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The map label for this gene is rpoD [H]

Identifier: 183220954

GI number: 183220954

Start: 1633878

End: 1635650

Strand: Direct

Name: rpoD [H]

Synonym: LEPBI_I1567

Alternate gene names: 183220954

Gene position: 1633878-1635650 (Clockwise)

Preceding gene: 183220953

Following gene: 183220955

Centisome position: 45.39

GC content: 40.61

Gene sequence:

>1773_bases
ATGGAAAATCTAGCAAGCCTACCAGAAGTACAAAAGATCATCTCGATCGGAAAAGCGAATCGAGAAGTATCTTATGATGA
GATCAATGAAATACTACCGGATAAAATTCTAAATTCCGAAAAGATTGATGATGTCTTCACCTTGTTACACGAGATGGGGA
TTGAAATTGTCGAAGAATATTCTAAAAAATCTTTAGAAGAATCAAGTAGCCTCACGACTACCAAAGAAGAAACGACAAAA
GAAACAAAAGAAAAACCGGCACGTAAAAAAAGAGAGTCCAGTGTTTCTTCTAGTTCTGAAGATCCCATTCGTCTTTATTT
AAAAGAGATTGGTAAAGTTTCCCTTATCTCTGGTGAAACAGAGGTATTCCTTGCCAAACGGATTGAGAAGGGTGAAAAGA
TCATTGAAGAAACGATCTTAAGTTCCTCCATCCTTCGTCAAAACTTTGCCAAACTGATTCCAAAAATTAAGTCCAAAAAA
ATCAAAGTTTATGACTTGGTGAAAGTGGACAAAATGTACGCACTCAACCAAGAGCAAGCGGACAAATTGGAAAAAGTATT
TTTTGAGAATATGGAACTCATCCAACAAGATGAGAAGGTCCTAAACGAATCCACAAACCGCATTCGTAAGTATTCGGAAA
ATTCTAAGAAGTTCAAAGAACTCAAAGAAAAAATCGATTTATCCACTGGAAAAATTGATGAAGCCATTCGTAAAATTGGT
GTTTCCCAAAAAGAAATCCAAAAGATCTCTCAAAAGATCAAATCAATGGTTTTCCGTGTTAAGGAAATCGAAAAACACTT
CTTAAAAATCAAAGCCAAATACGGCCACGATGTTCGTGAAATCAAAGCTCTCAACCGTTTCATCGAAAAGAATGAAAACC
TAGATGAAATCGAAAAGATGATGGGTTGTGACATTGATGAAGTGAGAGAAGTCATCAAAGACATCCGCAATAATGAAAGA
AAACTCCGACGTATGGAGCAGGAAGCGGGTTCTCCTGTTGGGGAGATCAAAGATTGGGGTGAAAAGATCATCAAAGGGGA
AAGGGAAATTGCACAAGCCAAAAGAGAACTTGTACGAGCTAACCTTCGTTTGGTGGTATCGATTGCGAAACGATACGCTA
ACCGCGGTATGCATTTCTTTGACTTAATCCAAGAAGGAAACATCGGTCTCATTCGTGCGGTCGATAAATTCGAATACAAA
AAAGGGTATAAGTTTTCCACTTACGCCACTTGGTGGATCAGACAAGCCATCACTCGTGCGATCTCTGACCAAGCAAGGAC
CATTCGTGTACCGGTTCACATGATCGAACAAGTGAACAAAGTGATCCGGGAAACACGTCTCTTTGTCCAAGAGTTTGGCC
GTGACCCATCCAATGATGAAATTGCCGAACGACTCGGTTGGCCAGTGCAGAAAGTAAAGGCTGTGAAAAACGTGGCCCGC
GAACCAATTTCACTCGAGATCCCTGTGGGTTCGGAAGAAGATTCGGAACTCGGAGATTTTATTGAAGACAAAGAAGTGAT
CTCGCCACTCAACTCTGCCGCATCCTCGATCCTCTCTGAACAGATACGCCAAGTGTTACAAACACTGCCTGCTCGGGAAC
AAAAAGTGATTCGGATGCGATTTGGCTTGGATGATGGGTATGCACAAACACTTGAAGAAGTAGGTTACCAATTTAAGGTA
ACTCGTGAGAGGATTCGTCAGATCGAAGCAAAAGCACTTCGTAGACTCCGACACCCAAGTCGTTCCAAAAAATTGAAAGA
CTATATCGACTAA

Upstream 100 bases:

>100_bases
CTTTTGTCAGAACTTTCACTTTTAAAAAGTGAAAAAAATAAGAGATCCGTGTATCTCCGAACGATCCAAACGTTAGAAGT
CTAAAGAGGATATAGGTAGA

Downstream 100 bases:

>100_bases
AACAAATTTGTTTTTTACTTGATTGGACTTTCGAAGAGATTCATGGTTTGGAAAATCCATGGATCTCTTTTTATTTTGCC
ATCATACCTCTATTCCTTTG

Product: RNA polymerase sigma factor RpoD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 590; Mature: 590

Protein sequence:

>590_residues
MENLASLPEVQKIISIGKANREVSYDEINEILPDKILNSEKIDDVFTLLHEMGIEIVEEYSKKSLEESSSLTTTKEETTK
ETKEKPARKKRESSVSSSSEDPIRLYLKEIGKVSLISGETEVFLAKRIEKGEKIIEETILSSSILRQNFAKLIPKIKSKK
IKVYDLVKVDKMYALNQEQADKLEKVFFENMELIQQDEKVLNESTNRIRKYSENSKKFKELKEKIDLSTGKIDEAIRKIG
VSQKEIQKISQKIKSMVFRVKEIEKHFLKIKAKYGHDVREIKALNRFIEKNENLDEIEKMMGCDIDEVREVIKDIRNNER
KLRRMEQEAGSPVGEIKDWGEKIIKGEREIAQAKRELVRANLRLVVSIAKRYANRGMHFFDLIQEGNIGLIRAVDKFEYK
KGYKFSTYATWWIRQAITRAISDQARTIRVPVHMIEQVNKVIRETRLFVQEFGRDPSNDEIAERLGWPVQKVKAVKNVAR
EPISLEIPVGSEEDSELGDFIEDKEVISPLNSAASSILSEQIRQVLQTLPAREQKVIRMRFGLDDGYAQTLEEVGYQFKV
TRERIRQIEAKALRRLRHPSRSKKLKDYID

Sequences:

>Translated_590_residues
MENLASLPEVQKIISIGKANREVSYDEINEILPDKILNSEKIDDVFTLLHEMGIEIVEEYSKKSLEESSSLTTTKEETTK
ETKEKPARKKRESSVSSSSEDPIRLYLKEIGKVSLISGETEVFLAKRIEKGEKIIEETILSSSILRQNFAKLIPKIKSKK
IKVYDLVKVDKMYALNQEQADKLEKVFFENMELIQQDEKVLNESTNRIRKYSENSKKFKELKEKIDLSTGKIDEAIRKIG
VSQKEIQKISQKIKSMVFRVKEIEKHFLKIKAKYGHDVREIKALNRFIEKNENLDEIEKMMGCDIDEVREVIKDIRNNER
KLRRMEQEAGSPVGEIKDWGEKIIKGEREIAQAKRELVRANLRLVVSIAKRYANRGMHFFDLIQEGNIGLIRAVDKFEYK
KGYKFSTYATWWIRQAITRAISDQARTIRVPVHMIEQVNKVIRETRLFVQEFGRDPSNDEIAERLGWPVQKVKAVKNVAR
EPISLEIPVGSEEDSELGDFIEDKEVISPLNSAASSILSEQIRQVLQTLPAREQKVIRMRFGLDDGYAQTLEEVGYQFKV
TRERIRQIEAKALRRLRHPSRSKKLKDYID
>Mature_590_residues
MENLASLPEVQKIISIGKANREVSYDEINEILPDKILNSEKIDDVFTLLHEMGIEIVEEYSKKSLEESSSLTTTKEETTK
ETKEKPARKKRESSVSSSSEDPIRLYLKEIGKVSLISGETEVFLAKRIEKGEKIIEETILSSSILRQNFAKLIPKIKSKK
IKVYDLVKVDKMYALNQEQADKLEKVFFENMELIQQDEKVLNESTNRIRKYSENSKKFKELKEKIDLSTGKIDEAIRKIG
VSQKEIQKISQKIKSMVFRVKEIEKHFLKIKAKYGHDVREIKALNRFIEKNENLDEIEKMMGCDIDEVREVIKDIRNNER
KLRRMEQEAGSPVGEIKDWGEKIIKGEREIAQAKRELVRANLRLVVSIAKRYANRGMHFFDLIQEGNIGLIRAVDKFEYK
KGYKFSTYATWWIRQAITRAISDQARTIRVPVHMIEQVNKVIRETRLFVQEFGRDPSNDEIAERLGWPVQKVKAVKNVAR
EPISLEIPVGSEEDSELGDFIEDKEVISPLNSAASSILSEQIRQVLQTLPAREQKVIRMRFGLDDGYAQTLEEVGYQFKV
TRERIRQIEAKALRRLRHPSRSKKLKDYID

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=283, Percent_Identity=57.5971731448763, Blast_Score=344, Evalue=1e-95,
Organism=Escherichia coli, GI1789098, Length=233, Percent_Identity=42.4892703862661, Blast_Score=202, Evalue=5e-53,
Organism=Escherichia coli, GI1789871, Length=286, Percent_Identity=27.972027972028, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1788231, Length=239, Percent_Identity=28.0334728033473, Blast_Score=75, Evalue=8e-15,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR007127
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF03979 Sigma70_r1_1; PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 68548; Mature: 68548

Theoretical pI: Translated: 9.75; Mature: 9.75

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENLASLPEVQKIISIGKANREVSYDEINEILPDKILNSEKIDDVFTLLHEMGIEIVEEY
CCCHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
SKKSLEESSSLTTTKEETTKETKEKPARKKRESSVSSSSEDPIRLYLKEIGKVSLISGET
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCEEEECCCH
EVFLAKRIEKGEKIIEETILSSSILRQNFAKLIPKIKSKKIKVYDLVKVDKMYALNQEQA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHCCHHHH
DKLEKVFFENMELIQQDEKVLNESTNRIRKYSENSKKFKELKEKIDLSTGKIDEAIRKIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHC
VSQKEIQKISQKIKSMVFRVKEIEKHFLKIKAKYGHDVREIKALNRFIEKNENLDEIEKM
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHH
MGCDIDEVREVIKDIRNNERKLRRMEQEAGSPVGEIKDWGEKIIKGEREIAQAKRELVRA
HCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHH
NLRLVVSIAKRYANRGMHFFDLIQEGNIGLIRAVDKFEYKKGYKFSTYATWWIRQAITRA
HHHHHHHHHHHHHHCCCHHHHHHHCCCCCEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
ISDQARTIRVPVHMIEQVNKVIRETRLFVQEFGRDPSNDEIAERLGWPVQKVKAVKNVAR
HHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHC
EPISLEIPVGSEEDSELGDFIEDKEVISPLNSAASSILSEQIRQVLQTLPAREQKVIRMR
CCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
FGLDDGYAQTLEEVGYQFKVTRERIRQIEAKALRRLRHPSRSKKLKDYID
HCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCC
>Mature Secondary Structure
MENLASLPEVQKIISIGKANREVSYDEINEILPDKILNSEKIDDVFTLLHEMGIEIVEEY
CCCHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
SKKSLEESSSLTTTKEETTKETKEKPARKKRESSVSSSSEDPIRLYLKEIGKVSLISGET
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCEEEECCCH
EVFLAKRIEKGEKIIEETILSSSILRQNFAKLIPKIKSKKIKVYDLVKVDKMYALNQEQA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHCCHHHH
DKLEKVFFENMELIQQDEKVLNESTNRIRKYSENSKKFKELKEKIDLSTGKIDEAIRKIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHC
VSQKEIQKISQKIKSMVFRVKEIEKHFLKIKAKYGHDVREIKALNRFIEKNENLDEIEKM
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHH
MGCDIDEVREVIKDIRNNERKLRRMEQEAGSPVGEIKDWGEKIIKGEREIAQAKRELVRA
HCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHH
NLRLVVSIAKRYANRGMHFFDLIQEGNIGLIRAVDKFEYKKGYKFSTYATWWIRQAITRA
HHHHHHHHHHHHHHCCCHHHHHHHCCCCCEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
ISDQARTIRVPVHMIEQVNKVIRETRLFVQEFGRDPSNDEIAERLGWPVQKVKAVKNVAR
HHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHC
EPISLEIPVGSEEDSELGDFIEDKEVISPLNSAASSILSEQIRQVLQTLPAREQKVIRMR
CCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
FGLDDGYAQTLEEVGYQFKVTRERIRQIEAKALRRLRHPSRSKKLKDYID
HCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA