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The map label for this gene is tilS [H]

Identifier: 183219981

GI number: 183219981

Start: 561933

End: 562787

Strand: Reverse

Name: tilS [H]

Synonym: LEPBI_I0563

Alternate gene names: 183219981

Gene position: 562787-561933 (Counterclockwise)

Preceding gene: 183219982

Following gene: 183219980

Centisome position: 15.63

GC content: 33.57

Gene sequence:

>855_bases
TTGCATCTAACGAGAGGTGGTGGTAAAAAATCTTTTTATACTCTCCCTCCATTTGATGGAGAAAGGTTTTTGCCTCTTGT
TTTTTTGGAAGACCATGAATTGAACGAATTATACCAGTTTGTATCAGCAAATATGCGTATCTTTGAAGATGAATCAAATG
GTGATCCTATTTATAAAAGAAATAGAATTCGGATGGAATTGATTCCAATTTTAGAAAGAGAAAAATGGAACTTTTTTAAA
ACCTATTGGAATTTCCACGATCGATCGCAACTCAATTTACAATTTGATGGTTTTTCAAACCAATCCCAAAGTTATGCACC
ACACCTATTTCGAATTCCTCATGATACATGGGTGAGTTTGAATTTATCTGCTAAAAAAGAATTAATCGATTTCCATTTGA
AACTGATGGGTATGTATCCCTTATATAAATCTGGCTTTGAGAACTTTCACCTGCAATCAGAAGGGGAAAGAGCATTTTTA
GAAAATAAAAATTGTTACCTATACAAATCAAAGTTTGGTGATCTCTTCATCATTGACAAAAAATCTCCAGCATTTAAAAA
AGCGATTTCCAATCGAGAGGGCCAAATTTTGGTCATTGAGTGGAACCAAAATCGATTCAAAATCAATGATCCTGATATGA
AATACAGTTTAGGATCCTGGCACCATGGGCAAAAAATCCAAATTCGTTCTGGAAATAAGGAAATTTCCGAATGTATGCGG
GAAAATGGTGTTCCGTTTTTTTTACGATCCTATATCCCTATCCTCTATTTTGAGAATGAACCGATCCAAATTTTATTTTC
ACTCTTTTCAAAAAACGAAAAGAATTATCCGAAACGAATCTATTTAGAAAGGTGA

Upstream 100 bases:

>100_bases
ACGGCTTGTTCGTTACCATGAATTAAAAAAAATAAGCAAAAAAATTCCTTCCATCATTCTCACTGGCCATCACTGCAAAG
ATTATACAGAATCCATTTTT

Downstream 100 bases:

>100_bases
TCTAGATGCATAAATATTCCAAAGAAATACCCTTTCCTGAAACTAAATTCTTCACTTCTATCGCCAAATTAGATGAAAAA
GAAGATTTGGATTCAGTACA

Product: hypothetical protein

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MHLTRGGGKKSFYTLPPFDGERFLPLVFLEDHELNELYQFVSANMRIFEDESNGDPIYKRNRIRMELIPILEREKWNFFK
TYWNFHDRSQLNLQFDGFSNQSQSYAPHLFRIPHDTWVSLNLSAKKELIDFHLKLMGMYPLYKSGFENFHLQSEGERAFL
ENKNCYLYKSKFGDLFIIDKKSPAFKKAISNREGQILVIEWNQNRFKINDPDMKYSLGSWHHGQKIQIRSGNKEISECMR
ENGVPFFLRSYIPILYFENEPIQILFSLFSKNEKNYPKRIYLER

Sequences:

>Translated_284_residues
MHLTRGGGKKSFYTLPPFDGERFLPLVFLEDHELNELYQFVSANMRIFEDESNGDPIYKRNRIRMELIPILEREKWNFFK
TYWNFHDRSQLNLQFDGFSNQSQSYAPHLFRIPHDTWVSLNLSAKKELIDFHLKLMGMYPLYKSGFENFHLQSEGERAFL
ENKNCYLYKSKFGDLFIIDKKSPAFKKAISNREGQILVIEWNQNRFKINDPDMKYSLGSWHHGQKIQIRSGNKEISECMR
ENGVPFFLRSYIPILYFENEPIQILFSLFSKNEKNYPKRIYLER
>Mature_284_residues
MHLTRGGGKKSFYTLPPFDGERFLPLVFLEDHELNELYQFVSANMRIFEDESNGDPIYKRNRIRMELIPILEREKWNFFK
TYWNFHDRSQLNLQFDGFSNQSQSYAPHLFRIPHDTWVSLNLSAKKELIDFHLKLMGMYPLYKSGFENFHLQSEGERAFL
ENKNCYLYKSKFGDLFIIDKKSPAFKKAISNREGQILVIEWNQNRFKINDPDMKYSLGSWHHGQKIQIRSGNKEISECMR
ENGVPFFLRSYIPILYFENEPIQILFSLFSKNEKNYPKRIYLER

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012795
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3 [H]

EC number: NA

Molecular weight: Translated: 34060; Mature: 34060

Theoretical pI: Translated: 9.27; Mature: 9.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHLTRGGGKKSFYTLPPFDGERFLPLVFLEDHELNELYQFVSANMRIFEDESNGDPIYKR
CCCCCCCCCCCEEECCCCCCCCEEEEEEECCCCHHHHHHHHHCCEEEEECCCCCCCCEEC
NRIRMELIPILEREKWNFFKTYWNFHDRSQLNLQFDGFSNQSQSYAPHLFRIPHDTWVSL
CCEEEEEEEEECCCCCCEEEEEECCCCCCEEEEEECCCCCCCCCCCCEEEECCCCCEEEE
NLSAKKELIDFHLKLMGMYPLYKSGFENFHLQSEGERAFLENKNCYLYKSKFGDLFIIDK
ECHHHHHHHHHHHHHHCCCHHHHCCCCCEEECCCCCEEEECCCCEEEEECCCCCEEEEEC
KSPAFKKAISNREGQILVIEWNQNRFKINDPDMKYSLGSWHHGQKIQIRSGNKEISECMR
CCHHHHHHHCCCCCEEEEEEECCCEEEECCCCCEEECCCCCCCCEEEEECCCHHHHHHHH
ENGVPFFLRSYIPILYFENEPIQILFSLFSKNEKNYPKRIYLER
HCCCCEEECCCCEEEEECCCHHHHHHHHHHCCCCCCCCEEEEEC
>Mature Secondary Structure
MHLTRGGGKKSFYTLPPFDGERFLPLVFLEDHELNELYQFVSANMRIFEDESNGDPIYKR
CCCCCCCCCCCEEECCCCCCCCEEEEEEECCCCHHHHHHHHHCCEEEEECCCCCCCCEEC
NRIRMELIPILEREKWNFFKTYWNFHDRSQLNLQFDGFSNQSQSYAPHLFRIPHDTWVSL
CCEEEEEEEEECCCCCCEEEEEECCCCCCEEEEEECCCCCCCCCCCCEEEECCCCCEEEE
NLSAKKELIDFHLKLMGMYPLYKSGFENFHLQSEGERAFLENKNCYLYKSKFGDLFIIDK
ECHHHHHHHHHHHHHHCCCHHHHCCCCCEEECCCCCEEEECCCCEEEEECCCCCEEEEEC
KSPAFKKAISNREGQILVIEWNQNRFKINDPDMKYSLGSWHHGQKIQIRSGNKEISECMR
CCHHHHHHHCCCCCEEEEEEECCCEEEECCCCCEEECCCCCCCCEEEEECCCHHHHHHHH
ENGVPFFLRSYIPILYFENEPIQILFSLFSKNEKNYPKRIYLER
HCCCCEEECCCCEEEEECCCHHHHHHHHHHCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA