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The map label for this gene is gatB

Identifier: 183222289

GI number: 183222289

Start: 3039575

End: 3041038

Strand: Reverse

Name: gatB

Synonym: LEPBI_I2941

Alternate gene names: 183222289

Gene position: 3041038-3039575 (Counterclockwise)

Preceding gene: 183222290

Following gene: 183222285

Centisome position: 84.48

GC content: 39.48

Gene sequence:

>1464_bases
ATGGAATACGAAGTCATCATCGGTCTGGAAGTCCACGTCCAGCTCAATACCAATTCAAAAATATTTTCCACTGCCACAAA
CGAATTTGGTGGTAGTCCCAATACTCATATTTCTCCTTTATGTGTTGCCTTGCCTGGCACCTTGCCAGTGTTAAACGAGG
TGGTCCTTGAAAAAGCAGTCCGAGCTGGTGTGGCTCTTGGTTGTGAGATCACAAAATTTACAAAATTTGATCGTAAAAAT
TATTTTTATCCAGACTTACCGAAAGGGTATCAAATTTCACAATTTGATAAACCATATGCTACGAAAGGTGGAATTCATGT
TCTATTAAAAGGGGAAAAAGAAGAAAAGTTCATTCCTCTGACTCGTATCCACATGGAAGAAGATGCGGGTAAACTCATCC
ACTCTCACGATCCTTCTATCAATCGTTCGTATGTGGATTATAACCGTGCAGGGACACCTCTCATCGAAATTGTCTCCGAA
CCAGACCTAAGATCTTCTGATGAGGCTTATGTATATTTAAATGAATTAAAAACCATCCTTCGTTACATCCAAGTATCTGA
TTGTAATATGGAAGAGGGATCACTACGATGTGATGCAAACGTCTCCATTCGTCCGAAAGGAGAAAAAGGATTTCGTACTC
GAGTGGAAATCAAAAACCTAAACTCATTTAAGGCCGTAAAACAAGCGATAGACTATGAAGTCGAATGGCAAAAAGATGTA
TATTCTAGAGGTGAGTCCTTCAAACAAATGACAAAACTTTGGGATGCAACACTACTCAAAACCATCCCGATGCGTTCCAA
AGAGATGAGCCATGACTATCGTTATTTTCCAGAACCAGATTTACCTACGATTCAAATTTCGGATTCTTTCATTGAAGACA
TTCGTAAAACTCTTCCTGAGTTGCCACGACAAAAAAAAGAACGTTACAAAACAGAACTTGGACTTCCCGAATATGATGCG
GAAGTTCTCACTAGTGAACGTGAAATCGCAGAATACTTTGAAGAGGCACTTGTCATTGCAGGTGATGCAAAAAAAACTTC
AAATTGGGTCAAAGATGAAATCTTAGGAATTGTGAATAAAGAGAATATTTCCATCCAAGAGTTTGCCATTGATCCAGTGC
GGATTGGTAAACTTGTGAAACTCATCAACTCTGGTGAAATCACAGGAAAAATTGCGAAGACGATTTTTGAAGATATGTTA
ACTACAAAAGACCAACCCGAAACCATTGTGGAGAAAAATGGTCTGAAAGTGGTTCGTGATGACAAAGCCCTTGAAGAAAT
TGTGATCCGTGTGATTGAATCCCAACCAGAATCTGTGGAAGGTTGGAAAAATGGAAAAGACCGAGTGCTTGGTGCCATTG
TCGGTGGAGTCATGAAAGAAACAAAGGGGAAAGCTGATCCAAAACTTGTAAATGAACTGATACTCGCCAAATTAGGCCCG
TTAGGTGAAAAAAAGAAGGTATAA

Upstream 100 bases:

>100_bases
TATAAATTGGTCCTACAATCGGTCCCAGACCTCTTGGATTAAAAACTGGAGGAATTTGAATTGCCAGGTTCATTTTCCGT
GAAAATCCTGTTACCAAACC

Downstream 100 bases:

>100_bases
GCCTTTAGATTCGTTTAGGAAACAGTTGTCTGTTAAGTGATTTCTAGTTTGTGAACAGAACCAAAATCCACAGGGGACTT
AAATGCTTCCTCATGTGGAA

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B

Number of amino acids: Translated: 487; Mature: 487

Protein sequence:

>487_residues
MEYEVIIGLEVHVQLNTNSKIFSTATNEFGGSPNTHISPLCVALPGTLPVLNEVVLEKAVRAGVALGCEITKFTKFDRKN
YFYPDLPKGYQISQFDKPYATKGGIHVLLKGEKEEKFIPLTRIHMEEDAGKLIHSHDPSINRSYVDYNRAGTPLIEIVSE
PDLRSSDEAYVYLNELKTILRYIQVSDCNMEEGSLRCDANVSIRPKGEKGFRTRVEIKNLNSFKAVKQAIDYEVEWQKDV
YSRGESFKQMTKLWDATLLKTIPMRSKEMSHDYRYFPEPDLPTIQISDSFIEDIRKTLPELPRQKKERYKTELGLPEYDA
EVLTSEREIAEYFEEALVIAGDAKKTSNWVKDEILGIVNKENISIQEFAIDPVRIGKLVKLINSGEITGKIAKTIFEDML
TTKDQPETIVEKNGLKVVRDDKALEEIVIRVIESQPESVEGWKNGKDRVLGAIVGGVMKETKGKADPKLVNELILAKLGP
LGEKKKV

Sequences:

>Translated_487_residues
MEYEVIIGLEVHVQLNTNSKIFSTATNEFGGSPNTHISPLCVALPGTLPVLNEVVLEKAVRAGVALGCEITKFTKFDRKN
YFYPDLPKGYQISQFDKPYATKGGIHVLLKGEKEEKFIPLTRIHMEEDAGKLIHSHDPSINRSYVDYNRAGTPLIEIVSE
PDLRSSDEAYVYLNELKTILRYIQVSDCNMEEGSLRCDANVSIRPKGEKGFRTRVEIKNLNSFKAVKQAIDYEVEWQKDV
YSRGESFKQMTKLWDATLLKTIPMRSKEMSHDYRYFPEPDLPTIQISDSFIEDIRKTLPELPRQKKERYKTELGLPEYDA
EVLTSEREIAEYFEEALVIAGDAKKTSNWVKDEILGIVNKENISIQEFAIDPVRIGKLVKLINSGEITGKIAKTIFEDML
TTKDQPETIVEKNGLKVVRDDKALEEIVIRVIESQPESVEGWKNGKDRVLGAIVGGVMKETKGKADPKLVNELILAKLGP
LGEKKKV
>Mature_487_residues
MEYEVIIGLEVHVQLNTNSKIFSTATNEFGGSPNTHISPLCVALPGTLPVLNEVVLEKAVRAGVALGCEITKFTKFDRKN
YFYPDLPKGYQISQFDKPYATKGGIHVLLKGEKEEKFIPLTRIHMEEDAGKLIHSHDPSINRSYVDYNRAGTPLIEIVSE
PDLRSSDEAYVYLNELKTILRYIQVSDCNMEEGSLRCDANVSIRPKGEKGFRTRVEIKNLNSFKAVKQAIDYEVEWQKDV
YSRGESFKQMTKLWDATLLKTIPMRSKEMSHDYRYFPEPDLPTIQISDSFIEDIRKTLPELPRQKKERYKTELGLPEYDA
EVLTSEREIAEYFEEALVIAGDAKKTSNWVKDEILGIVNKENISIQEFAIDPVRIGKLVKLINSGEITGKIAKTIFEDML
TTKDQPETIVEKNGLKVVRDDKALEEIVIRVIESQPESVEGWKNGKDRVLGAIVGGVMKETKGKADPKLVNELILAKLGP
LGEKKKV

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily

Homologues:

Organism=Homo sapiens, GI4758894, Length=497, Percent_Identity=36.2173038229376, Blast_Score=315, Evalue=6e-86,
Organism=Caenorhabditis elegans, GI115534094, Length=342, Percent_Identity=33.9181286549708, Blast_Score=199, Evalue=2e-51,
Organism=Saccharomyces cerevisiae, GI6319391, Length=511, Percent_Identity=30.3326810176125, Blast_Score=220, Evalue=4e-58,
Organism=Drosophila melanogaster, GI45550808, Length=486, Percent_Identity=33.7448559670782, Blast_Score=277, Evalue=1e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATB_LEPBA (B0SFE8)

