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The map label for this gene is truB [H]

Identifier: 183220914

GI number: 183220914

Start: 1592973

End: 1593902

Strand: Direct

Name: truB [H]

Synonym: LEPBI_I1527

Alternate gene names: 183220914

Gene position: 1592973-1593902 (Clockwise)

Preceding gene: 183220913

Following gene: 183220915

Centisome position: 44.25

GC content: 46.99

Gene sequence:

>930_bases
ATGTCTAAATCCGATCAGTCTGGATTCTTATTTGTATACAAACCCCCTGGCATCACTAGTTCTGATTTGGTTTTAAAAAC
CAAACGCCTGCTTGGTCAAAAATCAGTTGGCCACACCGGAACCTTGGACCGATTTGCGGAGGGCCTTCTCGTTTTACCGT
GTGGGGACTACACAACCTTTTCCCAAGTGTTTCTTGGAAAAGACAAAACCTATTTAGCAGAAGTGGTTGTGGGACTACGC
ACCGATTCTGGTGATCCTGATGGTGTGGTGGAAATTGAGACGAGAGAAAGTTCGCGAGTCGAATTTGTTTCAAGGTTTCC
CACGGAAAGACTGGAGACGGAACTGAAATCTCTCACAGAACAAAAAACCCAAAAAGCACCCAAAATTTCGGCTCTGAAAG
TAGGGGGCAAACGACAGTCTGATCTTTTCCGAGAAGGGACATTCGTGGAAGAGAAGGAACGAAACATCACCATCCATTCG
GTGGTAGACATCCAAAGGACGGAGACTGGATTTTCCTTTCGCATCCATGTGAGTTCAGGGACCTACATCCGTAAGATCAT
CCTTGATCTTTCCGACAAATGGGGGATCCCCCTTTCATTGGGGAGGCTTGTGCGGGAATCCATTGGAGAATACGGATTGG
ACGGAGTGAAACCCCTAGAAGAGATCACCAAAGCCGACCTTCGTACCTGGAAAGAAGTGTTCCCCTTACCTGTCCGGATC
GTGGACGAAGCGGAAAGAAAAGCAGTGGTCCACGGGGGATACATCTGGGACAAACTCCCGAAGGCAGATTCCCTTGGGTT
TTACATTCTCGATTCGGATGAAACCACCATCCTTGCCTGGTGTTCGTATGAAGAGAAACCGGCCCACATTCCTTACCGTT
ACCGAAAAGTATTTTTTGACCCTTCTGCAAAAATTATGTTTTCTAAATGA

Upstream 100 bases:

>100_bases
ATTCTGAAAGTGGAATCGAAAGAGAAACGTAAGGAAACCGAAACGAAAGTTTCCATTGCGAGTGTAAATTCTTCCATTCT
CTTTTGTTAGGTGATTAGAT

Downstream 100 bases:

>100_bases
GTCCTTCTTAAAATCTGGACACTAGTATGATCACAAAAGAACAAAAACAGCAGATCATTGCTACATTCGGTAGCAAACCA
AACGACACAGGTTCTGCAGA

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 309; Mature: 308

Protein sequence:

>309_residues
MSKSDQSGFLFVYKPPGITSSDLVLKTKRLLGQKSVGHTGTLDRFAEGLLVLPCGDYTTFSQVFLGKDKTYLAEVVVGLR
TDSGDPDGVVEIETRESSRVEFVSRFPTERLETELKSLTEQKTQKAPKISALKVGGKRQSDLFREGTFVEEKERNITIHS
VVDIQRTETGFSFRIHVSSGTYIRKIILDLSDKWGIPLSLGRLVRESIGEYGLDGVKPLEEITKADLRTWKEVFPLPVRI
VDEAERKAVVHGGYIWDKLPKADSLGFYILDSDETTILAWCSYEEKPAHIPYRYRKVFFDPSAKIMFSK

Sequences:

>Translated_309_residues
MSKSDQSGFLFVYKPPGITSSDLVLKTKRLLGQKSVGHTGTLDRFAEGLLVLPCGDYTTFSQVFLGKDKTYLAEVVVGLR
TDSGDPDGVVEIETRESSRVEFVSRFPTERLETELKSLTEQKTQKAPKISALKVGGKRQSDLFREGTFVEEKERNITIHS
VVDIQRTETGFSFRIHVSSGTYIRKIILDLSDKWGIPLSLGRLVRESIGEYGLDGVKPLEEITKADLRTWKEVFPLPVRI
VDEAERKAVVHGGYIWDKLPKADSLGFYILDSDETTILAWCSYEEKPAHIPYRYRKVFFDPSAKIMFSK
>Mature_308_residues
SKSDQSGFLFVYKPPGITSSDLVLKTKRLLGQKSVGHTGTLDRFAEGLLVLPCGDYTTFSQVFLGKDKTYLAEVVVGLRT
DSGDPDGVVEIETRESSRVEFVSRFPTERLETELKSLTEQKTQKAPKISALKVGGKRQSDLFREGTFVEEKERNITIHSV
VDIQRTETGFSFRIHVSSGTYIRKIILDLSDKWGIPLSLGRLVRESIGEYGLDGVKPLEEITKADLRTWKEVFPLPVRIV
DEAERKAVVHGGYIWDKLPKADSLGFYILDSDETTILAWCSYEEKPAHIPYRYRKVFFDPSAKIMFSK

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=225, Percent_Identity=29.3333333333333, Blast_Score=72, Evalue=5e-13,
Organism=Escherichia coli, GI2367200, Length=218, Percent_Identity=34.4036697247706, Blast_Score=115, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780 [H]

Pfam domain/function: PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 34923; Mature: 34792

Theoretical pI: Translated: 8.18; Mature: 8.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKSDQSGFLFVYKPPGITSSDLVLKTKRLLGQKSVGHTGTLDRFAEGLLVLPCGDYTTF
CCCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHCCEEEEECCCCCHH
SQVFLGKDKTYLAEVVVGLRTDSGDPDGVVEIETRESSRVEFVSRFPTERLETELKSLTE
HHHHCCCCHHHHHHHHHHEECCCCCCCCEEEEECCCCCHHHHHHHCCHHHHHHHHHHHHH
QKTQKAPKISALKVGGKRQSDLFREGTFVEEKERNITIHSVVDIQRTETGFSFRIHVSSG
HHHHCCCCEEEEEECCCCHHHHHHCCCCCCHHCCCEEEEEEEEEEECCCCEEEEEEECCC
TYIRKIILDLSDKWGIPLSLGRLVRESIGEYGLDGVKPLEEITKADLRTWKEVFPLPVRI
CEEEHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCEEE
VDEAERKAVVHGGYIWDKLPKADSLGFYILDSDETTILAWCSYEEKPAHIPYRYRKVFFD
ECCCCCCEEEECCCHHHCCCCCCCCEEEEECCCCEEEEEEECCCCCCCCCCEEEEEEEEC
PSAKIMFSK
CCCEEEECC
>Mature Secondary Structure 
SKSDQSGFLFVYKPPGITSSDLVLKTKRLLGQKSVGHTGTLDRFAEGLLVLPCGDYTTF
CCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHCCEEEEECCCCCHH
SQVFLGKDKTYLAEVVVGLRTDSGDPDGVVEIETRESSRVEFVSRFPTERLETELKSLTE
HHHHCCCCHHHHHHHHHHEECCCCCCCCEEEEECCCCCHHHHHHHCCHHHHHHHHHHHHH
QKTQKAPKISALKVGGKRQSDLFREGTFVEEKERNITIHSVVDIQRTETGFSFRIHVSSG
HHHHCCCCEEEEEECCCCHHHHHHCCCCCCHHCCCEEEEEEEEEEECCCCEEEEEEECCC
TYIRKIILDLSDKWGIPLSLGRLVRESIGEYGLDGVKPLEEITKADLRTWKEVFPLPVRI
CEEEHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCEEE
VDEAERKAVVHGGYIWDKLPKADSLGFYILDSDETTILAWCSYEEKPAHIPYRYRKVFFD
ECCCCCCEEEECCCHHHCCCCCCCCEEEEECCCCEEEEEEECCCCCCCCCCEEEEEEEEC
PSAKIMFSK
CCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA