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The map label for this gene is rbfA

Identifier: 183220913

GI number: 183220913

Start: 1592455

End: 1592904

Strand: Direct

Name: rbfA

Synonym: LEPBI_I1526

Alternate gene names: 183220913

Gene position: 1592455-1592904 (Clockwise)

Preceding gene: 183220912

Following gene: 183220914

Centisome position: 44.24

GC content: 40.89

Gene sequence:

>450_bases
ATGAATCCCATTCGAATGAAAAAACTCGAATCGGAGATCATTCGCCAAATCTCCACTGCGATTTTAGAAGGAAAGGTAAA
AGACCCTCGGGTCTTTTTGCCTAGTTTCCACCGCATCGAAATTAGCGAAGACTTGAAGTATGCGAAGGTATACTTCACGG
CTCTTTGTAATAATAATGAGAGAAAAAAACTCACACAAGGGCTTGTTTCTTGTGCGGGGTTTTTATCTTCATTTGTTGGA
AAAAACCTACGCTTGCATACCAATCCGAAATTTACCTTTGTCTGGGACAACAGCTACATCAAAAGTTTAGAAGTGAATCG
CCTCATCGATGATTCCGCTCCCAAAACTTTATTTGAAGAGCTCCACCCAAATCCTGAAGAGGATGACGGGGACACAGATG
CAGAAACTTTGTTAGAAGATTCTGAAAGTGGAATCGAAAGAGAAACGTAA

Upstream 100 bases:

>100_bases
TGTGGAATCTTACTCGATGGATTCAATGACTTCTCTGTGGGTGATGAGATCGAAGCATACGAGATTCGCGAGATTGCTCG
TAAACTATAAGGCGGCCTAA

Downstream 100 bases:

>100_bases
GGAAACCGAAACGAAAGTTTCCATTGCGAGTGTAAATTCTTCCATTCTCTTTTGTTAGGTGATTAGATATGTCTAAATCC
GATCAGTCTGGATTCTTATT

Product: ribosome-binding factor A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 149; Mature: 149

Protein sequence:

>149_residues
MNPIRMKKLESEIIRQISTAILEGKVKDPRVFLPSFHRIEISEDLKYAKVYFTALCNNNERKKLTQGLVSCAGFLSSFVG
KNLRLHTNPKFTFVWDNSYIKSLEVNRLIDDSAPKTLFEELHPNPEEDDGDTDAETLLEDSESGIERET

Sequences:

>Translated_149_residues
MNPIRMKKLESEIIRQISTAILEGKVKDPRVFLPSFHRIEISEDLKYAKVYFTALCNNNERKKLTQGLVSCAGFLSSFVG
KNLRLHTNPKFTFVWDNSYIKSLEVNRLIDDSAPKTLFEELHPNPEEDDGDTDAETLLEDSESGIERET
>Mature_149_residues
MNPIRMKKLESEIIRQISTAILEGKVKDPRVFLPSFHRIEISEDLKYAKVYFTALCNNNERKKLTQGLVSCAGFLSSFVG
KNLRLHTNPKFTFVWDNSYIKSLEVNRLIDDSAPKTLFEELHPNPEEDDGDTDAETLLEDSESGIERET

Specific function: Associates with free 30S ribosomal subunits (but not with 30S subunits that are part of 70S ribosomes or polysomes). Essential for efficient processing of 16S rRNA. May interact with the 5'-terminal helix region of 16S rRNA

COG id: COG0858

COG function: function code J; Ribosome-binding factor A

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rbfA family

Homologues:

Organism=Escherichia coli, GI1789558, Length=130, Percent_Identity=32.3076923076923, Blast_Score=71, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RBFA_LEPBA (B0SH19)

Other databases:

- EMBL:   CP000777
- RefSeq:   YP_001962564.1
- ProteinModelPortal:   B0SH19
- GeneID:   6388768
- GenomeReviews:   CP000777_GR
- KEGG:   lbf:LBF_1473
- HOGENOM:   HBG529270
- OMA:   INAKVFI
- ProtClustDB:   CLSK573715
- BioCyc:   LBIF355278:LBF_1473-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00003
- InterPro:   IPR015946
- InterPro:   IPR000238
- Gene3D:   G3DSA:3.30.300.20
- ProDom:   PD007327
- TIGRFAMs:   TIGR00082

Pfam domain/function: PF02033 RBFA; SSF89919 Rib_bd_factA

EC number: NA

Molecular weight: Translated: 17078; Mature: 17078

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS01319 RBFA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPIRMKKLESEIIRQISTAILEGKVKDPRVFLPSFHRIEISEDLKYAKVYFTALCNNNE
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCEEEECCCCHHHHHHHHHHHHCCCH
RKKLTQGLVSCAGFLSSFVGKNLRLHTNPKFTFVWDNSYIKSLEVNRLIDDSAPKTLFEE
HHHHHHHHHHHHHHHHHHHCCCCEEECCCCEEEEECCCHHHHHHHHHHHCCCCHHHHHHH
LHPNPEEDDGDTDAETLLEDSESGIERET
HCCCCCCCCCCCHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MNPIRMKKLESEIIRQISTAILEGKVKDPRVFLPSFHRIEISEDLKYAKVYFTALCNNNE
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCEEEECCCCHHHHHHHHHHHHCCCH
RKKLTQGLVSCAGFLSSFVGKNLRLHTNPKFTFVWDNSYIKSLEVNRLIDDSAPKTLFEE
HHHHHHHHHHHHHHHHHHHCCCCEEECCCCEEEEECCCHHHHHHHHHHHCCCCHHHHHHH
LHPNPEEDDGDTDAETLLEDSESGIERET
HCCCCCCCCCCCHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA