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The map label for this gene is dnaN [H]

Identifier: 187735318

GI number: 187735318

Start: 961035

End: 962135

Strand: Direct

Name: dnaN [H]

Synonym: Amuc_0816

Alternate gene names: 187735318

Gene position: 961035-962135 (Clockwise)

Preceding gene: 187735316

Following gene: 187735325

Centisome position: 36.07

GC content: 51.5

Gene sequence:

>1101_bases
ATGAAGTTTACCATTTCCAAGCAAGTATTTCTCGACGGGCTGAGACAGGTTGCCAGCGTTGTGTCTTCCAAGACGACGCT
GCCCATTCTTTCCAACGTCAAGATTGAAGCTGAAAACGGCCAGGTACGTTTTACGGCTACTGATCTTGATGTATGTATTA
CTGGCGTGGTTCCCGCAAATGTGCTGCGTGAAGGCACCGTGACCCTGCCCGCTAAAAAGCTGGTGAGCATTATCAGCGAA
CTTCCTGAAGCCGAAGTCCAGGTGGATGTCAACCAACGCAACCAGGCTGCTGTGGAATGTGGCCGCTCTCAGTTTAAACT
GAACGGCCTTCCGGCGGATGAATTTCCGGAACTGCCGTCTTTTGAGCAGGCTACTGTGTACCAGGTGGAGCAGAATATGT
TGCGCGACTGTATCCGCCGGACGGAATATGCCATTTCCACGGATACGACCCGCTACGTGTTGAACGGCATTTCCCTTTCT
TTCCGCAATGGTAAAATGACTTTGGTAGCCACGGACGGACGTCGTTTGGCTTTGGCGGAAACCGCCCTGGAATTTCCGGA
AGAACAGCAACTGGATGCCATCCTGCCCACTAAGGCTGTCGGAGAACTCCGCCGCCTTCTGGATGAAGTGGGAACCGTCA
CGATCCGGTTCACACGCAACCAGGCAGCCTTTGAAATCAACGATACGCTCTTAATCAGCAAACTGATTGACGGCAATTAC
CCGAACTACCGGCAGGTAATTCCTACGGATTGCAAGGAAAGTATCGAACTTCCCTGCGGGGAACTGCTGGAGACAGTGCG
CCGTGTTTCTCTCCTTTCCGTGGATAAGAGCACAAATATCAAGCTGAATTTTCGTGAAAACGAACTGGAAGTGGCTTCCA
GCGCAGAAGGAGTGGGCGAAGCTCATGAATCGATGACGATCACTTATTCCGGAAGGCCGCTTTCCATTTCCTTTAATCCG
GATTTCTTCATGGCTCCCCTGAAGACGATGGCCGGGGATACGGTCATTACGCTGAACCTCATTGATGAAATGAGTCCGGG
CGTCTTGAAGATTGGCGATGAGTTCCTGTATGTGCTCATGCCGATGAGATCTCCCAATTGA

Upstream 100 bases:

>100_bases
TTTAAAAAAATACTGCCAAATATTAGGGTTTGACGGAGAAATGGCTTGAACCATGAAAGGCACTTATGAGAAGATGCGAG
GCAGATAAAAGCACAGACAC

Downstream 100 bases:

>100_bases
TTTTGGTGGCACAAAATCGTTCATACAGAGGAACAGGCGTCATGGAGACATGACGCCTGTTTTTTTATTGGCTGAGAGTG
AATGGAAAAGAAATGTTATT

Product: DNA polymerase III, beta subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MKFTISKQVFLDGLRQVASVVSSKTTLPILSNVKIEAENGQVRFTATDLDVCITGVVPANVLREGTVTLPAKKLVSIISE
LPEAEVQVDVNQRNQAAVECGRSQFKLNGLPADEFPELPSFEQATVYQVEQNMLRDCIRRTEYAISTDTTRYVLNGISLS
FRNGKMTLVATDGRRLALAETALEFPEEQQLDAILPTKAVGELRRLLDEVGTVTIRFTRNQAAFEINDTLLISKLIDGNY
PNYRQVIPTDCKESIELPCGELLETVRRVSLLSVDKSTNIKLNFRENELEVASSAEGVGEAHESMTITYSGRPLSISFNP
DFFMAPLKTMAGDTVITLNLIDEMSPGVLKIGDEFLYVLMPMRSPN

Sequences:

>Translated_366_residues
MKFTISKQVFLDGLRQVASVVSSKTTLPILSNVKIEAENGQVRFTATDLDVCITGVVPANVLREGTVTLPAKKLVSIISE
LPEAEVQVDVNQRNQAAVECGRSQFKLNGLPADEFPELPSFEQATVYQVEQNMLRDCIRRTEYAISTDTTRYVLNGISLS
FRNGKMTLVATDGRRLALAETALEFPEEQQLDAILPTKAVGELRRLLDEVGTVTIRFTRNQAAFEINDTLLISKLIDGNY
PNYRQVIPTDCKESIELPCGELLETVRRVSLLSVDKSTNIKLNFRENELEVASSAEGVGEAHESMTITYSGRPLSISFNP
DFFMAPLKTMAGDTVITLNLIDEMSPGVLKIGDEFLYVLMPMRSPN
>Mature_366_residues
MKFTISKQVFLDGLRQVASVVSSKTTLPILSNVKIEAENGQVRFTATDLDVCITGVVPANVLREGTVTLPAKKLVSIISE
LPEAEVQVDVNQRNQAAVECGRSQFKLNGLPADEFPELPSFEQATVYQVEQNMLRDCIRRTEYAISTDTTRYVLNGISLS
FRNGKMTLVATDGRRLALAETALEFPEEQQLDAILPTKAVGELRRLLDEVGTVTIRFTRNQAAFEINDTLLISKLIDGNY
PNYRQVIPTDCKESIELPCGELLETVRRVSLLSVDKSTNIKLNFRENELEVASSAEGVGEAHESMTITYSGRPLSISFNP
DFFMAPLKTMAGDTVITLNLIDEMSPGVLKIGDEFLYVLMPMRSPN

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=373, Percent_Identity=31.9034852546917, Blast_Score=179, Evalue=4e-46,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 40545; Mature: 40545

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFTISKQVFLDGLRQVASVVSSKTTLPILSNVKIEAENGQVRFTATDLDVCITGVVPAN
CCEECHHHHHHHHHHHHHHHHHCCCCCEEEECEEEEECCCEEEEEEECEEEEEECCCCHH
VLREGTVTLPAKKLVSIISELPEAEVQVDVNQRNQAAVECGRSQFKLNGLPADEFPELPS
HHCCCCEECCHHHHHHHHHHCCCCEEEEECCCCCHHHHHCCCCEEEECCCCCCCCCCCCC
FEQATVYQVEQNMLRDCIRRTEYAISTDTTRYVLNGISLSFRNGKMTLVATDGRRLALAE
CCCHHHHHHHHHHHHHHHHHHCEEECCCCCEEEEECEEEEEECCEEEEEEECCCEEEEHH
TALEFPEEQQLDAILPTKAVGELRRLLDEVGTVTIRFTRNQAAFEINDTLLISKLIDGNY
HHHHCCCCCCCCHHCCHHHHHHHHHHHHHHCEEEEEEECCCEEEEECCCEEEEHHHCCCC
PNYRQVIPTDCKESIELPCGELLETVRRVSLLSVDKSTNIKLNFRENELEVASSAEGVGE
CCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHEEECCCCCEEEEEECCCEEHHHCCCCCCC
AHESMTITYSGRPLSISFNPDFFMAPLKTMAGDTVITLNLIDEMSPGVLKIGDEFLYVLM
CCCCEEEEECCEEEEEEECCCEEEHHHHHHCCCEEEEEEECCCCCCCEEEECCEEEEEEE
PMRSPN
ECCCCC
>Mature Secondary Structure
MKFTISKQVFLDGLRQVASVVSSKTTLPILSNVKIEAENGQVRFTATDLDVCITGVVPAN
CCEECHHHHHHHHHHHHHHHHHCCCCCEEEECEEEEECCCEEEEEEECEEEEEECCCCHH
VLREGTVTLPAKKLVSIISELPEAEVQVDVNQRNQAAVECGRSQFKLNGLPADEFPELPS
HHCCCCEECCHHHHHHHHHHCCCCEEEEECCCCCHHHHHCCCCEEEECCCCCCCCCCCCC
FEQATVYQVEQNMLRDCIRRTEYAISTDTTRYVLNGISLSFRNGKMTLVATDGRRLALAE
CCCHHHHHHHHHHHHHHHHHHCEEECCCCCEEEEECEEEEEECCEEEEEEECCCEEEEHH
TALEFPEEQQLDAILPTKAVGELRRLLDEVGTVTIRFTRNQAAFEINDTLLISKLIDGNY
HHHHCCCCCCCCHHCCHHHHHHHHHHHHHHCEEEEEEECCCEEEEECCCEEEEHHHCCCC
PNYRQVIPTDCKESIELPCGELLETVRRVSLLSVDKSTNIKLNFRENELEVASSAEGVGE
CCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHEEECCCCCEEEEEECCCEEHHHCCCCCCC
AHESMTITYSGRPLSISFNPDFFMAPLKTMAGDTVITLNLIDEMSPGVLKIGDEFLYVLM
CCCCEEEEECCEEEEEEECCCEEEHHHHHHCCCEEEEEEECCCCCCCEEEECCEEEEEEE
PMRSPN
ECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9784136 [H]