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The map label for this gene is leuD [H]

Identifier: 187735173

GI number: 187735173

Start: 784866

End: 785474

Strand: Direct

Name: leuD [H]

Synonym: Amuc_0667

Alternate gene names: 187735173

Gene position: 784866-785474 (Clockwise)

Preceding gene: 187735172

Following gene: 187735174

Centisome position: 29.46

GC content: 59.61

Gene sequence:

>609_bases
ATGTCTCTGACCCAATTCACCTCCATCACCGGCACCTGCGTTCCCGTTCCGGGACCGGACATGGACACGGACCGCATCAT
CCCTGCCCGCTTCCTTAAATGCATCACGTTTGACGAACTGGCGGGAGTCATGTTCTGGGACGAACGCTTCGACGAAAACG
GACAATCCAAATCCCACCCGGTGGACGATCCCCGCTTCAAAGGGGCTTCCATCATTTTGGGCGGCTCCAACTTCGGCTGC
GGCTCCTCCCGTGAGCATGCCCCGCAGACGATCAGGCGCTCCGGCATCAAAGCCGTCATTGCGGAATCTTTCGCGGAAAT
CTTTTTCGGCAACAGCACCGGCATCGGTTTGCCCTGCGTGTGCGCGTCTGCCGCCGACATTGCCGCTCTGGCCCGGCACA
TTTCCGATCACCCGGAAACGGAAGTGACGATTGACCTCCTGAACATGACAGCTTCCTGGAAGGAAGGCTCCTTCCCCATC
CGGATGCCGGCGGAAGCCCGCGAAGCCCTGACCCGGGGCCGCTGGGATTCCATTGCGGAACTGCTTGTCAACATGGATGC
CGTAGAAAAGAAAAACAAGGAACTGCCCCAGGTCGCAGCCTCCTGCTGA

Upstream 100 bases:

>100_bases
CTGATGAGCCCGGCCATGGTGGCCGCCGCTGCTCTGACCGGGAAAGTCTCCGATGCCCGCGAAGTGTTCTCCCTGAATTA
ACTTTCCAATTTTTTCCTTT

Downstream 100 bases:

>100_bases
TTCAGCATTCACCTCTTTCCTCCCCTTCTATCCCCCTTCCCCATATGATTTTCAGAATTACAGACTACGTCCACTACGGC
ACGCTGGACAACCGTGAACG

Product: isopropylmalate isomerase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 202; Mature: 201

Protein sequence:

>202_residues
MSLTQFTSITGTCVPVPGPDMDTDRIIPARFLKCITFDELAGVMFWDERFDENGQSKSHPVDDPRFKGASIILGGSNFGC
GSSREHAPQTIRRSGIKAVIAESFAEIFFGNSTGIGLPCVCASAADIAALARHISDHPETEVTIDLLNMTASWKEGSFPI
RMPAEAREALTRGRWDSIAELLVNMDAVEKKNKELPQVAASC

Sequences:

>Translated_202_residues
MSLTQFTSITGTCVPVPGPDMDTDRIIPARFLKCITFDELAGVMFWDERFDENGQSKSHPVDDPRFKGASIILGGSNFGC
GSSREHAPQTIRRSGIKAVIAESFAEIFFGNSTGIGLPCVCASAADIAALARHISDHPETEVTIDLLNMTASWKEGSFPI
RMPAEAREALTRGRWDSIAELLVNMDAVEKKNKELPQVAASC
>Mature_201_residues
SLTQFTSITGTCVPVPGPDMDTDRIIPARFLKCITFDELAGVMFWDERFDENGQSKSHPVDDPRFKGASIILGGSNFGCG
SSREHAPQTIRRSGIKAVIAESFAEIFFGNSTGIGLPCVCASAADIAALARHISDHPETEVTIDLLNMTASWKEGSFPIR
MPAEAREALTRGRWDSIAELLVNMDAVEKKNKELPQVAASC

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786258, Length=195, Percent_Identity=36.9230769230769, Blast_Score=114, Evalue=6e-27,
Organism=Saccharomyces cerevisiae, GI6321429, Length=117, Percent_Identity=39.3162393162393, Blast_Score=91, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 21918; Mature: 21787

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLTQFTSITGTCVPVPGPDMDTDRIIPARFLKCITFDELAGVMFWDERFDENGQSKSHP
CCCCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
VDDPRFKGASIILGGSNFGCGSSREHAPQTIRRSGIKAVIAESFAEIFFGNSTGIGLPCV
CCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
CASAADIAALARHISDHPETEVTIDLLNMTASWKEGSFPIRMPAEAREALTRGRWDSIAE
HHHHHHHHHHHHHHCCCCCCEEEEEEEECEECCCCCCCCEECCHHHHHHHHCCCHHHHHH
LLVNMDAVEKKNKELPQVAASC
HHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SLTQFTSITGTCVPVPGPDMDTDRIIPARFLKCITFDELAGVMFWDERFDENGQSKSHP
CCCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
VDDPRFKGASIILGGSNFGCGSSREHAPQTIRRSGIKAVIAESFAEIFFGNSTGIGLPCV
CCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
CASAADIAALARHISDHPETEVTIDLLNMTASWKEGSFPIRMPAEAREALTRGRWDSIAE
HHHHHHHHHHHHHHCCCCCCEEEEEEEECEECCCCCCCCEECCHHHHHHHHCCCHHHHHH
LLVNMDAVEKKNKELPQVAASC
HHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA