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The map label for this gene is kdsB

Identifier: 183219624

GI number: 183219624

Start: 191855

End: 192595

Strand: Direct

Name: kdsB

Synonym: LEPBI_I0199

Alternate gene names: 183219624

Gene position: 191855-192595 (Clockwise)

Preceding gene: 183219623

Following gene: 183219630

Centisome position: 5.33

GC content: 45.75

Gene sequence:

>741_bases
ATGTCCGACCAAATTCTCGGTGTGATCCCTGCACGCTTTGCGAGCACACGATTTCCTGGAAAACCACTGGCCCTCATTGG
AACCAAACCAATGATCCAGTGGACCTACCACCACGCATCCCTTTCAAAGTCTTTCCACCGTTTGGTGGTCGCCACCGATG
ACAAACGAATCCATGATGTGGTATTGGGTTTTGGGGGTGAGTCTGTTCTCACAAGCCCCGATCATCCCACTGGTACCGAC
AGAATCATTGAAGTAGCAGAAACCTATCCCAATTACGGAATCATCGTGAATATCCAAGGGGATGAACCCGGAATGGAAGC
TTCCTTAATTGATGGAGTGGTGGGTTTAAAAACCAAACACCGAAATTGGGAAATGACAACTGCCGCCGTGCCATTTACAA
GTGCAGAAGATCCAAAAGACCCTAACAAAGTTAAGGTGGTTTTTGACAATAAAGGACGAGCCAATTATTTTTCTCGTTCA
CCGATCCCGGCCTCCTTTAAGGGAGAAGCGAAGTACCATCGTCATTTAGGCATTTATGCTTACGAACGTGACTTCTTAAT
GAATTATAACCAACTCCCTCCATCCGATTGGGAAACGGTAGAGTCTCTGGAACAACTGCGTGCCTTACAAAATGGATCAA
CCATTGGGGTGTATCTTTCTGACAAAGCCAATCTTGGTGTGGATTCGCCGGCTGACTTAGAAGTGGTGATTACAGAATTT
AAAAAGAAAGGTTTACTTTAG

Upstream 100 bases:

>100_bases
TGGATTTACGTGAGGAAATCAAAGCCCACTTAAATCACATTTTGACGAATGGAAAAATCAAAGAGGTTTACTTTACCGAG
TTCTTAGTAAACTAGGACCT

Downstream 100 bases:

>100_bases
ATAGCAAATTCCATTCGATCATTGGATCGGAAATAAGTAGCAAAAAAATTAAGCTTTGATTTCTATCTCAATCGTTTCCC
TTTTCCTTTACTTTTCCACG

Product: 3-deoxy-manno-octulosonate cytidylyltransferase

Products: NA

Alternate protein names: CMP-2-keto-3-deoxyoctulosonic acid synthase; CKS; CMP-KDO synthase

Number of amino acids: Translated: 246; Mature: 245

Protein sequence:

>246_residues
MSDQILGVIPARFASTRFPGKPLALIGTKPMIQWTYHHASLSKSFHRLVVATDDKRIHDVVLGFGGESVLTSPDHPTGTD
RIIEVAETYPNYGIIVNIQGDEPGMEASLIDGVVGLKTKHRNWEMTTAAVPFTSAEDPKDPNKVKVVFDNKGRANYFSRS
PIPASFKGEAKYHRHLGIYAYERDFLMNYNQLPPSDWETVESLEQLRALQNGSTIGVYLSDKANLGVDSPADLEVVITEF
KKKGLL

Sequences:

>Translated_246_residues
MSDQILGVIPARFASTRFPGKPLALIGTKPMIQWTYHHASLSKSFHRLVVATDDKRIHDVVLGFGGESVLTSPDHPTGTD
RIIEVAETYPNYGIIVNIQGDEPGMEASLIDGVVGLKTKHRNWEMTTAAVPFTSAEDPKDPNKVKVVFDNKGRANYFSRS
PIPASFKGEAKYHRHLGIYAYERDFLMNYNQLPPSDWETVESLEQLRALQNGSTIGVYLSDKANLGVDSPADLEVVITEF
KKKGLL
>Mature_245_residues
SDQILGVIPARFASTRFPGKPLALIGTKPMIQWTYHHASLSKSFHRLVVATDDKRIHDVVLGFGGESVLTSPDHPTGTDR
IIEVAETYPNYGIIVNIQGDEPGMEASLIDGVVGLKTKHRNWEMTTAAVPFTSAEDPKDPNKVKVVFDNKGRANYFSRSP
IPASFKGEAKYHRHLGIYAYERDFLMNYNQLPPSDWETVESLEQLRALQNGSTIGVYLSDKANLGVDSPADLEVVITEFK
KKGLL

Specific function: Activates KDO (a required 8-carbon sugar) for incorporation into bacterial lipopolysaccharide in Gram-negative bacteria

COG id: COG1212

COG function: function code M; CMP-2-keto-3-deoxyoctulosonic acid synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the kdsB family

Homologues:

Organism=Escherichia coli, GI1787147, Length=245, Percent_Identity=42.4489795918367, Blast_Score=167, Evalue=6e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KDSB_LEPBA (B0SA58)

Other databases:

- EMBL:   CP000777
- RefSeq:   YP_001961318.1
- ProteinModelPortal:   B0SA58
- SMR:   B0SA58
- GeneID:   6388152
- GenomeReviews:   CP000777_GR
- KEGG:   lbf:LBF_0194
- HOGENOM:   HBG637773
- OMA:   IIPARLK
- ProtClustDB:   PRK05450
- BioCyc:   LBIF355278:LBF_0194-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00057
- InterPro:   IPR003329
- InterPro:   IPR004528
- TIGRFAMs:   TIGR00466

Pfam domain/function: PF02348 CTP_transf_3

EC number: =2.7.7.38

Molecular weight: Translated: 27285; Mature: 27154

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDQILGVIPARFASTRFPGKPLALIGTKPMIQWTYHHASLSKSFHRLVVATDDKRIHDV
CCCCEEEEECHHHHCCCCCCCCEEEEECCCEEEEEEEHHHHHCCCEEEEEEECCCEEEEE
VLGFGGESVLTSPDHPTGTDRIIEVAETYPNYGIIVNIQGDEPGMEASLIDGVVGLKTKH
EEECCCCCEECCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHCCEECC
RNWEMTTAAVPFTSAEDPKDPNKVKVVFDNKGRANYFSRSPIPASFKGEAKYHRHLGIYA
CCEEEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEHEEEEEE
YERDFLMNYNQLPPSDWETVESLEQLRALQNGSTIGVYLSDKANLGVDSPADLEVVITEF
EECHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHHHEEHH
KKKGLL
HHCCCC
>Mature Secondary Structure 
SDQILGVIPARFASTRFPGKPLALIGTKPMIQWTYHHASLSKSFHRLVVATDDKRIHDV
CCCEEEEECHHHHCCCCCCCCEEEEECCCEEEEEEEHHHHHCCCEEEEEEECCCEEEEE
VLGFGGESVLTSPDHPTGTDRIIEVAETYPNYGIIVNIQGDEPGMEASLIDGVVGLKTKH
EEECCCCCEECCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHCCEECC
RNWEMTTAAVPFTSAEDPKDPNKVKVVFDNKGRANYFSRSPIPASFKGEAKYHRHLGIYA
CCEEEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEHEEEEEE
YERDFLMNYNQLPPSDWETVESLEQLRALQNGSTIGVYLSDKANLGVDSPADLEVVITEF
EECHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHHHEEHH
KKKGLL
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA