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The map label for this gene is queF [H]

Identifier: 183221001

GI number: 183221001

Start: 1687763

End: 1688164

Strand: Reverse

Name: queF [H]

Synonym: LEPBI_I1614

Alternate gene names: 183221001

Gene position: 1688164-1687763 (Counterclockwise)

Preceding gene: 183221003

Following gene: 183221000

Centisome position: 46.9

GC content: 41.04

Gene sequence:

>402_bases
ATGTCGGAAAAAAAATCAGAATCTTCTTATGAGGACAAACAAGACCATATCCCGGCTTGGACCACCCCTGCGATCGAATG
GTTTGCCAATGTTTATGCCGGTAAAGAATACAATATCGAATTTACCATCCCTGAATTCACTGCCGTTTGCCCCAAAACGG
GTCTACCTGACTTTGGAACCATCTACATTGAATACATTCCCAGGGAAAAATGTGCTGAATTAAAATCTCTGAAAGAATAC
ATGATGGCCTACCGAAATGTAGGCATTTTCCACGAAAATGTAGTCAATAAAATCTTGGAAGACTTTGTCAAAGCCATCGA
TCCTCTCTATGCCAAGGTCATTGGTGATTATAATGTTCGCGGTGGAGTGAAAACGGTAGTGAAACGAGAGTACAAAGCAT
AA

Upstream 100 bases:

>100_bases
TGAGATGAGCATACTGGCCGCCGAAATCGACGACTGCGATTTTTTTATCACTTGTCATGGGATGGTTCCAAAATAATTTT
GTTTATGTCGGGGTAAACAA

Downstream 100 bases:

>100_bases
ATGGAAAACCTAATTGGGTATGTGGCAGCATTTTTAACAACGGTTTCCTTTTTGCCCCAAGTCTTACGAGTCGTCATGAC
AAAACAAACAAGGGACATCA

Product: 7-cyano-7-deazaguanine reductase

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine reductase; NADPH-dependent nitrile oxidoreductase; PreQ(0) reductase [H]

Number of amino acids: Translated: 133; Mature: 132

Protein sequence:

>133_residues
MSEKKSESSYEDKQDHIPAWTTPAIEWFANVYAGKEYNIEFTIPEFTAVCPKTGLPDFGTIYIEYIPREKCAELKSLKEY
MMAYRNVGIFHENVVNKILEDFVKAIDPLYAKVIGDYNVRGGVKTVVKREYKA

Sequences:

>Translated_133_residues
MSEKKSESSYEDKQDHIPAWTTPAIEWFANVYAGKEYNIEFTIPEFTAVCPKTGLPDFGTIYIEYIPREKCAELKSLKEY
MMAYRNVGIFHENVVNKILEDFVKAIDPLYAKVIGDYNVRGGVKTVVKREYKA
>Mature_132_residues
SEKKSESSYEDKQDHIPAWTTPAIEWFANVYAGKEYNIEFTIPEFTAVCPKTGLPDFGTIYIEYIPREKCAELKSLKEYM
MAYRNVGIFHENVVNKILEDFVKAIDPLYAKVIGDYNVRGGVKTVVKREYKA

Specific function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) [H]

COG id: COG0780

COG function: function code R; Enzyme related to GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family. QueF type 1 subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016856
- InterPro:   IPR020602 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =1.7.1.13 [H]

Molecular weight: Translated: 15344; Mature: 15212

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEKKSESSYEDKQDHIPAWTTPAIEWFANVYAGKEYNIEFTIPEFTAVCPKTGLPDFGT
CCCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHCCCCCCEEEECCHHHHHCCCCCCCCCCE
IYIEYIPREKCAELKSLKEYMMAYRNVGIFHENVVNKILEDFVKAIDPLYAKVIGDYNVR
EEEEECCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GGVKTVVKREYKA
CHHHHHHHHHCCC
>Mature Secondary Structure 
SEKKSESSYEDKQDHIPAWTTPAIEWFANVYAGKEYNIEFTIPEFTAVCPKTGLPDFGT
CCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHCCCCCCEEEECCHHHHHCCCCCCCCCCE
IYIEYIPREKCAELKSLKEYMMAYRNVGIFHENVVNKILEDFVKAIDPLYAKVIGDYNVR
EEEEECCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GGVKTVVKREYKA
CHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA