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The map label for this gene is cysI [H]

Identifier: 187735787

GI number: 187735787

Start: 1569682

End: 1571322

Strand: Reverse

Name: cysI [H]

Synonym: Amuc_1294

Alternate gene names: 187735787

Gene position: 1571322-1569682 (Counterclockwise)

Preceding gene: 187735788

Following gene: 187735774

Centisome position: 58.98

GC content: 49.42

Gene sequence:

>1641_bases
ATGATTAAAGAAGGATACACATCCAACGAACGAATCAAGGAGGAAAGCGATTTCCTCAGAGGGAATATCTCTAACCTCCT
CCATGACGATAGCATTACCCATTTCCCGTCCGAAGAAGAATTTCTGCTGAAATTCCATGGAATCACCCAGCAGGATAACC
GAGATGAACGGCTTATCCGAAAGAAAAACGGACAGGGACGAAATTGGTTATTCATGTTGAGGTTACGCCTCCCCGGAGGC
CGTATCACACCCAGTCAATGGCTGGCTGCGGAAACGCTAAGCGACCAATTCGGTAACGGCACTCTTAAACTTACGACACG
CCAAGCCATTCAAATCCACGGAGTCGTCAAGCAGGATTTGAAACAGACCATGAAAGATATCCATCACGCGGCCATCACCA
CGATTGCCGCCAGTGGAGACGCCACACGCAATGTCATGGTTTCTCTGCACCCGGAAGATTCCGGCATCCATGAGACAGTC
TTCAATCAAGCCCAGGAACTCTCCCGAAAACTGGAACCGCAGACACATGCCTATCATGAAATATGGCTGGATGCCAAACG
AATCTACTCGGGCGAGGAGGAGGAGCCTCTGTACGGTCCCGGATATTTGCCGCGCAAATTTAAAATCGCTTTTACGCTTC
CCCCGGAAAATGATGTGGATGTATACGCCCAGGACTTGAGCTTCGTGGCTATTGAAAAAGAGGGAAAACTCCTGGGATAT
GATATCCTGGCCGGCGGAGGAATGGGCTATGCTTACGGCAATCCCGGATCTTTCCCCCGCCTTGCGGATATTATCGGATT
CTGTTTCCCGGAACAGGTGGAGGAAGTAGCCCGGCAGGTGCTTTTGATCCATAAAGAATTCAGCACCCGCAGCAACAGAA
AAACATCCCGGCTCAGGTACACCATTGCCGGTAAAGGACTGGGCTGGTTCACTAACGAACTTGCAACACGCCTTCCATTT
CCTCTTCAAGAGGCCAAACCTTTTTCATTATCTACGAACGGAGATGCCGCAGATGTTCCTGGCCGACAAACCATTGAAAT
TGAAGGCGGCCGCATCCAAAATTCAAACAGGCAACAGCTTAAAACCGCTTTTCACGAAATAGCTTCCATTCATCAAGGAG
ATTTTTTCATCACAGGTAATCAAAATCTGGTCATTGACGGCATCACACCAGATACTGCCGTGCGAATCAAATCCATCATT
AGGAAGTACAATCTTCTCCCCAACGATTCGGGACTCAGGCGCAATTCCTCCGCTTGTACCAGCCTGCCATTTTGTCCGCA
GGCTCTTACAGACTCCGAAAGACTACTTCCAAAACTGGTGGATGAACTGGAATCACAGTTAAAAGAACTGGGGCTATGGG
ACGAAGAAATTTCTATACGCATGACCGGGTGTCCCAACGGATGCTCCAGGCCGTATCTGGCAGAACTGGCTTTCGTCGGC
AGAGGTCCGGGCAAGTACAATATCTGGATGGGCGGTTCCAGAAGAGGAGACAGACTAGGCTTCGTCTATCAGGAATCTGT
ATCCGTAAAAGAAATCGTAGACGTGCTCCGACCAGTATTTGCACGTTTTGCGGCAGAACGCAACCAAGGGGAAGGATTCG
GTGACTGGAGCATCCGCTCATTACATCCGCAATCTTTATAA

Upstream 100 bases:

>100_bases
TCTCTTAGGCCTGCTCTCAGATAAACTCATCTTCTCCCCCCTGGAACAATCTATCTACAAACGCTGGGGGACAAATCTAG
CTTAACCCACAGAAAATATT

Downstream 100 bases:

>100_bases
AAGCATAAACATCCGGAGCCGTTTTTGCCGTAGACGGCTCCGGGACAAATCCTGCTATATTTGAAAATCAAAGAGATATT
CCAGAAAACAAAATCATGAA

Product: Sulfite reductase (NADPH)

Products: NA

Alternate protein names: SiR-HP; SiRHP [H]

Number of amino acids: Translated: 546; Mature: 546

Protein sequence:

>546_residues
MIKEGYTSNERIKEESDFLRGNISNLLHDDSITHFPSEEEFLLKFHGITQQDNRDERLIRKKNGQGRNWLFMLRLRLPGG
RITPSQWLAAETLSDQFGNGTLKLTTRQAIQIHGVVKQDLKQTMKDIHHAAITTIAASGDATRNVMVSLHPEDSGIHETV
FNQAQELSRKLEPQTHAYHEIWLDAKRIYSGEEEEPLYGPGYLPRKFKIAFTLPPENDVDVYAQDLSFVAIEKEGKLLGY
DILAGGGMGYAYGNPGSFPRLADIIGFCFPEQVEEVARQVLLIHKEFSTRSNRKTSRLRYTIAGKGLGWFTNELATRLPF
PLQEAKPFSLSTNGDAADVPGRQTIEIEGGRIQNSNRQQLKTAFHEIASIHQGDFFITGNQNLVIDGITPDTAVRIKSII
RKYNLLPNDSGLRRNSSACTSLPFCPQALTDSERLLPKLVDELESQLKELGLWDEEISIRMTGCPNGCSRPYLAELAFVG
RGPGKYNIWMGGSRRGDRLGFVYQESVSVKEIVDVLRPVFARFAAERNQGEGFGDWSIRSLHPQSL

Sequences:

>Translated_546_residues
MIKEGYTSNERIKEESDFLRGNISNLLHDDSITHFPSEEEFLLKFHGITQQDNRDERLIRKKNGQGRNWLFMLRLRLPGG
RITPSQWLAAETLSDQFGNGTLKLTTRQAIQIHGVVKQDLKQTMKDIHHAAITTIAASGDATRNVMVSLHPEDSGIHETV
FNQAQELSRKLEPQTHAYHEIWLDAKRIYSGEEEEPLYGPGYLPRKFKIAFTLPPENDVDVYAQDLSFVAIEKEGKLLGY
DILAGGGMGYAYGNPGSFPRLADIIGFCFPEQVEEVARQVLLIHKEFSTRSNRKTSRLRYTIAGKGLGWFTNELATRLPF
PLQEAKPFSLSTNGDAADVPGRQTIEIEGGRIQNSNRQQLKTAFHEIASIHQGDFFITGNQNLVIDGITPDTAVRIKSII
RKYNLLPNDSGLRRNSSACTSLPFCPQALTDSERLLPKLVDELESQLKELGLWDEEISIRMTGCPNGCSRPYLAELAFVG
RGPGKYNIWMGGSRRGDRLGFVYQESVSVKEIVDVLRPVFARFAAERNQGEGFGDWSIRSLHPQSL
>Mature_546_residues
MIKEGYTSNERIKEESDFLRGNISNLLHDDSITHFPSEEEFLLKFHGITQQDNRDERLIRKKNGQGRNWLFMLRLRLPGG
RITPSQWLAAETLSDQFGNGTLKLTTRQAIQIHGVVKQDLKQTMKDIHHAAITTIAASGDATRNVMVSLHPEDSGIHETV
FNQAQELSRKLEPQTHAYHEIWLDAKRIYSGEEEEPLYGPGYLPRKFKIAFTLPPENDVDVYAQDLSFVAIEKEGKLLGY
DILAGGGMGYAYGNPGSFPRLADIIGFCFPEQVEEVARQVLLIHKEFSTRSNRKTSRLRYTIAGKGLGWFTNELATRLPF
PLQEAKPFSLSTNGDAADVPGRQTIEIEGGRIQNSNRQQLKTAFHEIASIHQGDFFITGNQNLVIDGITPDTAVRIKSII
RKYNLLPNDSGLRRNSSACTSLPFCPQALTDSERLLPKLVDELESQLKELGLWDEEISIRMTGCPNGCSRPYLAELAFVG
RGPGKYNIWMGGSRRGDRLGFVYQESVSVKEIVDVLRPVFARFAAERNQGEGFGDWSIRSLHPQSL

Specific function: Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L- cysteine from sulfate [H]

COG id: COG0155

COG function: function code P; Sulfite reductase, beta subunit (hemoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Escherichia coli, GI1789122, Length=548, Percent_Identity=37.7737226277372, Blast_Score=412, Evalue=1e-116,
Organism=Saccharomyces cerevisiae, GI6322597, Length=573, Percent_Identity=41.7102966841187, Blast_Score=438, Evalue=1e-123,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011786
- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066 [H]

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr [H]

EC number: =1.8.1.2 [H]

Molecular weight: Translated: 61403; Mature: 61403

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKEGYTSNERIKEESDFLRGNISNLLHDDSITHFPSEEEFLLKFHGITQQDNRDERLIR
CCCCCCCCCHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHH
KKNGQGRNWLFMLRLRLPGGRITPSQWLAAETLSDQFGNGTLKLTTRQAIQIHGVVKQDL
HCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCEEEEHHHHHHHH
KQTMKDIHHAAITTIAASGDATRNVMVSLHPEDSGIHETVFNQAQELSRKLEPQTHAYHE
HHHHHHHHHHHHHHEECCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHH
IWLDAKRIYSGEEEEPLYGPGYLPRKFKIAFTLPPENDVDVYAQDLSFVAIEKEGKLLGY
HHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCEEECCCEEEEEECCCCEEEE
DILAGGGMGYAYGNPGSFPRLADIIGFCFPEQVEEVARQVLLIHKEFSTRSNRKTSRLRY
EEEECCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEE
TIAGKGLGWFTNELATRLPFPLQEAKPFSLSTNGDAADVPGRQTIEIEGGRIQNSNRQQL
EEECCCCCHHHHHHHHHCCCCHHHCCCCEECCCCCCCCCCCCEEEEEECCEECCCCHHHH
KTAFHEIASIHQGDFFITGNQNLVIDGITPDTAVRIKSIIRKYNLLPNDSGLRRNSSACT
HHHHHHHHHHCCCCEEEECCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
SLPFCPQALTDSERLLPKLVDELESQLKELGLWDEEISIRMTGCPNGCSRPYLAELAFVG
CCCCCCHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCHHHHHHHCC
RGPGKYNIWMGGSRRGDRLGFVYQESVSVKEIVDVLRPVFARFAAERNQGEGFGDWSIRS
CCCCEEEEEECCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEC
LHPQSL
CCCCCC
>Mature Secondary Structure
MIKEGYTSNERIKEESDFLRGNISNLLHDDSITHFPSEEEFLLKFHGITQQDNRDERLIR
CCCCCCCCCHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHH
KKNGQGRNWLFMLRLRLPGGRITPSQWLAAETLSDQFGNGTLKLTTRQAIQIHGVVKQDL
HCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCEEEEHHHHHHHH
KQTMKDIHHAAITTIAASGDATRNVMVSLHPEDSGIHETVFNQAQELSRKLEPQTHAYHE
HHHHHHHHHHHHHHEECCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHH
IWLDAKRIYSGEEEEPLYGPGYLPRKFKIAFTLPPENDVDVYAQDLSFVAIEKEGKLLGY
HHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCEEECCCEEEEEECCCCEEEE
DILAGGGMGYAYGNPGSFPRLADIIGFCFPEQVEEVARQVLLIHKEFSTRSNRKTSRLRY
EEEECCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEE
TIAGKGLGWFTNELATRLPFPLQEAKPFSLSTNGDAADVPGRQTIEIEGGRIQNSNRQQL
EEECCCCCHHHHHHHHHCCCCHHHCCCCEECCCCCCCCCCCCEEEEEECCEECCCCHHHH
KTAFHEIASIHQGDFFITGNQNLVIDGITPDTAVRIKSIIRKYNLLPNDSGLRRNSSACT
HHHHHHHHHHCCCCEEEECCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
SLPFCPQALTDSERLLPKLVDELESQLKELGLWDEEISIRMTGCPNGCSRPYLAELAFVG
CCCCCCHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCHHHHHHHCC
RGPGKYNIWMGGSRRGDRLGFVYQESVSVKEIVDVLRPVFARFAAERNQGEGFGDWSIRS
CCCCEEEEEECCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEC
LHPQSL
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376 [H]