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The map label for this gene is yifB [H]

Identifier: 183221058

GI number: 183221058

Start: 1742171

End: 1743616

Strand: Reverse

Name: yifB [H]

Synonym: LEPBI_I1671

Alternate gene names: 183221058

Gene position: 1743616-1742171 (Counterclockwise)

Preceding gene: 183221073

Following gene: 183221029

Centisome position: 48.44

GC content: 42.12

Gene sequence:

>1446_bases
GTGAGCCCCCCCAAACGAGCAGAAGTTGGAAGTTTGATGTATGAATGGAATGGGACAAAAGAAATCCAAGTGGAGGTGGG
AATCCGAAGGGGATTTCCCCATTTCCAAATTTTAGGCAATGTCCCACAAGAAGCAAAGGAAGCTCGAGACCGCATCCGAT
TGGCTTTAGATGCCTCTCATTTCGATTTTCCGATGGAAACCATCATCATCAATGTCAAACCCTCCCATATCCAAAAGAAG
AAGGTATCCTTGGACCTGGCAATGGCTGTGGGAATCTTACAGGCGACGGATCAAATCAAACGACCCAATCGAAGGATTGT
GTATTTGGGAAATCTCGGCCTCGATGGAAGCCTTGTGGGGGGAAAGGAGCTCCTTCCCTTCCTCTGGCAAAAGGCGGAAA
AGAAAGACCAAGTCTATTGCCTTCCCGATTCGTTACGAAACGAATCCTTACCTGATGGAGAATATTATTTTCTCACCGAC
TTACAAGAATTAGAGAGTCTGAGTCAAAAACCACCGGATGTTCAGAAAAAAACATACACAATGGAAAACCCAGTTTGGGA
AGAAGTGTATCTCGATCCTTTTCAGATGAATGCCTTCCAAGGCCTTCTTTATGCCTTGCTTGGGAACCACCACAGTCTCT
TACTGGGAAGTCCTGGTGTTGGAAAGACCATGTTACACCGATTACTCGAACCACTCCTTCCTCCGAAACTAACTGGGGAA
CAATCAAATCTGGGGATTTGGCGGGCAAACGGAGAGTTTGAAGTTCCTACAAATAAAAGACCTTTTCGTAGTCCCCATCA
TTCCACAACAGAGGTAGGACTTTTAGGTGGAGGTCTCCCCTACCAACCTGGAGAAATCACACGAGCAGAGGGAGGGATTT
TGTTTCTGGATGAAGCTCTTGAATTCAAGGATCGAATTTTAGAAAGTTTACGAATGCCGATGGAAGATTCTTATTTGGAA
ATCACTCGTCTGAATGAAGTCACAAAAATGAAAACCGATTTCACTTTGTTTTTATCTTCCAATCCGTGCCCATGTGGCAA
TTACCAAAGTCAGAACCACTGCCATTGTTCCTTACAAAAGATCCGATTGTATTTACAAAAAATCAGCGGAGCCTTTTTGG
ATCGGATCACTATCTTCCAAACTTTGTTTGTTTCGAACGTAGAGCGAAACATTCGTTTGGAGGAAGAAAAACTAAAACGG
ATTGTGAATGAAAGATTTGATTTTAAAAAAAATAGAATCAATTCGAATGAGGAAACTAAAAAAATATTACAACAATTAGA
TATAAGTAAAGAAACCAAACATTTATCCCTACGAAAGAAAAAACAAATTGTTTCTCTCGCAAGGACCATTGCTGATTGGA
ACCTCTCCCCTAGAACAAAGGAAGTACATATTCAAGAGGCTTTGGATTATTCCTTGGGATACCAGTGGATCTATAGCCTA
GGTTGA

Upstream 100 bases:

>100_bases
CCAAAGCAAACTCTCAGGAAAAGTCACCATGATGCCTTAATTATGAAAAGTCTTTTTTATTTTTTGAACCAAAAGCGATC
CAATCGAACCTACCTCAGAT

Downstream 100 bases:

>100_bases
TTATTTGGAAATAAAATGTGAAATTTCCATGGCGATTTTATTGATCGCATAATCCATATAGGCAGGGTCATCGATTTTTT
TCTTATAGGTATCGAATTTT

Product: putative ATPase with chaperone activity

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 481; Mature: 480

Protein sequence:

>481_residues
MSPPKRAEVGSLMYEWNGTKEIQVEVGIRRGFPHFQILGNVPQEAKEARDRIRLALDASHFDFPMETIIINVKPSHIQKK
KVSLDLAMAVGILQATDQIKRPNRRIVYLGNLGLDGSLVGGKELLPFLWQKAEKKDQVYCLPDSLRNESLPDGEYYFLTD
LQELESLSQKPPDVQKKTYTMENPVWEEVYLDPFQMNAFQGLLYALLGNHHSLLLGSPGVGKTMLHRLLEPLLPPKLTGE
QSNLGIWRANGEFEVPTNKRPFRSPHHSTTEVGLLGGGLPYQPGEITRAEGGILFLDEALEFKDRILESLRMPMEDSYLE
ITRLNEVTKMKTDFTLFLSSNPCPCGNYQSQNHCHCSLQKIRLYLQKISGAFLDRITIFQTLFVSNVERNIRLEEEKLKR
IVNERFDFKKNRINSNEETKKILQQLDISKETKHLSLRKKKQIVSLARTIADWNLSPRTKEVHIQEALDYSLGYQWIYSL
G

Sequences:

>Translated_481_residues
MSPPKRAEVGSLMYEWNGTKEIQVEVGIRRGFPHFQILGNVPQEAKEARDRIRLALDASHFDFPMETIIINVKPSHIQKK
KVSLDLAMAVGILQATDQIKRPNRRIVYLGNLGLDGSLVGGKELLPFLWQKAEKKDQVYCLPDSLRNESLPDGEYYFLTD
LQELESLSQKPPDVQKKTYTMENPVWEEVYLDPFQMNAFQGLLYALLGNHHSLLLGSPGVGKTMLHRLLEPLLPPKLTGE
QSNLGIWRANGEFEVPTNKRPFRSPHHSTTEVGLLGGGLPYQPGEITRAEGGILFLDEALEFKDRILESLRMPMEDSYLE
ITRLNEVTKMKTDFTLFLSSNPCPCGNYQSQNHCHCSLQKIRLYLQKISGAFLDRITIFQTLFVSNVERNIRLEEEKLKR
IVNERFDFKKNRINSNEETKKILQQLDISKETKHLSLRKKKQIVSLARTIADWNLSPRTKEVHIQEALDYSLGYQWIYSL
G
>Mature_480_residues
SPPKRAEVGSLMYEWNGTKEIQVEVGIRRGFPHFQILGNVPQEAKEARDRIRLALDASHFDFPMETIIINVKPSHIQKKK
VSLDLAMAVGILQATDQIKRPNRRIVYLGNLGLDGSLVGGKELLPFLWQKAEKKDQVYCLPDSLRNESLPDGEYYFLTDL
QELESLSQKPPDVQKKTYTMENPVWEEVYLDPFQMNAFQGLLYALLGNHHSLLLGSPGVGKTMLHRLLEPLLPPKLTGEQ
SNLGIWRANGEFEVPTNKRPFRSPHHSTTEVGLLGGGLPYQPGEITRAEGGILFLDEALEFKDRILESLRMPMEDSYLEI
TRLNEVTKMKTDFTLFLSSNPCPCGNYQSQNHCHCSLQKIRLYLQKISGAFLDRITIFQTLFVSNVERNIRLEEEKLKRI
VNERFDFKKNRINSNEETKKILQQLDISKETKHLSLRKKKQIVSLARTIADWNLSPRTKEVHIQEALDYSLGYQWIYSLG

Specific function: Unknown

COG id: COG0606

COG function: function code O; Predicted ATPase with chaperone activity

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Mg-chelatase subunits D/I family. ComM subfamily [H]

Homologues:

Organism=Escherichia coli, GI145693202, Length=478, Percent_Identity=30.1255230125523, Blast_Score=188, Evalue=6e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR004482
- InterPro:   IPR000523
- InterPro:   IPR020568
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01078 Mg_chelatase [H]

EC number: NA

Molecular weight: Translated: 55204; Mature: 55073

Theoretical pI: Translated: 8.42; Mature: 8.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPPKRAEVGSLMYEWNGTKEIQVEVGIRRGFPHFQILGNVPQEAKEARDRIRLALDASH
CCCCCCCCCCCEEEEECCCEEEEEEEECCCCCCCEEEECCCCHHHHHHHHHEEEEEECCC
FDFPMETIIINVKPSHIQKKKVSLDLAMAVGILQATDQIKRPNRRIVYLGNLGLDGSLVG
CCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCC
GKELLPFLWQKAEKKDQVYCLPDSLRNESLPDGEYYFLTDLQELESLSQKPPDVQKKTYT
HHHHHHHHHHHCCCCCCEEECCHHHCCCCCCCCCEEEECCHHHHHHHHCCCCCCCHHEEE
MENPVWEEVYLDPFQMNAFQGLLYALLGNHHSLLLGSPGVGKTMLHRLLEPLLPPKLTGE
CCCCCHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCCC
QSNLGIWRANGEFEVPTNKRPFRSPHHSTTEVGLLGGGLPYQPGEITRAEGGILFLDEAL
CCCCEEEECCCEEECCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCEECCCCEEEEHHHH
EFKDRILESLRMPMEDSYLEITRLNEVTKMKTDFTLFLSSNPCPCGNYQSQNHCHCSLQK
HHHHHHHHHHCCCCCCCEEEEHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCHHHHHHH
IRLYLQKISGAFLDRITIFQTLFVSNVERNIRLEEEKLKRIVNERFDFKKNRINSNEETK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECHHHHHHHHHHHHCCHHHCCCCCHHHH
KILQQLDISKETKHLSLRKKKQIVSLARTIADWNLSPRTKEVHIQEALDYSLGYQWIYSL
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHCCCEEEEC
G
C
>Mature Secondary Structure 
SPPKRAEVGSLMYEWNGTKEIQVEVGIRRGFPHFQILGNVPQEAKEARDRIRLALDASH
CCCCCCCCCCEEEEECCCEEEEEEEECCCCCCCEEEECCCCHHHHHHHHHEEEEEECCC
FDFPMETIIINVKPSHIQKKKVSLDLAMAVGILQATDQIKRPNRRIVYLGNLGLDGSLVG
CCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCC
GKELLPFLWQKAEKKDQVYCLPDSLRNESLPDGEYYFLTDLQELESLSQKPPDVQKKTYT
HHHHHHHHHHHCCCCCCEEECCHHHCCCCCCCCCEEEECCHHHHHHHHCCCCCCCHHEEE
MENPVWEEVYLDPFQMNAFQGLLYALLGNHHSLLLGSPGVGKTMLHRLLEPLLPPKLTGE
CCCCCHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCCC
QSNLGIWRANGEFEVPTNKRPFRSPHHSTTEVGLLGGGLPYQPGEITRAEGGILFLDEAL
CCCCEEEECCCEEECCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCEECCCCEEEEHHHH
EFKDRILESLRMPMEDSYLEITRLNEVTKMKTDFTLFLSSNPCPCGNYQSQNHCHCSLQK
HHHHHHHHHHCCCCCCCEEEEHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCHHHHHHH
IRLYLQKISGAFLDRITIFQTLFVSNVERNIRLEEEKLKRIVNERFDFKKNRINSNEETK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECHHHHHHHHHHHHCCHHHCCCCCHHHH
KILQQLDISKETKHLSLRKKKQIVSLARTIADWNLSPRTKEVHIQEALDYSLGYQWIYSL
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHCCCEEEEC
G
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609 [H]