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The map label for this gene is maeB [H]

Identifier: 183219568

GI number: 183219568

Start: 139288

End: 140589

Strand: Direct

Name: maeB [H]

Synonym: LEPBI_I0143

Alternate gene names: 183219568

Gene position: 139288-140589 (Clockwise)

Preceding gene: 183219564

Following gene: 183219569

Centisome position: 3.87

GC content: 43.78

Gene sequence:

>1302_bases
ATGAAAAATCACGCGCTTGAGTATCACTCTAGGTTTCCGAAAGGAAAAACCAAAGTAGTTCCGACAAAACCAACGGAGAA
CAGTTATGACCTGTCTTTGGCATACTCACCTGGCGTCGCATACCCTTGTCTTGAGATTGAAAAACAACCTGATCTCGTTT
ATGAATATACCAACCGAGGGAATTTAGTTGGTATCATCACCAATGGAACTGCCATTTTAGGTCTTGGCAACATTGGAGCT
TCCGCTGGAAAACCAGTGATGGAAGGAAAAGCTGTCTTATTTAAAAAATTTGCAGGAATCGATGTATTTGATATTGAAAT
CAACGAAACAGATCCTGAAAAATTCATCACGATTGTAAAGGCCCTTGAACCAACGTTTGGTGGGATCAATTTAGAAGACA
TCCGTGCCCCGGAATGCTTTCATATCGAAAAAACATTAGACGAGAACATGAAAATCCCTGTTTTCCATGATGACCAACAT
GGAACTGCGATCATCTCCACTGCGGCACTTCTCAATTCACTTACAATCACTGGGAAAAAAGCAGAAAACTTGAAGGTTGT
GATCAATGGAGCAGGGGCTGCTGCGATTTCGATCGCTCAGATGTTGACTCATATCGGAGTGAAACACGAATCCATTTTTA
TGTTGGATTCTCGTGGTGTCATCAATCACAAACGTACGAATTTACATGAATCCAAACTTCCATTTGTTCGAAACACAGAT
GCCGAAACTTTAGCAGATATCTTTCCTGGAACAGACCTCTTTATTGGTGTGTCGGTTGCGAATGTAGTAACAGAAGCAAT
GGTGAAAACCATGGCTGAGAAACCGATTATGTTTGCCCTTGCCAATCCTGATCCAGAGATTCCTTATCCTGATGCCAAAC
GGGCAAGACCAGATCTCATCATGGCAACAGGTCGCAGTGATTATCCTAACCAAGTGAATAACGTACTTGGGTTTCCCTTT
ATCTTTCGCGGGGCCCTTGATGTCCGTGCCAAAGTGGTGAACATGGAAATGAAACTCGCAGCGGCATACGCTCTCAGTGA
ACTCACAAAAATCCCTGTTCCGATCGAAGTATGCCAAGCGTATAACGAAGTGGAAATTCGATTTGGGGAAGACTATATCA
TTCCGAAACCACTCGACGAACGTGTGTTATACCATGTTGCGCCAGCAGTTGCGGAAGCGGCTGTGAAAACAGGTGTGAAC
CAAGTGGCCTACCCTGGTCGCGAGGCTTATGTGAAGTTTTTGGAATCGATCATGGCACAACAAAAAGAGCCAATCAGCGC
TCTAGAAATCGAAACCGATTGA

Upstream 100 bases:

>100_bases
CTGAAGGGACATAGAACATAGAGCAACCTTTTTTGGAGGTGCTTTCTCTCAATTTATTCTAGGATTTTCCATTGAATCAT
AAATCATGGGAAAATAGACC

Downstream 100 bases:

>100_bases
TACAGGGCCGAATTTCCTAGAGATAGACAAAAAAATTTCAAAAAAACTGACCCATCCTAGAAAAAGTACTTGTTTAAATT
TGTGCAACGCACATAACAGT

Product: NADP-dependent malic enzyme

Products: NA

Alternate protein names: NADP-ME [H]

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MKNHALEYHSRFPKGKTKVVPTKPTENSYDLSLAYSPGVAYPCLEIEKQPDLVYEYTNRGNLVGIITNGTAILGLGNIGA
SAGKPVMEGKAVLFKKFAGIDVFDIEINETDPEKFITIVKALEPTFGGINLEDIRAPECFHIEKTLDENMKIPVFHDDQH
GTAIISTAALLNSLTITGKKAENLKVVINGAGAAAISIAQMLTHIGVKHESIFMLDSRGVINHKRTNLHESKLPFVRNTD
AETLADIFPGTDLFIGVSVANVVTEAMVKTMAEKPIMFALANPDPEIPYPDAKRARPDLIMATGRSDYPNQVNNVLGFPF
IFRGALDVRAKVVNMEMKLAAAYALSELTKIPVPIEVCQAYNEVEIRFGEDYIIPKPLDERVLYHVAPAVAEAAVKTGVN
QVAYPGREAYVKFLESIMAQQKEPISALEIETD

Sequences:

>Translated_433_residues
MKNHALEYHSRFPKGKTKVVPTKPTENSYDLSLAYSPGVAYPCLEIEKQPDLVYEYTNRGNLVGIITNGTAILGLGNIGA
SAGKPVMEGKAVLFKKFAGIDVFDIEINETDPEKFITIVKALEPTFGGINLEDIRAPECFHIEKTLDENMKIPVFHDDQH
GTAIISTAALLNSLTITGKKAENLKVVINGAGAAAISIAQMLTHIGVKHESIFMLDSRGVINHKRTNLHESKLPFVRNTD
AETLADIFPGTDLFIGVSVANVVTEAMVKTMAEKPIMFALANPDPEIPYPDAKRARPDLIMATGRSDYPNQVNNVLGFPF
IFRGALDVRAKVVNMEMKLAAAYALSELTKIPVPIEVCQAYNEVEIRFGEDYIIPKPLDERVLYHVAPAVAEAAVKTGVN
QVAYPGREAYVKFLESIMAQQKEPISALEIETD
>Mature_433_residues
MKNHALEYHSRFPKGKTKVVPTKPTENSYDLSLAYSPGVAYPCLEIEKQPDLVYEYTNRGNLVGIITNGTAILGLGNIGA
SAGKPVMEGKAVLFKKFAGIDVFDIEINETDPEKFITIVKALEPTFGGINLEDIRAPECFHIEKTLDENMKIPVFHDDQH
GTAIISTAALLNSLTITGKKAENLKVVINGAGAAAISIAQMLTHIGVKHESIFMLDSRGVINHKRTNLHESKLPFVRNTD
AETLADIFPGTDLFIGVSVANVVTEAMVKTMAEKPIMFALANPDPEIPYPDAKRARPDLIMATGRSDYPNQVNNVLGFPF
IFRGALDVRAKVVNMEMKLAAAYALSELTKIPVPIEVCQAYNEVEIRFGEDYIIPKPLDERVLYHVAPAVAEAAVKTGVN
QVAYPGREAYVKFLESIMAQQKEPISALEIETD

Specific function: Unknown

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the phosphate acetyltransferase and butyryltransferase family [H]

Homologues:

Organism=Homo sapiens, GI62420882, Length=324, Percent_Identity=27.4691358024691, Blast_Score=83, Evalue=6e-16,
Organism=Homo sapiens, GI62420880, Length=324, Percent_Identity=27.4691358024691, Blast_Score=83, Evalue=6e-16,
Organism=Homo sapiens, GI239049447, Length=324, Percent_Identity=27.4691358024691, Blast_Score=83, Evalue=6e-16,
Organism=Homo sapiens, GI4505143, Length=406, Percent_Identity=25.1231527093596, Blast_Score=79, Evalue=8e-15,
Organism=Escherichia coli, GI1788806, Length=401, Percent_Identity=59.1022443890274, Blast_Score=474, Evalue=1e-135,
Organism=Escherichia coli, GI87081919, Length=450, Percent_Identity=24.8888888888889, Blast_Score=69, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17537199, Length=352, Percent_Identity=27.5568181818182, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6322823, Length=271, Percent_Identity=28.7822878228782, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI281362674, Length=419, Percent_Identity=28.4009546539379, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI281362672, Length=419, Percent_Identity=28.4009546539379, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI21356279, Length=419, Percent_Identity=28.4009546539379, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI78707242, Length=393, Percent_Identity=26.4631043256997, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI78707238, Length=393, Percent_Identity=26.4631043256997, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI78707240, Length=391, Percent_Identity=26.3427109974425, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI281363503, Length=366, Percent_Identity=24.3169398907104, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI281363505, Length=366, Percent_Identity=24.3169398907104, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI78707236, Length=366, Percent_Identity=24.3169398907104, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI78707232, Length=366, Percent_Identity=24.5901639344262, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR012188
- InterPro:   IPR016040
- InterPro:   IPR002505 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M; PF01515 PTA_PTB [H]

EC number: =1.1.1.40 [H]

Molecular weight: Translated: 47465; Mature: 47465

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: PS00331 MALIC_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNHALEYHSRFPKGKTKVVPTKPTENSYDLSLAYSPGVAYPCLEIEKQPDLVYEYTNRG
CCCCCCHHHHHCCCCCEEEECCCCCCCCEEEEEEECCCCCCCEEEECCCCCEEEEECCCC
NLVGIITNGTAILGLGNIGASAGKPVMEGKAVLFKKFAGIDVFDIEINETDPEKFITIVK
CEEEEEECCEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCEEEEEEECCCCHHHHHHHHH
ALEPTFGGINLEDIRAPECFHIEKTLDENMKIPVFHDDQHGTAIISTAALLNSLTITGKK
HHCCCCCCCCHHHCCCCCEEEEHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHEEEECCC
AENLKVVINGAGAAAISIAQMLTHIGVKHESIFMLDSRGVINHKRTNLHESKLPFVRNTD
CCCEEEEEECCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCHHCCCEEECCC
AETLADIFPGTDLFIGVSVANVVTEAMVKTMAEKPIMFALANPDPEIPYPDAKRARPDLI
HHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCEE
MATGRSDYPNQVNNVLGFPFIFRGALDVRAKVVNMEMKLAAAYALSELTKIPVPIEVCQA
EECCCCCCCHHHHHHCCCHHHHHCHHHHEEEEEEHHHHHHHHHHHHHHHCCCCCHHHHCC
YNEVEIRFGEDYIIPKPLDERVLYHVAPAVAEAAVKTGVNQVAYPGREAYVKFLESIMAQ
CCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHH
QKEPISALEIETD
CCCCCEEEEEECC
>Mature Secondary Structure
MKNHALEYHSRFPKGKTKVVPTKPTENSYDLSLAYSPGVAYPCLEIEKQPDLVYEYTNRG
CCCCCCHHHHHCCCCCEEEECCCCCCCCEEEEEEECCCCCCCEEEECCCCCEEEEECCCC
NLVGIITNGTAILGLGNIGASAGKPVMEGKAVLFKKFAGIDVFDIEINETDPEKFITIVK
CEEEEEECCEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCEEEEEEECCCCHHHHHHHHH
ALEPTFGGINLEDIRAPECFHIEKTLDENMKIPVFHDDQHGTAIISTAALLNSLTITGKK
HHCCCCCCCCHHHCCCCCEEEEHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHEEEECCC
AENLKVVINGAGAAAISIAQMLTHIGVKHESIFMLDSRGVINHKRTNLHESKLPFVRNTD
CCCEEEEEECCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCHHCCCEEECCC
AETLADIFPGTDLFIGVSVANVVTEAMVKTMAEKPIMFALANPDPEIPYPDAKRARPDLI
HHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCEE
MATGRSDYPNQVNNVLGFPFIFRGALDVRAKVVNMEMKLAAAYALSELTKIPVPIEVCQA
EECCCCCCCHHHHHHCCCHHHHHCHHHHEEEEEEHHHHHHHHHHHHHHHCCCCCHHHHCC
YNEVEIRFGEDYIIPKPLDERVLYHVAPAVAEAAVKTGVNQVAYPGREAYVKFLESIMAQ
CCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHH
QKEPISALEIETD
CCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 36376 [H]