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The map label for this gene is acoC [H]

Identifier: 183220944

GI number: 183220944

Start: 1624148

End: 1625542

Strand: Reverse

Name: acoC [H]

Synonym: LEPBI_I1557

Alternate gene names: 183220944

Gene position: 1625542-1624148 (Counterclockwise)

Preceding gene: 183220945

Following gene: 183220942

Centisome position: 45.16

GC content: 45.09

Gene sequence:

>1395_bases
ATGATGGCAAAAATCCAAGAAATGACCCAATTATCTCCTACCATGGAAGAAGGAACCATTGTTAAGTGGTTGAAAAATGA
AGGGGACGCAATCAGTCCAGGTGACATCCTAGCAGAAGTCGAAACCGATAAAGCTGTGATGGAGATGGAAGCCTATGATG
CAGGTGTGATCCTTAAAATCATCCAACAGGAAGGCACCAAACTGAGAGTTGGTGAAGCCATGGCAATCATTGGAAAACCT
GGAGAAGACATCTCCTCCCTCCTTTCCAATCTTCCGAAAAAAGAAACGCAGGCAAAAGGCCCAAATCCCACTAGTCCCTC
CCCATCCGGGGAAGTAAGTTCCGAATCGGGAGCTAATGGAAATGATCGAGCTATCACCCAAGAAAAAGCCATACAAGAAA
AACAAACCCTAGAAACACCTACCGAAGCCCAATTTCCAGTGGCTCAAGGATCAGCCATTCCTACAAAGAATTCAAGTTCG
TCGCAAAAACCTCTGCCTCTTTCCAACATTCCAAGAGGATCCCTCCGTGTACTTGCCTCTCCTCTTGCCAAATCCATCGC
CATCGAACATGGAATCGATTTGCACCAAGTTTTGGGAACTGGCCCGGAAGGTAGGATCACCAAAAAAGATGTTCTGGATT
CCTTAAACAAAGGTTTTCGTCCATCGAGTTTTGATTCTGACAAGATGAGCCAAGATGAAGTGGTCAGTTTGAATGGAATG
CGGAAAACCATTGCCAAACGACTCACAGAATCCAAACAGAACCTCCCCCATTTTTATTTGAATGTGGATATAAATGCCAA
AGCACTGGAGTCCTTCCGAAAGGAAATTAACGACTTCCAAACCTCACAAAATCCAGATTCGGCAAATAAGGTGAGTTTGA
ATGACATCATTGTCAAAGCAACCGCGGCCGCACTCAAACTCCACCCAAAAGTCAATGCGAGTTTCCAAGGAGATTCTATC
TTACAATTTGGGAGAGTGGATGTAGGGATTGCTGTCTCCATTGATGGCGGTCTATTAACGCCTGTCATTCGAGATGCGAA
TCGAAAGTCCATCTTAGAAATTTCAAAAGAAGTGAAGGAACTCGCCAAAAAAGCAAGGGATCGAAAGTTAAAACCGGAAG
AATTTACCAATGGAACCTTTACCATTTCCAATTTAGGGATGTATGGGATCAGTCGGTTTACGGCGATCATCAACGAACCG
GAAGGAGCCATCCTGGCTGTAGGTTCTGCAGAAGACAAACCGGTTGTGGAAAACGGAGTTGTGGTAGCGGGAAGGGTAAT
TTCCCTCACACTCTCCTGCGATCATCGTGTGATCGATGGAGCTGTGGGAGCAGAGTTTCTAAAGACCTTAAGGAGCTTTT
TGGAAAAACCAAACCTACTCCTTGCAGTCGGTTAA

Upstream 100 bases:

>100_bases
GATGTTCCTATGTCTTACGCTGCCAACTTAGAAAAAATGAGTTTGCCGAGTGCCAATCGAGTGGCTGATACCATCCGCGA
GATGTTACGTTAAGGAGACC

Downstream 100 bases:

>100_bases
AGATTCTTATGGATTAAGCTGCAAAAAAATCTGCACTTTGGATGTCGGCAATGGGGATACGAACCACACCTGTTGCCAAA
TTTCCGATGACCACATCGTC

Product: pyruvate dehydrogenase complex dihydrolipoyllysine-residue acetyltransferase

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MMAKIQEMTQLSPTMEEGTIVKWLKNEGDAISPGDILAEVETDKAVMEMEAYDAGVILKIIQQEGTKLRVGEAMAIIGKP
GEDISSLLSNLPKKETQAKGPNPTSPSPSGEVSSESGANGNDRAITQEKAIQEKQTLETPTEAQFPVAQGSAIPTKNSSS
SQKPLPLSNIPRGSLRVLASPLAKSIAIEHGIDLHQVLGTGPEGRITKKDVLDSLNKGFRPSSFDSDKMSQDEVVSLNGM
RKTIAKRLTESKQNLPHFYLNVDINAKALESFRKEINDFQTSQNPDSANKVSLNDIIVKATAAALKLHPKVNASFQGDSI
LQFGRVDVGIAVSIDGGLLTPVIRDANRKSILEISKEVKELAKKARDRKLKPEEFTNGTFTISNLGMYGISRFTAIINEP
EGAILAVGSAEDKPVVENGVVVAGRVISLTLSCDHRVIDGAVGAEFLKTLRSFLEKPNLLLAVG

Sequences:

>Translated_464_residues
MMAKIQEMTQLSPTMEEGTIVKWLKNEGDAISPGDILAEVETDKAVMEMEAYDAGVILKIIQQEGTKLRVGEAMAIIGKP
GEDISSLLSNLPKKETQAKGPNPTSPSPSGEVSSESGANGNDRAITQEKAIQEKQTLETPTEAQFPVAQGSAIPTKNSSS
SQKPLPLSNIPRGSLRVLASPLAKSIAIEHGIDLHQVLGTGPEGRITKKDVLDSLNKGFRPSSFDSDKMSQDEVVSLNGM
RKTIAKRLTESKQNLPHFYLNVDINAKALESFRKEINDFQTSQNPDSANKVSLNDIIVKATAAALKLHPKVNASFQGDSI
LQFGRVDVGIAVSIDGGLLTPVIRDANRKSILEISKEVKELAKKARDRKLKPEEFTNGTFTISNLGMYGISRFTAIINEP
EGAILAVGSAEDKPVVENGVVVAGRVISLTLSCDHRVIDGAVGAEFLKTLRSFLEKPNLLLAVG
>Mature_464_residues
MMAKIQEMTQLSPTMEEGTIVKWLKNEGDAISPGDILAEVETDKAVMEMEAYDAGVILKIIQQEGTKLRVGEAMAIIGKP
GEDISSLLSNLPKKETQAKGPNPTSPSPSGEVSSESGANGNDRAITQEKAIQEKQTLETPTEAQFPVAQGSAIPTKNSSS
SQKPLPLSNIPRGSLRVLASPLAKSIAIEHGIDLHQVLGTGPEGRITKKDVLDSLNKGFRPSSFDSDKMSQDEVVSLNGM
RKTIAKRLTESKQNLPHFYLNVDINAKALESFRKEINDFQTSQNPDSANKVSLNDIIVKATAAALKLHPKVNASFQGDSI
LQFGRVDVGIAVSIDGGLLTPVIRDANRKSILEISKEVKELAKKARDRKLKPEEFTNGTFTISNLGMYGISRFTAIINEP
EGAILAVGSAEDKPVVENGVVVAGRVISLTLSCDHRVIDGAVGAEFLKTLRSFLEKPNLLLAVG

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=473, Percent_Identity=38.9006342494715, Blast_Score=288, Evalue=6e-78,
Organism=Homo sapiens, GI203098816, Length=503, Percent_Identity=34.5924453280318, Blast_Score=246, Evalue=3e-65,
Organism=Homo sapiens, GI203098753, Length=503, Percent_Identity=34.5924453280318, Blast_Score=245, Evalue=5e-65,
Organism=Homo sapiens, GI260898739, Length=156, Percent_Identity=46.1538461538462, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI110671329, Length=314, Percent_Identity=28.6624203821656, Blast_Score=122, Evalue=9e-28,
Organism=Homo sapiens, GI19923748, Length=228, Percent_Identity=32.8947368421053, Blast_Score=117, Evalue=3e-26,
Organism=Escherichia coli, GI1786946, Length=461, Percent_Identity=30.8026030368764, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI1786305, Length=298, Percent_Identity=33.8926174496644, Blast_Score=153, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17560088, Length=454, Percent_Identity=39.8678414096916, Blast_Score=285, Evalue=3e-77,
Organism=Caenorhabditis elegans, GI17538894, Length=311, Percent_Identity=34.7266881028939, Blast_Score=165, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI17537937, Length=335, Percent_Identity=29.2537313432836, Blast_Score=156, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI25146366, Length=451, Percent_Identity=28.159645232816, Blast_Score=142, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6324258, Length=484, Percent_Identity=34.9173553719008, Blast_Score=235, Evalue=1e-62,
Organism=Saccharomyces cerevisiae, GI6320352, Length=227, Percent_Identity=37.0044052863436, Blast_Score=147, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6321632, Length=219, Percent_Identity=30.1369863013699, Blast_Score=78, Evalue=2e-15,
Organism=Drosophila melanogaster, GI20129315, Length=298, Percent_Identity=39.9328859060403, Blast_Score=204, Evalue=1e-52,
Organism=Drosophila melanogaster, GI24582497, Length=298, Percent_Identity=39.9328859060403, Blast_Score=203, Evalue=2e-52,
Organism=Drosophila melanogaster, GI18859875, Length=467, Percent_Identity=28.9079229122056, Blast_Score=156, Evalue=2e-38,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006257
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 49884; Mature: 49884

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMAKIQEMTQLSPTMEEGTIVKWLKNEGDAISPGDILAEVETDKAVMEMEAYDAGVILKI
CCCHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCEEEEECCCHHHHHHHHCCCHHHHHH
IQQEGTKLRVGEAMAIIGKPGEDISSLLSNLPKKETQAKGPNPTSPSPSGEVSSESGANG
HHHCCCEEEECCEEEEECCCCHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCC
NDRAITQEKAIQEKQTLETPTEAQFPVAQGSAIPTKNSSSSQKPLPLSNIPRGSLRVLAS
CCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
PLAKSIAIEHGIDLHQVLGTGPEGRITKKDVLDSLNKGFRPSSFDSDKMSQDEVVSLNGM
HHHHHHHHHCCCCHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEEHHHH
RKTIAKRLTESKQNLPHFYLNVDINAKALESFRKEINDFQTSQNPDSANKVSLNDIIVKA
HHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHH
TAAALKLHPKVNASFQGDSILQFGRVDVGIAVSIDGGLLTPVIRDANRKSILEISKEVKE
HHHHEEECCCCCCCCCCCCHHEECCEEEEEEEEECCCCHHHHHHCCCHHHHHHHHHHHHH
LAKKARDRKLKPEEFTNGTFTISNLGMYGISRFTAIINEPEGAILAVGSAEDKPVVENGV
HHHHHHCCCCCCHHCCCCEEEEECCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCE
VVAGRVISLTLSCDHRVIDGAVGAEFLKTLRSFLEKPNLLLAVG
EEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEC
>Mature Secondary Structure
MMAKIQEMTQLSPTMEEGTIVKWLKNEGDAISPGDILAEVETDKAVMEMEAYDAGVILKI
CCCHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCEEEEECCCHHHHHHHHCCCHHHHHH
IQQEGTKLRVGEAMAIIGKPGEDISSLLSNLPKKETQAKGPNPTSPSPSGEVSSESGANG
HHHCCCEEEECCEEEEECCCCHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCC
NDRAITQEKAIQEKQTLETPTEAQFPVAQGSAIPTKNSSSSQKPLPLSNIPRGSLRVLAS
CCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
PLAKSIAIEHGIDLHQVLGTGPEGRITKKDVLDSLNKGFRPSSFDSDKMSQDEVVSLNGM
HHHHHHHHHCCCCHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEEHHHH
RKTIAKRLTESKQNLPHFYLNVDINAKALESFRKEINDFQTSQNPDSANKVSLNDIIVKA
HHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHH
TAAALKLHPKVNASFQGDSILQFGRVDVGIAVSIDGGLLTPVIRDANRKSILEISKEVKE
HHHHEEECCCCCCCCCCCCHHEECCEEEEEEEEECCCCHHHHHHCCCHHHHHHHHHHHHH
LAKKARDRKLKPEEFTNGTFTISNLGMYGISRFTAIINEPEGAILAVGSAEDKPVVENGV
HHHHHHCCCCCCHHCCCCEEEEECCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCE
VVAGRVISLTLSCDHRVIDGAVGAEFLKTLRSFLEKPNLLLAVG
EEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9515924 [H]