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The map label for this gene is rluB [H]

Identifier: 183220850

GI number: 183220850

Start: 1525402

End: 1526145

Strand: Direct

Name: rluB [H]

Synonym: LEPBI_I1463

Alternate gene names: 183220850

Gene position: 1525402-1526145 (Clockwise)

Preceding gene: 183220847

Following gene: 183220851

Centisome position: 42.38

GC content: 37.63

Gene sequence:

>744_bases
ATGAGGATCAATGCATTTCTTGCCAAATTAGGTTTAGGTTCCCGTAGAAAGGTCGAAGAATTGGTCCTCGCCGGTAGGGT
CAAAATCAACGGAAGTACCATCACAGACTTGTCTTTCCAGGTAAACGAAGAGGATTCTATTCTCTTTGATGGAAAACCTG
TGACAAAAGAAGAAGGTTCCGATTCTAGACCAAAAATCATCGCCTTTAATAAACCACCAGGGTTTCTCACATCCCATGAA
GACAAACACCATGAGAATACCATCTTTACATTATTACCAGAGTCGTTTCAAAAATACAATTATGCGGGACGCCTTGATTT
AGATTCGAGAGGGCTTATTCTATTATCCATCGATGGAAATTTCATTCAGAAGGTGACACATCCTAAAAATAAAATTGATA
AAGAATATATTATTAGTTTGAAACAACCTGTCAGTTGGAAAAAAATTGCCGAAGAATTTATGTTAGGTGTAAGAGAAGGT
GGAGAAACACTTAGAGCACTTGCTGTAAAACCAGCCAATGTATTCCCCGAAAAAACACCACCTGGTTTTACCAGTTACCT
CAGCATCGTCTTAAAAGAAGGGAAAAAAAGGCAAATCCGAAGGATGTGTAAAACAAAAGATTTGGTTGTTCTTGATTTGT
ATCGGATCCGCATTGGGAAATTAGATTTGCGAAATTTTGATCTATTGGAAGGAAAATACAAAGTGGTCACAGAAGAGCAA
GTTCTTGGAAATTTGAATCCTTAA

Upstream 100 bases:

>100_bases
AACGGTGGGGTTTAGATTTTTGGAGCGAGGTTTCTTTTTTAATGGATTCATACCTTCTTTAGACTTTTTGAAATGAGGCC
ACTTTGTAAAATGGAAAATC

Downstream 100 bases:

>100_bases
TTTAGTTGAAGAATCCACTCGACTCGTAAAATGGTGAGGTTAGGGGATTTGTTTTTTTGAAATCAAAACCATTTTTATTT
TACCCAGTTGTTCTCTTTTT

Product: putative ribosomal subunit pseudouridine synthase

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 247; Mature: 247

Protein sequence:

>247_residues
MRINAFLAKLGLGSRRKVEELVLAGRVKINGSTITDLSFQVNEEDSILFDGKPVTKEEGSDSRPKIIAFNKPPGFLTSHE
DKHHENTIFTLLPESFQKYNYAGRLDLDSRGLILLSIDGNFIQKVTHPKNKIDKEYIISLKQPVSWKKIAEEFMLGVREG
GETLRALAVKPANVFPEKTPPGFTSYLSIVLKEGKKRQIRRMCKTKDLVVLDLYRIRIGKLDLRNFDLLEGKYKVVTEEQ
VLGNLNP

Sequences:

>Translated_247_residues
MRINAFLAKLGLGSRRKVEELVLAGRVKINGSTITDLSFQVNEEDSILFDGKPVTKEEGSDSRPKIIAFNKPPGFLTSHE
DKHHENTIFTLLPESFQKYNYAGRLDLDSRGLILLSIDGNFIQKVTHPKNKIDKEYIISLKQPVSWKKIAEEFMLGVREG
GETLRALAVKPANVFPEKTPPGFTSYLSIVLKEGKKRQIRRMCKTKDLVVLDLYRIRIGKLDLRNFDLLEGKYKVVTEEQ
VLGNLNP
>Mature_247_residues
MRINAFLAKLGLGSRRKVEELVLAGRVKINGSTITDLSFQVNEEDSILFDGKPVTKEEGSDSRPKIIAFNKPPGFLTSHE
DKHHENTIFTLLPESFQKYNYAGRLDLDSRGLILLSIDGNFIQKVTHPKNKIDKEYIISLKQPVSWKKIAEEFMLGVREG
GETLRALAVKPANVFPEKTPPGFTSYLSIVLKEGKKRQIRRMCKTKDLVVLDLYRIRIGKLDLRNFDLLEGKYKVVTEEQ
VLGNLNP

Specific function: Responsible for synthesis of pseudouridine from uracil- 2605 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=242, Percent_Identity=31.404958677686, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI1787524, Length=227, Percent_Identity=26.8722466960352, Blast_Score=94, Evalue=8e-21,
Organism=Escherichia coli, GI1790453, Length=242, Percent_Identity=30.1652892561983, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI87081838, Length=221, Percent_Identity=29.8642533936652, Blast_Score=75, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 28040; Mature: 28040

Theoretical pI: Translated: 10.07; Mature: 10.07

Prosite motif: PS50889 S4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRINAFLAKLGLGSRRKVEELVLAGRVKINGSTITDLSFQVNEEDSILFDGKPVTKEEGS
CCHHHHHHHHCCCCCHHHHHHHHHCEEEECCCEEEEEEEEECCCCCEEECCCCCCCCCCC
DSRPKIIAFNKPPGFLTSHEDKHHENTIFTLLPESFQKYNYAGRLDLDSRGLILLSIDGN
CCCCEEEEECCCCCCCCCCCCCCCCCEEEEECCHHHHHCCCCCEEEECCCCEEEEEECCH
FIQKVTHPKNKIDKEYIISLKQPVSWKKIAEEFMLGVREGGETLRALAVKPANVFPEKTP
HHHHHCCCHHHCCHHHEEECCCCCCHHHHHHHHHHHHHCCCCEEEHEEECCCCCCCCCCC
PGFTSYLSIVLKEGKKRQIRRMCKTKDLVVLDLYRIRIGKLDLRNFDLLEGKYKVVTEEQ
CCHHHHHHHHHHCCHHHHHHHHHCCCCEEEEEEEEEEECEECCCCCEECCCCEEEEEHHH
VLGNLNP
HHCCCCC
>Mature Secondary Structure
MRINAFLAKLGLGSRRKVEELVLAGRVKINGSTITDLSFQVNEEDSILFDGKPVTKEEGS
CCHHHHHHHHCCCCCHHHHHHHHHCEEEECCCEEEEEEEEECCCCCEEECCCCCCCCCCC
DSRPKIIAFNKPPGFLTSHEDKHHENTIFTLLPESFQKYNYAGRLDLDSRGLILLSIDGN
CCCCEEEEECCCCCCCCCCCCCCCCCEEEEECCHHHHHCCCCCEEEECCCCEEEEEECCH
FIQKVTHPKNKIDKEYIISLKQPVSWKKIAEEFMLGVREGGETLRALAVKPANVFPEKTP
HHHHHCCCHHHCCHHHEEECCCCCCHHHHHHHHHHHHHCCCCEEEHEEECCCCCCCCCCC
PGFTSYLSIVLKEGKKRQIRRMCKTKDLVVLDLYRIRIGKLDLRNFDLLEGKYKVVTEEQ
CCHHHHHHHHHHCCHHHHHHHHHCCCCEEEEEEEEEEECEECCCCCEECCCCEEEEEHHH
VLGNLNP
HHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]