Other databases:

- EMBL:   CP000777
- RefSeq:   YP_001963894.1
- ProteinModelPortal:   B0SFE8
- SMR:   B0SFE8
- GeneID:   6388943
- GenomeReviews:   CP000777_GR
- KEGG:   lbf:LBF_2841
- HOGENOM:   HBG395951
- OMA:   LRYLGTC
- ProtClustDB:   PRK05477
- BioCyc:   LBIF355278:LBF_2841-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00121
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR023168
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958
- Gene3D:   G3DSA:1.10.10.410
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00133

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: 6.3.5.-

Molecular weight: Translated: 55142; Mature: 55142

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEYEVIIGLEVHVQLNTNSKIFSTATNEFGGSPNTHISPLCVALPGTLPVLNEVVLEKAV
CCEEEEEEEEEEEEECCCCEEEEECCHHCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHH
RAGVALGCEITKFTKFDRKNYFYPDLPKGYQISQFDKPYATKGGIHVLLKGEKEEKFIPL
HCCHHCCCEEHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCH
TRIHMEEDAGKLIHSHDPSINRSYVDYNRAGTPLIEIVSEPDLRSSDEAYVYLNELKTIL
HEEEEHHHHHHHHHCCCCCCCCCEECCCCCCCCCEEEECCCCCCCCCCCEEEHHHHHHHH
RYIQVSDCNMEEGSLRCDANVSIRPKGEKGFRTRVEIKNLNSFKAVKQAIDYEVEWQKDV
HHHHHCCCCCCCCCEEECCCEEECCCCCCCCEEEEEECCCHHHHHHHHHHCCCCEEHHHH
YSRGESFKQMTKLWDATLLKTIPMRSKEMSHDYRYFPEPDLPTIQISDSFIEDIRKTLPE
HHCCHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHH
LPRQKKERYKTELGLPEYDAEVLTSEREIAEYFEEALVIAGDAKKTSNWVKDEILGIVNK
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHCCC
ENISIQEFAIDPVRIGKLVKLINSGEITGKIAKTIFEDMLTTKDQPETIVEKNGLKVVRD
CCCEEHHEECCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHCCCCEEEEC
DKALEEIVIRVIESQPESVEGWKNGKDRVLGAIVGGVMKETKGKADPKLVNELILAKLGP
HHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCC
LGEKKKV
CCCCCCC
>Mature Secondary Structure
MEYEVIIGLEVHVQLNTNSKIFSTATNEFGGSPNTHISPLCVALPGTLPVLNEVVLEKAV
CCEEEEEEEEEEEEECCCCEEEEECCHHCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHH
RAGVALGCEITKFTKFDRKNYFYPDLPKGYQISQFDKPYATKGGIHVLLKGEKEEKFIPL
HCCHHCCCEEHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCH
TRIHMEEDAGKLIHSHDPSINRSYVDYNRAGTPLIEIVSEPDLRSSDEAYVYLNELKTIL
HEEEEHHHHHHHHHCCCCCCCCCEECCCCCCCCCEEEECCCCCCCCCCCEEEHHHHHHHH
RYIQVSDCNMEEGSLRCDANVSIRPKGEKGFRTRVEIKNLNSFKAVKQAIDYEVEWQKDV
HHHHHCCCCCCCCCEEECCCEEECCCCCCCCEEEEEECCCHHHHHHHHHHCCCCEEHHHH
YSRGESFKQMTKLWDATLLKTIPMRSKEMSHDYRYFPEPDLPTIQISDSFIEDIRKTLPE
HHCCHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHH
LPRQKKERYKTELGLPEYDAEVLTSEREIAEYFEEALVIAGDAKKTSNWVKDEILGIVNK
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHCCC
ENISIQEFAIDPVRIGKLVKLINSGEITGKIAKTIFEDMLTTKDQPETIVEKNGLKVVRD
CCCEEHHEECCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHCCCCEEEEC
DKALEEIVIRVIESQPESVEGWKNGKDRVLGAIVGGVMKETKGKADPKLVNELILAKLGP
HHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCC
LGEKKKV
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA