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The map label for this gene is murD [H]

Identifier: 187735162

GI number: 187735162

Start: 771443

End: 772753

Strand: Direct

Name: murD [H]

Synonym: Amuc_0656

Alternate gene names: 187735162

Gene position: 771443-772753 (Clockwise)

Preceding gene: 187735161

Following gene: 187735163

Centisome position: 28.96

GC content: 60.34

Gene sequence:

>1311_bases
ATGCAGCTCAATAACCTCAACATCGCCGTTCTGGGAGCGGGAAGAAGCGGACGCGCCGCGGCGCGCCTCGCCATAAAGCA
TGGCGCACGGGTATGCGTGTTTGACGCTTCCTCCTCCATTGACGGATGGCCGGAAGACATTCCCCTGCATACCGCTGCCA
CGGAGGAAGACGGACGCGCCTTTCATGCGGATATGGTGGTCATCTCCCCCGGCATTGAGACGGATAGCCCCTTTGTCAAA
TCCTTTGGACGGGAAGGCGTGGAAACCATCGGTGAAATGGAGCTGGCATGCCGCTTTTACCATGGCCGCATCATCGCCAT
CACCGGAACCAACGGCAAAACCACCACCACATCCCTGGTGGAAAAGATTTTGCTGCGCGCCGGAAAGACCGCCGTTGCCT
GCGGCAATTACGGCGTGCCGGTAGCGGAAATCCTGCTCCGGGATTCCGTGCCGGACGTGCTGGCACTGGAGGTCAGCTCC
TTCCAGTTGGAAACCATCCGCGATTTTCACCCGGATGTGGCCGTATGGCTCAATTTTGCGCCGGACCACATGGACCGCTA
CAAATCCGTGGAGGATTACCACCGCGCCAAACTCCATATCTTCGACAACCAGACGGAAAAAGACCTGGCCGTCGTTCGCT
CTGGGGAACGGCTTCCGCACCTGAAAGCCTCCGTGCTCACCTTCAGCCCGGAAGATCCGGCGGCTGATCTTTATTACCGC
GGACCGTTGATCCTGGAGGGCGGCGCTCCCCTGCTGAACCTGGAAGGGACCACCCTGAACCAGCGCCATAATGCGGAAAA
CGCCATGGCCGCCGTCCTGGCATGCCGCCACCTGGGCATTCCCGCAGAAACAGCCGCAGAAGTATTGAAGGAGTTCGCGC
CCCCCGGCCACCGCTGCGAAACCGTCCGCACACTGGACGGCGTGCTGTGGCTGAACGATTCCAAAGCCACGAATCTGCAT
GCGCTGGAAGCGGCTCTGAAATCACAACATTCCCCCGTCATCCTGATTGCCGGAGGCAAGGATAAAGGGCTGGATTACGT
ACCCCTGCGGCCCATGCTGAAAGAAAAAGTGCGCGCCTGCGTGGTATTCGGCCAGATTGCGGACCAGCTCCAGCACGCTT
TTTCCCCTGCGGTCCCCACGGAAAAAGCCGCAGATGTAGAGGCCTGCGTGGCGAAAGCACGCTCTCTCGCCCGGCCGGGA
GACACCGTCCTGTTCTCTCCCGGCACTTCTTCCTTTGACATGTTCACCGGTTATGTTCAGCGCGGCCAGGCCTTCAGGGA
CGCGGTGAACGCCCTTCCCCCCCTTTCCTGA

Upstream 100 bases:

>100_bases
CTGGAAGGAAACGCAGGTGATTGCCCGCTTCTGGATGATCAGCCTCCTGCTGGCTTTCCTGGGCCTTTTCCTGATTACCA
CCGCCTGACAAACCACGAAC

Downstream 100 bases:

>100_bases
CAATTCACCAGAAAACGACCATCATGAAAACGACCAAGACACCTGATCACAATCCGACCGGACGCACCCGTCCGAAACGC
AAAATGGGCACCGTACTCCG

Product: UDP-N-acetylmuramoylalanine/D-glutamate ligase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 436; Mature: 436

Protein sequence:

>436_residues
MQLNNLNIAVLGAGRSGRAAARLAIKHGARVCVFDASSSIDGWPEDIPLHTAATEEDGRAFHADMVVISPGIETDSPFVK
SFGREGVETIGEMELACRFYHGRIIAITGTNGKTTTTSLVEKILLRAGKTAVACGNYGVPVAEILLRDSVPDVLALEVSS
FQLETIRDFHPDVAVWLNFAPDHMDRYKSVEDYHRAKLHIFDNQTEKDLAVVRSGERLPHLKASVLTFSPEDPAADLYYR
GPLILEGGAPLLNLEGTTLNQRHNAENAMAAVLACRHLGIPAETAAEVLKEFAPPGHRCETVRTLDGVLWLNDSKATNLH
ALEAALKSQHSPVILIAGGKDKGLDYVPLRPMLKEKVRACVVFGQIADQLQHAFSPAVPTEKAADVEACVAKARSLARPG
DTVLFSPGTSSFDMFTGYVQRGQAFRDAVNALPPLS

Sequences:

>Translated_436_residues
MQLNNLNIAVLGAGRSGRAAARLAIKHGARVCVFDASSSIDGWPEDIPLHTAATEEDGRAFHADMVVISPGIETDSPFVK
SFGREGVETIGEMELACRFYHGRIIAITGTNGKTTTTSLVEKILLRAGKTAVACGNYGVPVAEILLRDSVPDVLALEVSS
FQLETIRDFHPDVAVWLNFAPDHMDRYKSVEDYHRAKLHIFDNQTEKDLAVVRSGERLPHLKASVLTFSPEDPAADLYYR
GPLILEGGAPLLNLEGTTLNQRHNAENAMAAVLACRHLGIPAETAAEVLKEFAPPGHRCETVRTLDGVLWLNDSKATNLH
ALEAALKSQHSPVILIAGGKDKGLDYVPLRPMLKEKVRACVVFGQIADQLQHAFSPAVPTEKAADVEACVAKARSLARPG
DTVLFSPGTSSFDMFTGYVQRGQAFRDAVNALPPLS
>Mature_436_residues
MQLNNLNIAVLGAGRSGRAAARLAIKHGARVCVFDASSSIDGWPEDIPLHTAATEEDGRAFHADMVVISPGIETDSPFVK
SFGREGVETIGEMELACRFYHGRIIAITGTNGKTTTTSLVEKILLRAGKTAVACGNYGVPVAEILLRDSVPDVLALEVSS
FQLETIRDFHPDVAVWLNFAPDHMDRYKSVEDYHRAKLHIFDNQTEKDLAVVRSGERLPHLKASVLTFSPEDPAADLYYR
GPLILEGGAPLLNLEGTTLNQRHNAENAMAAVLACRHLGIPAETAAEVLKEFAPPGHRCETVRTLDGVLWLNDSKATNLH
ALEAALKSQHSPVILIAGGKDKGLDYVPLRPMLKEKVRACVVFGQIADQLQHAFSPAVPTEKAADVEACVAKARSLARPG
DTVLFSPGTSSFDMFTGYVQRGQAFRDAVNALPPLS

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=433, Percent_Identity=32.5635103926097, Blast_Score=187, Evalue=1e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 47127; Mature: 47127

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLNNLNIAVLGAGRSGRAAARLAIKHGARVCVFDASSSIDGWPEDIPLHTAATEEDGRA
CEECCEEEEEEECCCCCCHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCEECCCCCCCCE
FHADMVVISPGIETDSPFVKSFGREGVETIGEMELACRFYHGRIIAITGTNGKTTTTSLV
EEEEEEEECCCCCCCCHHHHHHCHHHHHHHHHHHHEEEEECCEEEEEECCCCCCHHHHHH
EKILLRAGKTAVACGNYGVPVAEILLRDSVPDVLALEVSSFQLETIRDFHPDVAVWLNFA
HHHHHHCCCCEEEECCCCCHHHHHHHHCCCCCEEEEEECCEEHHHHHHCCCCEEEEEECC
PDHMDRYKSVEDYHRAKLHIFDNQTEKDLAVVRSGERLPHLKASVLTFSPEDPAADLYYR
HHHHHHHHHHHHHHCEEEEEECCCCCHHHHHHHCCCCCCCCEEEEEEECCCCCCCCEEEC
GPLILEGGAPLLNLEGTTLNQRHNAENAMAAVLACRHLGIPAETAAEVLKEFAPPGHRCE
CCEEECCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHH
TVRTLDGVLWLNDSKATNLHALEAALKSQHSPVILIAGGKDKGLDYVPLRPMLKEKVRAC
HHHHHCCEEEECCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHH
VVFGQIADQLQHAFSPAVPTEKAADVEACVAKARSLARPGDTVLFSPGTSSFDMFTGYVQ
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHH
RGQAFRDAVNALPPLS
HHHHHHHHHHHCCCCC
>Mature Secondary Structure
MQLNNLNIAVLGAGRSGRAAARLAIKHGARVCVFDASSSIDGWPEDIPLHTAATEEDGRA
CEECCEEEEEEECCCCCCHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCEECCCCCCCCE
FHADMVVISPGIETDSPFVKSFGREGVETIGEMELACRFYHGRIIAITGTNGKTTTTSLV
EEEEEEEECCCCCCCCHHHHHHCHHHHHHHHHHHHEEEEECCEEEEEECCCCCCHHHHHH
EKILLRAGKTAVACGNYGVPVAEILLRDSVPDVLALEVSSFQLETIRDFHPDVAVWLNFA
HHHHHHCCCCEEEECCCCCHHHHHHHHCCCCCEEEEEECCEEHHHHHHCCCCEEEEEECC
PDHMDRYKSVEDYHRAKLHIFDNQTEKDLAVVRSGERLPHLKASVLTFSPEDPAADLYYR
HHHHHHHHHHHHHHCEEEEEECCCCCHHHHHHHCCCCCCCCEEEEEEECCCCCCCCEEEC
GPLILEGGAPLLNLEGTTLNQRHNAENAMAAVLACRHLGIPAETAAEVLKEFAPPGHRCE
CCEEECCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHH
TVRTLDGVLWLNDSKATNLHALEAALKSQHSPVILIAGGKDKGLDYVPLRPMLKEKVRAC
HHHHHCCEEEECCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHH
VVFGQIADQLQHAFSPAVPTEKAADVEACVAKARSLARPGDTVLFSPGTSSFDMFTGYVQ
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHH
RGQAFRDAVNALPPLS
HHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA