Click here to switch to the map view.

The map label for this gene is fixL [H]

Identifier: 183221811

GI number: 183221811

Start: 2515069

End: 2517225

Strand: Reverse

Name: fixL [H]

Synonym: LEPBI_I2442

Alternate gene names: 183221811

Gene position: 2517225-2515069 (Counterclockwise)

Preceding gene: 183221812

Following gene: 183221810

Centisome position: 69.93

GC content: 35.19

Gene sequence:

>2157_bases
ATGACAGTTGTAGCAGATAAATCGATTTTACTTGTCGAAGATGAAGCCATACTGGCTCTTTACGAAAAAAATCAACTGGA
GCAAGGTGGGTATAAAGTCACTCATGTTCCCAGTGGTGAAAATGCAATCCGACTGGTCATGCAAGATGAGAATCCTTTTG
ATCTGATCCTTATGGATATCGACTTGGGTAAAGGATTAGATGGAACAGAGACTGCAACGCACATTCTCAATCATAAAGAA
ATACCAATCGTATTTTTATCTTCACATACAGAAAGAGAAATCGTCAAAAAAACAGAGAGTATCACTTCTTATGGATATGT
AGTAAAAAATTCCGGGTTCACTGTACTTGATGCTTCGATTAAAATGGCGTTCAAACTATTTGAGGCAAACGAATTAACAA
AAAGTAAAAAAGAACATTTAGAAACCGTTTTACATTCCATAGGAGATGGAGTGATTGCTACCGATAAAGAAGGAAAAGTC
ATACGTATCAATCCTGTCGCCGAAAAATTAACGGGTTGGTCTCACGATGATGGGCTTGGTCTAGAGATTAACAAAGTGTT
TAATATCATCAATGTAAAAACTCGGCACAAAGTTGATAATCCTGTCGATATTGTATTAAAAACTAATTCTGTTGTTGCAT
TAGCAAATCATACTGTACTCATCTCAAAAGATGGAGCTGAATATCAAATTTCGGATTCAGGATCACCGATTAAAGACCTA
AATGGTGAAACAAAAGGAGTTGTACTTGTATTTCGAGATATTACCGCGGAATACAATGTCCAAAGCCAAATTGCCAAACA
AGCAAACATGCTCAATAATGTTTTGGATGCTGTGATAGGCACTGATTTCACACAAAAAATCAATTATTGGAACAAGGCGG
CTGAGAAAATTTTCCTTTGGAAAGCGGAAGAAGTCATCGGGGAAAATATTTTGGAAGTTTTGAAAACAAAATATCTCAAT
TTTACAAACCAAGAAATCATTGAGAAAGTGAATTTAGAAGGAAGTTATATTGGTGAAGCGATTCAAGAAGCAAAGGGTGG
ATGTGTTCGGAACATAGAAATTAATTTAGTACTCTTAAATGATGAATTTGATTTACCCACTGGTTATATTTTTGTAGCAA
GAGACATTTCTGATCGATTGAAGGCACTCAATCAAATTAAGGAATCGGAAGCAAAATTAACGCAAATCATAGACTCTGCA
ATGGATGCGATCATCAGTATCGATAATACAAAAAAGATTATTTTGTTCAATGGTGCAGCCGAAAAAATGTTTGGATATGA
ATCAGACGCAATCATCGGAAAATCTTTAGACCAATTGATTCCAATTAATTTCAGAAGCCAACATGATAAACATATTGATC
AATTTAGTAAAACTGGTGTTAGCCGAAGGGCAATGGGTGCATTGGGTGAGATCAGTGGTTTACGTGCCAATGGTGAAGAA
TTCCCAATCGAAGCTTCTATTTCCCAAATCACGGTAAATGGAGAATCATTATTTACTGTAATTTTAAGGGACGTAAGCGT
ACGAACATTATCTGAATCTAAAATTCAGAAGTTGCTTCACGAAAAAGAAAATATTTTAAAAGAGATTCACCATCGTGTGA
AAAATAACATGAGTTCATTGTTCACCTTGCTCACGTTACAAGCTAAATCTCAAAACCATGAGCCAGTACAAACCATTTTG
TATGAAGCTGCGGGCAGAATTAAAAGTATGATTGTTCTCTATGACAAACTATACCATTCTGAAACCGACAATAGTGTCTC
CATACAAAATTACTTTCCTTCAATTTGTTCTGAAATCGTATCTATTTTCCCGAAAAAAGTGGAAGTGAACCTCTCTATAC
TCGAGGAACCGATCGAATTGAACGCTAAGTTGCTTTCTTCATTAGGCATTATTTTAAATGAATTGATCACCAATTCCATG
AAACATGCTTTTTTGGAAATTCAGAATCCGAAGATTAATTTAAATATCTCCAAAGATAATAACATTTTGTACTTAGCGTA
TTCAGACAATGGAGTGGGGATTCCGGAATCAATATCGTTTCAAGACACACCAGGATTTGGTTTACAACTCATCGGAATGC
TCACTGAACAAATTGAAGGTAAAATCAGTATCGATCGTAAAAATGAACCAAAGATTATTTTGGAATTGAAACTATAA

Upstream 100 bases:

>100_bases
TTCTAAATTATTGGTTGTAATTCAAATTCTCTAGTCCATGATTTGGAGTTCGGTATTTCGATGCGTTCATTGATTCGTCA
AAATACCAAAAGGATGAAGG

Downstream 100 bases:

>100_bases
CAAATACTTTTTTTCTTTGTTTGAATAGAATATTAGTTTACCTTCTTATCACTTCTACTTCCATTCACCCATGGGCAAAC
TTCAATTTTTTGTAGTTTTA

Product: putative two-component response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 718; Mature: 717

Protein sequence:

>718_residues
MTVVADKSILLVEDEAILALYEKNQLEQGGYKVTHVPSGENAIRLVMQDENPFDLILMDIDLGKGLDGTETATHILNHKE
IPIVFLSSHTEREIVKKTESITSYGYVVKNSGFTVLDASIKMAFKLFEANELTKSKKEHLETVLHSIGDGVIATDKEGKV
IRINPVAEKLTGWSHDDGLGLEINKVFNIINVKTRHKVDNPVDIVLKTNSVVALANHTVLISKDGAEYQISDSGSPIKDL
NGETKGVVLVFRDITAEYNVQSQIAKQANMLNNVLDAVIGTDFTQKINYWNKAAEKIFLWKAEEVIGENILEVLKTKYLN
FTNQEIIEKVNLEGSYIGEAIQEAKGGCVRNIEINLVLLNDEFDLPTGYIFVARDISDRLKALNQIKESEAKLTQIIDSA
MDAIISIDNTKKIILFNGAAEKMFGYESDAIIGKSLDQLIPINFRSQHDKHIDQFSKTGVSRRAMGALGEISGLRANGEE
FPIEASISQITVNGESLFTVILRDVSVRTLSESKIQKLLHEKENILKEIHHRVKNNMSSLFTLLTLQAKSQNHEPVQTIL
YEAAGRIKSMIVLYDKLYHSETDNSVSIQNYFPSICSEIVSIFPKKVEVNLSILEEPIELNAKLLSSLGIILNELITNSM
KHAFLEIQNPKINLNISKDNNILYLAYSDNGVGIPESISFQDTPGFGLQLIGMLTEQIEGKISIDRKNEPKIILELKL

Sequences:

>Translated_718_residues
MTVVADKSILLVEDEAILALYEKNQLEQGGYKVTHVPSGENAIRLVMQDENPFDLILMDIDLGKGLDGTETATHILNHKE
IPIVFLSSHTEREIVKKTESITSYGYVVKNSGFTVLDASIKMAFKLFEANELTKSKKEHLETVLHSIGDGVIATDKEGKV
IRINPVAEKLTGWSHDDGLGLEINKVFNIINVKTRHKVDNPVDIVLKTNSVVALANHTVLISKDGAEYQISDSGSPIKDL
NGETKGVVLVFRDITAEYNVQSQIAKQANMLNNVLDAVIGTDFTQKINYWNKAAEKIFLWKAEEVIGENILEVLKTKYLN
FTNQEIIEKVNLEGSYIGEAIQEAKGGCVRNIEINLVLLNDEFDLPTGYIFVARDISDRLKALNQIKESEAKLTQIIDSA
MDAIISIDNTKKIILFNGAAEKMFGYESDAIIGKSLDQLIPINFRSQHDKHIDQFSKTGVSRRAMGALGEISGLRANGEE
FPIEASISQITVNGESLFTVILRDVSVRTLSESKIQKLLHEKENILKEIHHRVKNNMSSLFTLLTLQAKSQNHEPVQTIL
YEAAGRIKSMIVLYDKLYHSETDNSVSIQNYFPSICSEIVSIFPKKVEVNLSILEEPIELNAKLLSSLGIILNELITNSM
KHAFLEIQNPKINLNISKDNNILYLAYSDNGVGIPESISFQDTPGFGLQLIGMLTEQIEGKISIDRKNEPKIILELKL
>Mature_717_residues
TVVADKSILLVEDEAILALYEKNQLEQGGYKVTHVPSGENAIRLVMQDENPFDLILMDIDLGKGLDGTETATHILNHKEI
PIVFLSSHTEREIVKKTESITSYGYVVKNSGFTVLDASIKMAFKLFEANELTKSKKEHLETVLHSIGDGVIATDKEGKVI
RINPVAEKLTGWSHDDGLGLEINKVFNIINVKTRHKVDNPVDIVLKTNSVVALANHTVLISKDGAEYQISDSGSPIKDLN
GETKGVVLVFRDITAEYNVQSQIAKQANMLNNVLDAVIGTDFTQKINYWNKAAEKIFLWKAEEVIGENILEVLKTKYLNF
TNQEIIEKVNLEGSYIGEAIQEAKGGCVRNIEINLVLLNDEFDLPTGYIFVARDISDRLKALNQIKESEAKLTQIIDSAM
DAIISIDNTKKIILFNGAAEKMFGYESDAIIGKSLDQLIPINFRSQHDKHIDQFSKTGVSRRAMGALGEISGLRANGEEF
PIEASISQITVNGESLFTVILRDVSVRTLSESKIQKLLHEKENILKEIHHRVKNNMSSLFTLLTLQAKSQNHEPVQTILY
EAAGRIKSMIVLYDKLYHSETDNSVSIQNYFPSICSEIVSIFPKKVEVNLSILEEPIELNAKLLSSLGIILNELITNSMK
HAFLEIQNPKINLNISKDNNILYLAYSDNGVGIPESISFQDTPGFGLQLIGMLTEQIEGKISIDRKNEPKIILELKL

Specific function: Putative oxygen sensor; modulates the activity of fixJ, a transcriptional activator of nitrogen fixation fixK gene. FixL probably acts as a kinase that phosphorylates fixJ [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1788381, Length=142, Percent_Identity=30.9859154929577, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI87081921, Length=123, Percent_Identity=25.2032520325203, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 80041; Mature: 79910

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVVADKSILLVEDEAILALYEKNQLEQGGYKVTHVPSGENAIRLVMQDENPFDLILMDI
CEEEECCEEEEEECCEEEEEEECCCCCCCCEEEEECCCCCCEEEEEEECCCCEEEEEEEE
DLGKGLDGTETATHILNHKEIPIVFLSSHTEREIVKKTESITSYGYVVKNSGFTVLDASI
ECCCCCCCHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHCCEEEECCCCEEEEHHH
KMAFKLFEANELTKSKKEHLETVLHSIGDGVIATDKEGKVIRINPVAEKLTGWSHDDGLG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEEHHHHHHCCCCCCCCCC
LEINKVFNIINVKTRHKVDNPVDIVLKTNSVVALANHTVLISKDGAEYQISDSGSPIKDL
EEHHHHHHEHEEEHHCCCCCCEEEEEECCCEEEEECEEEEEECCCCEEEECCCCCCHHHC
NGETKGVVLVFRDITAEYNVQSQIAKQANMLNNVLDAVIGTDFTQKINYWNKAAEKIFLW
CCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEEEE
KAEEVIGENILEVLKTKYLNFTNQEIIEKVNLEGSYIGEAIQEAKGGCVRNIEINLVLLN
EHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHCCCEEEEEEEEEEEEC
DEFDLPTGYIFVARDISDRLKALNQIKESEAKLTQIIDSAMDAIISIDNTKKIILFNGAA
CCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCEEEEECCCH
EKMFGYESDAIIGKSLDQLIPINFRSQHDKHIDQFSKTGVSRRAMGALGEISGLRANGEE
HHHCCCCCCCHHHCCHHHEECCCCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCC
FPIEASISQITVNGESLFTVILRDVSVRTLSESKIQKLLHEKENILKEIHHRVKNNMSSL
CCEEEEEEEEEECCHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FTLLTLQAKSQNHEPVQTILYEAAGRIKSMIVLYDKLYHSETDNSVSIQNYFPSICSEIV
HHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHH
SIFPKKVEVNLSILEEPIELNAKLLSSLGIILNELITNSMKHAFLEIQNPKINLNISKDN
HHCCCCEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCEEEEEEECCC
NILYLAYSDNGVGIPESISFQDTPGFGLQLIGMLTEQIEGKISIDRKNEPKIILELKL
CEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCEEEEEEEC
>Mature Secondary Structure 
TVVADKSILLVEDEAILALYEKNQLEQGGYKVTHVPSGENAIRLVMQDENPFDLILMDI
EEEECCEEEEEECCEEEEEEECCCCCCCCEEEEECCCCCCEEEEEEECCCCEEEEEEEE
DLGKGLDGTETATHILNHKEIPIVFLSSHTEREIVKKTESITSYGYVVKNSGFTVLDASI
ECCCCCCCHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHCCEEEECCCCEEEEHHH
KMAFKLFEANELTKSKKEHLETVLHSIGDGVIATDKEGKVIRINPVAEKLTGWSHDDGLG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEEHHHHHHCCCCCCCCCC
LEINKVFNIINVKTRHKVDNPVDIVLKTNSVVALANHTVLISKDGAEYQISDSGSPIKDL
EEHHHHHHEHEEEHHCCCCCCEEEEEECCCEEEEECEEEEEECCCCEEEECCCCCCHHHC
NGETKGVVLVFRDITAEYNVQSQIAKQANMLNNVLDAVIGTDFTQKINYWNKAAEKIFLW
CCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEEEE
KAEEVIGENILEVLKTKYLNFTNQEIIEKVNLEGSYIGEAIQEAKGGCVRNIEINLVLLN
EHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHCCCEEEEEEEEEEEEC
DEFDLPTGYIFVARDISDRLKALNQIKESEAKLTQIIDSAMDAIISIDNTKKIILFNGAA
CCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCEEEEECCCH
EKMFGYESDAIIGKSLDQLIPINFRSQHDKHIDQFSKTGVSRRAMGALGEISGLRANGEE
HHHCCCCCCCHHHCCHHHEECCCCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCC
FPIEASISQITVNGESLFTVILRDVSVRTLSESKIQKLLHEKENILKEIHHRVKNNMSSL
CCEEEEEEEEEECCHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FTLLTLQAKSQNHEPVQTILYEAAGRIKSMIVLYDKLYHSETDNSVSIQNYFPSICSEIV
HHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHH
SIFPKKVEVNLSILEEPIELNAKLLSSLGIILNELITNSMKHAFLEIQNPKINLNISKDN
HHCCCCEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCEEEEEEECCC
NILYLAYSDNGVGIPESISFQDTPGFGLQLIGMLTEQIEGKISIDRKNEPKIILELKL
CEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2842062; 11481432; 8432704 [H]