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The map label for this gene is tyrS [H]

Identifier: 183220957

GI number: 183220957

Start: 1638637

End: 1639863

Strand: Direct

Name: tyrS [H]

Synonym: LEPBI_I1570

Alternate gene names: 183220957

Gene position: 1638637-1639863 (Clockwise)

Preceding gene: 183220955

Following gene: 183220958

Centisome position: 45.52

GC content: 43.68

Gene sequence:

>1227_bases
ATGAAAACTGAAATAGAATTGAACCAAGAATTAGAAACCATCCGCCGTGGCACAGTCGAGATCATCAGTGAAGCGGAACT
GTTAGAAAAAATCAAATCCAAACCTTCCCTTACCATCAAGGCTGGTTTTGATCCCACAGCACCTGATTTGCACTTGGGCC
ATTTTGTCCTGCTTCGAAAATTAAAACACTTCCAAGACCTGGGGCATGAAGTCTGTTTTATGCTTGGTGATTTTACTGCC
ATGATCGGGGATCCAACGGGAAAGTCCGAAACAAGAAAACGTTTGTCCAAAGAAGAAGTTTTGGAAAATTCCAAAACCTA
CCAATCCCAAGTATTTAAAATCTTAGATCCTCAAAAAATCAAAATCCTCTATAACTCTCATTGGTGTTCCGAACTCAAAT
TTGAAGACGTCCTTGTCCTTACCTCCAAGTACACGGTTTCAAGGATGCTCGAAAGAGATGACTTTACGAAACGTCACAAA
GCGGGAACTCCCATTTCGATGATTGAATTTTTATACCCCCTTGTACAAGGGTATGATTCCGTTGCCATGAAATCGGATGT
CGAACTCGGAGGAACGGATCAAAAATTCAATATGTTAGTTGGCCGCGACTTACAGAGAGAATACGGTCAAAAGCCACAGG
CTGTCATCACCTTACCACTGCTCGTGGGTCTTGATGGTGTGAAAAAAATGTCCAAGTCACTTGGCAACTATGTAGGGATC
ATTGAAAAACCCATCGATATGTATGGGAAAATCATGTCGATCTCGGATGATTTGATGTGGAATTATTTTGAACTTCTCAC
AGACCTCCCTTTGGCGGAAGTAGAAACAAAAAAAGAGGGAATTCGCACGAAGGCCCTCCATCCAAAAGAGGTGAAAACGG
AATTGGCACTTCTCATCATGGACCAACTCCATCCCAAGGAAGACAATCGAAAAGCAGTGGAAGAGTGGACGGCCATCCAC
AATACCAAAAACCGAGCCTTACCGGATGAAATTCCAACTGAAACCCTAGACCCGTCGTATTTTGCAGAAAAACCGCCACT
CCTTGTCTATGTTTTGTCCCAACTCAAGTTCATCCCGAGTGTTTCGGAGGGGAGACGGCTCATCCAGGCAGGGGGATTGT
ATTTGGACGAGGAGAAAATTACGGACTCGAGCCTCGCTCTGGAACCAGGGAAAGAATACCTCATCCGCCAAGGGAAGAAA
GGTAAATTTTTAAAGATCAAATCCTAA

Upstream 100 bases:

>100_bases
CATAAGGGTTTTTACGTACTTTGGTGGTTGCCTATAGAGATTCTAAGGTTAGTGTTGTCAAAAGTCACCTTTTCTACCAT
CGAAAATTTTTGAAATTGTT

Downstream 100 bases:

>100_bases
AGGATGAGAGAGACGAGTGTAGAACCTTAAGTCCCTTTCCGATAATCGATACTAAGGAAACCCATACCTAAATGTCACTC
GATCCGACAAACGAAGAGAA

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS [H]

Number of amino acids: Translated: 408; Mature: 408

Protein sequence:

>408_residues
MKTEIELNQELETIRRGTVEIISEAELLEKIKSKPSLTIKAGFDPTAPDLHLGHFVLLRKLKHFQDLGHEVCFMLGDFTA
MIGDPTGKSETRKRLSKEEVLENSKTYQSQVFKILDPQKIKILYNSHWCSELKFEDVLVLTSKYTVSRMLERDDFTKRHK
AGTPISMIEFLYPLVQGYDSVAMKSDVELGGTDQKFNMLVGRDLQREYGQKPQAVITLPLLVGLDGVKKMSKSLGNYVGI
IEKPIDMYGKIMSISDDLMWNYFELLTDLPLAEVETKKEGIRTKALHPKEVKTELALLIMDQLHPKEDNRKAVEEWTAIH
NTKNRALPDEIPTETLDPSYFAEKPPLLVYVLSQLKFIPSVSEGRRLIQAGGLYLDEEKITDSSLALEPGKEYLIRQGKK
GKFLKIKS

Sequences:

>Translated_408_residues
MKTEIELNQELETIRRGTVEIISEAELLEKIKSKPSLTIKAGFDPTAPDLHLGHFVLLRKLKHFQDLGHEVCFMLGDFTA
MIGDPTGKSETRKRLSKEEVLENSKTYQSQVFKILDPQKIKILYNSHWCSELKFEDVLVLTSKYTVSRMLERDDFTKRHK
AGTPISMIEFLYPLVQGYDSVAMKSDVELGGTDQKFNMLVGRDLQREYGQKPQAVITLPLLVGLDGVKKMSKSLGNYVGI
IEKPIDMYGKIMSISDDLMWNYFELLTDLPLAEVETKKEGIRTKALHPKEVKTELALLIMDQLHPKEDNRKAVEEWTAIH
NTKNRALPDEIPTETLDPSYFAEKPPLLVYVLSQLKFIPSVSEGRRLIQAGGLYLDEEKITDSSLALEPGKEYLIRQGKK
GKFLKIKS
>Mature_408_residues
MKTEIELNQELETIRRGTVEIISEAELLEKIKSKPSLTIKAGFDPTAPDLHLGHFVLLRKLKHFQDLGHEVCFMLGDFTA
MIGDPTGKSETRKRLSKEEVLENSKTYQSQVFKILDPQKIKILYNSHWCSELKFEDVLVLTSKYTVSRMLERDDFTKRHK
AGTPISMIEFLYPLVQGYDSVAMKSDVELGGTDQKFNMLVGRDLQREYGQKPQAVITLPLLVGLDGVKKMSKSLGNYVGI
IEKPIDMYGKIMSISDDLMWNYFELLTDLPLAEVETKKEGIRTKALHPKEVKTELALLIMDQLHPKEDNRKAVEEWTAIH
NTKNRALPDEIPTETLDPSYFAEKPPLLVYVLSQLKFIPSVSEGRRLIQAGGLYLDEEKITDSSLALEPGKEYLIRQGKK
GKFLKIKS

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=397, Percent_Identity=26.7002518891688, Blast_Score=113, Evalue=4e-25,
Organism=Escherichia coli, GI1787925, Length=416, Percent_Identity=27.6442307692308, Blast_Score=126, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI25145802, Length=314, Percent_Identity=27.0700636942675, Blast_Score=96, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6325160, Length=444, Percent_Identity=25.9009009009009, Blast_Score=95, Evalue=2e-20,
Organism=Drosophila melanogaster, GI20130383, Length=424, Percent_Identity=25.2358490566038, Blast_Score=122, Evalue=4e-28,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 46488; Mature: 46488

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: PS50889 S4 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTEIELNQELETIRRGTVEIISEAELLEKIKSKPSLTIKAGFDPTAPDLHLGHFVLLRK
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHH
LKHFQDLGHEVCFMLGDFTAMIGDPTGKSETRKRLSKEEVLENSKTYQSQVFKILDPQKI
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCEE
KILYNSHWCSELKFEDVLVLTSKYTVSRMLERDDFTKRHKAGTPISMIEFLYPLVQGYDS
EEEECCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHH
VAMKSDVELGGTDQKFNMLVGRDLQREYGQKPQAVITLPLLVGLDGVKKMSKSLGNYVGI
HHHHCCCCCCCCCHHHHHHHCCHHHHHHCCCCCEEEEHHHHHCCHHHHHHHHHHHHHHHH
IEKPIDMYGKIMSISDDLMWNYFELLTDLPLAEVETKKEGIRTKALHPKEVKTELALLIM
HHCCHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHCCHHCCCCHHHHHHHHHHHHH
DQLHPKEDNRKAVEEWTAIHNTKNRALPDEIPTETLDPSYFAEKPPLLVYVLSQLKFIPS
HHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHCCCCCHHHHHHHHHHHCCC
VSEGRRLIQAGGLYLDEEKITDSSLALEPGKEYLIRQGKKGKFLKIKS
HHHHHHHHHHCCEEEEHHHCCCCCEECCCCHHHHHHCCCCCCEEEECC
>Mature Secondary Structure
MKTEIELNQELETIRRGTVEIISEAELLEKIKSKPSLTIKAGFDPTAPDLHLGHFVLLRK
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHH
LKHFQDLGHEVCFMLGDFTAMIGDPTGKSETRKRLSKEEVLENSKTYQSQVFKILDPQKI
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCEE
KILYNSHWCSELKFEDVLVLTSKYTVSRMLERDDFTKRHKAGTPISMIEFLYPLVQGYDS
EEEECCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHH
VAMKSDVELGGTDQKFNMLVGRDLQREYGQKPQAVITLPLLVGLDGVKKMSKSLGNYVGI
HHHHCCCCCCCCCHHHHHHHCCHHHHHHCCCCCEEEEHHHHHCCHHHHHHHHHHHHHHHH
IEKPIDMYGKIMSISDDLMWNYFELLTDLPLAEVETKKEGIRTKALHPKEVKTELALLIM
HHCCHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHCCHHCCCCHHHHHHHHHHHHH
DQLHPKEDNRKAVEEWTAIHNTKNRALPDEIPTETLDPSYFAEKPPLLVYVLSQLKFIPS
HHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHCCCCCHHHHHHHHHHHCCC
VSEGRRLIQAGGLYLDEEKITDSSLALEPGKEYLIRQGKKGKFLKIKS
HHHHHHHHHHCCEEEEHHHCCCCCEECCCCHHHHHHCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA