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The map label for this gene is mraW

Identifier: 183221148

GI number: 183221148

Start: 1840510

End: 1841454

Strand: Reverse

Name: mraW

Synonym: LEPBI_I1762

Alternate gene names: 183221148

Gene position: 1841454-1840510 (Counterclockwise)

Preceding gene: 183221150

Following gene: 183221147

Centisome position: 51.16

GC content: 41.69

Gene sequence:

>945_bases
GTGTTAGAATCTCCTCACATACCCGTCCTGCCAAGTGAAGTCATCTCCTTATTACAACAGACCAAAAATCCGAATCCGCA
ATGGTTTTTGGATGGGACCGCTGGAGAAGGTGGACACTCCAAACTCATTCTAAAAACCTTCCCCGAGGCAAAACTCATCC
TCATCGATCGAGATGCAGTGATGTTAGAAAGAGCAAAAAAAGAGATCTTAAAAGAAATAGGTTCTCTTGACCGGGTCCAT
GCCTTCCAAATGAACTTTTCAGAAGTAGATTCTGAGTTATTACAAGAAGTAGGTTGTCCTTCGCTTGATGGGGCACTTGT
GGATCTCGGCGTTTCCCTTTTCCATTTTTTGCATTCGGGTAGGGGATTCACGTTTAAGAATGATGAGCCGCTTGATATGC
GGTTGGAAGCCAATATCGGTGGAAAAACAGCTGCCGATGTAGTGAATTATAGTTCAGTTTTGCACTTAAAAAAAGTATTT
TGGGAGTATGGGGAGGAACGTTGGGCCTTAAAAATTGCAAATAACATCGTACAGACGAGACACAAAAAGAAATTTGGAAC
CAATACCGATTTAGTAAAACTTGTAGAAGCATCCATCCCAAGAAAGTTTTGGCCAAAAGAATCGCATCCTGCCACAAGGA
TCTTCCAAGCCCTACGAATTGAAGTGAATGAAGAATTGGTCCATGCGGAAAAAGGCATTCGAGCACTTGCCCAATGTTTG
GGAATAGGTGGTGTCCTCACTTGTATTTCCTTCCATTCATTGGAAGACCGGATTGTCAAATGGACCTTTCGGGATTTGAA
AGACAATGGACCATTCGAAATTTTAACCAAAAAACCCATATTACCTTCCGAGACAGAAATCAAGGAAAACAGAGCCTCAC
GATCGGCAAAATTAAGAGGTCTTATGAAAATCAATCCGATAAAAGAAAGTAGATGGGAAAAATGA

Upstream 100 bases:

>100_bases
GTATGGAATGTTCTTCATAGGAACTCATTGATACTTTCAATCTCCGAGAAGGATTAAAGGATTGCAAGGATTTTGACAAG
TATACAGTAAGAAAGTAACA

Downstream 100 bases:

>100_bases
CACACTCAATCAAATCGATACCCAATCTGGTGTCCGATTTTTTGGCACCTCTTCAATCACTATTGGTGCTTTTTCCCCTC
TTAGGATTTTTTTTCTTTTT

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MLESPHIPVLPSEVISLLQQTKNPNPQWFLDGTAGEGGHSKLILKTFPEAKLILIDRDAVMLERAKKEILKEIGSLDRVH
AFQMNFSEVDSELLQEVGCPSLDGALVDLGVSLFHFLHSGRGFTFKNDEPLDMRLEANIGGKTAADVVNYSSVLHLKKVF
WEYGEERWALKIANNIVQTRHKKKFGTNTDLVKLVEASIPRKFWPKESHPATRIFQALRIEVNEELVHAEKGIRALAQCL
GIGGVLTCISFHSLEDRIVKWTFRDLKDNGPFEILTKKPILPSETEIKENRASRSAKLRGLMKINPIKESRWEK

Sequences:

>Translated_314_residues
MLESPHIPVLPSEVISLLQQTKNPNPQWFLDGTAGEGGHSKLILKTFPEAKLILIDRDAVMLERAKKEILKEIGSLDRVH
AFQMNFSEVDSELLQEVGCPSLDGALVDLGVSLFHFLHSGRGFTFKNDEPLDMRLEANIGGKTAADVVNYSSVLHLKKVF
WEYGEERWALKIANNIVQTRHKKKFGTNTDLVKLVEASIPRKFWPKESHPATRIFQALRIEVNEELVHAEKGIRALAQCL
GIGGVLTCISFHSLEDRIVKWTFRDLKDNGPFEILTKKPILPSETEIKENRASRSAKLRGLMKINPIKESRWEK
>Mature_314_residues
MLESPHIPVLPSEVISLLQQTKNPNPQWFLDGTAGEGGHSKLILKTFPEAKLILIDRDAVMLERAKKEILKEIGSLDRVH
AFQMNFSEVDSELLQEVGCPSLDGALVDLGVSLFHFLHSGRGFTFKNDEPLDMRLEANIGGKTAADVVNYSSVLHLKKVF
WEYGEERWALKIANNIVQTRHKKKFGTNTDLVKLVEASIPRKFWPKESHPATRIFQALRIEVNEELVHAEKGIRALAQCL
GIGGVLTCISFHSLEDRIVKWTFRDLKDNGPFEILTKKPILPSETEIKENRASRSAKLRGLMKINPIKESRWEK

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=345, Percent_Identity=33.6231884057971, Blast_Score=152, Evalue=4e-37,
Organism=Homo sapiens, GI165377202, Length=206, Percent_Identity=36.4077669902913, Blast_Score=101, Evalue=1e-21,
Organism=Escherichia coli, GI1786270, Length=321, Percent_Identity=35.202492211838, Blast_Score=151, Evalue=5e-38,
Organism=Drosophila melanogaster, GI62472493, Length=339, Percent_Identity=35.693215339233, Blast_Score=145, Evalue=4e-35,
Organism=Drosophila melanogaster, GI28571637, Length=339, Percent_Identity=35.693215339233, Blast_Score=145, Evalue=4e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_LEPBA (B0S989)

Other databases:

- EMBL:   CP000777
- RefSeq:   YP_001962794.1
- ProteinModelPortal:   B0S989
- SMR:   B0S989
- GeneID:   6388210
- GenomeReviews:   CP000777_GR
- KEGG:   lbf:LBF_1709
- HOGENOM:   HBG302779
- OMA:   PQLDDPE
- ProtClustDB:   CLSK574166
- BioCyc:   LBIF355278:LBF_1709-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 35550; Mature: 35550

Theoretical pI: Translated: 8.82; Mature: 8.82

Prosite motif: NA

Important sites: BINDING 56-56 BINDING 86-86 BINDING 108-108 BINDING 115-115

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLESPHIPVLPSEVISLLQQTKNPNPQWFLDGTAGEGGHSKLILKTFPEAKLILIDRDAV
CCCCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCCEEEEEECCCCEEEEEECHHH
MLERAKKEILKEIGSLDRVHAFQMNFSEVDSELLQEVGCPSLDGALVDLGVSLFHFLHSG
HHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCC
RGFTFKNDEPLDMRLEANIGGKTAADVVNYSSVLHLKKVFWEYGEERWALKIANNIVQTR
CCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
HKKKFGTNTDLVKLVEASIPRKFWPKESHPATRIFQALRIEVNEELVHAEKGIRALAQCL
HHHHCCCCHHHHHHHHHHCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GIGGVLTCISFHSLEDRIVKWTFRDLKDNGPFEILTKKPILPSETEIKENRASRSAKLRG
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHC
LMKINPIKESRWEK
CEEECCCCCCCCCC
>Mature Secondary Structure
MLESPHIPVLPSEVISLLQQTKNPNPQWFLDGTAGEGGHSKLILKTFPEAKLILIDRDAV
CCCCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCCEEEEEECCCCEEEEEECHHH
MLERAKKEILKEIGSLDRVHAFQMNFSEVDSELLQEVGCPSLDGALVDLGVSLFHFLHSG
HHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCC
RGFTFKNDEPLDMRLEANIGGKTAADVVNYSSVLHLKKVFWEYGEERWALKIANNIVQTR
CCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
HKKKFGTNTDLVKLVEASIPRKFWPKESHPATRIFQALRIEVNEELVHAEKGIRALAQCL
HHHHCCCCHHHHHHHHHHCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GIGGVLTCISFHSLEDRIVKWTFRDLKDNGPFEILTKKPILPSETEIKENRASRSAKLRG
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHC
LMKINPIKESRWEK
CEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA