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The map label for this gene is pckG [H]

Identifier: 187735704

GI number: 187735704

Start: 1445495

End: 1447321

Strand: Reverse

Name: pckG [H]

Synonym: Amuc_1210

Alternate gene names: 187735704

Gene position: 1447321-1445495 (Counterclockwise)

Preceding gene: 187735708

Following gene: 187735703

Centisome position: 54.33

GC content: 60.54

Gene sequence:

>1827_bases
ATGAGTACCACTCACGAAGCAGCTAAGAAGTGGGTCGAAGAAATCGCCCAGCTTTGCCAACCCGACTCCATCTACTGGTG
CAACGGCTCTCAGGAAGAAAATGACGAGCTTTGCAACATGATGGTAGAAAAGGGCACCTTTATCAAACTGAACCCCGAGA
AGCGCCCCGGGTGCTTCCTCGCCCGTTCCACCCCCTCCGATGTAGCCCGTGTGGAAGACCGCACGTTCATCTGCAGCGAG
AAGGAAGAAGACGCCGGCCCGACCAACCACTGGGCTGATCCCGTTGAAATGAAGGCCAAACTGAAGGGCCTCTTCAATGG
CTGCATGAAGGGCCGTACCATGTACGTGATTCCCTTCTGCATGGGTCCCCTGGACTCCCCCCTGGCCAAAATCGGCATTG
AAATCACGGACTCTCCCTATGTCGTGACCAACATGCGCATCATGACCAAGATGGGTGAACAGGTTCTCAAGCGCCTGGAA
GACGAAGTCAAGGGCGGCGGCAACCCGAAGCGTGACGGTTACGGGACATTCGTTCCCTGCCTCCACACCGTGGGCGCTCC
GCTTGCGGACGGCCAGGTTGACGTGCCGTGGCCCTGCAACGAAGAAAAGTACATCTGCCACTTCCCGGAAACGGAAGAAA
TCTGGTCCTTCGGTTCCGGTTACGGCGGCAACGCCCTGCTCGGCAAAAAGTGCCTGGCCCTGCGCATCGCCTCCGCCATC
GCCCGCAAGAACAAGTGGATGGCTGAACACATGCTCCTTCTGGGCATTGAATCCCCGGACGGCACCAAGGCCTACGTCGG
CGGCGCATTCCCGTCCGCCTGCGGCAAGACCAACCTGGCCATGCTCGTCCCCCCCGCCTCCTACCGGGAAGCCGGCTGGA
AAACCTCCATCATCGGTGACGACATCGCCTGGATCTGGCCCCATGAAGACGGCACCTTCCATGCCATCAACCCGGAAAAC
GGCTACTTCGGCGTGGCTCCCGGCACGTCCTACAAGACGAACCCGATCGCCATGGACACCATCAAGGAAAACGTCATCTT
CACCAACGTCGGTCTGACCGACGACGGCGACGTCTGGTGGGAAGGCATGGACGGCCCCGCTCCCGCCCACGCCATCGACT
GGCAGGGCAATGACTGGACCCCGGATTGCGGACGCAAGTGCGCTCATCCGAACGCCCGTTTCACCGCCCCCGCCTCCCAG
TGCCCGACGATTGATCCCGAATGGCAGAACCCGGAAGGCGTTTCCCTTTCCGCGATCCTGTTCGGCGGCCGCCGCGCCAC
CACCATGCCCCTCGTCTTCCAGTCCCGCGACTGGACGCACGGCGTTTACGTCGGTGCGACGATGGGTTCGGAAATGACCG
CCGCCGCCTTCGGCAACATCGGTCAGGTCCGCCGCGACCCGATGGCCATGCTTCCCTTCATCGGCTACCATGTGGGCGAC
TACTGGCAGCACTGGCTGGACATGGGCAAAACCGTTGCCAATCCTCCGCTGATCTTCAACGTGAACTGGTTCCGCAAGGA
CAAGAACGGCAACTTCATCTGGCCGGGCTACGGCGACAACATGCGCGTTCTCGACTGGATTCTGCGCCGCGCCAAGGGCA
AGGCTGAAGGCCAGAAGACCGTGCTGGGCGTCTCCCCGACCTATGCCGAACTGGATAAGACGGGCCTCGACTACAGCGAA
GAACAGTTCAACGCCAACATGGCTCTCAACCCGAGCGAATGGCAGGCTGAACTGGAAAGCCAGACCGAGCTGTTCGACAA
GGTGGGCAAAAAGCTCCCGGCCGAACTGGAAGAACAGCGCCAGCTTCTCATCAAGAGCTTTTCTTAA

Upstream 100 bases:

>100_bases
CCCGCATGCGGTTTCCATGCCCGGAGAAACAAAACCGCCCCATAAAAAGCCGCACGGGAAGCTTGCCAACGGCTATTTCC
GTGCATACACTATCTTCACC

Downstream 100 bases:

>100_bases
AAGTCCACCTGACTTAAGAAAAAACTCTTAAGGCAGAGGCCGCCCTTTAACAGGGCGGCCTCTTTTATTCCATAGCAACG
AATTTCAAATCCGTTTTACA

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase [H]

Number of amino acids: Translated: 608; Mature: 607

Protein sequence:

>608_residues
MSTTHEAAKKWVEEIAQLCQPDSIYWCNGSQEENDELCNMMVEKGTFIKLNPEKRPGCFLARSTPSDVARVEDRTFICSE
KEEDAGPTNHWADPVEMKAKLKGLFNGCMKGRTMYVIPFCMGPLDSPLAKIGIEITDSPYVVTNMRIMTKMGEQVLKRLE
DEVKGGGNPKRDGYGTFVPCLHTVGAPLADGQVDVPWPCNEEKYICHFPETEEIWSFGSGYGGNALLGKKCLALRIASAI
ARKNKWMAEHMLLLGIESPDGTKAYVGGAFPSACGKTNLAMLVPPASYREAGWKTSIIGDDIAWIWPHEDGTFHAINPEN
GYFGVAPGTSYKTNPIAMDTIKENVIFTNVGLTDDGDVWWEGMDGPAPAHAIDWQGNDWTPDCGRKCAHPNARFTAPASQ
CPTIDPEWQNPEGVSLSAILFGGRRATTMPLVFQSRDWTHGVYVGATMGSEMTAAAFGNIGQVRRDPMAMLPFIGYHVGD
YWQHWLDMGKTVANPPLIFNVNWFRKDKNGNFIWPGYGDNMRVLDWILRRAKGKAEGQKTVLGVSPTYAELDKTGLDYSE
EQFNANMALNPSEWQAELESQTELFDKVGKKLPAELEEQRQLLIKSFS

Sequences:

>Translated_608_residues
MSTTHEAAKKWVEEIAQLCQPDSIYWCNGSQEENDELCNMMVEKGTFIKLNPEKRPGCFLARSTPSDVARVEDRTFICSE
KEEDAGPTNHWADPVEMKAKLKGLFNGCMKGRTMYVIPFCMGPLDSPLAKIGIEITDSPYVVTNMRIMTKMGEQVLKRLE
DEVKGGGNPKRDGYGTFVPCLHTVGAPLADGQVDVPWPCNEEKYICHFPETEEIWSFGSGYGGNALLGKKCLALRIASAI
ARKNKWMAEHMLLLGIESPDGTKAYVGGAFPSACGKTNLAMLVPPASYREAGWKTSIIGDDIAWIWPHEDGTFHAINPEN
GYFGVAPGTSYKTNPIAMDTIKENVIFTNVGLTDDGDVWWEGMDGPAPAHAIDWQGNDWTPDCGRKCAHPNARFTAPASQ
CPTIDPEWQNPEGVSLSAILFGGRRATTMPLVFQSRDWTHGVYVGATMGSEMTAAAFGNIGQVRRDPMAMLPFIGYHVGD
YWQHWLDMGKTVANPPLIFNVNWFRKDKNGNFIWPGYGDNMRVLDWILRRAKGKAEGQKTVLGVSPTYAELDKTGLDYSE
EQFNANMALNPSEWQAELESQTELFDKVGKKLPAELEEQRQLLIKSFS
>Mature_607_residues
STTHEAAKKWVEEIAQLCQPDSIYWCNGSQEENDELCNMMVEKGTFIKLNPEKRPGCFLARSTPSDVARVEDRTFICSEK
EEDAGPTNHWADPVEMKAKLKGLFNGCMKGRTMYVIPFCMGPLDSPLAKIGIEITDSPYVVTNMRIMTKMGEQVLKRLED
EVKGGGNPKRDGYGTFVPCLHTVGAPLADGQVDVPWPCNEEKYICHFPETEEIWSFGSGYGGNALLGKKCLALRIASAIA
RKNKWMAEHMLLLGIESPDGTKAYVGGAFPSACGKTNLAMLVPPASYREAGWKTSIIGDDIAWIWPHEDGTFHAINPENG
YFGVAPGTSYKTNPIAMDTIKENVIFTNVGLTDDGDVWWEGMDGPAPAHAIDWQGNDWTPDCGRKCAHPNARFTAPASQC
PTIDPEWQNPEGVSLSAILFGGRRATTMPLVFQSRDWTHGVYVGATMGSEMTAAAFGNIGQVRRDPMAMLPFIGYHVGDY
WQHWLDMGKTVANPPLIFNVNWFRKDKNGNFIWPGYGDNMRVLDWILRRAKGKAEGQKTVLGVSPTYAELDKTGLDYSEE
QFNANMALNPSEWQAELESQTELFDKVGKKLPAELEEQRQLLIKSFS

Specific function: Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle [H]

COG id: COG1274

COG function: function code C; Phosphoenolpyruvate carboxykinase (GTP)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [GTP] family [H]

Homologues:

Organism=Homo sapiens, GI187281517, Length=622, Percent_Identity=48.0707395498392, Blast_Score=570, Evalue=1e-162,
Organism=Homo sapiens, GI66346721, Length=610, Percent_Identity=48.3606557377049, Blast_Score=567, Evalue=1e-161,
Organism=Homo sapiens, GI66346723, Length=390, Percent_Identity=50.5128205128205, Blast_Score=372, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI71989645, Length=614, Percent_Identity=45.6026058631922, Blast_Score=531, Evalue=1e-151,
Organism=Caenorhabditis elegans, GI71989658, Length=614, Percent_Identity=45.6026058631922, Blast_Score=531, Evalue=1e-151,
Organism=Caenorhabditis elegans, GI17555492, Length=624, Percent_Identity=44.7115384615385, Blast_Score=525, Evalue=1e-149,
Organism=Caenorhabditis elegans, GI71989653, Length=602, Percent_Identity=46.0132890365449, Blast_Score=523, Evalue=1e-149,
Organism=Caenorhabditis elegans, GI32564411, Length=567, Percent_Identity=47.0899470899471, Blast_Score=514, Evalue=1e-146,
Organism=Caenorhabditis elegans, GI32564407, Length=533, Percent_Identity=47.0919324577861, Blast_Score=479, Evalue=1e-135,
Organism=Caenorhabditis elegans, GI71989649, Length=495, Percent_Identity=47.8787878787879, Blast_Score=458, Evalue=1e-129,
Organism=Caenorhabditis elegans, GI32564409, Length=367, Percent_Identity=44.9591280653951, Blast_Score=320, Evalue=2e-87,
Organism=Caenorhabditis elegans, GI17540956, Length=588, Percent_Identity=23.8095238095238, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24655085, Length=606, Percent_Identity=50.6600660066007, Blast_Score=587, Evalue=1e-168,
Organism=Drosophila melanogaster, GI24655082, Length=610, Percent_Identity=50, Blast_Score=580, Evalue=1e-165,
Organism=Drosophila melanogaster, GI161077226, Length=576, Percent_Identity=50.8680555555556, Blast_Score=549, Evalue=1e-156,
Organism=Drosophila melanogaster, GI24655088, Length=508, Percent_Identity=52.3622047244094, Blast_Score=509, Evalue=1e-144,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018091
- InterPro:   IPR013035
- InterPro:   IPR008209
- InterPro:   IPR008210 [H]

Pfam domain/function: PF00821 PEPCK [H]

EC number: =4.1.1.32 [H]

Molecular weight: Translated: 67521; Mature: 67390

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS00505 PEPCK_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTTHEAAKKWVEEIAQLCQPDSIYWCNGSQEENDELCNMMVEKGTFIKLNPEKRPGCFL
CCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHCCCCEEEECCCCCCCEEE
ARSTPSDVARVEDRTFICSEKEEDAGPTNHWADPVEMKAKLKGLFNGCMKGRTMYVIPFC
ECCCCHHHHHHCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEEE
MGPLDSPLAKIGIEITDSPYVVTNMRIMTKMGEQVLKRLEDEVKGGGNPKRDGYGTFVPC
CCCCCCCHHHCCEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHH
LHTVGAPLADGQVDVPWPCNEEKYICHFPETEEIWSFGSGYGGNALLGKKCLALRIASAI
HHHCCCCCCCCEEECCCCCCCCCEEEECCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHH
ARKNKWMAEHMLLLGIESPDGTKAYVGGAFPSACGKTNLAMLVPPASYREAGWKTSIIGD
HHHHHHHHHEEEEEEECCCCCCEEEECCCCCCCCCCCCEEEEECCCHHHHCCCEEEECCC
DIAWIWPHEDGTFHAINPENGYFGVAPGTSYKTNPIAMDTIKENVIFTNVGLTDDGDVWW
CEEEEEECCCCCEEEECCCCCEEECCCCCCCCCCCEEHHHHCCCEEEEECCCCCCCCEEE
EGMDGPAPAHAIDWQGNDWTPDCGRKCAHPNARFTAPASQCPTIDPEWQNPEGVSLSAIL
ECCCCCCCCCEEECCCCCCCHHHHHHCCCCCCCCCCCHHHCCCCCCCCCCCCCCEEEEEE
FGGRRATTMPLVFQSRDWTHGVYVGATMGSEMTAAAFGNIGQVRRDPMAMLPFIGYHVGD
ECCCCCEEEEEEEECCCCCCCEEEEEECCCCHHHHHHCCCHHHHCCCHHHHHHHHHHHHH
YWQHWLDMGKTVANPPLIFNVNWFRKDKNGNFIWPGYGDNMRVLDWILRRAKGKAEGQKT
HHHHHHHHCHHHCCCCEEEEEEEEEECCCCCEECCCCCCCHHHHHHHHHHHCCCCCCCEE
VLGVSPTYAELDKTGLDYSEEQFNANMALNPSEWQAELESQTELFDKVGKKLPAELEEQR
EEECCCCHHHHHHCCCCCCHHHCCCCEECCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
QLLIKSFS
HHHHHCCC
>Mature Secondary Structure 
STTHEAAKKWVEEIAQLCQPDSIYWCNGSQEENDELCNMMVEKGTFIKLNPEKRPGCFL
CCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHCCCCEEEECCCCCCCEEE
ARSTPSDVARVEDRTFICSEKEEDAGPTNHWADPVEMKAKLKGLFNGCMKGRTMYVIPFC
ECCCCHHHHHHCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEEE
MGPLDSPLAKIGIEITDSPYVVTNMRIMTKMGEQVLKRLEDEVKGGGNPKRDGYGTFVPC
CCCCCCCHHHCCEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHH
LHTVGAPLADGQVDVPWPCNEEKYICHFPETEEIWSFGSGYGGNALLGKKCLALRIASAI
HHHCCCCCCCCEEECCCCCCCCCEEEECCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHH
ARKNKWMAEHMLLLGIESPDGTKAYVGGAFPSACGKTNLAMLVPPASYREAGWKTSIIGD
HHHHHHHHHEEEEEEECCCCCCEEEECCCCCCCCCCCCEEEEECCCHHHHCCCEEEECCC
DIAWIWPHEDGTFHAINPENGYFGVAPGTSYKTNPIAMDTIKENVIFTNVGLTDDGDVWW
CEEEEEECCCCCEEEECCCCCEEECCCCCCCCCCCEEHHHHCCCEEEEECCCCCCCCEEE
EGMDGPAPAHAIDWQGNDWTPDCGRKCAHPNARFTAPASQCPTIDPEWQNPEGVSLSAIL
ECCCCCCCCCEEECCCCCCCHHHHHHCCCCCCCCCCCHHHCCCCCCCCCCCCCCEEEEEE
FGGRRATTMPLVFQSRDWTHGVYVGATMGSEMTAAAFGNIGQVRRDPMAMLPFIGYHVGD
ECCCCCEEEEEEEECCCCCCCEEEEEECCCCHHHHHHCCCHHHHCCCHHHHHHHHHHHHH
YWQHWLDMGKTVANPPLIFNVNWFRKDKNGNFIWPGYGDNMRVLDWILRRAKGKAEGQKT
HHHHHHHHCHHHCCCCEEEEEEEEEECCCCCEECCCCCCCHHHHHHHHHHHCCCCCCCEE
VLGVSPTYAELDKTGLDYSEEQFNANMALNPSEWQAELESQTELFDKVGKKLPAELEEQR
EEECCCCHHHHHHCCCCCCHHHCCCCEECCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
QLLIKSFS
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA