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The map label for this gene is gshB [H]

Identifier: 183221295

GI number: 183221295

Start: 2001133

End: 2002140

Strand: Reverse

Name: gshB [H]

Synonym: LEPBI_I1912

Alternate gene names: 183221295

Gene position: 2002140-2001133 (Counterclockwise)

Preceding gene: 183221296

Following gene: 183221281

Centisome position: 55.62

GC content: 40.08

Gene sequence:

>1008_bases
ATGACAGAACCGAAACCACTACTTCCTGGTTGGGAAGACTTGGAGATTTCGACCCAGATCATCATTCGAGATGCGATTTC
ACGAGGGATTGAGGTGCAGGTGATCGATCGGAAAGAAAATTTCCTAAGATTGAAACAAGGCAGCCATACCGAATTTGTAA
AGGAAGCCAGTAAAACAAGACTCGATAGTCTCATGACCTATCTCGTGATGGAAAATAAAATCGCCTCCAAACTAGTGTTA
GCAGAGGATGGGGTTCGTGTTCCGATCGGAAAACATTATTCTAGACTTGAAGATGCCCTAAACGATTATACCATTTTTCA
AAACAAAAAAAAAGTTGTAAAACCTGTGACAACCAATTTTGGAATTGGAATCGGAATATCAGAACCTAATGATTCCCTAG
AAAAATTCACATATTTTGTAAAACAATCTTTTTCCTTTTCGAGTTCCATCATCATTGAAGAGTTCATTGAGGGACCGGAG
TATCGATTTTTAGTATTAGGTGATGAGGTAGTGGCTGTATGCAACCGAGTCCCGGCTAATGTCGTCGGAGATGGAATCAA
AACCATATCAGAATTGATCGAAGAAAAAAATAGGGACCCTAGAAGGGGGGAGGGACATATCACGGCGTTGGAAAAAATAC
AAATGTCAGATGTGGAACGACTGGTTCTTTTGGACCAAGGATTTGAATTTTTATCCATTCCGAAAAAAGGGGAACAAGTT
TTTTTACGGAAAAATTCCAATATTTCTACGGGTGGAGATTCTGTGGACGTCACAGATCTTGTTCATCCCGAATTCAAAAC
CATTGCATTGAAAGCAGCAAGAGCTGCGGGAGCTGTGATTTGTGGGATTGATATCATATCAAGTGCCATCACAGAAACAC
CAAATCCTGAAACCTACGCGATCTTAGAAATTAATTTCAATCCAGTGTTGTACATCCATGAATTTCCATATTCGGGAACA
CCAAGGGCCGTAGGAGATACGATTTTAAATTTACTAGGGTTTCATTAG

Upstream 100 bases:

>100_bases
TATGAACAAAAAAAAATGGAAACATTAGAAGGTAGCCATTTGAAAAAAAATCAAAAGCCCATCCAAATCAAACCTCTCAA
ACTTTGTAGTGAGGTTTAAA

Downstream 100 bases:

>100_bases
AAGAGTGAGTGGACCGTTTTTTCCTTCAAACCGAAGATTGAGAATGCGGATTTCGGAGATTTGAGTAAAACCAAGAACTA
CTAACAGTTAGATGGATTGC

Product: glutathione synthase

Products: NA

Alternate protein names: Gamma-GCS-GS; GCS-GS; Glutamate--cysteine ligase; Gamma-ECS; GCS; Gamma-glutamylcysteine synthetase; Glutathione synthetase; GSH synthetase; GS; GSH-S; GSHase; Glutathione synthase [H]

Number of amino acids: Translated: 335; Mature: 334

Protein sequence:

>335_residues
MTEPKPLLPGWEDLEISTQIIIRDAISRGIEVQVIDRKENFLRLKQGSHTEFVKEASKTRLDSLMTYLVMENKIASKLVL
AEDGVRVPIGKHYSRLEDALNDYTIFQNKKKVVKPVTTNFGIGIGISEPNDSLEKFTYFVKQSFSFSSSIIIEEFIEGPE
YRFLVLGDEVVAVCNRVPANVVGDGIKTISELIEEKNRDPRRGEGHITALEKIQMSDVERLVLLDQGFEFLSIPKKGEQV
FLRKNSNISTGGDSVDVTDLVHPEFKTIALKAARAAGAVICGIDIISSAITETPNPETYAILEINFNPVLYIHEFPYSGT
PRAVGDTILNLLGFH

Sequences:

>Translated_335_residues
MTEPKPLLPGWEDLEISTQIIIRDAISRGIEVQVIDRKENFLRLKQGSHTEFVKEASKTRLDSLMTYLVMENKIASKLVL
AEDGVRVPIGKHYSRLEDALNDYTIFQNKKKVVKPVTTNFGIGIGISEPNDSLEKFTYFVKQSFSFSSSIIIEEFIEGPE
YRFLVLGDEVVAVCNRVPANVVGDGIKTISELIEEKNRDPRRGEGHITALEKIQMSDVERLVLLDQGFEFLSIPKKGEQV
FLRKNSNISTGGDSVDVTDLVHPEFKTIALKAARAAGAVICGIDIISSAITETPNPETYAILEINFNPVLYIHEFPYSGT
PRAVGDTILNLLGFH
>Mature_334_residues
TEPKPLLPGWEDLEISTQIIIRDAISRGIEVQVIDRKENFLRLKQGSHTEFVKEASKTRLDSLMTYLVMENKIASKLVLA
EDGVRVPIGKHYSRLEDALNDYTIFQNKKKVVKPVTTNFGIGIGISEPNDSLEKFTYFVKQSFSFSSSIIIEEFIEGPEY
RFLVLGDEVVAVCNRVPANVVGDGIKTISELIEEKNRDPRRGEGHITALEKIQMSDVERLVLLDQGFEFLSIPKKGEQVF
LRKNSNISTGGDSVDVTDLVHPEFKTIALKAARAAGAVICGIDIISSAITETPNPETYAILEINFNPVLYIHEFPYSGTP
RAVGDTILNLLGFH

Specific function: Synthesizes glutathione from L-glutamate and L-cysteine via gamma-L-glutamyl-L-cysteine [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR007370
- InterPro:   IPR006335
- InterPro:   IPR020561 [H]

Pfam domain/function: PF01071 GARS_A; PF04262 Glu_cys_ligase [H]

EC number: =6.3.2.2; =6.3.2.3 [H]

Molecular weight: Translated: 37370; Mature: 37239

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEPKPLLPGWEDLEISTQIIIRDAISRGIEVQVIDRKENFLRLKQGSHTEFVKEASKTR
CCCCCCCCCCCCCCCHHHHHHEEHHHHCCCEEEEEECCCCEEEECCCCCHHHHHHHHHHH
LDSLMTYLVMENKIASKLVLAEDGVRVPIGKHYSRLEDALNDYTIFQNKKKVVKPVTTNF
HHHHHHHHHHHHHHHHEEEEECCCEECCCCHHHHHHHHHHHHCEECCCCCHHHCEEECCC
GIGIGISEPNDSLEKFTYFVKQSFSFSSSIIIEEFIEGPEYRFLVLGDEVVAVCNRVPAN
CEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEEECHHHHHHHHCCCCH
VVGDGIKTISELIEEKNRDPRRGEGHITALEKIQMSDVERLVLLDQGFEFLSIPKKGEQV
HHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHEEHHHCCCCEEECCCCCCEE
FLRKNSNISTGGDSVDVTDLVHPEFKTIALKAARAAGAVICGIDIISSAITETPNPETYA
EEEECCCCCCCCCCEEHHHHCCCCHHHHHHHHHHHCCEEEEHHHHHHHHHHCCCCCCEEE
ILEINFNPVLYIHEFPYSGTPRAVGDTILNLLGFH
EEEECCCEEEEEEECCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
TEPKPLLPGWEDLEISTQIIIRDAISRGIEVQVIDRKENFLRLKQGSHTEFVKEASKTR
CCCCCCCCCCCCCCHHHHHHEEHHHHCCCEEEEEECCCCEEEECCCCCHHHHHHHHHHH
LDSLMTYLVMENKIASKLVLAEDGVRVPIGKHYSRLEDALNDYTIFQNKKKVVKPVTTNF
HHHHHHHHHHHHHHHHEEEEECCCEECCCCHHHHHHHHHHHHCEECCCCCHHHCEEECCC
GIGIGISEPNDSLEKFTYFVKQSFSFSSSIIIEEFIEGPEYRFLVLGDEVVAVCNRVPAN
CEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEEECHHHHHHHHCCCCH
VVGDGIKTISELIEEKNRDPRRGEGHITALEKIQMSDVERLVLLDQGFEFLSIPKKGEQV
HHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHEEHHHCCCCEEECCCCCCEE
FLRKNSNISTGGDSVDVTDLVHPEFKTIALKAARAAGAVICGIDIISSAITETPNPETYA
EEEECCCCCCCCCCEEHHHHCCCCHHHHHHHHHHHCCEEEEHHHHHHHHHHCCCCCCEEE
ILEINFNPVLYIHEFPYSGTPRAVGDTILNLLGFH
EEEECCCEEEEEEECCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11792842 [H]