENVGKYVNSPETAI
FKKGDVVFALDKARGPMGKSGKALLCEGQIDVIACHEAGISFAVAPLGTAFTPEHARILSRYASRIVLCFDADKAGMAAA
DRAFRELAPFGKDIYLVNLPAGDDPDSFMKREGKEAFSGMVERARSFFEVRIERARESGLLDDAASSAAFAREMTALLAC
MSDSVARDLATADVATRMRMAADNLRGAVRMAGRRKGQEQAQSAERKTAEEVPQHPPVKMDRAVAVLCELALQNSRAQGL
IVDRIEELLEPMRLLQGGGILKKILARLPSPDSPAAVQAFLVSLPQPERDALGMLNLEPIPIPDVDRSVQEACSGIAKAA
LERHIASLMAELADPSTDAARRLELSKLSVDLKRLLGTM

Sequences:

>Translated_599_residues
MGRIDEDSIRRVLEATDIVDVVGSYLPLKRAGSRWKACCPFHNEKTPSFIVDQSRQNFKCFGCGEGGSAITFVMKMENLG
FADTVRRLAEKAGISIVEEVYDAAEERRRKARTALLVAHKKAAGFFHRLLLCSPKAAEARAYLNSRGYGADMAKRWQVGW
APDSSREFLAWARREGIPDQVLIDSGLANRGERGDLYARFRDRLMFPINNVYGECVAFSGRILRPQENVGKYVNSPETAI
FKKGDVVFALDKARGPMGKSGKALLCEGQIDVIACHEAGISFAVAPLGTAFTPEHARILSRYASRIVLCFDADKAGMAAA
DRAFRELAPFGKDIYLVNLPAGDDPDSFMKREGKEAFSGMVERARSFFEVRIERARESGLLDDAASSAAFAREMTALLAC
MSDSVARDLATADVATRMRMAADNLRGAVRMAGRRKGQEQAQSAERKTAEEVPQHPPVKMDRAVAVLCELALQNSRAQGL
IVDRIEELLEPMRLLQGGGILKKILARLPSPDSPAAVQAFLVSLPQPERDALGMLNLEPIPIPDVDRSVQEACSGIAKAA
LERHIASLMAELADPSTDAARRLELSKLSVDLKRLLGTM
>Mature_598_residues
GRIDEDSIRRVLEATDIVDVVGSYLPLKRAGSRWKACCPFHNEKTPSFIVDQSRQNFKCFGCGEGGSAITFVMKMENLGF
ADTVRRLAEKAGISIVEEVYDAAEERRRKARTALLVAHKKAAGFFHRLLLCSPKAAEARAYLNSRGYGADMAKRWQVGWA
PDSSREFLAWARREGIPDQVLIDSGLANRGERGDLYARFRDRLMFPINNVYGECVAFSGRILRPQENVGKYVNSPETAIF
KKGDVVFALDKARGPMGKSGKALLCEGQIDVIACHEAGISFAVAPLGTAFTPEHARILSRYASRIVLCFDADKAGMAAAD
RAFRELAPFGKDIYLVNLPAGDDPDSFMKREGKEAFSGMVERARSFFEVRIERARESGLLDDAASSAAFAREMTALLACM
SDSVARDLATADVATRMRMAADNLRGAVRMAGRRKGQEQAQSAERKTAEEVPQHPPVKMDRAVAVLCELALQNSRAQGLI
VDRIEELLEPMRLLQGGGILKKILARLPSPDSPAAVQAFLVSLPQPERDALGMLNLEPIPIPDVDRSVQEACSGIAKAAL
ERHIASLMAELADPSTDAARRLELSKLSVDLKRLLGTM

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=368, Percent_Identity=38.5869565217391, Blast_Score=262, Evalue=4e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 65661; Mature: 65530

Theoretical pI: Translated: 8.53; Mature: 8.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRIDEDSIRRVLEATDIVDVVGSYLPLKRAGSRWKACCPFHNEKTPSFIVDQSRQNFKC
CCCCCHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCEECCCCCCCCCCCEECCCCCCCEE
FGCGEGGSAITFVMKMENLGFADTVRRLAEKAGISIVEEVYDAAEERRRKARTALLVAHK
EEECCCCCEEEEEEECCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KAAGFFHRLLLCSPKAAEARAYLNSRGYGADMAKRWQVGWAPDSSREFLAWARREGIPDQ
HHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHCCCCCH
VLIDSGLANRGERGDLYARFRDRLMFPINNVYGECVAFSGRILRPQENVGKYVNSPETAI
HEECCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCEECCHHHHHHHCCCCCHHE
FKKGDVVFALDKARGPMGKSGKALLCEGQIDVIACHEAGISFAVAPLGTAFTPEHARILS
EECCCEEEEEECCCCCCCCCCCEEEEECCEEEEEEECCCCEEEEECCCCCCCHHHHHHHH
RYASRIVLCFDADKAGMAAADRAFRELAPFGKDIYLVNLPAGDDPDSFMKREGKEAFSGM
HHHCEEEEEEECCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHH
VERARSFFEVRIERARESGLLDDAASSAAFAREMTALLACMSDSVARDLATADVATRMRM
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AADNLRGAVRMAGRRKGQEQAQSAERKTAEEVPQHPPVKMDRAVAVLCELALQNSRAQGL
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCH
IVDRIEELLEPMRLLQGGGILKKILARLPSPDSPAAVQAFLVSLPQPERDALGMLNLEPI
HHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHCCCCCHHHCCCCCCCCC
PIPDVDRSVQEACSGIAKAALERHIASLMAELADPSTDAARRLELSKLSVDLKRLLGTM
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GRIDEDSIRRVLEATDIVDVVGSYLPLKRAGSRWKACCPFHNEKTPSFIVDQSRQNFKC
CCCCHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCEECCCCCCCCCCCEECCCCCCCEE
FGCGEGGSAITFVMKMENLGFADTVRRLAEKAGISIVEEVYDAAEERRRKARTALLVAHK
EEECCCCCEEEEEEECCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KAAGFFHRLLLCSPKAAEARAYLNSRGYGADMAKRWQVGWAPDSSREFLAWARREGIPDQ
HHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHCCCCCH
VLIDSGLANRGERGDLYARFRDRLMFPINNVYGECVAFSGRILRPQENVGKYVNSPETAI
HEECCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCEECCHHHHHHHCCCCCHHE
FKKGDVVFALDKARGPMGKSGKALLCEGQIDVIACHEAGISFAVAPLGTAFTPEHARILS
EECCCEEEEEECCCCCCCCCCCEEEEECCEEEEEEECCCCEEEEECCCCCCCHHHHHHHH
RYASRIVLCFDADKAGMAAADRAFRELAPFGKDIYLVNLPAGDDPDSFMKREGKEAFSGM
HHHCEEEEEEECCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHH
VERARSFFEVRIERARESGLLDDAASSAAFAREMTALLACMSDSVARDLATADVATRMRM
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AADNLRGAVRMAGRRKGQEQAQSAERKTAEEVPQHPPVKMDRAVAVLCELALQNSRAQGL
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCH
IVDRIEELLEPMRLLQGGGILKKILARLPSPDSPAAVQAFLVSLPQPERDALGMLNLEPI
HHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHCCCCCHHHCCCCCCCCC
PIPDVDRSVQEACSGIAKAALERHIASLMAELADPSTDAARRLELSKLSVDLKRLLGTM
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7961408; 11466286 [H]