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The map label for this gene is rho [H]

Identifier: 187735214

GI number: 187735214

Start: 834204

End: 836183

Strand: Reverse

Name: rho [H]

Synonym: Amuc_0708

Alternate gene names: 187735214

Gene position: 836183-834204 (Counterclockwise)

Preceding gene: 187735215

Following gene: 187735206

Centisome position: 31.39

GC content: 58.08

Gene sequence:

>1980_bases
ATGTCTGATACCCCCCCAGATTTGACTCCGGAACAGGTTTCTCCGGAGCCGGCGCCCAAAAAACGTATTATCCGCAAAAC
CAAGTCTGCGGCGGAAGCTCCGGTAAAGCCCCAGACTGCTGCGGAAGTGCCCGGGACGGAACAGGATTCTTCCCCGGCGC
CCCGCAAGCGGACGATAAGGAAGAAGGTTGCTGAATTGGCGGATGATGGGATGGAAAGTGTCGATGTGCCGGGGAAGAAG
ACTTCCCGTAAGAGCAGTGCTGAAATCCCGGTGGAAGAAGCGCAGGATGTTCCTGCCAGGCCCCGGCGCGGACGCCCGCG
CAAGAAGCCTGTGGAGGAGGTTCCGGAAACTGACGGCTCTGCGATTGTCTCCGGAACGGAAGCCGCCGTCAAGCCGGTGC
GCAGGCGTGCTGCCAAGGCTGTTGCTCCGGAGAACGGTGATTCAGCCGGCCCTGAGGCGGAAATTGCCGTTCATGCGGCG
CCTGCCGAGCCGGCTTCCGATTCTTCCGATTCTGTTCCGGAGCAGCCCGCAGAAGGCAGGCCGAAGGTTATTCGCCAGAG
ATTTGTGAGGAAACCGAGGGAACAGGAGCCGGAGGCGGATGCTTCCGTTCCGTCCATCAAGGTGGTGCAGGAAGGTGCGG
CGGATTTTTCCGAAGATGCCGCCCGGGAGCCGCGTCGGACAAATGAGTCGCAAAGGCAGCGTTTTGACAGGCAGAACCGC
CGGAATAACAACGACCGTTTCAACAAAAACCGGAATAACCGCCAGGATGGCCGCAATGGCAGAAACGGCAATGACCGGGT
TAAAAACCGCTGGAATAATAATCAGGAGGGCGGAACCCCACAGAACAGCGCCCCCCGTGAACTGGCGCCGCCGGAACCGG
TGGACGGGTTGCTGGAAATTACGAATAAGGGGTTCGGCTTTTTAAGGAAGCCGGATAATGATTTTGACGCTTTTGCGGAA
GCCGTTTATGTGCCGCAGGATATGATCCGCAGGTTCGGTCTGCGTCCTGCCGTGTGGGTGCATGGGCAGGCCTGCCGTCA
TGACCGCGGCATTCTTCTGACGGAAATTGCCTCCGTTAACGGGGTTGCTCCGGAGAGAGCGCGCAAGAACCCGCACTTTG
AAGAGCTCAAGGCGGTCAATCCTAATAAGCGCATTTCTTTTGAAACCCGGCCGGAGCGTTACACCACCCGCACATTGGAT
CTGATTGCTCCAATCGGGCGCGGCCAGCGCGGCCTGATTGTCTCTCCGCCCCGCGCAGGGAAGACGACGCTGCTTCAGCA
CATGGCGGAAGCCATTCTGGAGAATTACAGGGATTCCATTCACCTGATGGTGCTTCTGGTAGACGAGCGTCCGGAAGAAG
TGACGGAGTTTAAACGTTCCCTCCCCGGAGCTGAAGTATACGCTTCTTCCAATGATGGAAGGGTGCGCGATCACTGCCGC
ATGGCGGAACTTTGCATTGAACGCGCCAAACGGCTTGTAGAGGCGGGGCAGCATGTTTTCCTGCTGATGGATTCCATTAC
TCGCCTGGCCCGCGCCTACAATAACGCTGACAAGGGAAGCGGCCGCACGATGTCCGGCGGCATTGATGCCCGCGCTCTGG
AAATGCCCCGGCGCCTTTTTGCCGCCGCCCGCAATACGCGCCAGGCCGGTTCCCTGACTATCATTGCCACCGCCCTGGTG
GAAACCAACAGCCGCATGGATGACCTGATTTTCCAGGAATTCAAGGGGACGGGCAATATGGAACTTGTGCTGAACCGCCG
CATTGCGGAGCAATATATCTTCCCGGCCGTGGACATTCTGAAATCGGGGACGCGGAGGGAAGAGCTCATTATGCCGGAAG
CATGGCTGTACAAGATGAATCTGATCCGCCGCGCTTTAGCCGGGCATAAACCCGTAGAAGCCATGGAACGGTTCCTGTTT
TTCCTGAACAAATACCCCAGCAATACCCAGATGCTGCTGGATTTGAAACAGAAAGCGTAG

Upstream 100 bases:

>100_bases
CTTCTCCAATTTCGATGTGGCGGAAGAAATCCTTGAGCAGCTTGGTTAACGCTGTTGTCCTTCAGGAGACGCCTGTTGTT
GCAGTTCAATTTTCTTTTCC

Downstream 100 bases:

>100_bases
GTTTCCCGATGGCGCAGAGCCTTGGAGGCTTGCGGAATTCCGGCCGGAAAAGCGGGAAGCATTTGTCTTTCCTGATGAGT
CAGGAGCGTGCTTAAACGCC

Product: transcription termination factor Rho

Products: NA

Alternate protein names: ATP-dependent helicase Rho [H]

Number of amino acids: Translated: 659; Mature: 658

Protein sequence:

>659_residues
MSDTPPDLTPEQVSPEPAPKKRIIRKTKSAAEAPVKPQTAAEVPGTEQDSSPAPRKRTIRKKVAELADDGMESVDVPGKK
TSRKSSAEIPVEEAQDVPARPRRGRPRKKPVEEVPETDGSAIVSGTEAAVKPVRRRAAKAVAPENGDSAGPEAEIAVHAA
PAEPASDSSDSVPEQPAEGRPKVIRQRFVRKPREQEPEADASVPSIKVVQEGAADFSEDAAREPRRTNESQRQRFDRQNR
RNNNDRFNKNRNNRQDGRNGRNGNDRVKNRWNNNQEGGTPQNSAPRELAPPEPVDGLLEITNKGFGFLRKPDNDFDAFAE
AVYVPQDMIRRFGLRPAVWVHGQACRHDRGILLTEIASVNGVAPERARKNPHFEELKAVNPNKRISFETRPERYTTRTLD
LIAPIGRGQRGLIVSPPRAGKTTLLQHMAEAILENYRDSIHLMVLLVDERPEEVTEFKRSLPGAEVYASSNDGRVRDHCR
MAELCIERAKRLVEAGQHVFLLMDSITRLARAYNNADKGSGRTMSGGIDARALEMPRRLFAAARNTRQAGSLTIIATALV
ETNSRMDDLIFQEFKGTGNMELVLNRRIAEQYIFPAVDILKSGTRREELIMPEAWLYKMNLIRRALAGHKPVEAMERFLF
FLNKYPSNTQMLLDLKQKA

Sequences:

>Translated_659_residues
MSDTPPDLTPEQVSPEPAPKKRIIRKTKSAAEAPVKPQTAAEVPGTEQDSSPAPRKRTIRKKVAELADDGMESVDVPGKK
TSRKSSAEIPVEEAQDVPARPRRGRPRKKPVEEVPETDGSAIVSGTEAAVKPVRRRAAKAVAPENGDSAGPEAEIAVHAA
PAEPASDSSDSVPEQPAEGRPKVIRQRFVRKPREQEPEADASVPSIKVVQEGAADFSEDAAREPRRTNESQRQRFDRQNR
RNNNDRFNKNRNNRQDGRNGRNGNDRVKNRWNNNQEGGTPQNSAPRELAPPEPVDGLLEITNKGFGFLRKPDNDFDAFAE
AVYVPQDMIRRFGLRPAVWVHGQACRHDRGILLTEIASVNGVAPERARKNPHFEELKAVNPNKRISFETRPERYTTRTLD
LIAPIGRGQRGLIVSPPRAGKTTLLQHMAEAILENYRDSIHLMVLLVDERPEEVTEFKRSLPGAEVYASSNDGRVRDHCR
MAELCIERAKRLVEAGQHVFLLMDSITRLARAYNNADKGSGRTMSGGIDARALEMPRRLFAAARNTRQAGSLTIIATALV
ETNSRMDDLIFQEFKGTGNMELVLNRRIAEQYIFPAVDILKSGTRREELIMPEAWLYKMNLIRRALAGHKPVEAMERFLF
FLNKYPSNTQMLLDLKQKA
>Mature_658_residues
SDTPPDLTPEQVSPEPAPKKRIIRKTKSAAEAPVKPQTAAEVPGTEQDSSPAPRKRTIRKKVAELADDGMESVDVPGKKT
SRKSSAEIPVEEAQDVPARPRRGRPRKKPVEEVPETDGSAIVSGTEAAVKPVRRRAAKAVAPENGDSAGPEAEIAVHAAP
AEPASDSSDSVPEQPAEGRPKVIRQRFVRKPREQEPEADASVPSIKVVQEGAADFSEDAAREPRRTNESQRQRFDRQNRR
NNNDRFNKNRNNRQDGRNGRNGNDRVKNRWNNNQEGGTPQNSAPRELAPPEPVDGLLEITNKGFGFLRKPDNDFDAFAEA
VYVPQDMIRRFGLRPAVWVHGQACRHDRGILLTEIASVNGVAPERARKNPHFEELKAVNPNKRISFETRPERYTTRTLDL
IAPIGRGQRGLIVSPPRAGKTTLLQHMAEAILENYRDSIHLMVLLVDERPEEVTEFKRSLPGAEVYASSNDGRVRDHCRM
AELCIERAKRLVEAGQHVFLLMDSITRLARAYNNADKGSGRTMSGGIDARALEMPRRLFAAARNTRQAGSLTIIATALVE
TNSRMDDLIFQEFKGTGNMELVLNRRIAEQYIFPAVDILKSGTRREELIMPEAWLYKMNLIRRALAGHKPVEAMERFLFF
LNKYPSNTQMLLDLKQKA

Specific function: Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template [H]

COG id: COG1158

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Rho family [H]

Homologues:

Organism=Escherichia coli, GI1790217, Length=356, Percent_Identity=49.7191011235955, Blast_Score=326, Evalue=3e-90,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000194
- InterPro:   IPR003593
- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR011112
- InterPro:   IPR011113
- InterPro:   IPR004665 [H]

Pfam domain/function: PF00006 ATP-synt_ab; PF07498 Rho_N; PF07497 Rho_RNA_bind [H]

EC number: NA

Molecular weight: Translated: 73653; Mature: 73522

Theoretical pI: Translated: 10.20; Mature: 10.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDTPPDLTPEQVSPEPAPKKRIIRKTKSAAEAPVKPQTAAEVPGTEQDSSPAPRKRTIR
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
KKVAELADDGMESVDVPGKKTSRKSSAEIPVEEAQDVPARPRRGRPRKKPVEEVPETDGS
HHHHHHHHCCHHHCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCHHHCCCCCCC
AIVSGTEAAVKPVRRRAAKAVAPENGDSAGPEAEIAVHAAPAEPASDSSDSVPEQPAEGR
EEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCC
PKVIRQRFVRKPREQEPEADASVPSIKVVQEGAADFSEDAAREPRRTNESQRQRFDRQNR
HHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCCCHHHHHHCCCCCCCHHHHHHHHHHHC
RNNNDRFNKNRNNRQDGRNGRNGNDRVKNRWNNNQEGGTPQNSAPRELAPPEPVDGLLEI
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
TNKGFGFLRKPDNDFDAFAEAVYVPQDMIRRFGLRPAVWVHGQACRHDRGILLTEIASVN
HCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHCCCCCEEEECCHHHHCCCCEEEEHHHHCC
GVAPERARKNPHFEELKAVNPNKRISFETRPERYTTRTLDLIAPIGRGQRGLIVSPPRAG
CCCHHHHCCCCCHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHCCCCCCCCEEECCCCCC
KTTLLQHMAEAILENYRDSIHLMVLLVDERPEEVTEFKRSLPGAEVYASSNDGRVRDHCR
HHHHHHHHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHCCCCEEEECCCCCCHHHHHH
MAELCIERAKRLVEAGQHVFLLMDSITRLARAYNNADKGSGRTMSGGIDARALEMPRRLF
HHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHCCCCCCCCCEECCCCCHHHHHHHHHHH
AAARNTRQAGSLTIIATALVETNSRMDDLIFQEFKGTGNMELVLNRRIAEQYIFPAVDIL
HHHHCCCCCCCEEEEHHHHHHCCCHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHH
KSGTRREELIMPEAWLYKMNLIRRALAGHKPVEAMERFLFFLNKYPSNTQMLLDLKQKA
HCCCCHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCEEEHEEHHCC
>Mature Secondary Structure 
SDTPPDLTPEQVSPEPAPKKRIIRKTKSAAEAPVKPQTAAEVPGTEQDSSPAPRKRTIR
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
KKVAELADDGMESVDVPGKKTSRKSSAEIPVEEAQDVPARPRRGRPRKKPVEEVPETDGS
HHHHHHHHCCHHHCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCHHHCCCCCCC
AIVSGTEAAVKPVRRRAAKAVAPENGDSAGPEAEIAVHAAPAEPASDSSDSVPEQPAEGR
EEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCC
PKVIRQRFVRKPREQEPEADASVPSIKVVQEGAADFSEDAAREPRRTNESQRQRFDRQNR
HHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCCCHHHHHHCCCCCCCHHHHHHHHHHHC
RNNNDRFNKNRNNRQDGRNGRNGNDRVKNRWNNNQEGGTPQNSAPRELAPPEPVDGLLEI
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
TNKGFGFLRKPDNDFDAFAEAVYVPQDMIRRFGLRPAVWVHGQACRHDRGILLTEIASVN
HCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHCCCCCEEEECCHHHHCCCCEEEEHHHHCC
GVAPERARKNPHFEELKAVNPNKRISFETRPERYTTRTLDLIAPIGRGQRGLIVSPPRAG
CCCHHHHCCCCCHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHCCCCCCCCEEECCCCCC
KTTLLQHMAEAILENYRDSIHLMVLLVDERPEEVTEFKRSLPGAEVYASSNDGRVRDHCR
HHHHHHHHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHCCCCEEEECCCCCCHHHHHH
MAELCIERAKRLVEAGQHVFLLMDSITRLARAYNNADKGSGRTMSGGIDARALEMPRRLF
HHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHCCCCCCCCCEECCCCCHHHHHHHHHHH
AAARNTRQAGSLTIIATALVETNSRMDDLIFQEFKGTGNMELVLNRRIAEQYIFPAVDIL
HHHHCCCCCCCEEEEHHHHHHCCCHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHH
KSGTRREELIMPEAWLYKMNLIRRALAGHKPVEAMERFLFFLNKYPSNTQMLLDLKQKA
HCCCCHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCEEEHEEHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8051015; 10567266 [H]