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The map label for this gene is lpdA3 [H]

Identifier: 183222268

GI number: 183222268

Start: 3016423

End: 3017850

Strand: Reverse

Name: lpdA3 [H]

Synonym: LEPBI_I2919

Alternate gene names: 183222268

Gene position: 3017850-3016423 (Counterclockwise)

Preceding gene: 183222269

Following gene: 183222267

Centisome position: 83.84

GC content: 44.05

Gene sequence:

>1428_bases
ATGTCCAATTCAAACCATTTCCAAGTGATCGTAATTGGAGGAGGGCCTGGTGGTTATGTCGCCGCCATTCGTGCCGCCCA
ACTCGGCTTACAAACATGTGTCGTCGAACGAGATAAACTCGGTGGAGTTTGTTTGAATTGGGGTTGTATTCCCACCAAAG
CCTTGTTAGAAAGTGCTCATGTTTTAGAACATCTCAAAGAAGCCGCCAAGTTTGGAATCTCTGCGGACAATATCAAAGTC
GATTTTGATGCTGTCATCAAACGTTCCCGTTCTGTCGCAGACCAAATGGCAAAGGGTGTGGAATTTTTAATGAAAAAAAA
CAAAGTCACCGTAGTGAGTGGTGAGGCAAAGTTTTTAAATTCCAAATCGATCGAAGTGAAATCAATTAGTGGCGAAGTGA
CTTCCCTGACAGCTGATTATTTTATATTAGCAGTGGGTGCCAAAAACAAAGCCCTTCCCTTTTTGCCATTTGACGGCAAA
CGCGTATTATCTGCTAGGGAAGCCATGGTAGAACCAAAGGTCATTGCTAATCTTGCCATCATTGGGGCAGGAGCGATTGG
AGTGGAATTTGCTGATTTTTATGCGAGTATGGGATCCAAAGTGAGCATCATTGAATTCCAAGACCATCTCCTTCCGAACG
AAGACAAAGAAATCTCCAGTATTTTGGAACGTAGTTTTAAAAAACGAGGGATCGAACAGTACTTAAGTTATGGAGTGGAA
ACAGCATCTGTTTCCGATTCTGGAGTTCTGCTCACCATCCAAGACAGAAATTCGGCCAAAAAAGAAAAATTAAACTTTGA
TAAGGTGATTGTGGGTGTGGGGATCTCGCCAAATACCAATGCCATTGGTCTTGATGAGATCGGAGTCAAATTAAAAAATG
GATTTGTAGAATTCACGGGTAACTACCGTTCAACAGTAGATCATATTTATGCCATCGGTGATTGTATTCCAACTCCATCC
CTTGCCCATGTGGCAAGTGCCGAAGGGATTCGTGCTGCCGAAGACATTTCGGTTCGATTGGGAAATCCCCATTTACTGCA
GATCAATCGATTGAACTATTCTTACATTCCAGGTTGTACGTATTGCCATCCAGAAGTGGCAAGTGTGGGCCTCAGTGAAG
ACAAAGCGAAAGCAATGGGACACGAACTCAAAATTGGAAAATTTCCTTTTTCTGCCAGTGGTCGTGCACAGGCACAAGGG
GATACAACGGGTATGGTCAAAATTGTTTCCGATGCCAAACATGGAGAAATTTTAGGAGCCCATATCATTGGTCCTGGAGC
GACGGAACTCATCGCAGAACTCACATTAGGTGCCAATATGGAGATCACAGTCCGGGAACTTGCAAATACGATCCATGCTC
ACCCCACACTTGCCGAAGGAATTATGGAAAGTGCTGCCGCCGTTCTTGGGGAAGCGATCAATATATAA

Upstream 100 bases:

>100_bases
TGAAAAATACATCAAAACGGGAGCCCGAATGCTCATCGTCGCAAAAAAGAGGTCTTGACTTTCCTTTTTTGTACGGTATC
TCATTCTATCGGGAGAACGT

Downstream 100 bases:

>100_bases
GGGTTAAAGCTAAGGGATAGAATAGCAATTCATTCAGAGATTCATATGGACTTCGAAAGAGTTTTTTTCTCGTTTATCAA
GTGTTATGAGACATGTTTGC

Product: alpha keto acid dehydrogenase complex E3 component dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes [H]

Number of amino acids: Translated: 475; Mature: 474

Protein sequence:

>475_residues
MSNSNHFQVIVIGGGPGGYVAAIRAAQLGLQTCVVERDKLGGVCLNWGCIPTKALLESAHVLEHLKEAAKFGISADNIKV
DFDAVIKRSRSVADQMAKGVEFLMKKNKVTVVSGEAKFLNSKSIEVKSISGEVTSLTADYFILAVGAKNKALPFLPFDGK
RVLSAREAMVEPKVIANLAIIGAGAIGVEFADFYASMGSKVSIIEFQDHLLPNEDKEISSILERSFKKRGIEQYLSYGVE
TASVSDSGVLLTIQDRNSAKKEKLNFDKVIVGVGISPNTNAIGLDEIGVKLKNGFVEFTGNYRSTVDHIYAIGDCIPTPS
LAHVASAEGIRAAEDISVRLGNPHLLQINRLNYSYIPGCTYCHPEVASVGLSEDKAKAMGHELKIGKFPFSASGRAQAQG
DTTGMVKIVSDAKHGEILGAHIIGPGATELIAELTLGANMEITVRELANTIHAHPTLAEGIMESAAAVLGEAINI

Sequences:

>Translated_475_residues
MSNSNHFQVIVIGGGPGGYVAAIRAAQLGLQTCVVERDKLGGVCLNWGCIPTKALLESAHVLEHLKEAAKFGISADNIKV
DFDAVIKRSRSVADQMAKGVEFLMKKNKVTVVSGEAKFLNSKSIEVKSISGEVTSLTADYFILAVGAKNKALPFLPFDGK
RVLSAREAMVEPKVIANLAIIGAGAIGVEFADFYASMGSKVSIIEFQDHLLPNEDKEISSILERSFKKRGIEQYLSYGVE
TASVSDSGVLLTIQDRNSAKKEKLNFDKVIVGVGISPNTNAIGLDEIGVKLKNGFVEFTGNYRSTVDHIYAIGDCIPTPS
LAHVASAEGIRAAEDISVRLGNPHLLQINRLNYSYIPGCTYCHPEVASVGLSEDKAKAMGHELKIGKFPFSASGRAQAQG
DTTGMVKIVSDAKHGEILGAHIIGPGATELIAELTLGANMEITVRELANTIHAHPTLAEGIMESAAAVLGEAINI
>Mature_474_residues
SNSNHFQVIVIGGGPGGYVAAIRAAQLGLQTCVVERDKLGGVCLNWGCIPTKALLESAHVLEHLKEAAKFGISADNIKVD
FDAVIKRSRSVADQMAKGVEFLMKKNKVTVVSGEAKFLNSKSIEVKSISGEVTSLTADYFILAVGAKNKALPFLPFDGKR
VLSAREAMVEPKVIANLAIIGAGAIGVEFADFYASMGSKVSIIEFQDHLLPNEDKEISSILERSFKKRGIEQYLSYGVET
ASVSDSGVLLTIQDRNSAKKEKLNFDKVIVGVGISPNTNAIGLDEIGVKLKNGFVEFTGNYRSTVDHIYAIGDCIPTPSL
AHVASAEGIRAAEDISVRLGNPHLLQINRLNYSYIPGCTYCHPEVASVGLSEDKAKAMGHELKIGKFPFSASGRAQAQGD
TTGMVKIVSDAKHGEILGAHIIGPGATELIAELTLGANMEITVRELANTIHAHPTLAEGIMESAAAVLGEAINI

Specific function: Lipoamide dehydrogenase is a component of the alpha- ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=474, Percent_Identity=36.2869198312236, Blast_Score=291, Evalue=8e-79,
Organism=Homo sapiens, GI50301238, Length=465, Percent_Identity=25.8064516129032, Blast_Score=136, Evalue=5e-32,
Organism=Homo sapiens, GI33519430, Length=456, Percent_Identity=25, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI33519428, Length=456, Percent_Identity=25, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI33519426, Length=456, Percent_Identity=25, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI148277071, Length=456, Percent_Identity=25, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI148277065, Length=456, Percent_Identity=25, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI291045266, Length=438, Percent_Identity=23.972602739726, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI22035672, Length=475, Percent_Identity=24.2105263157895, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI291045268, Length=430, Percent_Identity=22.5581395348837, Blast_Score=100, Evalue=2e-21,
Organism=Escherichia coli, GI1786307, Length=463, Percent_Identity=36.2850971922246, Blast_Score=280, Evalue=1e-76,
Organism=Escherichia coli, GI87082354, Length=477, Percent_Identity=29.1404612159329, Blast_Score=192, Evalue=3e-50,
Organism=Escherichia coli, GI1789915, Length=442, Percent_Identity=27.6018099547511, Blast_Score=152, Evalue=5e-38,
Organism=Escherichia coli, GI87081717, Length=476, Percent_Identity=25.8403361344538, Blast_Score=142, Evalue=7e-35,
Organism=Caenorhabditis elegans, GI32565766, Length=478, Percent_Identity=37.2384937238494, Blast_Score=295, Evalue=3e-80,
Organism=Caenorhabditis elegans, GI17557007, Length=483, Percent_Identity=24.223602484472, Blast_Score=108, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI71983429, Length=481, Percent_Identity=27.6507276507277, Blast_Score=104, Evalue=9e-23,
Organism=Caenorhabditis elegans, GI71983419, Length=483, Percent_Identity=27.9503105590062, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI71982272, Length=461, Percent_Identity=23.8611713665944, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17559934, Length=326, Percent_Identity=25.4601226993865, Blast_Score=72, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6321091, Length=484, Percent_Identity=38.6363636363636, Blast_Score=298, Evalue=1e-81,
Organism=Saccharomyces cerevisiae, GI6325240, Length=472, Percent_Identity=26.9067796610169, Blast_Score=172, Evalue=8e-44,
Organism=Saccharomyces cerevisiae, GI6325166, Length=478, Percent_Identity=26.9874476987448, Blast_Score=142, Evalue=8e-35,
Organism=Drosophila melanogaster, GI21358499, Length=479, Percent_Identity=37.5782881002088, Blast_Score=315, Evalue=3e-86,
Organism=Drosophila melanogaster, GI17737741, Length=479, Percent_Identity=26.0960334029228, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24640549, Length=478, Percent_Identity=25.1046025104602, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24640553, Length=477, Percent_Identity=25.1572327044025, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24640551, Length=475, Percent_Identity=25.2631578947368, Blast_Score=123, Evalue=3e-28,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 50542; Mature: 50410

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNSNHFQVIVIGGGPGGYVAAIRAAQLGLQTCVVERDKLGGVCLNWGCIPTKALLESAH
CCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHEECCCCCCEEEECCCCCHHHHHHHHH
VLEHLKEAAKFGISADNIKVDFDAVIKRSRSVADQMAKGVEFLMKKNKVTVVSGEAKFLN
HHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHCCC
SKSIEVKSISGEVTSLTADYFILAVGAKNKALPFLPFDGKRVLSAREAMVEPKVIANLAI
CCCEEEEECCCCCEEEEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHEEEE
IGAGAIGVEFADFYASMGSKVSIIEFQDHLLPNEDKEISSILERSFKKRGIEQYLSYGVE
EECCHHHHHHHHHHHHCCCEEEEEEEHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
TASVSDSGVLLTIQDRNSAKKEKLNFDKVIVGVGISPNTNAIGLDEIGVKLKNGFVEFTG
EECCCCCCEEEEEECCCCCHHHHCCCCEEEEEECCCCCCCEEEHHHHCEEEECCEEEEEC
NYRSTVDHIYAIGDCIPTPSLAHVASAEGIRAAEDISVRLGNPHLLQINRLNYSYIPGCT
CHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCEEEECCCEEEEEECCCEEECCCCC
YCHPEVASVGLSEDKAKAMGHELKIGKFPFSASGRAQAQGDTTGMVKIVSDAKHGEILGA
CCCHHHHHCCCCHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCCCEEEE
HIIGPGATELIAELTLGANMEITVRELANTIHAHPTLAEGIMESAAAVLGEAINI
EEECCCHHHHHHHHHCCCCCEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SNSNHFQVIVIGGGPGGYVAAIRAAQLGLQTCVVERDKLGGVCLNWGCIPTKALLESAH
CCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHEECCCCCCEEEECCCCCHHHHHHHHH
VLEHLKEAAKFGISADNIKVDFDAVIKRSRSVADQMAKGVEFLMKKNKVTVVSGEAKFLN
HHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHCCC
SKSIEVKSISGEVTSLTADYFILAVGAKNKALPFLPFDGKRVLSAREAMVEPKVIANLAI
CCCEEEEECCCCCEEEEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHEEEE
IGAGAIGVEFADFYASMGSKVSIIEFQDHLLPNEDKEISSILERSFKKRGIEQYLSYGVE
EECCHHHHHHHHHHHHCCCEEEEEEEHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
TASVSDSGVLLTIQDRNSAKKEKLNFDKVIVGVGISPNTNAIGLDEIGVKLKNGFVEFTG
EECCCCCCEEEEEECCCCCHHHHCCCCEEEEEECCCCCCCEEEHHHHCEEEECCEEEEEC
NYRSTVDHIYAIGDCIPTPSLAHVASAEGIRAAEDISVRLGNPHLLQINRLNYSYIPGCT
CHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCEEEECCCEEEEEECCCEEECCCCC
YCHPEVASVGLSEDKAKAMGHELKIGKFPFSASGRAQAQGDTTGMVKIVSDAKHGEILGA
CCCHHHHHCCCCHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCCCEEEE
HIIGPGATELIAELTLGANMEITVRELANTIHAHPTLAEGIMESAAAVLGEAINI
EEECCCHHHHHHHHHCCCCCEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9515924 [H]