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The map label for this gene is prfA [H]

Identifier: 183221718

GI number: 183221718

Start: 2418214

End: 2418546

Strand: Reverse

Name: prfA [H]

Synonym: LEPBI_I2347

Alternate gene names: 183221718

Gene position: 2418546-2418214 (Counterclockwise)

Preceding gene: 183221719

Following gene: 183221712

Centisome position: 67.19

GC content: 45.05

Gene sequence:

>333_bases
ATGGAGGCACTTGGCATCCGCGAGTCTGATCTAAAAGAACAATTTGTGAAGGCGAGTGGGAAAGGGGGACAAAACGTCAA
TAAGGTGGCAACCGCTGTTGTGTTACTCCACCTTCCTACTGGCAAACAAGTGAAGTGTAGTATTTACCGCAGCCAAGGGC
TCAACCGTTACAAAGCCCGTGATCTACTTTGTTTGGAACTAGAGAAAGAACAGAGACCCGAAAGCTTCCGACAGAAACTT
GGTGCCATCCGAAAACAAAAAGCCAAACAAAAGAAACGTTCAGTGGAGAAATATAAAGAAACGAGTATCGAAAATGGCGA
AACCGAAGATTGA

Upstream 100 bases:

>100_bases
GAAACGGCTTCTGATTTGCGGAAACGCATTTTAGAAATTGAATCCACTGCCACTTAACTTTCCAGTCTCTATTGAAAAAA
ACGCCTCCATCAAAAAACGA

Downstream 100 bases:

>100_bases
ATCAGAGTGAGTTCTCAAAATTTGGTCCAATCCATCAAATGTTTGGTAAGTTAGCAGAAAGGCGCTGTTATGCGATGTTT
TTTTAATTAGAAAAGTAGGT

Product: putative translation release factor

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 110; Mature: 110

Protein sequence:

>110_residues
MEALGIRESDLKEQFVKASGKGGQNVNKVATAVVLLHLPTGKQVKCSIYRSQGLNRYKARDLLCLELEKEQRPESFRQKL
GAIRKQKAKQKKRSVEKYKETSIENGETED

Sequences:

>Translated_110_residues
MEALGIRESDLKEQFVKASGKGGQNVNKVATAVVLLHLPTGKQVKCSIYRSQGLNRYKARDLLCLELEKEQRPESFRQKL
GAIRKQKAKQKKRSVEKYKETSIENGETED
>Mature_110_residues
MEALGIRESDLKEQFVKASGKGGQNVNKVATAVVLLHLPTGKQVKCSIYRSQGLNRYKARDLLCLELEKEQRPESFRQKL
GAIRKQKAKQKKRSVEKYKETSIENGETED

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Drosophila melanogaster, GI24654113, Length=96, Percent_Identity=39.5833333333333, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 12507; Mature: 12507

Theoretical pI: Translated: 10.34; Mature: 10.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEALGIRESDLKEQFVKASGKGGQNVNKVATAVVLLHLPTGKQVKCSIYRSQGLNRYKAR
CCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCEEEHHHHHCCCCHHHHH
DLLCLELEKEQRPESFRQKLGAIRKQKAKQKKRSVEKYKETSIENGETED
HHHHEECCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MEALGIRESDLKEQFVKASGKGGQNVNKVATAVVLLHLPTGKQVKCSIYRSQGLNRYKAR
CCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCEEEHHHHHCCCCHHHHH
DLLCLELEKEQRPESFRQKLGAIRKQKAKQKKRSVEKYKETSIENGETED
HHHHEECCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA