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The map label for this gene is ghrB [H]

Identifier: 186476010

GI number: 186476010

Start: 1401192

End: 1402160

Strand: Reverse

Name: ghrB [H]

Synonym: Bphy_1251

Alternate gene names: 186476010

Gene position: 1402160-1401192 (Counterclockwise)

Preceding gene: 186476011

Following gene: 186476009

Centisome position: 40.3

GC content: 65.74

Gene sequence:

>969_bases
ATGAAGCGAAAGATCGTCGCATACAAGCCGCTGCCGGATGACGTGCTCTCGTATCTTAAGCAGCATGCCGACGTCGTGCA
GGCGGACGCCGCGCAGCACGACGCGTTCGTCGCCGCGCTGCACGACGCCGAAGGCGCGATCGGCGCGAGCGTGAAGATCA
CGCCGGCGATGCTCGACGGCGCAGCGAAGCTCAAGGCGCTCTCGACGATCTCGGTCGGCTACGACAACTTCGACGTCGCC
GATCTGACGAAGCGCGGCATCGTGCTCGCGCATACGCCCGACGTGCTGACGGAATCCACGGCGGACACGGTGTTCGCGCT
GATTCTGTCGAGCGCGCGGCGCGTCGTCGAACTCGCGGACTGGGTGAAGGCGGGCGAGTGGAAGGCGAGCATCGGGCCAG
CGCTGTATGGCGTCGACGTGCAGGGCAAGACGCTCGGCATCGTCGGTCTGGGACGCATCGGCGGCGCGGTGGCACGCCGT
GCGGCGCTCGGCTTCAACATGAAGGTTCTCTACACGAACCGCAGCGCGAACGCGCAAGCGGAACAGCGCTATGGCGCACG
CCGTGTCGAACTCGACGAACTGCTCACGACATCCGATTTCGTGTGCCTGCAAGTGCCGCTGTCGCCGCAAACGCGGCACA
TGATCGGCGCGAACGAACTGCGCAAGATGAAGAAAAGCGCGATCCTGATCAACGCGTCGCGCGGCCAGACCGTCGACGAG
CACGCGCTGATCGCCGCCTTGCAGGCGGGTACGATCCATGGCGCGGGCCTCGACGTATACGACAAGGAGCCGCTCGATCC
GGCATCGCCGCTGCTGAAGATGAGCAACGTGGTGGCGTTGCCGCACATCGGCTCCGCGACGCACGAGACGCGCCACGCGA
TGGCGCGTTGCGCGGCGGAGAATCTGGTCGGCGCACTCGACGGCACGCTGAAGATCAACATCGTCAACCGCGACGTCCTT
TCGCCATGA

Upstream 100 bases:

>100_bases
TTCATGAGCGTCGCGTTGCTTGCCGCCGTAGTCCTGACGCTTGCCGTCAAGCCGCAGCCGATGCAGACTCCGACACTCGC
CACTCAAGGAAAGTGAATCG

Downstream 100 bases:

>100_bases
GTACGCCGGGTTCAGGCGCACCGCGCCGCGCCACGATCAGCGACGTCGCGCGCGAGGCGGGCACGGGCAAGACGAGCATC
TCGCGCTATCTGAACGGGGA

Product: D-isomer specific 2-hydroxyacid dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MKRKIVAYKPLPDDVLSYLKQHADVVQADAAQHDAFVAALHDAEGAIGASVKITPAMLDGAAKLKALSTISVGYDNFDVA
DLTKRGIVLAHTPDVLTESTADTVFALILSSARRVVELADWVKAGEWKASIGPALYGVDVQGKTLGIVGLGRIGGAVARR
AALGFNMKVLYTNRSANAQAEQRYGARRVELDELLTTSDFVCLQVPLSPQTRHMIGANELRKMKKSAILINASRGQTVDE
HALIAALQAGTIHGAGLDVYDKEPLDPASPLLKMSNVVALPHIGSATHETRHAMARCAAENLVGALDGTLKINIVNRDVL
SP

Sequences:

>Translated_322_residues
MKRKIVAYKPLPDDVLSYLKQHADVVQADAAQHDAFVAALHDAEGAIGASVKITPAMLDGAAKLKALSTISVGYDNFDVA
DLTKRGIVLAHTPDVLTESTADTVFALILSSARRVVELADWVKAGEWKASIGPALYGVDVQGKTLGIVGLGRIGGAVARR
AALGFNMKVLYTNRSANAQAEQRYGARRVELDELLTTSDFVCLQVPLSPQTRHMIGANELRKMKKSAILINASRGQTVDE
HALIAALQAGTIHGAGLDVYDKEPLDPASPLLKMSNVVALPHIGSATHETRHAMARCAAENLVGALDGTLKINIVNRDVL
SP
>Mature_322_residues
MKRKIVAYKPLPDDVLSYLKQHADVVQADAAQHDAFVAALHDAEGAIGASVKITPAMLDGAAKLKALSTISVGYDNFDVA
DLTKRGIVLAHTPDVLTESTADTVFALILSSARRVVELADWVKAGEWKASIGPALYGVDVQGKTLGIVGLGRIGGAVARR
AALGFNMKVLYTNRSANAQAEQRYGARRVELDELLTTSDFVCLQVPLSPQTRHMIGANELRKMKKSAILINASRGQTVDE
HALIAALQAGTIHGAGLDVYDKEPLDPASPLLKMSNVVALPHIGSATHETRHAMARCAAENLVGALDGTLKINIVNRDVL
SP

Specific function: Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively [H]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GhrB subfamily [H]

Homologues:

Organism=Homo sapiens, GI6912396, Length=253, Percent_Identity=42.6877470355731, Blast_Score=194, Evalue=8e-50,
Organism=Homo sapiens, GI23308577, Length=303, Percent_Identity=32.6732673267327, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI4557497, Length=319, Percent_Identity=33.8557993730408, Blast_Score=137, Evalue=9e-33,
Organism=Homo sapiens, GI61743967, Length=319, Percent_Identity=33.8557993730408, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI145580578, Length=316, Percent_Identity=31.6455696202532, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI4557499, Length=316, Percent_Identity=31.6455696202532, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI145580575, Length=316, Percent_Identity=31.6455696202532, Blast_Score=129, Evalue=3e-30,
Organism=Escherichia coli, GI87082289, Length=324, Percent_Identity=57.0987654320988, Blast_Score=366, Evalue=1e-102,
Organism=Escherichia coli, GI1789279, Length=272, Percent_Identity=34.1911764705882, Blast_Score=118, Evalue=5e-28,
Organism=Escherichia coli, GI1787645, Length=200, Percent_Identity=28, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI87081824, Length=145, Percent_Identity=33.7931034482759, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17532191, Length=304, Percent_Identity=32.5657894736842, Blast_Score=138, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI25147481, Length=268, Percent_Identity=31.7164179104478, Blast_Score=119, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6324055, Length=240, Percent_Identity=38.75, Blast_Score=162, Evalue=8e-41,
Organism=Saccharomyces cerevisiae, GI6320925, Length=262, Percent_Identity=35.1145038167939, Blast_Score=129, Evalue=9e-31,
Organism=Saccharomyces cerevisiae, GI6322116, Length=276, Percent_Identity=32.9710144927536, Blast_Score=124, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6321253, Length=258, Percent_Identity=29.0697674418605, Blast_Score=82, Evalue=8e-17,
Organism=Saccharomyces cerevisiae, GI6324964, Length=241, Percent_Identity=30.2904564315353, Blast_Score=79, Evalue=9e-16,
Organism=Saccharomyces cerevisiae, GI6325144, Length=178, Percent_Identity=31.4606741573034, Blast_Score=78, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6324980, Length=180, Percent_Identity=30.5555555555556, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI45551003, Length=310, Percent_Identity=36.7741935483871, Blast_Score=194, Evalue=6e-50,
Organism=Drosophila melanogaster, GI45552429, Length=310, Percent_Identity=36.7741935483871, Blast_Score=194, Evalue=7e-50,
Organism=Drosophila melanogaster, GI24585514, Length=310, Percent_Identity=36.7741935483871, Blast_Score=194, Evalue=7e-50,
Organism=Drosophila melanogaster, GI28574282, Length=310, Percent_Identity=36.7741935483871, Blast_Score=194, Evalue=7e-50,
Organism=Drosophila melanogaster, GI28574284, Length=310, Percent_Identity=36.7741935483871, Blast_Score=194, Evalue=8e-50,
Organism=Drosophila melanogaster, GI28571528, Length=279, Percent_Identity=41.9354838709677, Blast_Score=189, Evalue=2e-48,
Organism=Drosophila melanogaster, GI28574286, Length=311, Percent_Identity=36.0128617363344, Blast_Score=184, Evalue=1e-46,
Organism=Drosophila melanogaster, GI24585516, Length=253, Percent_Identity=33.9920948616601, Blast_Score=154, Evalue=7e-38,
Organism=Drosophila melanogaster, GI19921140, Length=263, Percent_Identity=35.361216730038, Blast_Score=135, Evalue=4e-32,
Organism=Drosophila melanogaster, GI24646446, Length=321, Percent_Identity=34.2679127725857, Blast_Score=127, Evalue=9e-30,
Organism=Drosophila melanogaster, GI24646448, Length=321, Percent_Identity=34.2679127725857, Blast_Score=127, Evalue=9e-30,
Organism=Drosophila melanogaster, GI24646452, Length=321, Percent_Identity=34.2679127725857, Blast_Score=127, Evalue=9e-30,
Organism=Drosophila melanogaster, GI24646450, Length=321, Percent_Identity=34.2679127725857, Blast_Score=127, Evalue=9e-30,
Organism=Drosophila melanogaster, GI62472511, Length=321, Percent_Identity=34.2679127725857, Blast_Score=127, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.79; =1.1.1.81 [H]

Molecular weight: Translated: 34249; Mature: 34249

Theoretical pI: Translated: 8.25; Mature: 8.25

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRKIVAYKPLPDDVLSYLKQHADVVQADAAQHDAFVAALHDAEGAIGASVKITPAMLDG
CCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCEEEEEHHHHCC
AAKLKALSTISVGYDNFDVADLTKRGIVLAHTPDVLTESTADTVFALILSSARRVVELAD
HHHHHHEEEEEECCCCCCHHHHHHCCEEEEECCCHHHCCHHHHHHHHHHHHHHHHHHHHH
WVKAGEWKASIGPALYGVDVQGKTLGIVGLGRIGGAVARRAALGFNMKVLYTNRSANAQA
HHHCCCCCCCCCCEEEEEECCCCEEEEEEEHHHHHHHHHHHHHCCCEEEEEECCCCCCHH
EQRYGARRVELDELLTTSDFVCLQVPLSPQTRHMIGANELRKMKKSAILINASRGQTVDE
HHHCCCCCEEHHHHHCCCCEEEEEECCCCCCHHHCCHHHHHHHHHCEEEEECCCCCCCHH
HALIAALQAGTIHGAGLDVYDKEPLDPASPLLKMSNVVALPHIGSATHETRHAMARCAAE
HHHHHHHHCCCEECCCCCCCCCCCCCHHHHHHHHHCEEECCCCCCCHHHHHHHHHHHHHH
NLVGALDGTLKINIVNRDVLSP
HHHHHCCCEEEEEEECCCCCCC
>Mature Secondary Structure
MKRKIVAYKPLPDDVLSYLKQHADVVQADAAQHDAFVAALHDAEGAIGASVKITPAMLDG
CCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCEEEEEHHHHCC
AAKLKALSTISVGYDNFDVADLTKRGIVLAHTPDVLTESTADTVFALILSSARRVVELAD
HHHHHHEEEEEECCCCCCHHHHHHCCEEEEECCCHHHCCHHHHHHHHHHHHHHHHHHHHH
WVKAGEWKASIGPALYGVDVQGKTLGIVGLGRIGGAVARRAALGFNMKVLYTNRSANAQA
HHHCCCCCCCCCCEEEEEECCCCEEEEEEEHHHHHHHHHHHHHCCCEEEEEECCCCCCHH
EQRYGARRVELDELLTTSDFVCLQVPLSPQTRHMIGANELRKMKKSAILINASRGQTVDE
HHHCCCCCEEHHHHHCCCCEEEEEECCCCCCHHHCCHHHHHHHHHCEEEEECCCCCCCHH
HALIAALQAGTIHGAGLDVYDKEPLDPASPLLKMSNVVALPHIGSATHETRHAMARCAAE
HHHHHHHHCCCEECCCCCCCCCCCCCHHHHHHHHHCEEECCCCCCCHHHHHHHHHHHHHH
NLVGALDGTLKINIVNRDVLSP
HHHHHCCCEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA