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The map label for this gene is ispD [H]

Identifier: 183220823

GI number: 183220823

Start: 1500315

End: 1501004

Strand: Direct

Name: ispD [H]

Synonym: LEPBI_I1435

Alternate gene names: 183220823

Gene position: 1500315-1501004 (Clockwise)

Preceding gene: 183220822

Following gene: 183220827

Centisome position: 41.68

GC content: 45.07

Gene sequence:

>690_bases
ATGAACCAACTCTTTGCCATCCTTCTCGCTGGTGGAACGGGTAGCCGAATGGGAGAATCTCTCCCAAAACAATTCTTACG
CTTAAGGGGGGAATCCCTCCTTAGGCATTCGGTGAAACGATTCCGCCGTTTTGGACTGCTTAAGTCACTGACAGTTGTTT
CCCATCCTGATTGGATCCTCGAAACAGAAAAAGAATTACAAGACTTACTGGAACCAAACGACCGTATTGTGGAAGGAGGG
GAAACAAGGCACCTTTCCACCTTGAAAGGACTTGCATCCATTTCCTATGATGAAAAGGATATTTTTTTCATCCATGATGT
GGCAAGACCAAGTTTCAAACAAAACGAACTCTTCCAACTTGTCGAACAAACCAAAATCTTTGGAGCCGCATCCATTGTTT
GTCCCGTGACTGAAAGTCTCATTCGCGTGGGGCGCCATCGAAACTATTCGGAAGAACCTTTGAATCGAGAAGAAGTGTAT
GCGGTAAAAACCCCGCAGTCTGTGGCAGGGTTTATGTTAAGCGAACTTTTGTTAGATCCACTTTCTTCAAAGACCTCAGA
ACATCCTACAGACCTTTGCACTTGGATGGGGAAACGAAAGGTTGGAATTGTCGAAACAGACTTTCGAAATACCAAAGTGA
CAAGTCCAGGCGATCTCACTCTAGCGGATTCCCTCTACATTGAGGACTAA

Upstream 100 bases:

>100_bases
AGACAGCTGAAGTTTTACTTCGAAAAAACGGAGAGACAAAACTCATTCGTAAAAAAGAACCAGTGATGGAAATCTTTCGC
AATGAAATCTTAGTGGTGGA

Downstream 100 bases:

>100_bases
ATCGAATTTACCGACCGTGGAAGATTTTAAACCTTTGTTTCCAAATCCAAACTCGAAATAAGTTTTGATTCTCAATCGAA
TCAATTTGGATTCCAAAACC

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT [H]

Number of amino acids: Translated: 229; Mature: 229

Protein sequence:

>229_residues
MNQLFAILLAGGTGSRMGESLPKQFLRLRGESLLRHSVKRFRRFGLLKSLTVVSHPDWILETEKELQDLLEPNDRIVEGG
ETRHLSTLKGLASISYDEKDIFFIHDVARPSFKQNELFQLVEQTKIFGAASIVCPVTESLIRVGRHRNYSEEPLNREEVY
AVKTPQSVAGFMLSELLLDPLSSKTSEHPTDLCTWMGKRKVGIVETDFRNTKVTSPGDLTLADSLYIED

Sequences:

>Translated_229_residues
MNQLFAILLAGGTGSRMGESLPKQFLRLRGESLLRHSVKRFRRFGLLKSLTVVSHPDWILETEKELQDLLEPNDRIVEGG
ETRHLSTLKGLASISYDEKDIFFIHDVARPSFKQNELFQLVEQTKIFGAASIVCPVTESLIRVGRHRNYSEEPLNREEVY
AVKTPQSVAGFMLSELLLDPLSSKTSEHPTDLCTWMGKRKVGIVETDFRNTKVTSPGDLTLADSLYIED
>Mature_229_residues
MNQLFAILLAGGTGSRMGESLPKQFLRLRGESLLRHSVKRFRRFGLLKSLTVVSHPDWILETEKELQDLLEPNDRIVEGG
ETRHLSTLKGLASISYDEKDIFFIHDVARPSFKQNELFQLVEQTKIFGAASIVCPVTESLIRVGRHRNYSEEPLNREEVY
AVKTPQSVAGFMLSELLLDPLSSKTSEHPTDLCTWMGKRKVGIVETDFRNTKVTSPGDLTLADSLYIED

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP) [H]

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family [H]

Homologues:

Organism=Homo sapiens, GI157412259, Length=143, Percent_Identity=27.972027972028, Blast_Score=69, Evalue=3e-12,
Organism=Escherichia coli, GI1789104, Length=229, Percent_Identity=27.5109170305677, Blast_Score=75, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001228 [H]

Pfam domain/function: PF01128 IspD [H]

EC number: =2.7.7.60 [H]

Molecular weight: Translated: 25891; Mature: 25891

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQLFAILLAGGTGSRMGESLPKQFLRLRGESLLRHSVKRFRRFGLLKSLTVVSHPDWIL
CCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
ETEKELQDLLEPNDRIVEGGETRHLSTLKGLASISYDEKDIFFIHDVARPSFKQNELFQL
CCHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHH
VEQTKIFGAASIVCPVTESLIRVGRHRNYSEEPLNREEVYAVKTPQSVAGFMLSELLLDP
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHEEEEECCHHHHHHHHHHHHHHH
LSSKTSEHPTDLCTWMGKRKVGIVETDFRNTKVTSPGDLTLADSLYIED
HHCCCCCCHHHHHHHHCCCCCCEEEECCCCCCCCCCCCEEEECCEEECC
>Mature Secondary Structure
MNQLFAILLAGGTGSRMGESLPKQFLRLRGESLLRHSVKRFRRFGLLKSLTVVSHPDWIL
CCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
ETEKELQDLLEPNDRIVEGGETRHLSTLKGLASISYDEKDIFFIHDVARPSFKQNELFQL
CCHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHH
VEQTKIFGAASIVCPVTESLIRVGRHRNYSEEPLNREEVYAVKTPQSVAGFMLSELLLDP
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHEEEEECCHHHHHHHHHHHHHHH
LSSKTSEHPTDLCTWMGKRKVGIVETDFRNTKVTSPGDLTLADSLYIED
HHCCCCCCHHHHHHHHCCCCCCEEEECCCCCCCCCCCCEEEECCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA