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The map label for this gene is ilvE [H]

Identifier: 187735229

GI number: 187735229

Start: 854152

End: 855018

Strand: Direct

Name: ilvE [H]

Synonym: Amuc_0723

Alternate gene names: 187735229

Gene position: 854152-855018 (Clockwise)

Preceding gene: 187735227

Following gene: 187735231

Centisome position: 32.06

GC content: 55.71

Gene sequence:

>867_bases
ATGAAGATTTGGTTAGATGGAAAGCTGGTGGAACAGGAGGAAGCAAAAACCTCGGTTTTCGACCACGGCACACTCTATGG
AGACGGCATTTTTGAAGGTATCCGGTTCTATAACAAGCGCGTTTTCCGCCTGGAAGACCACATGGACCGCCTGTACAACT
GCGCCCATTATCTGTTGCTGGATATTCCTTACACTCAGGAAGAACTCTCCAACGCCGTATGCGAAACGGTCGCGGCCTCC
GGCCTGAACGACGGCTATATCCGTCTGGTGGTTACCCGCGGCGTCGGCAATCTGGGGCTCAACCCATTCAACTGCAAACG
GTCCTGCGTCTTCATCATTGCGGATAAAATCTCCCTGTACGATCCGGACGTATATGAAAACGGGCTGGCCCTGATTACCA
GCTCCGTGCGCCGCAACCGTCCGGACACCGTTTGTCCGCAGGTCAAGTCGCTCAATTATCTCAACAACATCCTGGCCAAA
ATGGAAGCTGTGCGCCAGGGTGCGGCGGAAGCCCTGATGCTGAACGACCTGGGTAATGTGGCCGAATGCACGGGCGACAA
CATTTTCATTGTGAAGGACGGTACCGTGTTCACACCGCCAGTTACGGACGGCTGCCTGGACGGCATCACGCGCCGGGTAG
TGCTGGAAATCTGCCGGGAGCTGCAAATCCCGGCTCAGGAAAAGACCATGAACCGCTTTACCATCACCTGTGCGGACGAA
TGCTTCCTGACCGGCACTGCCGCAGAATGCGTTCCCGTGACCAAATTGGACGGCTATCAGCTGGGCTCAGGAAAAATCGG
TCCTGTCACAGCTCGAATCCTGGCCCGTTTCCAGGAACTGGCCCAGACCACGGGAACATCCTGTTAG

Upstream 100 bases:

>100_bases
ATGGAACTTTTATTTTCCTCCGGTGAATTTATAGCTTCCCCCCCTCCTCAAATAGGAGTATTCTGCTCCGGCATAAGTAA
TTTCTCAATTCTAGATAACA

Downstream 100 bases:

>100_bases
AACCAGCCAACCCTTTTTTGGACCAACGAAAACGGCTCCGCCAGATGACGGAGCCGTTTTTTTGTGCTATTCCGTTAAAT
ATAAAAAAGAAAACACAGGT

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT; Transaminase B [H]

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MKIWLDGKLVEQEEAKTSVFDHGTLYGDGIFEGIRFYNKRVFRLEDHMDRLYNCAHYLLLDIPYTQEELSNAVCETVAAS
GLNDGYIRLVVTRGVGNLGLNPFNCKRSCVFIIADKISLYDPDVYENGLALITSSVRRNRPDTVCPQVKSLNYLNNILAK
MEAVRQGAAEALMLNDLGNVAECTGDNIFIVKDGTVFTPPVTDGCLDGITRRVVLEICRELQIPAQEKTMNRFTITCADE
CFLTGTAAECVPVTKLDGYQLGSGKIGPVTARILARFQELAQTTGTSC

Sequences:

>Translated_288_residues
MKIWLDGKLVEQEEAKTSVFDHGTLYGDGIFEGIRFYNKRVFRLEDHMDRLYNCAHYLLLDIPYTQEELSNAVCETVAAS
GLNDGYIRLVVTRGVGNLGLNPFNCKRSCVFIIADKISLYDPDVYENGLALITSSVRRNRPDTVCPQVKSLNYLNNILAK
MEAVRQGAAEALMLNDLGNVAECTGDNIFIVKDGTVFTPPVTDGCLDGITRRVVLEICRELQIPAQEKTMNRFTITCADE
CFLTGTAAECVPVTKLDGYQLGSGKIGPVTARILARFQELAQTTGTSC
>Mature_288_residues
MKIWLDGKLVEQEEAKTSVFDHGTLYGDGIFEGIRFYNKRVFRLEDHMDRLYNCAHYLLLDIPYTQEELSNAVCETVAAS
GLNDGYIRLVVTRGVGNLGLNPFNCKRSCVFIIADKISLYDPDVYENGLALITSSVRRNRPDTVCPQVKSLNYLNNILAK
MEAVRQGAAEALMLNDLGNVAECTGDNIFIVKDGTVFTPPVTDGCLDGITRRVVLEICRELQIPAQEKTMNRFTITCADE
CFLTGTAAECVPVTKLDGYQLGSGKIGPVTARILARFQELAQTTGTSC

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI48994963, Length=281, Percent_Identity=37.3665480427046, Blast_Score=192, Evalue=1e-50,
Organism=Escherichia coli, GI1787338, Length=209, Percent_Identity=26.7942583732057, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005785 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 31840; Mature: 31840

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
4.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIWLDGKLVEQEEAKTSVFDHGTLYGDGIFEGIRFYNKRVFRLEDHMDRLYNCAHYLLL
CEEEECCCEECHHHHHHHHHCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
DIPYTQEELSNAVCETVAASGLNDGYIRLVVTRGVGNLGLNPFNCKRSCVFIIADKISLY
ECCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEEEECCCCCC
DPDVYENGLALITSSVRRNRPDTVCPQVKSLNYLNNILAKMEAVRQGAAEALMLNDLGNV
CCCHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
AECTGDNIFIVKDGTVFTPPVTDGCLDGITRRVVLEICRELQIPAQEKTMNRFTITCADE
HHHCCCCEEEEECCEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHCCCEEEEEECCC
CFLTGTAAECVPVTKLDGYQLGSGKIGPVTARILARFQELAQTTGTSC
EEEECCCCCEEEEECCCCEEECCCCCCHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKIWLDGKLVEQEEAKTSVFDHGTLYGDGIFEGIRFYNKRVFRLEDHMDRLYNCAHYLLL
CEEEECCCEECHHHHHHHHHCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
DIPYTQEELSNAVCETVAASGLNDGYIRLVVTRGVGNLGLNPFNCKRSCVFIIADKISLY
ECCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEEEECCCCCC
DPDVYENGLALITSSVRRNRPDTVCPQVKSLNYLNNILAKMEAVRQGAAEALMLNDLGNV
CCCHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
AECTGDNIFIVKDGTVFTPPVTDGCLDGITRRVVLEICRELQIPAQEKTMNRFTITCADE
HHHCCCCEEEEECCEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHCCCEEEEEECCC
CFLTGTAAECVPVTKLDGYQLGSGKIGPVTARILARFQELAQTTGTSC
EEEECCCCCEEEEECCCCEEECCCCCCHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]