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The map label for this gene is yaeI [H]

Identifier: 187735773

GI number: 187735773

Start: 1541420

End: 1542271

Strand: Reverse

Name: yaeI [H]

Synonym: Amuc_1279

Alternate gene names: 187735773

Gene position: 1542271-1541420 (Counterclockwise)

Preceding gene: 187735774

Following gene: 187735772

Centisome position: 57.89

GC content: 56.34

Gene sequence:

>852_bases
ATGACCACCCACCCTTTCTCCAAAAAGCGTTCCCAGCTTCGCAAGCTCCTGTATGCGGCCGCGGCTGTCATTCTGCTGGG
GTCCCTACTGGCCTCCTACATGCATTGGGAAGCCAATCATCTGGAATTTAATGAAAACACGGCCCCTGCCGGATGTGTAC
CCGACCTTTCCGGCCTTCGTATCCTGGTATTGTCGGATATCCACACCAATCTGCCTCTGCTGGAAAAAGCGGCAACAATA
GCGGAACAGGCCAGACCGGACATGATCGTATTTCTGGGAGATCTGTACACGGATTTTCTGCGGGTCACTCATGCAGGCGA
CTACATCACCCAGATGAAACGCCTCTCCTCCGTCGCCCCGGCCTACGCATGTCTGGGCAACCATGACATGGCCCTTGCGG
ACAATGTGGAACGCGTCCTGAAGGAAGGGGGCTTCACACTGCTCAGGAATTCCGCCGCCTTCGTCTCCATCCCCAGGCTG
GGAAATGTGGAATTCAAACTGGTGGGGCTGGGAGACCTGCGGGAGGGTGACTTTTTCCCGGACCGGTGCATGAGCCCGCG
CGAATTGGAGGAAAACTCGCCCATGCCAACCATCGTCCTGAGCCACAACCCCAAAGGAAGGGAATTGCTGGGAAATTACC
GCTGGGACCTGATGCTCTCCGGCCATACGCATGGAGGCCAAATCAAACTGCCTTTCTTTTCCACCCCGCTATTGGCGTCG
GAAGGGGAAACCATGTACTCCGGGTTCCATCCTTATGAGGACAAGCAGGTATTCGTCACCCGCGGCATAGGCTATATAGG
GCCGGGCCGCTTCAACTGCCCGCCGGAAATCAACCTCATAACCATTCCCTAA

Upstream 100 bases:

>100_bases
AAGCGGCCGGGGGCGGAGATATTTACCTGGACGGGAAGCTGGTGCGCCGCAACGGATTATTCACCCTGCCGGAACTGGCC
ATTCTCAACCCCTCCCTATC

Downstream 100 bases:

>100_bases
CTTCCCGACCTGACCGCCATGGTTCTCTCTCTGCTGGCCTCCGGAGGTCCTAATGACGCGCCTCCATTCTTCATTCTGCT
TACTGTCGTCTTCATGGGAA

Product: phosphodiesterase YaeI

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 283; Mature: 282

Protein sequence:

>283_residues
MTTHPFSKKRSQLRKLLYAAAAVILLGSLLASYMHWEANHLEFNENTAPAGCVPDLSGLRILVLSDIHTNLPLLEKAATI
AEQARPDMIVFLGDLYTDFLRVTHAGDYITQMKRLSSVAPAYACLGNHDMALADNVERVLKEGGFTLLRNSAAFVSIPRL
GNVEFKLVGLGDLREGDFFPDRCMSPRELEENSPMPTIVLSHNPKGRELLGNYRWDLMLSGHTHGGQIKLPFFSTPLLAS
EGETMYSGFHPYEDKQVFVTRGIGYIGPGRFNCPPEINLITIP

Sequences:

>Translated_283_residues
MTTHPFSKKRSQLRKLLYAAAAVILLGSLLASYMHWEANHLEFNENTAPAGCVPDLSGLRILVLSDIHTNLPLLEKAATI
AEQARPDMIVFLGDLYTDFLRVTHAGDYITQMKRLSSVAPAYACLGNHDMALADNVERVLKEGGFTLLRNSAAFVSIPRL
GNVEFKLVGLGDLREGDFFPDRCMSPRELEENSPMPTIVLSHNPKGRELLGNYRWDLMLSGHTHGGQIKLPFFSTPLLAS
EGETMYSGFHPYEDKQVFVTRGIGYIGPGRFNCPPEINLITIP
>Mature_282_residues
TTHPFSKKRSQLRKLLYAAAAVILLGSLLASYMHWEANHLEFNENTAPAGCVPDLSGLRILVLSDIHTNLPLLEKAATIA
EQARPDMIVFLGDLYTDFLRVTHAGDYITQMKRLSSVAPAYACLGNHDMALADNVERVLKEGGFTLLRNSAAFVSIPRLG
NVEFKLVGLGDLREGDFFPDRCMSPRELEENSPMPTIVLSHNPKGRELLGNYRWDLMLSGHTHGGQIKLPFFSTPLLASE
GETMYSGFHPYEDKQVFVTRGIGYIGPGRFNCPPEINLITIP

Specific function: Shows phosphodiesterase activity, hydrolyzing phosphodiester bond in the artificial chromogenic substrate bis-p- nitrophenyl phosphate (bis-pNPP) [H]

COG id: COG1408

COG function: function code R; Predicted phosphohydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallophosphoesterase superfamily [H]

Homologues:

Organism=Escherichia coli, GI87081695, Length=286, Percent_Identity=32.5174825174825, Blast_Score=145, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004843 [H]

Pfam domain/function: PF00149 Metallophos [H]

EC number: NA

Molecular weight: Translated: 31400; Mature: 31268

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTHPFSKKRSQLRKLLYAAAAVILLGSLLASYMHWEANHLEFNENTAPAGCVPDLSGLR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCE
ILVLSDIHTNLPLLEKAATIAEQARPDMIVFLGDLYTDFLRVTHAGDYITQMKRLSSVAP
EEEEECCCCCCCHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHCCCHHHHHHHHHHHHCC
AYACLGNHDMALADNVERVLKEGGFTLLRNSAAFVSIPRLGNVEFKLVGLGDLREGDFFP
HHHHHCCCCCHHHHHHHHHHHCCCEEEEECCCEEEECCCCCCEEEEEEECCCCCCCCCCH
DRCMSPRELEENSPMPTIVLSHNPKGRELLGNYRWDLMLSGHTHGGQIKLPFFSTPLLAS
HHCCCCHHHHCCCCCCEEEEECCCCCHHHHCCCEEEEEEECCCCCCEEEECCCCCCCCCC
EGETMYSGFHPYEDKQVFVTRGIGYIGPGRFNCPPEINLITIP
CCCHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEECC
>Mature Secondary Structure 
TTHPFSKKRSQLRKLLYAAAAVILLGSLLASYMHWEANHLEFNENTAPAGCVPDLSGLR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCE
ILVLSDIHTNLPLLEKAATIAEQARPDMIVFLGDLYTDFLRVTHAGDYITQMKRLSSVAP
EEEEECCCCCCCHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHCCCHHHHHHHHHHHHCC
AYACLGNHDMALADNVERVLKEGGFTLLRNSAAFVSIPRLGNVEFKLVGLGDLREGDFFP
HHHHHCCCCCHHHHHHHHHHHCCCEEEEECCCEEEECCCCCCEEEEEEECCCCCCCCCCH
DRCMSPRELEENSPMPTIVLSHNPKGRELLGNYRWDLMLSGHTHGGQIKLPFFSTPLLAS
HHCCCCHHHHCCCCCCEEEEECCCCCHHHHCCCEEEEEEECCCCCCEEEECCCCCCCCCC
EGETMYSGFHPYEDKQVFVTRGIGYIGPGRFNCPPEINLITIP
CCCHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8202364; 9278503 [H]