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The map label for this gene is gshA [H]

Identifier: 183221296

GI number: 183221296

Start: 2002142

End: 2003743

Strand: Reverse

Name: gshA [H]

Synonym: LEPBI_I1913

Alternate gene names: 183221296

Gene position: 2003743-2002142 (Counterclockwise)

Preceding gene: 183221297

Following gene: 183221295

Centisome position: 55.66

GC content: 36.27

Gene sequence:

>1602_bases
ATGAAAGGAAAATTATTAAAACCGAAACAAAAAAATTCAACAGTCGCCTTATTTGCAAATGAATATAAAGACTGTCTCCT
CAGCGCCAAACATGGATTGGAGAGAGAAAGTGTACGTGTGAATGAGAAGGTATTTTTATCGCAAGAACCACATCCAAAAT
TTCTTGGATCTAGTTTGACACACCCACTCATCAAAACAGATTTTGCTGAAGCTCAAATTGAATATGCAACAAACATCCAT
AAGACGATCCCTGATGCACTTGCCGAGCTGACTGAACTACATGCTTTCACTGCCAGAAGTTTAGAAGATGAATACTTGTG
GCCATTCAGTATGCCACCAGCTTTACCAACAGAAAATAAAATTGAAGTTGGACAATACGGTACTTCTGCAGAAGGAAGGA
AAAAAACAATCTATCGCAATGGACTTGGCCATCGATATGGAAAAAAAATGCAAACGATTTCAGGGGTTCATTATAATGTG
TCCTTTGATTCTTGTATGTTGTCAGTAGTTTCAGAAAGAAGATTTCAAAAACCTCTTTCCAAAGATACAAAATCTCAAAT
CTATTTTGATACCATCCGTAATTTTTACCGAATCTCACCAGCACTCTTGTATCTTTTTGGAACATCAAGTCTAACGGATG
TCACATTTGTTGATTCAGAAAAATCGAATAAAAATTTACAAAAATTAGATGCAAAAACATTAAATGCACCGTTTGCAACT
ACCTTACGTTTATCAAGTATCGGTTATACAAGTAAGGTACAAGGAAAATATCCAATTTCTGTCAATTCTTTACATGAGTA
TGCTGCAGGGATGTGCCATGTAGTATCTAAAACCTACCCACCGTATAAACAATTTAATACAAAACCAACGAATCAATTGA
ATGATTATATCTTACAATTGGAAAATGAATACTATTCCCTCGTGAGACCAAAACAGGTTCCCAAAGGGGAAGAACGGGTC
GTGGATGCTCTTGTGGAGCGAGGTGTTGAATATTTAGAAATTAGACTTCTCGATTTAGATCCGTTTTCGGCAATTGGTGT
GGAAGAATCTCGCCTATACTTTATCCATATGGTTCTCTTGTATTGTATGTTAAATGAGTCACCTAAAGCAGATTTGGTGG
AAATGGCCAATTGGAGAAAGAACCAAGAACAAACAACTTGGTTTGGTCGAAAACCTGAAACCAAAGTGTATTTATTTGAG
AAAGAAATTTTACTACGAGATATGGTTTACCAGTTGTTTGTCGATTTGCAACCCATTGCGGATTTATTAGATGAGAATGA
TGCAAATGGACCTTTTAGCCGTGCATTGGAATCTCAATGGGAAAAATGGGACGATCCAAGTTTACTTGGTGCCACAATGT
CTGAATTGGATCTAAAAATCAACAAATCGAGTTATCGAGAATTTGGATTGGCCCTTGCCCGTTCCCATAAAGAAGAACTT
TTGCAAAAGGTCTTATCTCCCAATCGAATCAAATACTATGAAGATTTGAGTGAACAATCCATCTATGAACAAAAAAAAAT
GGAAACATTAGAAGGTAGCCATTTGAAAAAAAATCAAAAGCCCATCCAAATCAAACCTCTCAAACTTTGTAGTGAGGTTT
AA

Upstream 100 bases:

>100_bases
TAGGCACCGAGCAAAAGTATTCGTAGTGTCAAAAGAAGGAGACAAACTGATAGGAGTGTCGGATCCGAAAGGGGAAGGAG
TTCCTTTAGGTTTTTAGTCG

Downstream 100 bases:

>100_bases
AATGACAGAACCGAAACCACTACTTCCTGGTTGGGAAGACTTGGAGATTTCGACCCAGATCATCATTCGAGATGCGATTT
CACGAGGGATTGAGGTGCAG

Product: glutamate--cysteine ligase

Products: NA

Alternate protein names: Gamma-ECS; GCS; Gamma-glutamylcysteine synthetase [H]

Number of amino acids: Translated: 533; Mature: 533

Protein sequence:

>533_residues
MKGKLLKPKQKNSTVALFANEYKDCLLSAKHGLERESVRVNEKVFLSQEPHPKFLGSSLTHPLIKTDFAEAQIEYATNIH
KTIPDALAELTELHAFTARSLEDEYLWPFSMPPALPTENKIEVGQYGTSAEGRKKTIYRNGLGHRYGKKMQTISGVHYNV
SFDSCMLSVVSERRFQKPLSKDTKSQIYFDTIRNFYRISPALLYLFGTSSLTDVTFVDSEKSNKNLQKLDAKTLNAPFAT
TLRLSSIGYTSKVQGKYPISVNSLHEYAAGMCHVVSKTYPPYKQFNTKPTNQLNDYILQLENEYYSLVRPKQVPKGEERV
VDALVERGVEYLEIRLLDLDPFSAIGVEESRLYFIHMVLLYCMLNESPKADLVEMANWRKNQEQTTWFGRKPETKVYLFE
KEILLRDMVYQLFVDLQPIADLLDENDANGPFSRALESQWEKWDDPSLLGATMSELDLKINKSSYREFGLALARSHKEEL
LQKVLSPNRIKYYEDLSEQSIYEQKKMETLEGSHLKKNQKPIQIKPLKLCSEV

Sequences:

>Translated_533_residues
MKGKLLKPKQKNSTVALFANEYKDCLLSAKHGLERESVRVNEKVFLSQEPHPKFLGSSLTHPLIKTDFAEAQIEYATNIH
KTIPDALAELTELHAFTARSLEDEYLWPFSMPPALPTENKIEVGQYGTSAEGRKKTIYRNGLGHRYGKKMQTISGVHYNV
SFDSCMLSVVSERRFQKPLSKDTKSQIYFDTIRNFYRISPALLYLFGTSSLTDVTFVDSEKSNKNLQKLDAKTLNAPFAT
TLRLSSIGYTSKVQGKYPISVNSLHEYAAGMCHVVSKTYPPYKQFNTKPTNQLNDYILQLENEYYSLVRPKQVPKGEERV
VDALVERGVEYLEIRLLDLDPFSAIGVEESRLYFIHMVLLYCMLNESPKADLVEMANWRKNQEQTTWFGRKPETKVYLFE
KEILLRDMVYQLFVDLQPIADLLDENDANGPFSRALESQWEKWDDPSLLGATMSELDLKINKSSYREFGLALARSHKEEL
LQKVLSPNRIKYYEDLSEQSIYEQKKMETLEGSHLKKNQKPIQIKPLKLCSEV
>Mature_533_residues
MKGKLLKPKQKNSTVALFANEYKDCLLSAKHGLERESVRVNEKVFLSQEPHPKFLGSSLTHPLIKTDFAEAQIEYATNIH
KTIPDALAELTELHAFTARSLEDEYLWPFSMPPALPTENKIEVGQYGTSAEGRKKTIYRNGLGHRYGKKMQTISGVHYNV
SFDSCMLSVVSERRFQKPLSKDTKSQIYFDTIRNFYRISPALLYLFGTSSLTDVTFVDSEKSNKNLQKLDAKTLNAPFAT
TLRLSSIGYTSKVQGKYPISVNSLHEYAAGMCHVVSKTYPPYKQFNTKPTNQLNDYILQLENEYYSLVRPKQVPKGEERV
VDALVERGVEYLEIRLLDLDPFSAIGVEESRLYFIHMVLLYCMLNESPKADLVEMANWRKNQEQTTWFGRKPETKVYLFE
KEILLRDMVYQLFVDLQPIADLLDENDANGPFSRALESQWEKWDDPSLLGATMSELDLKINKSSYREFGLALARSHKEEL
LQKVLSPNRIKYYEDLSEQSIYEQKKMETLEGSHLKKNQKPIQIKPLKLCSEV

Specific function: Glutathione biosynthesis; first step. [C]

COG id: COG2918

COG function: function code H; Gamma-glutamylcysteine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate--cysteine ligase type 1 family. Type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789044, Length=494, Percent_Identity=35.0202429149798, Blast_Score=289, Evalue=3e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007370
- InterPro:   IPR006334 [H]

Pfam domain/function: PF04262 Glu_cys_ligase [H]

EC number: =6.3.2.2 [H]

Molecular weight: Translated: 61329; Mature: 61329

Theoretical pI: Translated: 8.16; Mature: 8.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGKLLKPKQKNSTVALFANEYKDCLLSAKHGLERESVRVNEKVFLSQEPHPKFLGSSLT
CCCCCCCCCCCCCEEEEEHHHHHHHHHHHHCCCCHHHEECCCEEEECCCCCHHHHCCHHC
HPLIKTDFAEAQIEYATNIHKTIPDALAELTELHAFTARSLEDEYLWPFSMPPALPTENK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
IEVGQYGTSAEGRKKTIYRNGLGHRYGKKMQTISGVHYNVSFDSCMLSVVSERRFQKPLS
EEECCCCCCCCCCHHHHHHCCCCHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHHCCCC
KDTKSQIYFDTIRNFYRISPALLYLFGTSSLTDVTFVDSEKSNKNLQKLDAKTLNAPFAT
CCHHHHHHHHHHHHHHHHCHHHEEEECCCCCCEEEEECCCCCCCHHHHHHHHHCCCCCHH
TLRLSSIGYTSKVQGKYPISVNSLHEYAAGMCHVVSKTYPPYKQFNTKPTNQLNDYILQL
EEEEHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCHHHHHHHHHH
ENEYYSLVRPKQVPKGEERVVDALVERGVEYLEIRLLDLDPFSAIGVEESRLYFIHMVLL
HHHHHHHCCCCCCCCHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHH
YCMLNESPKADLVEMANWRKNQEQTTWFGRKPETKVYLFEKEILLRDMVYQLFVDLQPIA
HHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHH
DLLDENDANGPFSRALESQWEKWDDPSLLGATMSELDLKINKSSYREFGLALARSHKEEL
HHHCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHEEECHHHHHHHHHHHHHHHHHHH
LQKVLSPNRIKYYEDLSEQSIYEQKKMETLEGSHLKKNQKPIQIKPLKLCSEV
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCEEEHHHHHCCC
>Mature Secondary Structure
MKGKLLKPKQKNSTVALFANEYKDCLLSAKHGLERESVRVNEKVFLSQEPHPKFLGSSLT
CCCCCCCCCCCCCEEEEEHHHHHHHHHHHHCCCCHHHEECCCEEEECCCCCHHHHCCHHC
HPLIKTDFAEAQIEYATNIHKTIPDALAELTELHAFTARSLEDEYLWPFSMPPALPTENK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
IEVGQYGTSAEGRKKTIYRNGLGHRYGKKMQTISGVHYNVSFDSCMLSVVSERRFQKPLS
EEECCCCCCCCCCHHHHHHCCCCHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHHCCCC
KDTKSQIYFDTIRNFYRISPALLYLFGTSSLTDVTFVDSEKSNKNLQKLDAKTLNAPFAT
CCHHHHHHHHHHHHHHHHCHHHEEEECCCCCCEEEEECCCCCCCHHHHHHHHHCCCCCHH
TLRLSSIGYTSKVQGKYPISVNSLHEYAAGMCHVVSKTYPPYKQFNTKPTNQLNDYILQL
EEEEHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCHHHHHHHHHH
ENEYYSLVRPKQVPKGEERVVDALVERGVEYLEIRLLDLDPFSAIGVEESRLYFIHMVLL
HHHHHHHCCCCCCCCHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHH
YCMLNESPKADLVEMANWRKNQEQTTWFGRKPETKVYLFEKEILLRDMVYQLFVDLQPIA
HHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHH
DLLDENDANGPFSRALESQWEKWDDPSLLGATMSELDLKINKSSYREFGLALARSHKEEL
HHHCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHEEECHHHHHHHHHHHHHHHHHHH
LQKVLSPNRIKYYEDLSEQSIYEQKKMETLEGSHLKKNQKPIQIKPLKLCSEV
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCEEEHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA