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The map label for this gene is priA [H]

Identifier: 183221043

GI number: 183221043

Start: 1731724

End: 1733676

Strand: Direct

Name: priA [H]

Synonym: LEPBI_I1656

Alternate gene names: 183221043

Gene position: 1731724-1733676 (Clockwise)

Preceding gene: 183221042

Following gene: 183221044

Centisome position: 48.11

GC content: 40.96

Gene sequence:

>1953_bases
ATGATCCAGTATGCGGAAGTTGCCTTAAATCTTTCTTGGGAAAGTAAAACCTTAACCTACGAGATTCCAAATTCGATTAC
CGCTTTACAACCAGGTGTTCGGGTTCTTGTTCCCTTAAACGGAAAAGAATGGGATGGAGTGGTGATTCAGATCCACAATA
ACGAACCCAACTATGAAACACTTTCGGTCTTAAAACAAATTGATCCCGAACCTGTTATCACAACAGAACAATTGGAACTG
GCAACATGGATGTCTGAGAATTATCTTTCCTCTCTTGGGGAAGCATTGTTTCTTATGGTTCCCAAAGGGAAAAAAAGGAA
ACTCACGAAAGAAGTTGATACGGAAGTCCAATCCCACTTATTACACCCGTTAAATGAAGCACAAAAAAAAGCTTATACTG
AGATCAAACAGAATACACATGCGAATACTCATCTTTTGTATGGAATCACGGGAAGTGGGAAAACGGAAGTTTACCTTCAT
TTGATGCGAGACATTTTGCAAGAACCTAAGGGTTCGGTTATTTTCCTTGTCCCAGAAATTTCTTTAACCTATCCTACGAT
CGCTCGGATCGAGGCCATTTTCCCAAACCAAGTGGCAGTTTTACATTCGCATTTACGGATTTCAGAAAAGTTCCAAAACT
ATTTGGATTTAAAGGAAGGAAAAAAAAGGATCTGCATTGGAACTCGGTCGGCAATTTTTGCGCCCCTTTCTGACCTGAAA
CTAGTGGTAATGGATGAGGAACATGACGCCTCTTATAAAGAAAATGGTTCTCCTCGTTACCATGCAAGGCAGGTCGCCTT
ACAAAGGATTTTGAAAACGGGTGGCAAACTCCTACTGGGTTCGGCCACACCGAGTATCGAGTTGTATTACTTAGCTAAAT
CAGGACAAATTGGATTTTCCATTTTGGAACAACGTGCCAATCCCAAAGCCAAACTTCCTTTCGTAGAGATGTCTGATAAA
CAAGATGATAAACATTTGATCTCAGGGGACTTACAATTTAAAATTTCTGACCGATTGAAAAAAAAAGAACAAATCATCTT
ACTCCTGAACCGTAGAGGGTATAACCCTTTTATTTTTTCACCGCAAACAAAGGAGTTTGTCCATTGTCCCAAATGTACGG
CAACTCTTTGTTACCATTCTGACCAAACGGTACGTTGCCATTTATGTGGCTATCATTCCAATTTTTACAATCTAAAACAG
ACGTTTGGTGAAGATTTAGAGCTTTTTGGAGCTGGCACACAAAAATTAGAGGAGTATCTCTTATCTCATTTTCCGAGTGC
AAGGATCGAACGCCTCGACCAAGACAGTTCCAAAAACAAAGATGTCACCCGCCTTGTGTTAGAGAAATTAGGAGAAGGGG
AACTTGATATCCTCACCGGTACCCAAATGATATCAAAAGGCTTGGATTATGCAAATGTGACTCTTGTGGGAATCCTTAAC
GCTAATCATGGGTTAGGTGTCCCTGACTTTCGAAGTAGCGAACGCACCTATGCCCTTGTCTCCCAAGTGGCGGGCCGGGC
GGGACGCGGTGAAAAACCAGGAGAAGTCATCATCCAGTCGAATGACCCGGAACACCCTGTCCTTAAAATGGCCAAGGAAC
AAAACTACCCCGCTTTTTTTGAATGGGAACTCCAATTTCGAAAAGATCTGTTTTACCCTCCCTTTGCGAGACTTGCTCGC
CTTGTCTTTCGTTCTAAATATGAAGAGGTGGCCAACAAACAATCCGTTGTCTATGGAGAGCTCATCAAAGAAAAAAAAGA
GGATTCAATCGTGATGCTTGGACCAAGCCAATGTCCGTTTTATAAAATTGATAATAACTATCGGTACCATATACTACTCA
AAGCAAAATCGATCACCGCTCTACGAAATCTTTTAAAAGAAACAAAACAAACGTTCAAACTGGATTCTAAATGTTATATC
GAATATGATTTGGATCCTATGGAACTTGTGTAA

Upstream 100 bases:

>100_bases
GCAGATTCGGAAACAAACCTTTTTCAATATGGAAAAGAAAAACAGGACTTAGAAGAAAGTTTTATCTCTCATTTATCGAT
TCTAAAACAATATCTATAGT

Downstream 100 bases:

>100_bases
GGAAAGGATATGAAACTATCAATTGGATACTTTGGATCTCCCGAACATTCAAAAGAATTATTACAAATGATTTTGGATGC
GGGAATCCAAGTGGATTTTG

Product: primosomal protein N'

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 650; Mature: 650

Protein sequence:

>650_residues
MIQYAEVALNLSWESKTLTYEIPNSITALQPGVRVLVPLNGKEWDGVVIQIHNNEPNYETLSVLKQIDPEPVITTEQLEL
ATWMSENYLSSLGEALFLMVPKGKKRKLTKEVDTEVQSHLLHPLNEAQKKAYTEIKQNTHANTHLLYGITGSGKTEVYLH
LMRDILQEPKGSVIFLVPEISLTYPTIARIEAIFPNQVAVLHSHLRISEKFQNYLDLKEGKKRICIGTRSAIFAPLSDLK
LVVMDEEHDASYKENGSPRYHARQVALQRILKTGGKLLLGSATPSIELYYLAKSGQIGFSILEQRANPKAKLPFVEMSDK
QDDKHLISGDLQFKISDRLKKKEQIILLLNRRGYNPFIFSPQTKEFVHCPKCTATLCYHSDQTVRCHLCGYHSNFYNLKQ
TFGEDLELFGAGTQKLEEYLLSHFPSARIERLDQDSSKNKDVTRLVLEKLGEGELDILTGTQMISKGLDYANVTLVGILN
ANHGLGVPDFRSSERTYALVSQVAGRAGRGEKPGEVIIQSNDPEHPVLKMAKEQNYPAFFEWELQFRKDLFYPPFARLAR
LVFRSKYEEVANKQSVVYGELIKEKKEDSIVMLGPSQCPFYKIDNNYRYHILLKAKSITALRNLLKETKQTFKLDSKCYI
EYDLDPMELV

Sequences:

>Translated_650_residues
MIQYAEVALNLSWESKTLTYEIPNSITALQPGVRVLVPLNGKEWDGVVIQIHNNEPNYETLSVLKQIDPEPVITTEQLEL
ATWMSENYLSSLGEALFLMVPKGKKRKLTKEVDTEVQSHLLHPLNEAQKKAYTEIKQNTHANTHLLYGITGSGKTEVYLH
LMRDILQEPKGSVIFLVPEISLTYPTIARIEAIFPNQVAVLHSHLRISEKFQNYLDLKEGKKRICIGTRSAIFAPLSDLK
LVVMDEEHDASYKENGSPRYHARQVALQRILKTGGKLLLGSATPSIELYYLAKSGQIGFSILEQRANPKAKLPFVEMSDK
QDDKHLISGDLQFKISDRLKKKEQIILLLNRRGYNPFIFSPQTKEFVHCPKCTATLCYHSDQTVRCHLCGYHSNFYNLKQ
TFGEDLELFGAGTQKLEEYLLSHFPSARIERLDQDSSKNKDVTRLVLEKLGEGELDILTGTQMISKGLDYANVTLVGILN
ANHGLGVPDFRSSERTYALVSQVAGRAGRGEKPGEVIIQSNDPEHPVLKMAKEQNYPAFFEWELQFRKDLFYPPFARLAR
LVFRSKYEEVANKQSVVYGELIKEKKEDSIVMLGPSQCPFYKIDNNYRYHILLKAKSITALRNLLKETKQTFKLDSKCYI
EYDLDPMELV
>Mature_650_residues
MIQYAEVALNLSWESKTLTYEIPNSITALQPGVRVLVPLNGKEWDGVVIQIHNNEPNYETLSVLKQIDPEPVITTEQLEL
ATWMSENYLSSLGEALFLMVPKGKKRKLTKEVDTEVQSHLLHPLNEAQKKAYTEIKQNTHANTHLLYGITGSGKTEVYLH
LMRDILQEPKGSVIFLVPEISLTYPTIARIEAIFPNQVAVLHSHLRISEKFQNYLDLKEGKKRICIGTRSAIFAPLSDLK
LVVMDEEHDASYKENGSPRYHARQVALQRILKTGGKLLLGSATPSIELYYLAKSGQIGFSILEQRANPKAKLPFVEMSDK
QDDKHLISGDLQFKISDRLKKKEQIILLLNRRGYNPFIFSPQTKEFVHCPKCTATLCYHSDQTVRCHLCGYHSNFYNLKQ
TFGEDLELFGAGTQKLEEYLLSHFPSARIERLDQDSSKNKDVTRLVLEKLGEGELDILTGTQMISKGLDYANVTLVGILN
ANHGLGVPDFRSSERTYALVSQVAGRAGRGEKPGEVIIQSNDPEHPVLKMAKEQNYPAFFEWELQFRKDLFYPPFARLAR
LVFRSKYEEVANKQSVVYGELIKEKKEDSIVMLGPSQCPFYKIDNNYRYHILLKAKSITALRNLLKETKQTFKLDSKCYI
EYDLDPMELV

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=538, Percent_Identity=34.3866171003717, Blast_Score=283, Evalue=2e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 74075; Mature: 74075

Theoretical pI: Translated: 8.03; Mature: 8.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQYAEVALNLSWESKTLTYEIPNSITALQPGVRVLVPLNGKEWDGVVIQIHNNEPNYET
CCEEEEEEEEEEECCCEEEEECCCCCEECCCCCEEEEECCCCCCCCEEEEEECCCCCHHH
LSVLKQIDPEPVITTEQLELATWMSENYLSSLGEALFLMVPKGKKRKLTKEVDTEVQSHL
HHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHH
LHPLNEAQKKAYTEIKQNTHANTHLLYGITGSGKTEVYLHLMRDILQEPKGSVIFLVPEI
HCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEECC
SLTYPTIARIEAIFPNQVAVLHSHLRISEKFQNYLDLKEGKKRICIGTRSAIFAPLSDLK
EECCCCHHHEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHCCCCCCE
LVVMDEEHDASYKENGSPRYHARQVALQRILKTGGKLLLGSATPSIELYYLAKSGQIGFS
EEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCEEEEEEECCCCCHHH
ILEQRANPKAKLPFVEMSDKQDDKHLISGDLQFKISDRLKKKEQIILLLNRRGYNPFIFS
HHHHCCCCCCCCCEEECCCCCCCCEEECCCEEEEEHHHCCCCCEEEEEEECCCCCCEEEC
PQTKEFVHCPKCTATLCYHSDQTVRCHLCGYHSNFYNLKQTFGEDLELFGAGTQKLEEYL
CCCCHHEECCCCEEEEEECCCCEEEEEEEECCCCHHHHHHHHCCCCEECCCCHHHHHHHH
LSHFPSARIERLDQDSSKNKDVTRLVLEKLGEGELDILTGTQMISKGLDYANVTLVGILN
HHHCCCHHHHHHCCCCCCCCHHHHHHHHHCCCCCEEEEECHHHHHCCCCCCCEEEEEEEE
ANHGLGVPDFRSSERTYALVSQVAGRAGRGEKPGEVIIQSNDPEHPVLKMAKEQNYPAFF
CCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCHHHHHHHCCCCCEEE
EWELQFRKDLFYPPFARLARLVFRSKYEEVANKQSVVYGELIKEKKEDSIVMLGPSQCPF
EEEEEEHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCEEEECCCCCCC
YKIDNNYRYHILLKAKSITALRNLLKETKQTFKLDSKCYIEYDLDPMELV
EEECCCEEEEEEEEECHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHCC
>Mature Secondary Structure
MIQYAEVALNLSWESKTLTYEIPNSITALQPGVRVLVPLNGKEWDGVVIQIHNNEPNYET
CCEEEEEEEEEEECCCEEEEECCCCCEECCCCCEEEEECCCCCCCCEEEEEECCCCCHHH
LSVLKQIDPEPVITTEQLELATWMSENYLSSLGEALFLMVPKGKKRKLTKEVDTEVQSHL
HHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHH
LHPLNEAQKKAYTEIKQNTHANTHLLYGITGSGKTEVYLHLMRDILQEPKGSVIFLVPEI
HCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEECC
SLTYPTIARIEAIFPNQVAVLHSHLRISEKFQNYLDLKEGKKRICIGTRSAIFAPLSDLK
EECCCCHHHEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHCCCCCCE
LVVMDEEHDASYKENGSPRYHARQVALQRILKTGGKLLLGSATPSIELYYLAKSGQIGFS
EEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCEEEEEEECCCCCHHH
ILEQRANPKAKLPFVEMSDKQDDKHLISGDLQFKISDRLKKKEQIILLLNRRGYNPFIFS
HHHHCCCCCCCCCEEECCCCCCCCEEECCCEEEEEHHHCCCCCEEEEEEECCCCCCEEEC
PQTKEFVHCPKCTATLCYHSDQTVRCHLCGYHSNFYNLKQTFGEDLELFGAGTQKLEEYL
CCCCHHEECCCCEEEEEECCCCEEEEEEEECCCCHHHHHHHHCCCCEECCCCHHHHHHHH
LSHFPSARIERLDQDSSKNKDVTRLVLEKLGEGELDILTGTQMISKGLDYANVTLVGILN
HHHCCCHHHHHHCCCCCCCCHHHHHHHHHCCCCCEEEEECHHHHHCCCCCCCEEEEEEEE
ANHGLGVPDFRSSERTYALVSQVAGRAGRGEKPGEVIIQSNDPEHPVLKMAKEQNYPAFF
CCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCHHHHHHHCCCCCEEE
EWELQFRKDLFYPPFARLARLVFRSKYEEVANKQSVVYGELIKEKKEDSIVMLGPSQCPF
EEEEEEHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCEEEECCCCCCC
YKIDNNYRYHILLKAKSITALRNLLKETKQTFKLDSKCYIEYDLDPMELV
EEECCCEEEEEEEEECHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9534248; 9384377; 9086272 [H]