Click here to switch to the map view.

The map label for this gene is lysS [H]

Identifier: 183221280

GI number: 183221280

Start: 1989515

End: 1991008

Strand: Direct

Name: lysS [H]

Synonym: LEPBI_I1897

Alternate gene names: 183221280

Gene position: 1989515-1991008 (Clockwise)

Preceding gene: 183221279

Following gene: 183221282

Centisome position: 55.27

GC content: 42.24

Gene sequence:

>1494_bases
ATGGATGAAATTAAAGATTCAAACGAACTCATCGAACAACGAATTCAAAAAATTCAAGACTTAAAAACAAAAGGAATCAA
CCCCTACCCACTTCGTTTTTTTCCCAATTCCAATTCAAAATTTTTGTTGGAAAATTTTGATCCAAACAACACTGAAAAAA
AATCTTTCAAACTCGGCGGACGTTTGCATGCAAAACGTGTGATGGGAAAGGCAAGTTTTGCCCACCTCAAAGATGCGGAA
GGCCTCATCCAATTGTATGCCACACGTGATGACTTGGGTGAAGAAAATTATTCTCTATTCAAATCCCTTGACCTTGGGGA
TTGGATTGGAATCGAGGGATGGTTATTCCAAACACAAAAAGGTGAAACCACTCTTCATTTAACGAATGTTCAACTACTTG
CAAAATGCATTCGACCGCTACCAGTCGTGAAGGAAAAAGACGGTGTGATTTATGATGCCTTCTCTGATGTAGAACAAAGG
TATCGAATGCGGTATGTGGACCTTGTGGTCAATGAAAACGTCCGCGAAACGTTTAAAATGCGTTCTCGTATCATTTCAGA
AATTCGAAAGTTTCTTACAAATGAAGGGTTTTTGGAAGTGGAAACTCCTATGATGCAACCCATTGCAGGTGGTGCAGCGG
CAAGACCTTTTGTTACCCACCACAATACTTTGGATATGGAACTCTTTTTACGGATTGCACCCGAACTCTATTTAAAACGA
CTCATTGTGGGTGGAATGGACCGAGTGTTTGAATTGAACCGTAACTTTCGGAATGAAGGGATCTCCACCAAACACAATCC
TGAATTTACAATGATGGAAGCCTATATGGCCTTTGGCGATATGGAAGCCATGCTTTCTCTCACAGAAAGGATGATCGTTT
CTGTTGCCACTGCCATTGGAAAAGGTTTAAAATTTCCTTATGGAAAGGACCAAATTGACCTGACGCCACCTTGGAAACGA
GTGAAGTACATCGACATCATCAAAGACTACTCGGGAATTGATTTTAGCCAAATTACCGATGTCAAAGAAGCCATAGAAAA
AGCCAAAGCGAAAGGAGTGGATTCTTCTGATTCCGTTTCGATATGGAAGGTATGTGACGATGTGTTTAGTTCGCTTGTGG
AACCACACCTCATCCAACCCATCTTTATCACAGACTTTCCGAAAGAACTCTCTCCCCTTGCCAAATCAAGAGAAGATGAT
CCAAAGTATGTAGAACGTTTTGAACCCTATGTGGCAGGAAGAGAGATTGGGAACGCCTTCACTGAGTTAAACGATCCTTT
TGACCAAAGGGAACGTTTTGAAGAACAGGTGAAACAAAGGGAAGCGGGCGACGATGAAGCCTTCATGATGGATGATGACT
ACATCCGAGCTTTGGAATATGGACTTCCGCCAACGGGTGGCCTAGGGATTGGAATTGATCGACTTGTGATGTTACTCACA
GACTCCCATTCCATTCGGGATACCATCCTATTCCCACTCATGCGTCCAGAATAG

Upstream 100 bases:

>100_bases
GTTTCTGCCTCTAATTGGTTTTTATCAGAATCAGGCGTGAAAGAAATTTCTGTTGAATTAGTACGTTATATTTATTCTTA
CACTAGAGGTATCCTTTAAA

Downstream 100 bases:

>100_bases
AACCGAACATTTTCGTATAAAAAAACCTCTCCGAACCAATGGGTGCCGGAGAGGTGATTGTTTCGGGAAACGTTTGATTT
CCCGAACGATTCCAAAACGA

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS [H]

Number of amino acids: Translated: 497; Mature: 497

Protein sequence:

>497_residues
MDEIKDSNELIEQRIQKIQDLKTKGINPYPLRFFPNSNSKFLLENFDPNNTEKKSFKLGGRLHAKRVMGKASFAHLKDAE
GLIQLYATRDDLGEENYSLFKSLDLGDWIGIEGWLFQTQKGETTLHLTNVQLLAKCIRPLPVVKEKDGVIYDAFSDVEQR
YRMRYVDLVVNENVRETFKMRSRIISEIRKFLTNEGFLEVETPMMQPIAGGAAARPFVTHHNTLDMELFLRIAPELYLKR
LIVGGMDRVFELNRNFRNEGISTKHNPEFTMMEAYMAFGDMEAMLSLTERMIVSVATAIGKGLKFPYGKDQIDLTPPWKR
VKYIDIIKDYSGIDFSQITDVKEAIEKAKAKGVDSSDSVSIWKVCDDVFSSLVEPHLIQPIFITDFPKELSPLAKSREDD
PKYVERFEPYVAGREIGNAFTELNDPFDQRERFEEQVKQREAGDDEAFMMDDDYIRALEYGLPPTGGLGIGIDRLVMLLT
DSHSIRDTILFPLMRPE

Sequences:

>Translated_497_residues
MDEIKDSNELIEQRIQKIQDLKTKGINPYPLRFFPNSNSKFLLENFDPNNTEKKSFKLGGRLHAKRVMGKASFAHLKDAE
GLIQLYATRDDLGEENYSLFKSLDLGDWIGIEGWLFQTQKGETTLHLTNVQLLAKCIRPLPVVKEKDGVIYDAFSDVEQR
YRMRYVDLVVNENVRETFKMRSRIISEIRKFLTNEGFLEVETPMMQPIAGGAAARPFVTHHNTLDMELFLRIAPELYLKR
LIVGGMDRVFELNRNFRNEGISTKHNPEFTMMEAYMAFGDMEAMLSLTERMIVSVATAIGKGLKFPYGKDQIDLTPPWKR
VKYIDIIKDYSGIDFSQITDVKEAIEKAKAKGVDSSDSVSIWKVCDDVFSSLVEPHLIQPIFITDFPKELSPLAKSREDD
PKYVERFEPYVAGREIGNAFTELNDPFDQRERFEEQVKQREAGDDEAFMMDDDYIRALEYGLPPTGGLGIGIDRLVMLLT
DSHSIRDTILFPLMRPE
>Mature_497_residues
MDEIKDSNELIEQRIQKIQDLKTKGINPYPLRFFPNSNSKFLLENFDPNNTEKKSFKLGGRLHAKRVMGKASFAHLKDAE
GLIQLYATRDDLGEENYSLFKSLDLGDWIGIEGWLFQTQKGETTLHLTNVQLLAKCIRPLPVVKEKDGVIYDAFSDVEQR
YRMRYVDLVVNENVRETFKMRSRIISEIRKFLTNEGFLEVETPMMQPIAGGAAARPFVTHHNTLDMELFLRIAPELYLKR
LIVGGMDRVFELNRNFRNEGISTKHNPEFTMMEAYMAFGDMEAMLSLTERMIVSVATAIGKGLKFPYGKDQIDLTPPWKR
VKYIDIIKDYSGIDFSQITDVKEAIEKAKAKGVDSSDSVSIWKVCDDVFSSLVEPHLIQPIFITDFPKELSPLAKSREDD
PKYVERFEPYVAGREIGNAFTELNDPFDQRERFEEQVKQREAGDDEAFMMDDDYIRALEYGLPPTGGLGIGIDRLVMLLT
DSHSIRDTILFPLMRPE

Specific function: Also Can Synthesize A Number Of Adenyl Dinucleotides (In Particular Appppa). These Dinucleotides Have Been Proposed To Act As Modulators Of The Heat-Shock Response And Stress Response. [C]

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI5031815, Length=517, Percent_Identity=40.8123791102514, Blast_Score=383, Evalue=1e-106,
Organism=Homo sapiens, GI194272210, Length=517, Percent_Identity=40.8123791102514, Blast_Score=382, Evalue=1e-106,
Organism=Homo sapiens, GI45439306, Length=457, Percent_Identity=22.7571115973742, Blast_Score=92, Evalue=8e-19,
Organism=Homo sapiens, GI40789249, Length=332, Percent_Identity=21.9879518072289, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI1790571, Length=503, Percent_Identity=48.7077534791253, Blast_Score=486, Evalue=1e-138,
Organism=Escherichia coli, GI1789256, Length=502, Percent_Identity=48.605577689243, Blast_Score=483, Evalue=1e-138,
Organism=Escherichia coli, GI87082379, Length=322, Percent_Identity=32.2981366459627, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI1788173, Length=293, Percent_Identity=23.8907849829352, Blast_Score=76, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17535925, Length=512, Percent_Identity=40.4296875, Blast_Score=375, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI17535927, Length=512, Percent_Identity=40.4296875, Blast_Score=375, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI71994340, Length=504, Percent_Identity=40.6746031746032, Blast_Score=372, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI32566633, Length=333, Percent_Identity=26.1261261261261, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17551876, Length=473, Percent_Identity=23.4672304439746, Blast_Score=80, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6320242, Length=522, Percent_Identity=40.9961685823755, Blast_Score=381, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6324256, Length=480, Percent_Identity=29.7916666666667, Blast_Score=181, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6323011, Length=461, Percent_Identity=24.9457700650759, Blast_Score=97, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6325153, Length=250, Percent_Identity=28.4, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24640849, Length=512, Percent_Identity=41.40625, Blast_Score=388, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24640851, Length=512, Percent_Identity=41.40625, Blast_Score=387, Evalue=1e-108,
Organism=Drosophila melanogaster, GI17136276, Length=454, Percent_Identity=23.7885462555066, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24584738, Length=295, Percent_Identity=26.1016949152542, Blast_Score=80, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.6 [H]

Molecular weight: Translated: 57089; Mature: 57089

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDEIKDSNELIEQRIQKIQDLKTKGINPYPLRFFPNSNSKFLLENFDPNNTEKKSFKLGG
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCEEEEECCCCCCCCHHHHHCCC
RLHAKRVMGKASFAHLKDAEGLIQLYATRDDLGEENYSLFKSLDLGDWIGIEGWLFQTQK
HHHHHHHHHHHHHHHHHHHHHHHHHEECHHHCCCCHHHHHHHCCCCCCCCCCCEEEEECC
GETTLHLTNVQLLAKCIRPLPVVKEKDGVIYDAFSDVEQRYRMRYVDLVVNENVRETFKM
CCEEEEEHHHHHHHHHHCCCCCEECCCCEEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
RSRIISEIRKFLTNEGFLEVETPMMQPIAGGAAARPFVTHHNTLDMELFLRIAPELYLKR
HHHHHHHHHHHHCCCCCEEECCCHHHHCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHH
LIVGGMDRVFELNRNFRNEGISTKHNPEFTMMEAYMAFGDMEAMLSLTERMIVSVATAIG
HHHCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KGLKFPYGKDQIDLTPPWKRVKYIDIIKDYSGIDFSQITDVKEAIEKAKAKGVDSSDSVS
CCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCH
IWKVCDDVFSSLVEPHLIQPIFITDFPKELSPLAKSREDDPKYVERFEPYVAGREIGNAF
HHHHHHHHHHHHCCHHHCCCEEECCCCHHHHHHHHCCCCCHHHHHHHCHHHHHHHHHHHH
TELNDPFDQRERFEEQVKQREAGDDEAFMMDDDYIRALEYGLPPTGGLGIGIDRLVMLLT
HHCCCCHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHCCCCCCCCCCCHHHHHHHHC
DSHSIRDTILFPLMRPE
CCCCHHHHHCCCCCCCC
>Mature Secondary Structure
MDEIKDSNELIEQRIQKIQDLKTKGINPYPLRFFPNSNSKFLLENFDPNNTEKKSFKLGG
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCEEEEECCCCCCCCHHHHHCCC
RLHAKRVMGKASFAHLKDAEGLIQLYATRDDLGEENYSLFKSLDLGDWIGIEGWLFQTQK
HHHHHHHHHHHHHHHHHHHHHHHHHEECHHHCCCCHHHHHHHCCCCCCCCCCCEEEEECC
GETTLHLTNVQLLAKCIRPLPVVKEKDGVIYDAFSDVEQRYRMRYVDLVVNENVRETFKM
CCEEEEEHHHHHHHHHHCCCCCEECCCCEEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
RSRIISEIRKFLTNEGFLEVETPMMQPIAGGAAARPFVTHHNTLDMELFLRIAPELYLKR
HHHHHHHHHHHHCCCCCEEECCCHHHHCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHH
LIVGGMDRVFELNRNFRNEGISTKHNPEFTMMEAYMAFGDMEAMLSLTERMIVSVATAIG
HHHCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KGLKFPYGKDQIDLTPPWKRVKYIDIIKDYSGIDFSQITDVKEAIEKAKAKGVDSSDSVS
CCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCH
IWKVCDDVFSSLVEPHLIQPIFITDFPKELSPLAKSREDDPKYVERFEPYVAGREIGNAF
HHHHHHHHHHHHCCHHHCCCEEECCCCHHHHHHHHCCCCCHHHHHHHCHHHHHHHHHHHH
TELNDPFDQRERFEEQVKQREAGDDEAFMMDDDYIRALEYGLPPTGGLGIGIDRLVMLLT
HHCCCCHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHCCCCCCCCCCCHHHHHHHHC
DSHSIRDTILFPLMRPE
CCCCHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA