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The map label for this gene is pyrE

Identifier: 183221797

GI number: 183221797

Start: 2502360

End: 2502917

Strand: Reverse

Name: pyrE

Synonym: LEPBI_I2428

Alternate gene names: 183221797

Gene position: 2502917-2502360 (Counterclockwise)

Preceding gene: 183221799

Following gene: 183221796

Centisome position: 69.53

GC content: 39.61

Gene sequence:

>558_bases
ATGTCCCATTCACACCGCGATGCATTATTCCAATGGATGAAAACTTACGTCTATCGTCATTCCGAGACACCGTTTCGGTT
GGCAAGCGGTCTGGAATCCCAACATTATTTTAATTGTAAAGAGATCACGCTTCATCCTGAAAGATTATCCATCTTAGCAG
AATGTTTCATAGAGGAAATCATTCCGAAATTAAACATTGAATTCCAAGCAGTAGGTGGTTTGACACTTGGTGCAGACCCA
ATCGCCTATGCAATTTCACTTGGATATCAAAAGAGAGGGAAGAATGTTTTTCCACTCGTTGTGAGAAAAGAATCCAAAGG
GCATGGAACAGGCCAACAAATTGAAGGATTCTGGAAGGATATCAAAACTTGCCTTGTGGTTGATGATGTCATCACCACTG
GAGGTTCTACTCTAAAAGCCGTAAAAGTTTTAAGAGAAGTTGGGATAAATGTTACCAAAGGGATATGCATCTTAGATAGA
GAAGAGGGTGGAAGTGAAAATTTGCAGACAGAGAATGTAACGATGACATCTATATTTGCCAAAAGTGAGTTTTTCTAA

Upstream 100 bases:

>100_bases
AAGTGTGTTGATTTTTTGATGGTTGCTAGGCGATCCATTTGGAATTCAAGCCGAGCCTAAAAATTTGTATTCATAGTAAA
CAGTGGAAAATAATTAGAAT

Downstream 100 bases:

>100_bases
TGAGTGAAAAAAAAATATTTTTATACAATGTTGGTATATTTCGGAAAAAACTACTAATTAGAACCTGGGCAGTCATCGTA
TTATTCGTTTTATTTGTGAT

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT; OPRTase

Number of amino acids: Translated: 185; Mature: 184

Protein sequence:

>185_residues
MSHSHRDALFQWMKTYVYRHSETPFRLASGLESQHYFNCKEITLHPERLSILAECFIEEIIPKLNIEFQAVGGLTLGADP
IAYAISLGYQKRGKNVFPLVVRKESKGHGTGQQIEGFWKDIKTCLVVDDVITTGGSTLKAVKVLREVGINVTKGICILDR
EEGGSENLQTENVTMTSIFAKSEFF

Sequences:

>Translated_185_residues
MSHSHRDALFQWMKTYVYRHSETPFRLASGLESQHYFNCKEITLHPERLSILAECFIEEIIPKLNIEFQAVGGLTLGADP
IAYAISLGYQKRGKNVFPLVVRKESKGHGTGQQIEGFWKDIKTCLVVDDVITTGGSTLKAVKVLREVGINVTKGICILDR
EEGGSENLQTENVTMTSIFAKSEFF
>Mature_184_residues
SHSHRDALFQWMKTYVYRHSETPFRLASGLESQHYFNCKEITLHPERLSILAECFIEEIIPKLNIEFQAVGGLTLGADPI
AYAISLGYQKRGKNVFPLVVRKESKGHGTGQQIEGFWKDIKTCLVVDDVITTGGSTLKAVKVLREVGINVTKGICILDRE
EGGSENLQTENVTMTSIFAKSEFF

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP)

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily

Homologues:

Organism=Homo sapiens, GI4507835, Length=162, Percent_Identity=34.5679012345679, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17554964, Length=174, Percent_Identity=35.0574712643678, Blast_Score=97, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17508631, Length=155, Percent_Identity=33.5483870967742, Blast_Score=86, Evalue=1e-17,
Organism=Drosophila melanogaster, GI17933654, Length=158, Percent_Identity=34.1772151898734, Blast_Score=81, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PYRE_LEPBA (B0SCV6)

Other databases:

- EMBL:   CP000777
- RefSeq:   YP_001963426.1
- ProteinModelPortal:   B0SCV6
- SMR:   B0SCV6
- GeneID:   6388775
- GenomeReviews:   CP000777_GR
- KEGG:   lbf:LBF_2358
- HOGENOM:   HBG404341
- OMA:   GKEADYY
- ProtClustDB:   CLSK574681
- BioCyc:   LBIF355278:LBF_2358-MONOMER
- HAMAP:   MF_01208
- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836
- TIGRFAMs:   TIGR00336

Pfam domain/function: PF00156 Pribosyltran

EC number: =2.4.2.10

Molecular weight: Translated: 20755; Mature: 20624

Theoretical pI: Translated: 7.11; Mature: 7.11

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: BINDING 102-102 BINDING 103-103 BINDING 106-106 BINDING 108-108 BINDING 132-132 BINDING 160-160

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHSHRDALFQWMKTYVYRHSETPFRLASGLESQHYFNCKEITLHPERLSILAECFIEEI
CCCCHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCHHEEECHHHHHHHHHHHHHHH
IPKLNIEFQAVGGLTLGADPIAYAISLGYQKRGKNVFPLVVRKESKGHGTGQQIEGFWKD
HHHCCCEEEEECCEEECCCHHHHHHHHCHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHH
IKTCLVVDDVITTGGSTLKAVKVLREVGINVTKGICILDREEGGSENLQTENVTMTSIFA
HHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEECCEEEEECCCCCCCCCCCCCEEEEEEEC
KSEFF
CCCCC
>Mature Secondary Structure 
SHSHRDALFQWMKTYVYRHSETPFRLASGLESQHYFNCKEITLHPERLSILAECFIEEI
CCCHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCHHEEECHHHHHHHHHHHHHHH
IPKLNIEFQAVGGLTLGADPIAYAISLGYQKRGKNVFPLVVRKESKGHGTGQQIEGFWKD
HHHCCCEEEEECCEEECCCHHHHHHHHCHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHH
IKTCLVVDDVITTGGSTLKAVKVLREVGINVTKGICILDREEGGSENLQTENVTMTSIFA
HHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEECCEEEEECCCCCCCCCCCCCEEEEEEEC
KSEFF
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA