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The map label for this gene is nth [H]

Identifier: 183220424

GI number: 183220424

Start: 1064891

End: 1065532

Strand: Direct

Name: nth [H]

Synonym: LEPBI_I1021

Alternate gene names: 183220424

Gene position: 1064891-1065532 (Clockwise)

Preceding gene: 183220423

Following gene: 183220426

Centisome position: 29.58

GC content: 46.11

Gene sequence:

>642_bases
TTGAAACGATCCAAAAAAACACCAAAAATCACCGAAGTCTACCGTCTCTTGGAGGCGGAGTTCGGTGTCGTAGAAACCCC
CCTTACATTTTCTATACCATACGAGCTTGCCATCGCTGTCATCCTCAGTGCACAGTGTACGGACGAACGTGTGAACCAAG
TCACACCTGAACTCTTTCTTGCATTTCCTACCCTCGAGTCCTTTGCGAAAGCCCCTCTTTCTGCGATTGAAACGAAAATT
TTCTCCACTGGGTTTTATAAAAACAAAGCCAAAAGCATCCAAGGGTTTGCGAGGATGGTTCTTTCTGAATTTGGTGGGGA
ACTTCCAAAGACAATGGAGGAGGCGATTCGGCTCCCTGGGTTCGGGAGAAAAACCGCCAATGTGGTGCTTGCTGAAATTT
ATGGAGTGGTGGAAGGATTTGTGGTGGATACCCATGTGAAACGGCTCACAAAACGCCTCGGGTTTACCAAAAAAACGGAT
CCCATTCAAATCGAACGGGAAATGATGAAGATCACTCCTAAGGAAATTTGCCGAAACCTATCTCTTTACCTAATATTTCT
CGGTCGGAAGTATTGCCAGGCTCGCCGTACATTTTGTTCGGATTGCCCTCTTTCTTCCCTATGTCCTTCTTATTCGGAAT
AG

Upstream 100 bases:

>100_bases
GTGACATCACTGCGATCACTCCTAAAAAATACGTAGGAATCACTCCCAAAGCACAACCAGTAGCAACTGCTTAAATTTCA
GATTTAAGTTTGTTTGTGGA

Downstream 100 bases:

>100_bases
GTTGTTCCAAACCTTCTTCTGTTTCTGATTCCTTTCTTTGTTTCCAAGAACGATTGCGGTCCGTGAGATTGATGGATTCC
AGTTTATAATCCATTCGAAT

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MKRSKKTPKITEVYRLLEAEFGVVETPLTFSIPYELAIAVILSAQCTDERVNQVTPELFLAFPTLESFAKAPLSAIETKI
FSTGFYKNKAKSIQGFARMVLSEFGGELPKTMEEAIRLPGFGRKTANVVLAEIYGVVEGFVVDTHVKRLTKRLGFTKKTD
PIQIEREMMKITPKEICRNLSLYLIFLGRKYCQARRTFCSDCPLSSLCPSYSE

Sequences:

>Translated_213_residues
MKRSKKTPKITEVYRLLEAEFGVVETPLTFSIPYELAIAVILSAQCTDERVNQVTPELFLAFPTLESFAKAPLSAIETKI
FSTGFYKNKAKSIQGFARMVLSEFGGELPKTMEEAIRLPGFGRKTANVVLAEIYGVVEGFVVDTHVKRLTKRLGFTKKTD
PIQIEREMMKITPKEICRNLSLYLIFLGRKYCQARRTFCSDCPLSSLCPSYSE
>Mature_213_residues
MKRSKKTPKITEVYRLLEAEFGVVETPLTFSIPYELAIAVILSAQCTDERVNQVTPELFLAFPTLESFAKAPLSAIETKI
FSTGFYKNKAKSIQGFARMVLSEFGGELPKTMEEAIRLPGFGRKTANVVLAEIYGVVEGFVVDTHVKRLTKRLGFTKKTD
PIQIEREMMKITPKEICRNLSLYLIFLGRKYCQARRTFCSDCPLSSLCPSYSE

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=179, Percent_Identity=27.9329608938547, Blast_Score=99, Evalue=2e-21,
Organism=Escherichia coli, GI1787920, Length=183, Percent_Identity=37.7049180327869, Blast_Score=124, Evalue=6e-30,
Organism=Escherichia coli, GI1789331, Length=221, Percent_Identity=27.6018099547511, Blast_Score=71, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17554540, Length=179, Percent_Identity=29.608938547486, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6324530, Length=194, Percent_Identity=26.2886597938144, Blast_Score=77, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6319304, Length=194, Percent_Identity=27.319587628866, Blast_Score=73, Evalue=4e-14,
Organism=Drosophila melanogaster, GI45550361, Length=179, Percent_Identity=26.2569832402235, Blast_Score=89, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 24056; Mature: 24056

Theoretical pI: Translated: 9.39; Mature: 9.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRSKKTPKITEVYRLLEAEFGVVETPLTFSIPYELAIAVILSAQCTDERVNQVTPELFL
CCCCCCCCHHHHHHHHHHHHCCCCCCCEEEECHHHHHHHHHHHHHCHHHHHHHHCHHHHH
AFPTLESFAKAPLSAIETKIFSTGFYKNKAKSIQGFARMVLSEFGGELPKTMEEAIRLPG
HHHHHHHHHHCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCC
FGRKTANVVLAEIYGVVEGFVVDTHVKRLTKRLGFTKKTDPIQIEREMMKITPKEICRNL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCHHHHHHHH
SLYLIFLGRKYCQARRTFCSDCPLSSLCPSYSE
HHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCC
>Mature Secondary Structure
MKRSKKTPKITEVYRLLEAEFGVVETPLTFSIPYELAIAVILSAQCTDERVNQVTPELFL
CCCCCCCCHHHHHHHHHHHHCCCCCCCEEEECHHHHHHHHHHHHHCHHHHHHHHCHHHHH
AFPTLESFAKAPLSAIETKIFSTGFYKNKAKSIQGFARMVLSEFGGELPKTMEEAIRLPG
HHHHHHHHHHCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCC
FGRKTANVVLAEIYGVVEGFVVDTHVKRLTKRLGFTKKTDPIQIEREMMKITPKEICRNL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCHHHHHHHH
SLYLIFLGRKYCQARRTFCSDCPLSSLCPSYSE
HHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]