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The map label for this gene is ddl [H]

Identifier: 187735167

GI number: 187735167

Start: 778178

End: 779113

Strand: Direct

Name: ddl [H]

Synonym: Amuc_0661

Alternate gene names: 187735167

Gene position: 778178-779113 (Clockwise)

Preceding gene: 187735166

Following gene: 187735168

Centisome position: 29.21

GC content: 59.72

Gene sequence:

>936_bases
ATGAACACTCTCAAGCGCGACCTCAAAATCGCCCTCCTGCTCGGAGGTCCCGGGGCAGAACGGGAAGTATCCCTTGTTTC
CGGCAACTCCGTTTACGACGCCCTGTGCCGGGCCGGATTCACCGATGTAACGAAGGTGGACGTGCACGGCCCGGATTTCC
GGCTGCCGGAAGGCACGGAGCTGGCCTACAACATTATCCACGGCACTTTCGGTGAGGATGGAACCCTCCAGGCCATTCTG
GAAGAACGCGGCGTTCCGTACACAGGGGCCGGCTCCCGTTCCAGCGCCCTGTGCTTCAACAAGTCAGAATCCAAAAAAGT
TTTTCTGGAAGCCGGAGTTCCTACGCCGCATGCGGAAATTCTGGACTGTTCCAATGGCATCGTGATGCCGTCCCTCCCCC
TGCCCTTCGTCATCAAGCCTCCCTGCGAGGGTTCCAGCGTGGGCGTCCACATCGTCCGCAGGCAGGAGGATGTGCTCCCC
GCCATGGAGGAAGCCGTAACCCACGGCACCACCGTATTGGTGGAAGAGTTCATTCAAGGCAAGGAACTGACGGTCGGCAT
TCTGGACGGGAAAGCCCTTCCCGTTATTCATATCTGCCCCCGTTCCGGCTTCTATGACCTGAGCAACAAATACCCGTGGA
TGAATATGGGCGGCGGCACGGATTATATTTGCCCGGCGGATCTTCCGGAAGAAACCGCCGCCAACGTGCAGGAGGCGGCC
CTGAAAGCCTACAAGGCCGCCGGCGTGGAAGTGTATGGCCGCGTGGACGTACTGCTGCGCGAGGAAGACGGCGCCCCCTT
CGTGCTGGAAATCAACACCATTCCCGGCATGACCCCTTCCAGCCTGCTTCCCAAGGCGGCGGCGGCCTCCGGATGGCCCT
ATGAAGACCTCTGCGAAAAAATAGCGGAATTATCGCTAGCCACCCCCCGTAAATAA

Upstream 100 bases:

>100_bases
AAGGAGGCCAAGGAAAACCGCGGCATCGACCTGAAATCGGAAGCCCAGGTCATCGGAGACCGGGACCCCCAGTTTTAACC
CATTCATTATTTATTCCAAT

Downstream 100 bases:

>100_bases
CGTAATATCTGACAGAGGGCGGGGAGTCGCCCCTCCCGCACCCCCCGATGTCGCACAAAGCCACAACGCCAGTCCCCCGT
TCCGCCAAAAGGCCGGAAAT

Product: D-alanine/D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase [H]

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MNTLKRDLKIALLLGGPGAEREVSLVSGNSVYDALCRAGFTDVTKVDVHGPDFRLPEGTELAYNIIHGTFGEDGTLQAIL
EERGVPYTGAGSRSSALCFNKSESKKVFLEAGVPTPHAEILDCSNGIVMPSLPLPFVIKPPCEGSSVGVHIVRRQEDVLP
AMEEAVTHGTTVLVEEFIQGKELTVGILDGKALPVIHICPRSGFYDLSNKYPWMNMGGGTDYICPADLPEETAANVQEAA
LKAYKAAGVEVYGRVDVLLREEDGAPFVLEINTIPGMTPSSLLPKAAAASGWPYEDLCEKIAELSLATPRK

Sequences:

>Translated_311_residues
MNTLKRDLKIALLLGGPGAEREVSLVSGNSVYDALCRAGFTDVTKVDVHGPDFRLPEGTELAYNIIHGTFGEDGTLQAIL
EERGVPYTGAGSRSSALCFNKSESKKVFLEAGVPTPHAEILDCSNGIVMPSLPLPFVIKPPCEGSSVGVHIVRRQEDVLP
AMEEAVTHGTTVLVEEFIQGKELTVGILDGKALPVIHICPRSGFYDLSNKYPWMNMGGGTDYICPADLPEETAANVQEAA
LKAYKAAGVEVYGRVDVLLREEDGAPFVLEINTIPGMTPSSLLPKAAAASGWPYEDLCEKIAELSLATPRK
>Mature_311_residues
MNTLKRDLKIALLLGGPGAEREVSLVSGNSVYDALCRAGFTDVTKVDVHGPDFRLPEGTELAYNIIHGTFGEDGTLQAIL
EERGVPYTGAGSRSSALCFNKSESKKVFLEAGVPTPHAEILDCSNGIVMPSLPLPFVIKPPCEGSSVGVHIVRRQEDVLP
AMEEAVTHGTTVLVEEFIQGKELTVGILDGKALPVIHICPRSGFYDLSNKYPWMNMGGGTDYICPADLPEETAANVQEAA
LKAYKAAGVEVYGRVDVLLREEDGAPFVLEINTIPGMTPSSLLPKAAAASGWPYEDLCEKIAELSLATPRK

Specific function: Cell wall formation [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786280, Length=308, Percent_Identity=40.9090909090909, Blast_Score=190, Evalue=9e-50,
Organism=Escherichia coli, GI1786579, Length=353, Percent_Identity=30.3116147308782, Blast_Score=133, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N [H]

EC number: =6.3.2.4 [H]

Molecular weight: Translated: 33295; Mature: 33295

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: PS50975 ATP_GRASP ; PS00843 DALA_DALA_LIGASE_1 ; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTLKRDLKIALLLGGPGAEREVSLVSGNSVYDALCRAGFTDVTKVDVHGPDFRLPEGTE
CCCCCCCEEEEEEECCCCCCCEEEEEECCHHHHHHHHCCCCCCEEEEECCCCCCCCCCCC
LAYNIIHGTFGEDGTLQAILEERGVPYTGAGSRSSALCFNKSESKKVFLEAGVPTPHAEI
EEEEEEECCCCCCCHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCEEEEECCCCCCCHHE
LDCSNGIVMPSLPLPFVIKPPCEGSSVGVHIVRRQEDVLPAMEEAVTHGTTVLVEEFIQG
EECCCCEEECCCCCCEEEECCCCCCCCCEEEEECCHHHHHHHHHHHHCCHHHHHHHHHCC
KELTVGILDGKALPVIHICPRSGFYDLSNKYPWMNMGGGTDYICPADLPEETAANVQEAA
CCEEEEEECCCCCCEEEECCCCCCCCCCCCCCEEECCCCCCEECCCCCCHHHHHHHHHHH
LKAYKAAGVEVYGRVDVLLREEDGAPFVLEINTIPGMTPSSLLPKAAAASGWPYEDLCEK
HHHHHHCCEEEEEEEEEEEECCCCCCEEEEECCCCCCCHHHHCCHHHHCCCCCHHHHHHH
IAELSLATPRK
HHHHCCCCCCC
>Mature Secondary Structure
MNTLKRDLKIALLLGGPGAEREVSLVSGNSVYDALCRAGFTDVTKVDVHGPDFRLPEGTE
CCCCCCCEEEEEEECCCCCCCEEEEEECCHHHHHHHHCCCCCCEEEEECCCCCCCCCCCC
LAYNIIHGTFGEDGTLQAILEERGVPYTGAGSRSSALCFNKSESKKVFLEAGVPTPHAEI
EEEEEEECCCCCCCHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCEEEEECCCCCCCHHE
LDCSNGIVMPSLPLPFVIKPPCEGSSVGVHIVRRQEDVLPAMEEAVTHGTTVLVEEFIQG
EECCCCEEECCCCCCEEEECCCCCCCCCEEEEECCHHHHHHHHHHHHCCHHHHHHHHHCC
KELTVGILDGKALPVIHICPRSGFYDLSNKYPWMNMGGGTDYICPADLPEETAANVQEAA
CCEEEEEECCCCCCEEEECCCCCCCCCCCCCCEEECCCCCCEECCCCCCHHHHHHHHHHH
LKAYKAAGVEVYGRVDVLLREEDGAPFVLEINTIPGMTPSSLLPKAAAASGWPYEDLCEK
HHHHHHCCEEEEEEEEEEEECCCCCCEEEEECCCCCCCHHHHCCHHHHCCCCCHHHHHHH
IAELSLATPRK
HHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA