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The map label for this gene is fumB [H]

Identifier: 186476696

GI number: 186476696

Start: 2186303

End: 2187826

Strand: Direct

Name: fumB [H]

Synonym: Bphy_1941

Alternate gene names: 186476696

Gene position: 2186303-2187826 (Clockwise)

Preceding gene: 186476695

Following gene: 186476697

Centisome position: 62.84

GC content: 64.24

Gene sequence:

>1524_bases
ATGACCGTCATCAAACAGGAAGACCTGATCCAGAGTATTGCGGACTCCCTGCAATACATCAGCTACTACCATCCGCTCGA
TTACATCGAGGCGCTCGGCCGCGCATACGAGCTCGAAGAAAGCCCCGCCGCCAAGGACGCCATCGCGCAGATCCTGACGA
ACAGCCGTATGTGCGCGGAAGGCAAGCGCCCGATCTGCCAGGACACGGGCATCGTCACGGTATTCGTGAAGGTCGGCATG
GACGTGCGCTGGGATGGCGCGACGATGTCGGTCACCGACATGATCAACGAAGGCGTGCGACGCGGTTATCTGAACCCGGA
CAACGTGCTGCGCGCGTCGATCGTGAGCCCGCCCGAAGGCGGCCGCAAGAACACGAAGGACAACACCCCCGCCGTGATCC
ACTACGAGATCGTGCCGGGCGACAAGGTCGACGTGCAGGTCGCGGCGAAGGGCGGCGGCTCGGAGAACAAGTCGAAGTTC
GCGATGCTGAACCCGTCGGATTCGATCGTTGACTGGATCCTGAAGACCGTGCCGACGATGGGCGCGGGCTGGTGCCCGCC
CGGCATGCTCGGCATCGGCATCGGCGGCACGGCTGAAAAGGCGATGGTCATGGCGAAGGAATCGCTGATGGACCCGATCG
ACATTCAGGACGTCATCGCTCGCGGCCCGAAGGACTGGATCGAAGAACTGCGTGTGGAACTGCACGAGAAAGTCAACGCG
CTCGGTATCGGCGCGCAGGGCTTGGGCGGTCTCGCGACGGTGCTCGACGTGAAGATCATGGCAGCGCCGACGCACGCGGC
ATCGAAGCCGGTCGCAATCATCCCGAACTGCGCGGCCACGCGCCACGCGCACTTCACGCTCGACGGCTCGGGCGTCGCGA
AGCTCACGCCGCCGCCGCTCGACGCATGGCCGAAGGTCACGTGGCAACCGAACACGGAAACCAGCAAGCGCATCGACCTG
AACACGCTGACGCCGGAAGAAGTCGCATCGTGGACGCCGGGCCAGACGCTCTTGCTGTCGGGCAAGATGCTGACGGGCCG
CGACGCCGCGCACAAGCGCATCGCCGACATGCTCGCCAAGGGCGAAAAGCTGCCCGTCGACTTCACGAACCGCGTGATCT
ATTACGTCGGCCCGGTCGACCCGGTGCGCGATGAAGCCGTCGGCCCGGCCGGCCCGACGACGGCCACGCGCATGGACAAG
TTCACGGAAACGATGCTCGCGCAAACGGGGCTGATCTCGATGATCGGCAAGGCCGAGCGCGGCCCCGTCGCGATCGAGGC
GATCAGGAAGCACAAGGCTGCGTATCTGATGGCCGTCGGCGGTGCGGCGTACCTCGTGTCGAAGGCGATCCGCAGCGCGA
AGGTGCTTGCATTCGAGGACCTCGGCATGGAAGCCATCTATGAATTCGACGTACAGGACATGCCTGTGACGGTCGCCGTC
GATTCGAACGGCACCTCGGTTCACCAGACGGGACCGAAGGAATGGCAGGCGAAGATCGGCAAGATTCCCGTCGCGACGGC
TTAA

Upstream 100 bases:

>100_bases
CGGTGCCCCTACAACGATTTACAACTCCCCTGAAGGCGCGTGAGCGGCCGCGTCGTCAAGCAGCCGCATCACACCGCCAC
CCTGCCCCACTGAGCTAGCC

Downstream 100 bases:

>100_bases
CGATTTTGTTTGTTTAGCGAAGCCGGGACGTGATAGTCCCGGCTTTTTTTATTGTTCGCGCGAAATGCAAATTAAATTGC
GCGAATGATTCTCAACAACC

Product: tartrate/fumarate subfamily Fe-S type hydro-lyase subunit alpha

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 507; Mature: 506

Protein sequence:

>507_residues
MTVIKQEDLIQSIADSLQYISYYHPLDYIEALGRAYELEESPAAKDAIAQILTNSRMCAEGKRPICQDTGIVTVFVKVGM
DVRWDGATMSVTDMINEGVRRGYLNPDNVLRASIVSPPEGGRKNTKDNTPAVIHYEIVPGDKVDVQVAAKGGGSENKSKF
AMLNPSDSIVDWILKTVPTMGAGWCPPGMLGIGIGGTAEKAMVMAKESLMDPIDIQDVIARGPKDWIEELRVELHEKVNA
LGIGAQGLGGLATVLDVKIMAAPTHAASKPVAIIPNCAATRHAHFTLDGSGVAKLTPPPLDAWPKVTWQPNTETSKRIDL
NTLTPEEVASWTPGQTLLLSGKMLTGRDAAHKRIADMLAKGEKLPVDFTNRVIYYVGPVDPVRDEAVGPAGPTTATRMDK
FTETMLAQTGLISMIGKAERGPVAIEAIRKHKAAYLMAVGGAAYLVSKAIRSAKVLAFEDLGMEAIYEFDVQDMPVTVAV
DSNGTSVHQTGPKEWQAKIGKIPVATA

Sequences:

>Translated_507_residues
MTVIKQEDLIQSIADSLQYISYYHPLDYIEALGRAYELEESPAAKDAIAQILTNSRMCAEGKRPICQDTGIVTVFVKVGM
DVRWDGATMSVTDMINEGVRRGYLNPDNVLRASIVSPPEGGRKNTKDNTPAVIHYEIVPGDKVDVQVAAKGGGSENKSKF
AMLNPSDSIVDWILKTVPTMGAGWCPPGMLGIGIGGTAEKAMVMAKESLMDPIDIQDVIARGPKDWIEELRVELHEKVNA
LGIGAQGLGGLATVLDVKIMAAPTHAASKPVAIIPNCAATRHAHFTLDGSGVAKLTPPPLDAWPKVTWQPNTETSKRIDL
NTLTPEEVASWTPGQTLLLSGKMLTGRDAAHKRIADMLAKGEKLPVDFTNRVIYYVGPVDPVRDEAVGPAGPTTATRMDK
FTETMLAQTGLISMIGKAERGPVAIEAIRKHKAAYLMAVGGAAYLVSKAIRSAKVLAFEDLGMEAIYEFDVQDMPVTVAV
DSNGTSVHQTGPKEWQAKIGKIPVATA
>Mature_506_residues
TVIKQEDLIQSIADSLQYISYYHPLDYIEALGRAYELEESPAAKDAIAQILTNSRMCAEGKRPICQDTGIVTVFVKVGMD
VRWDGATMSVTDMINEGVRRGYLNPDNVLRASIVSPPEGGRKNTKDNTPAVIHYEIVPGDKVDVQVAAKGGGSENKSKFA
MLNPSDSIVDWILKTVPTMGAGWCPPGMLGIGIGGTAEKAMVMAKESLMDPIDIQDVIARGPKDWIEELRVELHEKVNAL
GIGAQGLGGLATVLDVKIMAAPTHAASKPVAIIPNCAATRHAHFTLDGSGVAKLTPPPLDAWPKVTWQPNTETSKRIDLN
TLTPEEVASWTPGQTLLLSGKMLTGRDAAHKRIADMLAKGEKLPVDFTNRVIYYVGPVDPVRDEAVGPAGPTTATRMDKF
TETMLAQTGLISMIGKAERGPVAIEAIRKHKAAYLMAVGGAAYLVSKAIRSAKVLAFEDLGMEAIYEFDVQDMPVTVAVD
SNGTSVHQTGPKEWQAKIGKIPVATA

Specific function: It functions in the generation of fumarate for use as an anaerobic electron acceptor [H]

COG id: COG1951

COG function: function code C; Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1787897, Length=468, Percent_Identity=32.2649572649573, Blast_Score=205, Evalue=7e-54,
Organism=Escherichia coli, GI1790564, Length=467, Percent_Identity=31.0492505353319, Blast_Score=204, Evalue=1e-53,
Organism=Escherichia coli, GI1789442, Length=291, Percent_Identity=29.553264604811, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1789443, Length=178, Percent_Identity=26.9662921348315, Blast_Score=83, Evalue=4e-17,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 54415; Mature: 54284

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVIKQEDLIQSIADSLQYISYYHPLDYIEALGRAYELEESPAAKDAIAQILTNSRMCAE
CCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHEECCCCCCHHHHHHHHHHCCCHHHC
GKRPICQDTGIVTVFVKVGMDVRWDGATMSVTDMINEGVRRGYLNPDNVLRASIVSPPEG
CCCCCCCCCCEEEEEEEECCEEEECCCCCHHHHHHHHHHHCCCCCHHHHEEEEECCCCCC
GRKNTKDNTPAVIHYEIVPGDKVDVQVAAKGGGSENKSKFAMLNPSDSIVDWILKTVPTM
CCCCCCCCCCEEEEEEECCCCCEEEEEEECCCCCCCCCEEEEECCCHHHHHHHHHHHCCC
GAGWCPPGMLGIGIGGTAEKAMVMAKESLMDPIDIQDVIARGPKDWIEELRVELHEKVNA
CCCCCCCCCEEECCCCCHHHHHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHH
LGIGAQGLGGLATVLDVKIMAAPTHAASKPVAIIPNCAATRHAHFTLDGSGVAKLTPPPL
EECCCCCCCHHHHHHEEEEEECCCCCCCCCEEEECCCCCCCCEEEEECCCCEEECCCCCC
DAWPKVTWQPNTETSKRIDLNTLTPEEVASWTPGQTLLLSGKMLTGRDAAHKRIADMLAK
CCCCCCEECCCCCCCCEEEECCCCHHHHHCCCCCCEEEEECCEECCCHHHHHHHHHHHHC
GEKLPVDFTNRVIYYVGPVDPVRDEAVGPAGPTTATRMDKFTETMLAQTGLISMIGKAER
CCCCCEEECCCEEEEECCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
GPVAIEAIRKHKAAYLMAVGGAAYLVSKAIRSAKVLAFEDLGMEAIYEFDVQDMPVTVAV
CCHHHHHHHHHCCEEEEEECHHHHHHHHHHHHHEEEEEHHCCCCEEEECCCCCCCEEEEE
DSNGTSVHQTGPKEWQAKIGKIPVATA
CCCCCEECCCCCHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TVIKQEDLIQSIADSLQYISYYHPLDYIEALGRAYELEESPAAKDAIAQILTNSRMCAE
CCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHEECCCCCCHHHHHHHHHHCCCHHHC
GKRPICQDTGIVTVFVKVGMDVRWDGATMSVTDMINEGVRRGYLNPDNVLRASIVSPPEG
CCCCCCCCCCEEEEEEEECCEEEECCCCCHHHHHHHHHHHCCCCCHHHHEEEEECCCCCC
GRKNTKDNTPAVIHYEIVPGDKVDVQVAAKGGGSENKSKFAMLNPSDSIVDWILKTVPTM
CCCCCCCCCCEEEEEEECCCCCEEEEEEECCCCCCCCCEEEEECCCHHHHHHHHHHHCCC
GAGWCPPGMLGIGIGGTAEKAMVMAKESLMDPIDIQDVIARGPKDWIEELRVELHEKVNA
CCCCCCCCCEEECCCCCHHHHHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHH
LGIGAQGLGGLATVLDVKIMAAPTHAASKPVAIIPNCAATRHAHFTLDGSGVAKLTPPPL
EECCCCCCCHHHHHHEEEEEECCCCCCCCCEEEECCCCCCCCEEEEECCCCEEECCCCCC
DAWPKVTWQPNTETSKRIDLNTLTPEEVASWTPGQTLLLSGKMLTGRDAAHKRIADMLAK
CCCCCCEECCCCCCCCEEEECCCCHHHHHCCCCCCEEEEECCEECCCHHHHHHHHHHHHC
GEKLPVDFTNRVIYYVGPVDPVRDEAVGPAGPTTATRMDKFTETMLAQTGLISMIGKAER
CCCCCEEECCCEEEEECCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
GPVAIEAIRKHKAAYLMAVGGAAYLVSKAIRSAKVLAFEDLGMEAIYEFDVQDMPVTVAV
CCHHHHHHHHHCCEEEEEECHHHHHHHHHHHHHEEEEEHHCCCCEEEECCCCCCCEEEEE
DSNGTSVHQTGPKEWQAKIGKIPVATA
CCCCCEECCCCCHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2656658; 7610040; 9278503 [H]