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The map label for this gene is tuf

Identifier: 187735543

GI number: 187735543

Start: 1249991

End: 1251175

Strand: Reverse

Name: tuf

Synonym: Amuc_1048

Alternate gene names: 187735543

Gene position: 1251175-1249991 (Counterclockwise)

Preceding gene: 187735544

Following gene: 187735542

Centisome position: 46.96

GC content: 57.55

Gene sequence:

>1185_bases
ATGGCTAAAGAGCAATTCCAGCGCAACAAGCCCCACGTGAACGTGGGCACCATCGGTCACGTTGACCACGGCAAAACCTC
CCTTACCGCCGCCATTACTTCAGTGCTCGCTAAGAAGGGTTTCGCCGAAGCACGCGGCTATGACCAGATTGACGCCGCCC
CCGAAGAACGCGAACGCGGCATTACCATCTCCACGGCTCACGTTGAATACGAAACGGAAAACCGTCACTATGCCCACGTT
GACTGCCCCGGACACGCCGACTATGTGAAAAACATGATCACCGGCGCTGCCCAGATGGACGGTGCCATCCTTGTGGTTGC
CGCCTCCGACGGCCCGATGCCGCAGACCCGCGAACACATCCTCCTTGCCCGCCAGGTGGGCGTTCCCGCCATTGTCGTTT
ACATGAACAAGTGCGACCTGGTTGACGATCCCGACCTGCTTGAACTCGTGGAAATGGAAATCCGCGAACTTCTCAACGAA
TACGAATTCCCGGGCGACGACACCCCCATTATCAAGGGTTCCGCCGTTAAGGCTCTGGAAGGTGACGCCGCTGCCGAAGA
TTCCATCATGGAACTTATGGCCGCTGTTGACTCCTACATCCCGCAACCCGAACGTCCTGTGGATCAGCCCTTCCTCATGC
CTGTGGAAGACGTATTCTCCATCTCCGGCCGCGGCACCGTGGCTACCGGCCGTATCGAACGCGGCGTCATCAAGAAGATG
GAAGAAGTGGAAATCATCGGCATCAAGGACACCCAGAAAACTGCCGTTACGGACATCGAAATGTTCCGTAAGCTGCTTGA
CGAAGGCCAGGCCGGCGACAACGTTGGTCTGCTTCTCCGCGGCCTGAAGAAGGAAGACATCGAACGCGGCCAGGTAATCA
TCAAGCCCGGCACCGTGAAGCCCCACAAGAACTTCAAGGCTGAAGTTTACGTCCTGACCAAGGAAGAAGGCGGCCGTCAC
ACTCCGTTCTTCAACAACTACCGCCCGCAGTTCTACTTCCGTACAACGGACGTGACCGGTTGCTGCACCCTTCCCGAAGG
CGTGGAAATGGTGATGCCCGGCGACAACGTCAACCTGGAAGTCCAGCTCATCACTCCGATCGCCATGGAAAAAGCCATGC
GATTCGCTATCCGCGAAGGCGGCCGCACCGTGGGTGCTGGTCGTATCAGCGAAATCCTTGACTAA

Upstream 100 bases:

>100_bases
AGAGCGCATCCTTGGTAAGGATGAGGTCGCGGGTTCAATTCCCGCCAGAAGCTCCATCTTCAAGCTAGACCCATAGCAGC
CCAAACCTAAACATAATATT

Downstream 100 bases:

>100_bases
GTTCCTCAGGAACTTGTAGAGGAATCTACATCAGGTTTAATGAGGGGGCCTCCCCGCAGGCCCCCTCATTTCCCACGAAA
GAAGAGACTCCAACAGGTCA

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu

Number of amino acids: Translated: 394; Mature: 393

Protein sequence:

>394_residues
MAKEQFQRNKPHVNVGTIGHVDHGKTSLTAAITSVLAKKGFAEARGYDQIDAAPEERERGITISTAHVEYETENRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVAASDGPMPQTREHILLARQVGVPAIVVYMNKCDLVDDPDLLELVEMEIRELLNE
YEFPGDDTPIIKGSAVKALEGDAAAEDSIMELMAAVDSYIPQPERPVDQPFLMPVEDVFSISGRGTVATGRIERGVIKKM
EEVEIIGIKDTQKTAVTDIEMFRKLLDEGQAGDNVGLLLRGLKKEDIERGQVIIKPGTVKPHKNFKAEVYVLTKEEGGRH
TPFFNNYRPQFYFRTTDVTGCCTLPEGVEMVMPGDNVNLEVQLITPIAMEKAMRFAIREGGRTVGAGRISEILD

Sequences:

>Translated_394_residues
MAKEQFQRNKPHVNVGTIGHVDHGKTSLTAAITSVLAKKGFAEARGYDQIDAAPEERERGITISTAHVEYETENRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVAASDGPMPQTREHILLARQVGVPAIVVYMNKCDLVDDPDLLELVEMEIRELLNE
YEFPGDDTPIIKGSAVKALEGDAAAEDSIMELMAAVDSYIPQPERPVDQPFLMPVEDVFSISGRGTVATGRIERGVIKKM
EEVEIIGIKDTQKTAVTDIEMFRKLLDEGQAGDNVGLLLRGLKKEDIERGQVIIKPGTVKPHKNFKAEVYVLTKEEGGRH
TPFFNNYRPQFYFRTTDVTGCCTLPEGVEMVMPGDNVNLEVQLITPIAMEKAMRFAIREGGRTVGAGRISEILD
>Mature_393_residues
AKEQFQRNKPHVNVGTIGHVDHGKTSLTAAITSVLAKKGFAEARGYDQIDAAPEERERGITISTAHVEYETENRHYAHVD
CPGHADYVKNMITGAAQMDGAILVVAASDGPMPQTREHILLARQVGVPAIVVYMNKCDLVDDPDLLELVEMEIRELLNEY
EFPGDDTPIIKGSAVKALEGDAAAEDSIMELMAAVDSYIPQPERPVDQPFLMPVEDVFSISGRGTVATGRIERGVIKKME
EVEIIGIKDTQKTAVTDIEMFRKLLDEGQAGDNVGLLLRGLKKEDIERGQVIIKPGTVKPHKNFKAEVYVLTKEEGGRHT
PFFNNYRPQFYFRTTDVTGCCTLPEGVEMVMPGDNVNLEVQLITPIAMEKAMRFAIREGGRTVGAGRISEILD

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily

Homologues:

Organism=Homo sapiens, GI34147630, Length=394, Percent_Identity=56.5989847715736, Blast_Score=473, Evalue=1e-133,
Organism=Homo sapiens, GI4503471, Length=450, Percent_Identity=29.3333333333333, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI4503475, Length=450, Percent_Identity=28.8888888888889, Blast_Score=162, Evalue=4e-40,
Organism=Homo sapiens, GI223555963, Length=434, Percent_Identity=29.7235023041475, Blast_Score=159, Evalue=6e-39,
Organism=Homo sapiens, GI5729864, Length=434, Percent_Identity=29.7235023041475, Blast_Score=158, Evalue=8e-39,
Organism=Homo sapiens, GI54607086, Length=314, Percent_Identity=30.5732484076433, Blast_Score=118, Evalue=7e-27,
Organism=Homo sapiens, GI46094014, Length=328, Percent_Identity=28.9634146341463, Blast_Score=115, Evalue=5e-26,
Organism=Homo sapiens, GI194018520, Length=346, Percent_Identity=28.6127167630058, Blast_Score=115, Evalue=6e-26,
Organism=Homo sapiens, GI194097354, Length=346, Percent_Identity=28.6127167630058, Blast_Score=115, Evalue=7e-26,
Organism=Homo sapiens, GI194018522, Length=346, Percent_Identity=28.6127167630058, Blast_Score=115, Evalue=7e-26,
Organism=Homo sapiens, GI4503507, Length=259, Percent_Identity=29.3436293436293, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI157426893, Length=236, Percent_Identity=27.5423728813559, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI53729339, Length=230, Percent_Identity=32.6086956521739, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI53729337, Length=230, Percent_Identity=32.6086956521739, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI94966754, Length=145, Percent_Identity=33.1034482758621, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1790412, Length=393, Percent_Identity=74.5547073791349, Blast_Score=606, Evalue=1e-175,
Organism=Escherichia coli, GI1789737, Length=393, Percent_Identity=74.5547073791349, Blast_Score=605, Evalue=1e-174,
Organism=Escherichia coli, GI2367247, Length=376, Percent_Identity=28.7234042553192, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI48994988, Length=242, Percent_Identity=33.8842975206612, Blast_Score=104, Evalue=9e-24,
Organism=Escherichia coli, GI1789108, Length=332, Percent_Identity=30.421686746988, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1789559, Length=112, Percent_Identity=43.75, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI1788922, Length=133, Percent_Identity=33.0827067669173, Blast_Score=62, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17556456, Length=402, Percent_Identity=51.4925373134328, Blast_Score=399, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI25141371, Length=387, Percent_Identity=46.5116279069767, Blast_Score=344, Evalue=5e-95,
Organism=Caenorhabditis elegans, GI17552884, Length=460, Percent_Identity=31.5217391304348, Blast_Score=174, Evalue=8e-44,
Organism=Caenorhabditis elegans, GI17569207, Length=460, Percent_Identity=31.5217391304348, Blast_Score=174, Evalue=8e-44,
Organism=Caenorhabditis elegans, GI32566303, Length=431, Percent_Identity=30.3944315545244, Blast_Score=149, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI115532067, Length=440, Percent_Identity=28.4090909090909, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI115532065, Length=440, Percent_Identity=28.4090909090909, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI32566629, Length=359, Percent_Identity=28.133704735376, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17506081, Length=272, Percent_Identity=29.0441176470588, Blast_Score=118, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI32566301, Length=150, Percent_Identity=38, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17509919, Length=282, Percent_Identity=26.241134751773, Blast_Score=78, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17557151, Length=231, Percent_Identity=27.7056277056277, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6324761, Length=396, Percent_Identity=65.6565656565657, Blast_Score=525, Evalue=1e-150,
Organism=Saccharomyces cerevisiae, GI6325337, Length=447, Percent_Identity=30.2013422818792, Blast_Score=158, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6319594, Length=447, Percent_Identity=30.2013422818792, Blast_Score=158, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6322937, Length=455, Percent_Identity=26.1538461538462, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6320377, Length=343, Percent_Identity=26.8221574344023, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6320863, Length=263, Percent_Identity=28.5171102661597, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6323320, Length=255, Percent_Identity=31.3725490196078, Blast_Score=80, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6323098, Length=135, Percent_Identity=34.8148148148148, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI281363316, Length=393, Percent_Identity=55.9796437659033, Blast_Score=463, Evalue=1e-131,
Organism=Drosophila melanogaster, GI17864358, Length=393, Percent_Identity=55.9796437659033, Blast_Score=463, Evalue=1e-131,
Organism=Drosophila melanogaster, GI19921738, Length=388, Percent_Identity=48.7113402061856, Blast_Score=396, Evalue=1e-110,
Organism=Drosophila melanogaster, GI45553807, Length=449, Percent_Identity=28.9532293986637, Blast_Score=166, Evalue=3e-41,
Organism=Drosophila melanogaster, GI45553816, Length=449, Percent_Identity=28.9532293986637, Blast_Score=166, Evalue=3e-41,
Organism=Drosophila melanogaster, GI24651721, Length=449, Percent_Identity=28.9532293986637, Blast_Score=166, Evalue=3e-41,
Organism=Drosophila melanogaster, GI17864154, Length=449, Percent_Identity=28.9532293986637, Blast_Score=166, Evalue=3e-41,
Organism=Drosophila melanogaster, GI24652838, Length=449, Percent_Identity=29.3986636971047, Blast_Score=161, Evalue=7e-40,
Organism=Drosophila melanogaster, GI17137572, Length=449, Percent_Identity=29.3986636971047, Blast_Score=161, Evalue=7e-40,
Organism=Drosophila melanogaster, GI19922690, Length=303, Percent_Identity=32.6732673267327, Blast_Score=138, Evalue=6e-33,
Organism=Drosophila melanogaster, GI17137380, Length=350, Percent_Identity=30.2857142857143, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI45550900, Length=355, Percent_Identity=28.4507042253521, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24647046, Length=267, Percent_Identity=29.2134831460674, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24647048, Length=267, Percent_Identity=29.2134831460674, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI28572034, Length=187, Percent_Identity=36.3636363636364, Blast_Score=69, Evalue=6e-12,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): EFTU_AKKM8 (B2UQY9)

Other databases:

- EMBL:   CP001071
- RefSeq:   YP_001877655.1
- ProteinModelPortal:   B2UQY9
- SMR:   B2UQY9
- GeneID:   6274063
- GenomeReviews:   CP001071_GR
- KEGG:   amu:Amuc_1048
- HOGENOM:   HBG307581
- OMA:   RDKPFLM
- ProtClustDB:   PRK12735
- GO:   GO:0005737
- HAMAP:   MF_00118_B
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000
- PANTHER:   PTHR23115:SF31
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00485
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3; SSF50465 Elong_init_C; SSF50447 Translat_factor

EC number: 3.6.5.3

Molecular weight: Translated: 43431; Mature: 43300

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEQFQRNKPHVNVGTIGHVDHGKTSLTAAITSVLAKKGFAEARGYDQIDAAPEERERG
CCHHHHHCCCCCEEECEEECCCCCCHHHHHHHHHHHHHCCCHHHCCCCCCCCCCHHHHCC
ITISTAHVEYETENRHYAHVDCPGHADYVKNMITGAAQMDGAILVVAASDGPMPQTREHI
CEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHH
LLARQVGVPAIVVYMNKCDLVDDPDLLELVEMEIRELLNEYEFPGDDTPIIKGSAVKALE
HHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCEEECC
GDAAAEDSIMELMAAVDSYIPQPERPVDQPFLMPVEDVFSISGRGTVATGRIERGVIKKM
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCCEEEECCHHHHHHHHH
EEVEIIGIKDTQKTAVTDIEMFRKLLDEGQAGDNVGLLLRGLKKEDIERGQVIIKPGTVK
HCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCHHHHCCCEEEEECCCCC
PHKNFKAEVYVLTKEEGGRHTPFFNNYRPQFYFRTTDVTGCCTLPEGVEMVMPGDNVNLE
CCCCCCEEEEEEEECCCCCCCCCCCCCCCEEEEEECCCCEEEECCCCCEEEECCCCCEEE
VQLITPIAMEKAMRFAIREGGRTVGAGRISEILD
EEEECHHHHHHHHHHHHHHCCCEECCCHHHHHCC
>Mature Secondary Structure 
AKEQFQRNKPHVNVGTIGHVDHGKTSLTAAITSVLAKKGFAEARGYDQIDAAPEERERG
CHHHHHCCCCCEEECEEECCCCCCHHHHHHHHHHHHHCCCHHHCCCCCCCCCCHHHHCC
ITISTAHVEYETENRHYAHVDCPGHADYVKNMITGAAQMDGAILVVAASDGPMPQTREHI
CEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHH
LLARQVGVPAIVVYMNKCDLVDDPDLLELVEMEIRELLNEYEFPGDDTPIIKGSAVKALE
HHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCEEECC
GDAAAEDSIMELMAAVDSYIPQPERPVDQPFLMPVEDVFSISGRGTVATGRIERGVIKKM
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCCEEEECCHHHHHHHHH
EEVEIIGIKDTQKTAVTDIEMFRKLLDEGQAGDNVGLLLRGLKKEDIERGQVIIKPGTVK
HCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCHHHHCCCEEEEECCCCC
PHKNFKAEVYVLTKEEGGRHTPFFNNYRPQFYFRTTDVTGCCTLPEGVEMVMPGDNVNLE
CCCCCCEEEEEEEECCCCCCCCCCCCCCCEEEEEECCCCEEEECCCCCEEEECCCCCEEE
VQLITPIAMEKAMRFAIREGGRTVGAGRISEILD
EEEECHHHHHHHHHHHHHHCCCEECCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA