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The map label for this gene is nifS1 [H]

Identifier: 183221488

GI number: 183221488

Start: 2200613

End: 2201743

Strand: Direct

Name: nifS1 [H]

Synonym: LEPBI_I2106

Alternate gene names: 183221488

Gene position: 2200613-2201743 (Clockwise)

Preceding gene: 183221487

Following gene: 183221489

Centisome position: 61.13

GC content: 36.6

Gene sequence:

>1131_bases
ATGAAATTCCATTCAACGAATGAAATAAAGTATTTTGATTATAATTCAACCCACCCTCCCTATCACGAGATTTTAGAATC
TTGTTTAGAAGAGTATTTTGATAGTTTTTACAATCCTTCTGGAATCACTCGTTTTTCCTTAAAAAACCAAGGGAAAATAG
AACAAACGAGAAAGTATTTGAGCCAAATCACAGGTGGTAAAGAAAAACAATTTGTTTTTTCTTCCACGGGTACGGAAGCA
AACTACGCCCTCATCCAAAGTTTACGAGTGATGCAGCCAACACTCGCTTCCGTCATCGTATCTCCCTTTGAGCATTCAAG
TATGTATGCAGCGCTTGGTTCCTATGGATTTGAACCAATCCTAATCCAAACCCATCGATCTGGTGTGATTGATTTAAACC
ACCTTAAAACTTTATTACAAACACATCCAAGCCCTGTGATTTGTTTGTATGCAGGAAATGAAACGGGTGTCATCCAACCA
GCAGAAGAGATTCGATCTCTCACAAAACAATATGGCCAGTTGTTTTTGAGTGATTTGATGCAAGCGTTTTGTAAAATCCC
TGTTCCATTCCATTTATTTGACGGTTATACTTTTTCAGGTCATAAAATCGGTGCGGGAATGGGAATTGCTGTCACTTACT
TTCCAGAAGAGAACAAAGAGTTTCGTTTGTTTGGTGGAGGGAACCAAGAAAATGAACATAGAGCCGGAACCGAAAATACA
TTTGCCATTTCTTGTTTTCAAAAAGTGACAGAAATTCAATTGGCAAATCTGGAAGAAAAAAATGTCCGCTTACAAACATT
TCAAACCTTATTGGAATCAAACTTGGAATCGTTTGGCTGTGAAATCATTGCAAAAGAGGAAAGGAGACTTCCCAATACAA
CTTTTCTCATTTTACCGATTCAAACTGTAGATTTTTTTCTACTGGGACTAGAAGAAAAAGGAATGATTGTTTCGACAGGA
AGTTCTTGTAAATCAAGGGCAAGGGAAGCATCCAAATCATTATTACATATGGGTTATTCAGAAGAAGAAGCACTTCGAGC
GATCCGTATTTCAACCGGTAGTTTTACTAAATTAGAAGATGTAAATTTACTAATAGACCAAATGAACCAATTGATACAAA
AATTAATATAG

Upstream 100 bases:

>100_bases
GACGAACGAAGCGTTGGTGGAAAAAAAGATGAAATTCTAAAAGCATATCACAATTTAAATCGATCCGTTTTGCGTAAGCT
CGGTCGTTACGAAGAAGCAG

Downstream 100 bases:

>100_bases
TCACAGTATGAAAATTCGTATCCTATCCAAATACTCCAACCAAGGAATTTCCGATGGTGAATGTTTTTGGGAAGAAAAAC
AAAACCAATTTGGAACAGTT

Product: aminotransferase class-V protein

Products: NA

Alternate protein names: Nitrogenase metalloclusters biosynthesis protein nifS1 [H]

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MKFHSTNEIKYFDYNSTHPPYHEILESCLEEYFDSFYNPSGITRFSLKNQGKIEQTRKYLSQITGGKEKQFVFSSTGTEA
NYALIQSLRVMQPTLASVIVSPFEHSSMYAALGSYGFEPILIQTHRSGVIDLNHLKTLLQTHPSPVICLYAGNETGVIQP
AEEIRSLTKQYGQLFLSDLMQAFCKIPVPFHLFDGYTFSGHKIGAGMGIAVTYFPEENKEFRLFGGGNQENEHRAGTENT
FAISCFQKVTEIQLANLEEKNVRLQTFQTLLESNLESFGCEIIAKEERRLPNTTFLILPIQTVDFFLLGLEEKGMIVSTG
SSCKSRAREASKSLLHMGYSEEEALRAIRISTGSFTKLEDVNLLIDQMNQLIQKLI

Sequences:

>Translated_376_residues
MKFHSTNEIKYFDYNSTHPPYHEILESCLEEYFDSFYNPSGITRFSLKNQGKIEQTRKYLSQITGGKEKQFVFSSTGTEA
NYALIQSLRVMQPTLASVIVSPFEHSSMYAALGSYGFEPILIQTHRSGVIDLNHLKTLLQTHPSPVICLYAGNETGVIQP
AEEIRSLTKQYGQLFLSDLMQAFCKIPVPFHLFDGYTFSGHKIGAGMGIAVTYFPEENKEFRLFGGGNQENEHRAGTENT
FAISCFQKVTEIQLANLEEKNVRLQTFQTLLESNLESFGCEIIAKEERRLPNTTFLILPIQTVDFFLLGLEEKGMIVSTG
SSCKSRAREASKSLLHMGYSEEEALRAIRISTGSFTKLEDVNLLIDQMNQLIQKLI
>Mature_376_residues
MKFHSTNEIKYFDYNSTHPPYHEILESCLEEYFDSFYNPSGITRFSLKNQGKIEQTRKYLSQITGGKEKQFVFSSTGTEA
NYALIQSLRVMQPTLASVIVSPFEHSSMYAALGSYGFEPILIQTHRSGVIDLNHLKTLLQTHPSPVICLYAGNETGVIQP
AEEIRSLTKQYGQLFLSDLMQAFCKIPVPFHLFDGYTFSGHKIGAGMGIAVTYFPEENKEFRLFGGGNQENEHRAGTENT
FAISCFQKVTEIQLANLEEKNVRLQTFQTLLESNLESFGCEIIAKEERRLPNTTFLILPIQTVDFFLLGLEEKGMIVSTG
SSCKSRAREASKSLLHMGYSEEEALRAIRISTGSFTKLEDVNLLIDQMNQLIQKLI

Specific function: Catalyzes the removal of elemental sulfur atoms from cysteine to produce alanine. Seems to participate in the biosynthesis of the nitrogenase metalloclusters by providing the inorganic sulfur required for the Fe-S core formation [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI156713448, Length=427, Percent_Identity=26.463700234192, Blast_Score=118, Evalue=8e-27,
Organism=Homo sapiens, GI32307132, Length=375, Percent_Identity=25.8666666666667, Blast_Score=118, Evalue=9e-27,
Organism=Escherichia coli, GI48994898, Length=378, Percent_Identity=26.4550264550265, Blast_Score=115, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI25143064, Length=367, Percent_Identity=24.7956403269755, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17533177, Length=329, Percent_Identity=28.5714285714286, Blast_Score=84, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6319831, Length=376, Percent_Identity=24.468085106383, Blast_Score=110, Evalue=3e-25,
Organism=Drosophila melanogaster, GI20129463, Length=376, Percent_Identity=26.3297872340426, Blast_Score=112, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR017772
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 42471; Mature: 42471

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFHSTNEIKYFDYNSTHPPYHEILESCLEEYFDSFYNPSGITRFSLKNQGKIEQTRKYL
CCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHH
SQITGGKEKQFVFSSTGTEANYALIQSLRVMQPTLASVIVSPFEHSSMYAALGSYGFEPI
HHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEE
LIQTHRSGVIDLNHLKTLLQTHPSPVICLYAGNETGVIQPAEEIRSLTKQYGQLFLSDLM
EEEECCCCCEEHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHH
QAFCKIPVPFHLFDGYTFSGHKIGAGMGIAVTYFPEENKEFRLFGGGNQENEHRAGTENT
HHHHCCCCCEEEECCEEECCCEECCCCEEEEEEECCCCCCEEEEECCCCCCCCCCCCCCE
FAISCFQKVTEIQLANLEEKNVRLQTFQTLLESNLESFGCEIIAKEERRLPNTTFLILPI
EEHHHHHHHHHHHHCCCCHHCHHHHHHHHHHHHCHHHHCCEEEEHHHHCCCCCEEEEEEH
QTVDFFLLGLEEKGMIVSTGSSCKSRAREASKSLLHMGYSEEEALRAIRISTGSFTKLED
HHHEEEEEEECCCCEEEECCHHHHHHHHHHHHHHHHCCCCHHHHHHEEEECCCCCCCHHH
VNLLIDQMNQLIQKLI
HHHHHHHHHHHHHHHC
>Mature Secondary Structure
MKFHSTNEIKYFDYNSTHPPYHEILESCLEEYFDSFYNPSGITRFSLKNQGKIEQTRKYL
CCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHH
SQITGGKEKQFVFSSTGTEANYALIQSLRVMQPTLASVIVSPFEHSSMYAALGSYGFEPI
HHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEE
LIQTHRSGVIDLNHLKTLLQTHPSPVICLYAGNETGVIQPAEEIRSLTKQYGQLFLSDLM
EEEECCCCCEEHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHH
QAFCKIPVPFHLFDGYTFSGHKIGAGMGIAVTYFPEENKEFRLFGGGNQENEHRAGTENT
HHHHCCCCCEEEECCEEECCCEECCCCEEEEEEECCCCCCEEEEECCCCCCCCCCCCCCE
FAISCFQKVTEIQLANLEEKNVRLQTFQTLLESNLESFGCEIIAKEERRLPNTTFLILPI
EEHHHHHHHHHHHHCCCCHHCHHHHHHHHHHHHCHHHHCCEEEEHHHHCCCCCEEEEEEH
QTVDFFLLGLEEKGMIVSTGSSCKSRAREASKSLLHMGYSEEEALRAIRISTGSFTKLED
HHHEEEEEEECCCCEEEECCHHHHHHHHHHHHHHHHCCCCHHHHHHEEEECCCCCCCHHH
VNLLIDQMNQLIQKLI
HHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7568132 [H]