Click here to switch to the map view.

The map label for this gene is dnaG [H]

Identifier: 183220953

GI number: 183220953

Start: 1632050

End: 1633861

Strand: Direct

Name: dnaG [H]

Synonym: LEPBI_I1566

Alternate gene names: 183220953

Gene position: 1632050-1633861 (Clockwise)

Preceding gene: 183220952

Following gene: 183220954

Centisome position: 45.34

GC content: 41.5

Gene sequence:

>1812_bases
GTGAATCCTTACCAAAGTTTTAAAGAAAGAGTTCGCAGAGAAGTCTCCATTGACTCCTACATCAACCGATTTGTTCCGTT
ACGCCGAATGGGTAGAAATCTCGTTGGCATATGCCCTTTTCATAATGAAAAAACCCCATCATTCAATGTAAACCCAGAAG
GTGGATTTTACCACTGTTTTGGATGTAAGGCATCGGGTGATTTATTCCGCTTTGTGATGGATTACCAAAAAGTGGATTTT
TTAAAATCTCTGGAAGTCCTCTCTGAATATTCAGGCATTCCACTCGTGGAAAGAACCAAAGAAGAAGAAGAGTCGGAACG
AAAAAAAGAAGCACTTTATCAAATTTCACAAAAAGCACTCGAATACTTTCAAAAAAATCTAAATACAACTGCCGGTGAAC
TTGCGTTAAAGTATTTAGAATCACGTGGTATGTATGCCGAAGACCTAAAGGTTTTTAAGATTGGATTTGGACTTCCTGGA
TTTGGAAATTTACGAAGCGAGTTGTTCAAAACAGATGCGGAAGTGAAGTTAGGAGAACAGTTGGGTCTACTCAAACGACA
GGACCAAAACAAAGATCCTTATGATTTTTTTCGCAACCGGATCATGTTCCCTGTGATTGATCCAAGAGGAAGAGTGGTTG
CCTTTTCGGGACGTATCTTAGGTGAATCGGAAGAAGCCAAATACATCAATAGCCCAAACTCACTCATTTACGACAAAAGC
CGTACGTTTTATAATTTAAATTTAGCCCAAGACAGCATACGCAAAACACGAGAAGCTGTCATCGTGGAAGGTGTTTTCGA
CGCCATTGGTCTCTTTCGCAAAGGAATTGAATTTGTCGTCGCACCTCTTGGTACTGGCTTCACAGAAGGCCATGTTCGGA
TTTTAAAAAACATGGCAGACAAAGTGTATCTGATGATGGATTCCGATAAAGCTGGAACCAAAGGTGCCTTTCGTGCCGTA
AACCTTCTCTCCAAGGAAGGAGTTGTGGTAAAAGTTTGCCATATCCCCGAAGGCAAAGACCCATTTGATTATTCCCTCCA
TCATAACAAACAGGAAATCCGAGAATTACTGGAAGGTGCCTCTCCCGCTTCTCAATTTATGATCCGAGAGATCCTCGCTG
GGGCAGGTCCGACTTCCCTTGCAGAAGAAAAACAAGCAGGAGTGAAAAAACTCTTTGAATTCTTAAAACCAATGGAGAAG
GAAACGGACAAACAGGTCTATTTAGAAGAGGGAGCTCGCCAACTGGGACTCTCATTTTCTTCTCTTTTTCAGGACTTTCG
TGGGAAACCAGGTGTAACTTCGACCCCTTCCGTTGTCGATACTAAGAAAGACCGCACGCCACAGAAACCGGGGAAACCAT
CTCCTGTTCTTGTCTGTGAGCGGAAGATGATAGCCATGCTCATTCAAAACTTAGAACTTTTTAGTTTTGCTGATGACTTA
CTTTCACTTGAGTTTCGTGATGAGGTGTCTGCCTTTTTTTGGGACTATTTATATACAAAGTATTTGCAGAATGAGAACCT
AACAGCTGCAGAAATTCTTTCGAGGGAAGAAATTCCTTCGGAATACCTGGGAATGATTGCTGAACATTTTTCGGCCGATG
TATCAGTGACCCCAGCTACATTTAGAGCGATGTTTCTTTACCATGCGGATTTGTTGGATGACGCACGGATGGAAGAACTT
GTGAAAGAAATGGCGAAACCTGACTTAACGATTGAAGAGAAGAACAATCTTTTGTCAGAACTTTCACTTTTAAAAAGTGA
AAAAAATAAGAGATCCGTGTATCTCCGAACGATCCAAACGTTAGAAGTCTAA

Upstream 100 bases:

>100_bases
GGGTAAAGTAATGGCAAAATTTAAAGGACAAAATATAGACGGCTCCAAGGTATCCTCCCTCGTTAAACAAGCACTTAGCG
CCCGTTAAAATAACCAAACT

Downstream 100 bases:

>100_bases
AGAGGATATAGGTAGAATGGAAAATCTAGCAAGCCTACCAGAAGTACAAAAGATCATCTCGATCGGAAAAGCGAATCGAG
AAGTATCTTATGATGAGATC

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 603; Mature: 603

Protein sequence:

>603_residues
MNPYQSFKERVRREVSIDSYINRFVPLRRMGRNLVGICPFHNEKTPSFNVNPEGGFYHCFGCKASGDLFRFVMDYQKVDF
LKSLEVLSEYSGIPLVERTKEEEESERKKEALYQISQKALEYFQKNLNTTAGELALKYLESRGMYAEDLKVFKIGFGLPG
FGNLRSELFKTDAEVKLGEQLGLLKRQDQNKDPYDFFRNRIMFPVIDPRGRVVAFSGRILGESEEAKYINSPNSLIYDKS
RTFYNLNLAQDSIRKTREAVIVEGVFDAIGLFRKGIEFVVAPLGTGFTEGHVRILKNMADKVYLMMDSDKAGTKGAFRAV
NLLSKEGVVVKVCHIPEGKDPFDYSLHHNKQEIRELLEGASPASQFMIREILAGAGPTSLAEEKQAGVKKLFEFLKPMEK
ETDKQVYLEEGARQLGLSFSSLFQDFRGKPGVTSTPSVVDTKKDRTPQKPGKPSPVLVCERKMIAMLIQNLELFSFADDL
LSLEFRDEVSAFFWDYLYTKYLQNENLTAAEILSREEIPSEYLGMIAEHFSADVSVTPATFRAMFLYHADLLDDARMEEL
VKEMAKPDLTIEEKNNLLSELSLLKSEKNKRSVYLRTIQTLEV

Sequences:

>Translated_603_residues
MNPYQSFKERVRREVSIDSYINRFVPLRRMGRNLVGICPFHNEKTPSFNVNPEGGFYHCFGCKASGDLFRFVMDYQKVDF
LKSLEVLSEYSGIPLVERTKEEEESERKKEALYQISQKALEYFQKNLNTTAGELALKYLESRGMYAEDLKVFKIGFGLPG
FGNLRSELFKTDAEVKLGEQLGLLKRQDQNKDPYDFFRNRIMFPVIDPRGRVVAFSGRILGESEEAKYINSPNSLIYDKS
RTFYNLNLAQDSIRKTREAVIVEGVFDAIGLFRKGIEFVVAPLGTGFTEGHVRILKNMADKVYLMMDSDKAGTKGAFRAV
NLLSKEGVVVKVCHIPEGKDPFDYSLHHNKQEIRELLEGASPASQFMIREILAGAGPTSLAEEKQAGVKKLFEFLKPMEK
ETDKQVYLEEGARQLGLSFSSLFQDFRGKPGVTSTPSVVDTKKDRTPQKPGKPSPVLVCERKMIAMLIQNLELFSFADDL
LSLEFRDEVSAFFWDYLYTKYLQNENLTAAEILSREEIPSEYLGMIAEHFSADVSVTPATFRAMFLYHADLLDDARMEEL
VKEMAKPDLTIEEKNNLLSELSLLKSEKNKRSVYLRTIQTLEV
>Mature_603_residues
MNPYQSFKERVRREVSIDSYINRFVPLRRMGRNLVGICPFHNEKTPSFNVNPEGGFYHCFGCKASGDLFRFVMDYQKVDF
LKSLEVLSEYSGIPLVERTKEEEESERKKEALYQISQKALEYFQKNLNTTAGELALKYLESRGMYAEDLKVFKIGFGLPG
FGNLRSELFKTDAEVKLGEQLGLLKRQDQNKDPYDFFRNRIMFPVIDPRGRVVAFSGRILGESEEAKYINSPNSLIYDKS
RTFYNLNLAQDSIRKTREAVIVEGVFDAIGLFRKGIEFVVAPLGTGFTEGHVRILKNMADKVYLMMDSDKAGTKGAFRAV
NLLSKEGVVVKVCHIPEGKDPFDYSLHHNKQEIRELLEGASPASQFMIREILAGAGPTSLAEEKQAGVKKLFEFLKPMEK
ETDKQVYLEEGARQLGLSFSSLFQDFRGKPGVTSTPSVVDTKKDRTPQKPGKPSPVLVCERKMIAMLIQNLELFSFADDL
LSLEFRDEVSAFFWDYLYTKYLQNENLTAAEILSREEIPSEYLGMIAEHFSADVSVTPATFRAMFLYHADLLDDARMEEL
VKEMAKPDLTIEEKNNLLSELSLLKSEKNKRSVYLRTIQTLEV

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=578, Percent_Identity=28.8927335640138, Blast_Score=235, Evalue=8e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007693
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 68789; Mature: 68789

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPYQSFKERVRREVSIDSYINRFVPLRRMGRNLVGICPFHNEKTPSFNVNPEGGFYHCF
CCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCEEEEE
GCKASGDLFRFVMDYQKVDFLKSLEVLSEYSGIPLVERTKEEEESERKKEALYQISQKAL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
EYFQKNLNTTAGELALKYLESRGMYAEDLKVFKIGFGLPGFGNLRSELFKTDAEVKLGEQ
HHHHHHCCCHHHHHHHHHHHHCCCCHHCCEEEEEECCCCCCHHHHHHHHHCCCCHHHHHH
LGLLKRQDQNKDPYDFFRNRIMFPVIDPRGRVVAFSGRILGESEEAKYINSPNSLIYDKS
HHHHHHCCCCCCHHHHHHCCEEEEEECCCCCEEEEECCEECCCCCCHHCCCCCCEEEECC
RTFYNLNLAQDSIRKTREAVIVEGVFDAIGLFRKGIEFVVAPLGTGFTEGHVRILKNMAD
CEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCHHEEEECCCCCCCHHHHHHHHHHHC
KVYLMMDSDKAGTKGAFRAVNLLSKEGVVVKVCHIPEGKDPFDYSLHHNKQEIRELLEGA
EEEEEEECCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHCC
SPASQFMIREILAGAGPTSLAEEKQAGVKKLFEFLKPMEKETDKQVYLEEGARQLGLSFS
CHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHH
SLFQDFRGKPGVTSTPSVVDTKKDRTPQKPGKPSPVLVCERKMIAMLIQNLELFSFADDL
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHH
LSLEFRDEVSAFFWDYLYTKYLQNENLTAAEILSREEIPSEYLGMIAEHFSADVSVTPAT
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCEECHHH
FRAMFLYHADLLDDARMEELVKEMAKPDLTIEEKNNLLSELSLLKSEKNKRSVYLRTIQT
HHHHHHHHHHHHCHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
LEV
HCC
>Mature Secondary Structure
MNPYQSFKERVRREVSIDSYINRFVPLRRMGRNLVGICPFHNEKTPSFNVNPEGGFYHCF
CCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCEEEEE
GCKASGDLFRFVMDYQKVDFLKSLEVLSEYSGIPLVERTKEEEESERKKEALYQISQKAL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
EYFQKNLNTTAGELALKYLESRGMYAEDLKVFKIGFGLPGFGNLRSELFKTDAEVKLGEQ
HHHHHHCCCHHHHHHHHHHHHCCCCHHCCEEEEEECCCCCCHHHHHHHHHCCCCHHHHHH
LGLLKRQDQNKDPYDFFRNRIMFPVIDPRGRVVAFSGRILGESEEAKYINSPNSLIYDKS
HHHHHHCCCCCCHHHHHHCCEEEEEECCCCCEEEEECCEECCCCCCHHCCCCCCEEEECC
RTFYNLNLAQDSIRKTREAVIVEGVFDAIGLFRKGIEFVVAPLGTGFTEGHVRILKNMAD
CEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCHHEEEECCCCCCCHHHHHHHHHHHC
KVYLMMDSDKAGTKGAFRAVNLLSKEGVVVKVCHIPEGKDPFDYSLHHNKQEIRELLEGA
EEEEEEECCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHCC
SPASQFMIREILAGAGPTSLAEEKQAGVKKLFEFLKPMEKETDKQVYLEEGARQLGLSFS
CHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHH
SLFQDFRGKPGVTSTPSVVDTKKDRTPQKPGKPSPVLVCERKMIAMLIQNLELFSFADDL
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHH
LSLEFRDEVSAFFWDYLYTKYLQNENLTAAEILSREEIPSEYLGMIAEHFSADVSVTPAT
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCEECHHH
FRAMFLYHADLLDDARMEELVKEMAKPDLTIEEKNNLLSELSLLKSEKNKRSVYLRTIQT
HHHHHHHHHHHHCHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
LEV
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]