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The map label for this gene is rlmH

Identifier: 186476823

GI number: 186476823

Start: 2320535

End: 2321005

Strand: Direct

Name: rlmH

Synonym: Bphy_2069

Alternate gene names: 186476823

Gene position: 2320535-2321005 (Clockwise)

Preceding gene: 186476822

Following gene: 186476824

Centisome position: 66.7

GC content: 62.63

Gene sequence:

>471_bases
ATGAAATTGCATATCCTCGCGGTCGGGCACAAGATGCCGGACTGGATCGCCACGGGCTTCGACGAATACGCGAAGCGCAT
GCCGCCCGAGCTGCGCATCGAACTGCGCGAAATCAAGCCCGAGCAGCGCTCGTCGGGCCGCTCCGCTGAAAGCGTGATGG
CCTCCGAGCGGCAGAAGATCGAAGCAGCGCTGCCCAGAAATGCCCGCATCGTCGCACTCGACGAACGCGGCAAAGACTGG
ACGACGATGCAGCTCGCGAATGCTCTGCCAACCTGGCAACAGGACGGGCGCGACGTCGCGTTTCTGATCGGCGGCGCCGA
TGGGCTCGATCCCGACCTGAAAGCGCGCGCCGACATGTTGCTGCGCGTGTCGAGCCTCACGCTGCCGCACGCGATGGTCC
GCGTACTACTTGCTGAACAGCTGTATCGCGCATGGACCATCACGCAGAATCATCCATATCATCGAGTCTGA

Upstream 100 bases:

>100_bases
GCACGCAAGGCGCCCGCTCGCCGCCGCTCGTGAGCACGCTGTTCGAGCCGCGGTATGTGCGCGCGGCCGTGCGCGGTATG
GAATCCTCGCGAGGTCGCCG

Downstream 100 bases:

>100_bases
TCTCCGTCTGAACATACGGATATCTTCGGATTCCGCATCCCTGCGCACAACCTGGCCGGATGGCCAGGTGCGCGCCGCCC
TTCCCGTCTGTTACGCTCAA

Product: rRNA large subunit methyltransferase

Products: NA

Alternate protein names: 23S rRNA m3Psi1915 methyltransferase; rRNA (pseudouridine-N3-)-methyltransferase rlmH

Number of amino acids: Translated: 156; Mature: 156

Protein sequence:

>156_residues
MKLHILAVGHKMPDWIATGFDEYAKRMPPELRIELREIKPEQRSSGRSAESVMASERQKIEAALPRNARIVALDERGKDW
TTMQLANALPTWQQDGRDVAFLIGGADGLDPDLKARADMLLRVSSLTLPHAMVRVLLAEQLYRAWTITQNHPYHRV

Sequences:

>Translated_156_residues
MKLHILAVGHKMPDWIATGFDEYAKRMPPELRIELREIKPEQRSSGRSAESVMASERQKIEAALPRNARIVALDERGKDW
TTMQLANALPTWQQDGRDVAFLIGGADGLDPDLKARADMLLRVSSLTLPHAMVRVLLAEQLYRAWTITQNHPYHRV
>Mature_156_residues
MKLHILAVGHKMPDWIATGFDEYAKRMPPELRIELREIKPEQRSSGRSAESVMASERQKIEAALPRNARIVALDERGKDW
TTMQLANALPTWQQDGRDVAFLIGGADGLDPDLKARADMLLRVSSLTLPHAMVRVLLAEQLYRAWTITQNHPYHRV

Specific function: Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA

COG id: COG1576

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase rlmH family

Homologues:

Organism=Escherichia coli, GI1786855, Length=155, Percent_Identity=52.258064516129, Blast_Score=160, Evalue=4e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMH_BURP8 (B2JE39)

Other databases:

- EMBL:   CP001043
- RefSeq:   YP_001858293.1
- ProteinModelPortal:   B2JE39
- SMR:   B2JE39
- GeneID:   6243557
- GenomeReviews:   CP001043_GR
- KEGG:   bph:Bphy_2069
- HOGENOM:   HBG622807
- OMA:   TANHPYH
- ProtClustDB:   PRK00103
- GO:   GO:0005737
- HAMAP:   MF_00658
- InterPro:   IPR016051
- InterPro:   IPR003742
- PIRSF:   PIRSF004505
- TIGRFAMs:   TIGR00246

Pfam domain/function: PF02590 SPOUT_MTase

EC number: NA

Molecular weight: Translated: 17735; Mature: 17735

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLHILAVGHKMPDWIATGFDEYAKRMPPELRIELREIKPEQRSSGRSAESVMASERQKI
CEEEEEEECCCCCHHHHCCHHHHHHHCCHHHEEHHHHCCCHHHCCCCCHHHHHHHHHHHH
EAALPRNARIVALDERGKDWTTMQLANALPTWQQDGRDVAFLIGGADGLDPDLKARADML
HHHCCCCCEEEEECCCCCCCHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHH
LRVSSLTLPHAMVRVLLAEQLYRAWTITQNHPYHRV
HHHHHCCHHHHHHHHHHHHHHHHHEECCCCCCCCCC
>Mature Secondary Structure
MKLHILAVGHKMPDWIATGFDEYAKRMPPELRIELREIKPEQRSSGRSAESVMASERQKI
CEEEEEEECCCCCHHHHCCHHHHHHHCCHHHEEHHHHCCCHHHCCCCCHHHHHHHHHHHH
EAALPRNARIVALDERGKDWTTMQLANALPTWQQDGRDVAFLIGGADGLDPDLKARADML
HHHCCCCCEEEEECCCCCCCHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHH
LRVSSLTLPHAMVRVLLAEQLYRAWTITQNHPYHRV
HHHHHCCHHHHHHHHHHHHHHHHHEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA