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The map label for this gene is mvaB [H]

Identifier: 183221315

GI number: 183221315

Start: 2019208

End: 2020107

Strand: Direct

Name: mvaB [H]

Synonym: LEPBI_I1932

Alternate gene names: 183221315

Gene position: 2019208-2020107 (Clockwise)

Preceding gene: 183221313

Following gene: 183221316

Centisome position: 56.09

GC content: 39.67

Gene sequence:

>900_bases
TTGGAGAAAGTAAAAATCACAGAAGTAGGTCCAAGAGACGGATTACAAAATGAAAAAACCATTCTCTCCACTCAGGATAA
ATTCGAATTCATAAAACGTCTAGTAGAATCTGGTGCCAAACACATTGAACTCACCTCTTTTGTCAGAAAGGATCGGATTC
CACAAATGGCAGATGCCATGGAACTCTCCGCTTTAGTTTTACCCTTGTATGCAAAAGATGTTCAATTTTCTTGTTTAACT
CCCAATACTAAAGGGTATGAAGGAGCCAAACTTGCTGGATTCAATGAAGTGGCCGTGTTCACTGCTACCTCTGAATCGTT
TACCAAAAAAAATATCAACATGACAGTGAAAGAAAGTTTTGAATCCTTCCAACCAATCTTTGGTTTGGCAAAACAAGATG
GAATCAAAGTCCGAGGTTATATATCTACTGTTGTTGCCTGTCCCTATGAAGGAAAAATTCCACCTGAAAAAACCTTGGAA
GTCGCAGAACGTTTATTAGATGCAGGTGCCTATGAAATTTCTCTAGGAGAAACCATTGGCGTTGCCGTTCCGAATGAAAT
TGAATCTCTGCTTTCAGTTTTACTGAAAAAAATACCAATCTCTTATCTCAATTGCCATTTTCACGATACCTATGGGATGG
CGATTGCCAATACAAAACAAGCACTCTCGATGGGCATTCGTAGTTTTGATAGTTCCGCAGGAGGACTCGGAGGATGTCCT
TATGCGAAAGGGGCAGCGGGGAATGTGGCAACAGAAGATTTGGTATATTTTTTATCGAGAGAAGGGTATGATACAGGGAT
CAACTTAGATTCACTCATACAAGTGAGTCAGTTTATGGAAGCCAAATTGGACCGATCATTCAATTCGAGAACCTATATTG
CCAAAAAAAATGTTGGATGA

Upstream 100 bases:

>100_bases
GGGAAAGATTTTTGATTCTAAACATATTTTGAATTCGATAAAATCATTGCATTCTCATTTTTCCAAACAACAATGATTGG
TAGATGAAGGAGAACAAGAA

Downstream 100 bases:

>100_bases
TGGATCCCTTCTTAAAACCAAATTAGAAAATTTAATCTCCAAATACCAAAACATATCGTATTTGGAAACAGATCCCATTT
GTTTTCCCAAACAATACAAA

Product: hydroxymethylglutaryl-CoA lyase

Products: NA

Alternate protein names: HL; HMG-CoA lyase; 3-hydroxy-3-methylglutarate-CoA lyase [H]

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MEKVKITEVGPRDGLQNEKTILSTQDKFEFIKRLVESGAKHIELTSFVRKDRIPQMADAMELSALVLPLYAKDVQFSCLT
PNTKGYEGAKLAGFNEVAVFTATSESFTKKNINMTVKESFESFQPIFGLAKQDGIKVRGYISTVVACPYEGKIPPEKTLE
VAERLLDAGAYEISLGETIGVAVPNEIESLLSVLLKKIPISYLNCHFHDTYGMAIANTKQALSMGIRSFDSSAGGLGGCP
YAKGAAGNVATEDLVYFLSREGYDTGINLDSLIQVSQFMEAKLDRSFNSRTYIAKKNVG

Sequences:

>Translated_299_residues
MEKVKITEVGPRDGLQNEKTILSTQDKFEFIKRLVESGAKHIELTSFVRKDRIPQMADAMELSALVLPLYAKDVQFSCLT
PNTKGYEGAKLAGFNEVAVFTATSESFTKKNINMTVKESFESFQPIFGLAKQDGIKVRGYISTVVACPYEGKIPPEKTLE
VAERLLDAGAYEISLGETIGVAVPNEIESLLSVLLKKIPISYLNCHFHDTYGMAIANTKQALSMGIRSFDSSAGGLGGCP
YAKGAAGNVATEDLVYFLSREGYDTGINLDSLIQVSQFMEAKLDRSFNSRTYIAKKNVG
>Mature_299_residues
MEKVKITEVGPRDGLQNEKTILSTQDKFEFIKRLVESGAKHIELTSFVRKDRIPQMADAMELSALVLPLYAKDVQFSCLT
PNTKGYEGAKLAGFNEVAVFTATSESFTKKNINMTVKESFESFQPIFGLAKQDGIKVRGYISTVVACPYEGKIPPEKTLE
VAERLLDAGAYEISLGETIGVAVPNEIESLLSVLLKKIPISYLNCHFHDTYGMAIANTKQALSMGIRSFDSSAGGLGGCP
YAKGAAGNVATEDLVYFLSREGYDTGINLDSLIQVSQFMEAKLDRSFNSRTYIAKKNVG

Specific function: Involved in the catabolism of branched amino acids such as leucine (Probable) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HMG-CoA lyase family [H]

Homologues:

Organism=Homo sapiens, GI62198232, Length=291, Percent_Identity=50.1718213058419, Blast_Score=290, Evalue=1e-78,
Organism=Homo sapiens, GI109150427, Length=296, Percent_Identity=48.6486486486487, Blast_Score=287, Evalue=1e-77,
Organism=Homo sapiens, GI109150422, Length=296, Percent_Identity=48.6486486486487, Blast_Score=286, Evalue=1e-77,
Organism=Homo sapiens, GI260654708, Length=130, Percent_Identity=49.2307692307692, Blast_Score=141, Evalue=8e-34,
Organism=Escherichia coli, GI1786261, Length=302, Percent_Identity=22.5165562913907, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17510563, Length=279, Percent_Identity=45.1612903225806, Blast_Score=260, Evalue=7e-70,
Organism=Drosophila melanogaster, GI24582381, Length=294, Percent_Identity=48.6394557823129, Blast_Score=290, Evalue=1e-78,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR000138
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like [H]

EC number: =4.1.3.4 [H]

Molecular weight: Translated: 32728; Mature: 32728

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKVKITEVGPRDGLQNEKTILSTQDKFEFIKRLVESGAKHIELTSFVRKDRIPQMADAM
CCCEEEEECCCCCCCCCCCCEECCHHHHHHHHHHHHCCCCEEHHHHHHHHCCCCHHHHHH
ELSALVLPLYAKDVQFSCLTPNTKGYEGAKLAGFNEVAVFTATSESFTKKNINMTVKESF
HHHHHHHHHHHCCCEEEEECCCCCCCCCCEECCCCEEEEEEECCHHHHHCCCCCHHHHHH
ESFQPIFGLAKQDGIKVRGYISTVVACPYEGKIPPEKTLEVAERLLDAGAYEISLGETIG
HHHHHHHHHHCCCCEEEEEEEHHHEECCCCCCCCHHHHHHHHHHHHHCCCEEEECCCEEE
VAVPNEIESLLSVLLKKIPISYLNCHFHDTYGMAIANTKQALSMGIRSFDSSAGGLGGCP
CCCCHHHHHHHHHHHHHCCHHHEEEEEECCCCEEEHHHHHHHHHHHHHHCCCCCCCCCCC
YAKGAAGNVATEDLVYFLSREGYDTGINLDSLIQVSQFMEAKLDRSFNSRTYIAKKNVG
CCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCC
>Mature Secondary Structure
MEKVKITEVGPRDGLQNEKTILSTQDKFEFIKRLVESGAKHIELTSFVRKDRIPQMADAM
CCCEEEEECCCCCCCCCCCCEECCHHHHHHHHHHHHCCCCEEHHHHHHHHCCCCHHHHHH
ELSALVLPLYAKDVQFSCLTPNTKGYEGAKLAGFNEVAVFTATSESFTKKNINMTVKESF
HHHHHHHHHHHCCCEEEEECCCCCCCCCCEECCCCEEEEEEECCHHHHHCCCCCHHHHHH
ESFQPIFGLAKQDGIKVRGYISTVVACPYEGKIPPEKTLEVAERLLDAGAYEISLGETIG
HHHHHHHHHHCCCCEEEEEEEHHHEECCCCCCCCHHHHHHHHHHHHHCCCEEEECCCEEE
VAVPNEIESLLSVLLKKIPISYLNCHFHDTYGMAIANTKQALSMGIRSFDSSAGGLGGCP
CCCCHHHHHHHHHHHHHCCHHHEEEEEECCCCEEEHHHHHHHHHHHHHHCCCCCCCCCCC
YAKGAAGNVATEDLVYFLSREGYDTGINLDSLIQVSQFMEAKLDRSFNSRTYIAKKNVG
CCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2687236; 2656635; 1637819 [H]