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The map label for this gene is murE [H]

Identifier: 183221146

GI number: 183221146

Start: 1838643

End: 1840169

Strand: Reverse

Name: murE [H]

Synonym: LEPBI_I1760

Alternate gene names: 183221146

Gene position: 1840169-1838643 (Counterclockwise)

Preceding gene: 183221147

Following gene: 183221145

Centisome position: 51.12

GC content: 38.24

Gene sequence:

>1527_bases
ATGAAGCTAACTGAAATTATCAAAAAAATTCCTGAGCTCAAACTCATCAAAGGAGATGGAGATTTAGACGTAGGATACAT
CTGGGCAGATAGCCGAAAGCTGAAACCAAATGATGTTTTTGTACTACCGGAAGACAAAGCTGAGACAATCGAGTCTTATT
TGCAAATGGCTTACGATAAAGGTGTCAAAGTGGTTCTTGTATCCAAACGGAACCTCAAATTAAAAAATCTAGATTTATTT
CCGACCATACTCGAATCAGAAGATGCGTTAGGTGATGTTCATGGAAAAATGGCATCACAACTCTTAGGGAACCCATCTAA
AAAATTAAAAATCATTGGGATCACTGGCACAAATGGAAAAACATCTTTAACTTTTATTTTATTCCACATTGCCAAAAAAT
TAAAGAAAAAAGTTGCACTCATAGGTACTGTCCAAATCCAAATTTTAGACCGAATTTTAGAATCAGGTTATACCACACCA
GATGCATCTTCTCTCAATTTATTACTCAGAGAGATGTTAGACGAAGGTGTAGAATATGTTTTTATGGAGATAAGTAGCCA
TGGATTAAAATTGGGAAGAGTGGCAGGAATCGAAATCACCTGTGCTGGTTTCACCAATTTAACCCAAGACCATTTAGACT
TTCATGAAAGTATGGAAGACTATTTTGAAAGTAAGTTTAAAATTTTCCAACTCCTCGAACAATCGTCAGTCAAAAACAAA
TTTGGTTTGGTTGCAGGTGATGTTTCATTTGGTGATCGAATGATCCAAAGAATACGCGAGTCGAAATTAAAAACTCCCAT
TTATATTCTTGGCAAATCGGGTGAATTCCATTATAGCAATACAAAACTCTCTCTTCTTGGAAGCGAATACCGTTTCCATA
AAAAAGAAAAAAATCTTCCTTTTATTGAAGTCAGAAGCATTCGCACCAATTTACTCGGAAACTTCAACGTGTTTAATACT
GCCTTTGCCATGTCCATTGCTTATGAACTTGGATTTCCTTGGGATGAGGTTGTTACGGCGATCGAAGAAATTCCCACTGT
ACCTGGACGTTTTCAAGTCATCCCATACCCTGACAAAACTCGGATCGCCGTTGTGGATTATGCACACACTCCTGATGCGT
TGGAAAATATTCTGAAGAGTTGTGTTGAAATTCGTCCCAAACAACTCATCTGTTTGTTTGGTTGTGGCGGTGATCGGGAT
CGTACCAAACGACCACAAATGGCAAAAATTGCAGAAACCTTGGCTGATTTTGTTTTCCTGACTTCCGACAATCCAAGAAC
TGAGGATCCTGAGTCCATCTTGGATGAAATCGAATCAGGTTTTTCTCGTGGTTTCAAACGGTATGAGAAGATCACTGACC
GAAGGGTTGCCATTCAGCGAGCAGTTTCCCTTTTGGAAAAAGACGGCATTTTGGTCGTTGCAGGAAAGGGACACGAAACT
TACCAAATCATCGGCAAAGAAAAAACCAATTTTGTCGACTTTTTAGAAATTGAAAATGCGTTTTTAAATTTAGATACCGG
GTTATAG

Upstream 100 bases:

>100_bases
TGTATCGAAGGACTTATCAATTTTTACCCATTAGTTTAGGGAATAGGACAGTAACGATTGAACTTCCCCCCATACCTGAG
ATGCAAGCAAAATCAAAACC

Downstream 100 bases:

>100_bases
AGGATTTAGTATGTTCCAATGGATTTATGAATCATTTGGTAATGATTATGGTTTTTTAAGAGTGTTTAGTTATGTAACCC
TTCGGGCAATGATGGCGGGA

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 508; Mature: 508

Protein sequence:

>508_residues
MKLTEIIKKIPELKLIKGDGDLDVGYIWADSRKLKPNDVFVLPEDKAETIESYLQMAYDKGVKVVLVSKRNLKLKNLDLF
PTILESEDALGDVHGKMASQLLGNPSKKLKIIGITGTNGKTSLTFILFHIAKKLKKKVALIGTVQIQILDRILESGYTTP
DASSLNLLLREMLDEGVEYVFMEISSHGLKLGRVAGIEITCAGFTNLTQDHLDFHESMEDYFESKFKIFQLLEQSSVKNK
FGLVAGDVSFGDRMIQRIRESKLKTPIYILGKSGEFHYSNTKLSLLGSEYRFHKKEKNLPFIEVRSIRTNLLGNFNVFNT
AFAMSIAYELGFPWDEVVTAIEEIPTVPGRFQVIPYPDKTRIAVVDYAHTPDALENILKSCVEIRPKQLICLFGCGGDRD
RTKRPQMAKIAETLADFVFLTSDNPRTEDPESILDEIESGFSRGFKRYEKITDRRVAIQRAVSLLEKDGILVVAGKGHET
YQIIGKEKTNFVDFLEIENAFLNLDTGL

Sequences:

>Translated_508_residues
MKLTEIIKKIPELKLIKGDGDLDVGYIWADSRKLKPNDVFVLPEDKAETIESYLQMAYDKGVKVVLVSKRNLKLKNLDLF
PTILESEDALGDVHGKMASQLLGNPSKKLKIIGITGTNGKTSLTFILFHIAKKLKKKVALIGTVQIQILDRILESGYTTP
DASSLNLLLREMLDEGVEYVFMEISSHGLKLGRVAGIEITCAGFTNLTQDHLDFHESMEDYFESKFKIFQLLEQSSVKNK
FGLVAGDVSFGDRMIQRIRESKLKTPIYILGKSGEFHYSNTKLSLLGSEYRFHKKEKNLPFIEVRSIRTNLLGNFNVFNT
AFAMSIAYELGFPWDEVVTAIEEIPTVPGRFQVIPYPDKTRIAVVDYAHTPDALENILKSCVEIRPKQLICLFGCGGDRD
RTKRPQMAKIAETLADFVFLTSDNPRTEDPESILDEIESGFSRGFKRYEKITDRRVAIQRAVSLLEKDGILVVAGKGHET
YQIIGKEKTNFVDFLEIENAFLNLDTGL
>Mature_508_residues
MKLTEIIKKIPELKLIKGDGDLDVGYIWADSRKLKPNDVFVLPEDKAETIESYLQMAYDKGVKVVLVSKRNLKLKNLDLF
PTILESEDALGDVHGKMASQLLGNPSKKLKIIGITGTNGKTSLTFILFHIAKKLKKKVALIGTVQIQILDRILESGYTTP
DASSLNLLLREMLDEGVEYVFMEISSHGLKLGRVAGIEITCAGFTNLTQDHLDFHESMEDYFESKFKIFQLLEQSSVKNK
FGLVAGDVSFGDRMIQRIRESKLKTPIYILGKSGEFHYSNTKLSLLGSEYRFHKKEKNLPFIEVRSIRTNLLGNFNVFNT
AFAMSIAYELGFPWDEVVTAIEEIPTVPGRFQVIPYPDKTRIAVVDYAHTPDALENILKSCVEIRPKQLICLFGCGGDRD
RTKRPQMAKIAETLADFVFLTSDNPRTEDPESILDEIESGFSRGFKRYEKITDRRVAIQRAVSLLEKDGILVVAGKGHET
YQIIGKEKTNFVDFLEIENAFLNLDTGL

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=478, Percent_Identity=31.7991631799163, Blast_Score=215, Evalue=4e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR005761 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 57290; Mature: 57290

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLTEIIKKIPELKLIKGDGDLDVGYIWADSRKLKPNDVFVLPEDKAETIESYLQMAYDK
CCHHHHHHHCCCEEEEECCCCEEEEEEEECCCCCCCCCEEEECCCHHHHHHHHHHHHHHC
GVKVVLVSKRNLKLKNLDLFPTILESEDALGDVHGKMASQLLGNPSKKLKIIGITGTNGK
CCEEEEEECCCCEEECCCCCHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCC
TSLTFILFHIAKKLKKKVALIGTVQIQILDRILESGYTTPDASSLNLLLREMLDEGVEYV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCHHEE
FMEISSHGLKLGRVAGIEITCAGFTNLTQDHLDFHESMEDYFESKFKIFQLLEQSSVKNK
EEEHHCCCCEEEEEECEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
FGLVAGDVSFGDRMIQRIRESKLKTPIYILGKSGEFHYSNTKLSLLGSEYRFHKKEKNLP
CCEEECCCHHHHHHHHHHHHHHCCCCEEEEECCCCEEECCCEEEEECCHHHHHHHCCCCC
FIEVRSIRTNLLGNFNVFNTAFAMSIAYELGFPWDEVVTAIEEIPTVPGRFQVIPYPDKT
EEEHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCEEEEECCCCC
RIAVVDYAHTPDALENILKSCVEIRPKQLICLFGCGGDRDRTKRPQMAKIAETLADFVFL
EEEEEEECCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHEEEE
TSDNPRTEDPESILDEIESGFSRGFKRYEKITDRRVAIQRAVSLLEKDGILVVAGKGHET
ECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCE
YQIIGKEKTNFVDFLEIENAFLNLDTGL
EEEECCCCCCCCHHEECCCCEEECCCCC
>Mature Secondary Structure
MKLTEIIKKIPELKLIKGDGDLDVGYIWADSRKLKPNDVFVLPEDKAETIESYLQMAYDK
CCHHHHHHHCCCEEEEECCCCEEEEEEEECCCCCCCCCEEEECCCHHHHHHHHHHHHHHC
GVKVVLVSKRNLKLKNLDLFPTILESEDALGDVHGKMASQLLGNPSKKLKIIGITGTNGK
CCEEEEEECCCCEEECCCCCHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCC
TSLTFILFHIAKKLKKKVALIGTVQIQILDRILESGYTTPDASSLNLLLREMLDEGVEYV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCHHEE
FMEISSHGLKLGRVAGIEITCAGFTNLTQDHLDFHESMEDYFESKFKIFQLLEQSSVKNK
EEEHHCCCCEEEEEECEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
FGLVAGDVSFGDRMIQRIRESKLKTPIYILGKSGEFHYSNTKLSLLGSEYRFHKKEKNLP
CCEEECCCHHHHHHHHHHHHHHCCCCEEEEECCCCEEECCCEEEEECCHHHHHHHCCCCC
FIEVRSIRTNLLGNFNVFNTAFAMSIAYELGFPWDEVVTAIEEIPTVPGRFQVIPYPDKT
EEEHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCEEEEECCCCC
RIAVVDYAHTPDALENILKSCVEIRPKQLICLFGCGGDRDRTKRPQMAKIAETLADFVFL
EEEEEEECCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHEEEE
TSDNPRTEDPESILDEIESGFSRGFKRYEKITDRRVAIQRAVSLLEKDGILVVAGKGHET
ECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCE
YQIIGKEKTNFVDFLEIENAFLNLDTGL
EEEECCCCCCCCHHEECCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA