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The map label for this gene is fmt [H]

Identifier: 183221044

GI number: 183221044

Start: 1733686

End: 1734654

Strand: Direct

Name: fmt [H]

Synonym: LEPBI_I1657

Alternate gene names: 183221044

Gene position: 1733686-1734654 (Clockwise)

Preceding gene: 183221043

Following gene: 183221045

Centisome position: 48.16

GC content: 46.03

Gene sequence:

>969_bases
ATGAAACTATCAATTGGATACTTTGGATCTCCCGAACATTCAAAAGAATTATTACAAATGATTTTGGATGCGGGAATCCA
AGTGGATTTTGTTGTCACCAATGTGGACAAACCGGTGGGGCGTAAACAAATCATCACGCCCACTCCCGTGAAACAACTTG
CAGAAGAAAAAGGAATCCCTGTGATCCAATCGGTCCGTCTTCGCACTGACGATGCCGCACAAAAACAAATTCTATCGTTC
CGATCCCCGGTCCATGTGGTGTATGCCTACGGATCCATAGTCCCTGAAACGGTATTTATGGACCCTAAATGGGGCAGTAT
CAACCTTCATGGGAGTTTACTTCCGAAGTATAGGGGTGCCTCTCCCGTCCAAACTGTTTTACTTACGGGAGAGAAGACCA
CTGGGTTTACCATCCAATACTTGGCAAAGGAAGTGGATTCGGGGGACATCATTTCCCAAAAATCATGGACTGTATCCTTA
GAAGAAACAACGGGTTCCCTCTTAAAAACCATCACCAAAGAGGGAGGTGTGGAACTCATACGCCTCTTACAAACATTGGA
ATCCACAGGGGAAGGTTGGAAGTCCACCCCACAAAAATCGGAAGAGGCCACCCATTGCCAAAAGATCACGGCAAATCATA
GGCCAATCGATTGGTCCAGGTCGGCAAAAGAGATCCACAACCAAATCCGGGCTCTAAACCCAGACCCACTGGCCACTACC
GAGTTTCGAGGGAAAAAACTGATTCTGGTTTCCTCGTTTTTACCCGAAACCCAAACAGAACCCATCCAAATCCCAATTGA
GGCGAAAACAGGTTCCTTTTTTCTTTACCAGAAAAAAAGGCTTTTCTGTCTCTGTGGAGACGGAAACCTGCTTGGTATAG
ATACCTTACAACCCGAAGGGAAAAAACCCATGAAGGGATTTGAATTTTTTAATGGGGCGCGGGTTTTGACCGGAGAATCG
TTTACGTGA

Upstream 100 bases:

>100_bases
ACGAAATCTTTTAAAAGAAACAAAACAAACGTTCAAACTGGATTCTAAATGTTATATCGAATATGATTTGGATCCTATGG
AACTTGTGTAAGGAAAGGAT

Downstream 100 bases:

>100_bases
AAGAAAAGTTTCTAAAAATTTTACCTTACAGTGGTTATGTTCTATTTGTTGGTCTCGGACTCCTCGTTTTTTTTGTCGCA
GCCTTCCTTGTGGTGGTTGT

Product: methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MKLSIGYFGSPEHSKELLQMILDAGIQVDFVVTNVDKPVGRKQIITPTPVKQLAEEKGIPVIQSVRLRTDDAAQKQILSF
RSPVHVVYAYGSIVPETVFMDPKWGSINLHGSLLPKYRGASPVQTVLLTGEKTTGFTIQYLAKEVDSGDIISQKSWTVSL
EETTGSLLKTITKEGGVELIRLLQTLESTGEGWKSTPQKSEEATHCQKITANHRPIDWSRSAKEIHNQIRALNPDPLATT
EFRGKKLILVSSFLPETQTEPIQIPIEAKTGSFFLYQKKRLFCLCGDGNLLGIDTLQPEGKKPMKGFEFFNGARVLTGES
FT

Sequences:

>Translated_322_residues
MKLSIGYFGSPEHSKELLQMILDAGIQVDFVVTNVDKPVGRKQIITPTPVKQLAEEKGIPVIQSVRLRTDDAAQKQILSF
RSPVHVVYAYGSIVPETVFMDPKWGSINLHGSLLPKYRGASPVQTVLLTGEKTTGFTIQYLAKEVDSGDIISQKSWTVSL
EETTGSLLKTITKEGGVELIRLLQTLESTGEGWKSTPQKSEEATHCQKITANHRPIDWSRSAKEIHNQIRALNPDPLATT
EFRGKKLILVSSFLPETQTEPIQIPIEAKTGSFFLYQKKRLFCLCGDGNLLGIDTLQPEGKKPMKGFEFFNGARVLTGES
FT
>Mature_322_residues
MKLSIGYFGSPEHSKELLQMILDAGIQVDFVVTNVDKPVGRKQIITPTPVKQLAEEKGIPVIQSVRLRTDDAAQKQILSF
RSPVHVVYAYGSIVPETVFMDPKWGSINLHGSLLPKYRGASPVQTVLLTGEKTTGFTIQYLAKEVDSGDIISQKSWTVSL
EETTGSLLKTITKEGGVELIRLLQTLESTGEGWKSTPQKSEEATHCQKITANHRPIDWSRSAKEIHNQIRALNPDPLATT
EFRGKKLILVSSFLPETQTEPIQIPIEAKTGSFFLYQKKRLFCLCGDGNLLGIDTLQPEGKKPMKGFEFFNGARVLTGES
FT

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family [H]

Homologues:

Organism=Homo sapiens, GI238814322, Length=326, Percent_Identity=27.9141104294479, Blast_Score=99, Evalue=6e-21,
Organism=Homo sapiens, GI21614513, Length=276, Percent_Identity=28.9855072463768, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI164663775, Length=326, Percent_Identity=28.8343558282209, Blast_Score=78, Evalue=9e-15,
Organism=Escherichia coli, GI1789683, Length=314, Percent_Identity=35.3503184713376, Blast_Score=182, Evalue=3e-47,
Organism=Escherichia coli, GI1788589, Length=275, Percent_Identity=28, Blast_Score=94, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI133930964, Length=310, Percent_Identity=23.8709677419355, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI28571984, Length=229, Percent_Identity=30.1310043668122, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI45550868, Length=229, Percent_Identity=30.1310043668122, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24585660, Length=243, Percent_Identity=24.2798353909465, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR015518 [H]

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N [H]

EC number: =2.1.2.9 [H]

Molecular weight: Translated: 35686; Mature: 35686

Theoretical pI: Translated: 8.76; Mature: 8.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLSIGYFGSPEHSKELLQMILDAGIQVDFVVTNVDKPVGRKQIITPTPVKQLAEEKGIP
CEEEECCCCCCHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCC
VIQSVRLRTDDAAQKQILSFRSPVHVVYAYGSIVPETVFMDPKWGSINLHGSLLPKYRGA
EEEHEEECCCHHHHHHHHHHCCCEEEEEEECCCCCCEEEECCCCCEEEECCCCCCCCCCC
SPVQTVLLTGEKTTGFTIQYLAKEVDSGDIISQKSWTVSLEETTGSLLKTITKEGGVELI
CCEEEEEEECCCCCCCHHHHHHHHCCCCCEEECCCEEEEEHHHHHHHHHHHHHCCCHHHH
RLLQTLESTGEGWKSTPQKSEEATHCQKITANHRPIDWSRSAKEIHNQIRALNPDPLATT
HHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCC
EFRGKKLILVSSFLPETQTEPIQIPIEAKTGSFFLYQKKRLFCLCGDGNLLGIDTLQPEG
CCCCCEEEEEECCCCCCCCCCEEEEEEECCCCEEEEECCEEEEEECCCCEEEEECCCCCC
KKPMKGFEFFNGARVLTGESFT
CCCCCCHHHCCCCEEECCCCCC
>Mature Secondary Structure
MKLSIGYFGSPEHSKELLQMILDAGIQVDFVVTNVDKPVGRKQIITPTPVKQLAEEKGIP
CEEEECCCCCCHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCC
VIQSVRLRTDDAAQKQILSFRSPVHVVYAYGSIVPETVFMDPKWGSINLHGSLLPKYRGA
EEEHEEECCCHHHHHHHHHHCCCEEEEEEECCCCCCEEEECCCCCEEEECCCCCCCCCCC
SPVQTVLLTGEKTTGFTIQYLAKEVDSGDIISQKSWTVSLEETTGSLLKTITKEGGVELI
CCEEEEEEECCCCCCCHHHHHHHHCCCCCEEECCCEEEEEHHHHHHHHHHHHHCCCHHHH
RLLQTLESTGEGWKSTPQKSEEATHCQKITANHRPIDWSRSAKEIHNQIRALNPDPLATT
HHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCC
EFRGKKLILVSSFLPETQTEPIQIPIEAKTGSFFLYQKKRLFCLCGDGNLLGIDTLQPEG
CCCCCEEEEEECCCCCCCCCCEEEEEEECCCCEEEEECCEEEEEECCCCEEEEECCCCCC
KKPMKGFEFFNGARVLTGESFT
CCCCCCHHHCCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA