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The map label for this gene is kinA [H]

Identifier: 183219504

GI number: 183219504

Start: 77254

End: 78765

Strand: Reverse

Name: kinA [H]

Synonym: LEPBI_I0077

Alternate gene names: 183219504

Gene position: 78765-77254 (Counterclockwise)

Preceding gene: 183219505

Following gene: 183219503

Centisome position: 2.19

GC content: 36.71

Gene sequence:

>1512_bases
ATGGATAAAATTTTAATCATCGATGATGAAGAAGACATTCGTATCGCTTTGAAACGTGTATTATCACGTGAAGGTTACCA
AATCGAACTTTCGGAATCTGCTTCGGATGCTGTGAAAAGAATTGAGACGGGCGAAACGTTTTCCCTAGTCATATCTGATA
TATTAATGTCCGGCATGTCTGGGATTGATTTTACCAAATTCATTGCGGAAAAAAATATCAACCTACCTGTGATCTTGATT
ACAGGAAATCCAAATCTATCTTCAGCGGAAGCTGCCATCCGATATCATGCATATGAATATATTTCCAAACCTGTAGACAG
GACACAATTACTCTCTGTCGTAAAACGAGCATTAGAAGTCAAAAATCAAAAAGATTCTGACTTAGAAAAATTATTGTTAT
CCGAAAAACTTGAGAAGGCACTCCGTACTCAAAACTTAGACTTAAATAGACAAAACGCGGCTATATTAAATGCCACATCT
GATGCTGTCATCACTATCAATCATAAATTGGTGATTGTTTCGGCAAACAAAGCAAGTTTTGATATGTTTCGATTTCATTC
CCCACTTGATTTAATCGGACAAAATGTTCGGCTCTTATTTACAGAAAACAAGATGCAAAAATACATGAATCAAGTTTCGA
GTGTATTGAGTCAGGAGCCAAATAAATCGACCTTACAACTTTCTGATGTGACATTACAAAGATCCGATTCAACTACTTTC
CTTGCTGATATTGCCATTTGTTCTTATAGTTTGGATGGAGATTCTTATTATACTGGTGTGATCCGCGATGTCACACAAAA
GAAATTGATGGTGGAACAACTCATCCATTCTGAAAGGAGAGCATTTTTATCAGTCGTAGCGGCAAGCATTGGTCACGAAA
TTAACAATTCACTTACGGCCATCCAAGGATTTGTGGAAATGGCTTCGCGTGAAAATGCTGACCTAATGCTGAAGGATCGT
GCCCTAAAAGTCACACTCAACCAAACTGAGAAACTCAGAGCATTAACTTCCAATTTGTTGCAACTTGGTAAGTCGGTAAA
ATCCAATCATGAAAAAACTGAAATTCTCAATTTGAATGGAGAAATCACTGCTGTATTACAAGTATTTAAAGAAACGGCAA
AATTAAAATACTGCCAAATCAAACGCGATGAAGCGAATGAAACCATTCCATTCCGCATGAACTCAGACCAATTTGCACTT
TTACTTTCTAATATTTTGTTAAACGCGGCCGATGCGACAAATAACATTGGAACTATTGAAATTAAGGCATACCTTCAGAA
TCATAAGGCTCACCTAATCATTACAGATGATGGTGAGGGGATGTCTCCTGAGACCTTAGAAAAAATTTATGAACCATATT
TCACAACAAAGGAATTAGGAAAAGGAACCGGTCTTGGAATGTTTGTCGTCAAACAAATAGTCGATAATTTCGAAATTGAT
CTGCAAATCGATTCTAATCCTGGAAAAGGATCTAAATTTCATTTTATTTTTCCTGAGGTTTCCGAATCCTAG

Upstream 100 bases:

>100_bases
AAAAAAATCATCGCCGAACATGGCGGGGAAATTTATATCAAAACAAAAAATGGAGTGGGAACAAAGGTAGAAATATTCCT
CCCTGCTCATACGGATGAAA

Downstream 100 bases:

>100_bases
TTGGTAACGTGAATTCTGGAATCAATGAATCTCAATTAGAATTTATCCATACTCGTTATGGTAAAACTTGTACAGTGACT
CCCTTACAAGAAGAAGCTTC

Product: putative sensor protein

Products: NA

Alternate protein names: Stage II sporulation protein F; Stage II sporulation protein J [H]

Number of amino acids: Translated: 503; Mature: 503

Protein sequence:

>503_residues
MDKILIIDDEEDIRIALKRVLSREGYQIELSESASDAVKRIETGETFSLVISDILMSGMSGIDFTKFIAEKNINLPVILI
TGNPNLSSAEAAIRYHAYEYISKPVDRTQLLSVVKRALEVKNQKDSDLEKLLLSEKLEKALRTQNLDLNRQNAAILNATS
DAVITINHKLVIVSANKASFDMFRFHSPLDLIGQNVRLLFTENKMQKYMNQVSSVLSQEPNKSTLQLSDVTLQRSDSTTF
LADIAICSYSLDGDSYYTGVIRDVTQKKLMVEQLIHSERRAFLSVVAASIGHEINNSLTAIQGFVEMASRENADLMLKDR
ALKVTLNQTEKLRALTSNLLQLGKSVKSNHEKTEILNLNGEITAVLQVFKETAKLKYCQIKRDEANETIPFRMNSDQFAL
LLSNILLNAADATNNIGTIEIKAYLQNHKAHLIITDDGEGMSPETLEKIYEPYFTTKELGKGTGLGMFVVKQIVDNFEID
LQIDSNPGKGSKFHFIFPEVSES

Sequences:

>Translated_503_residues
MDKILIIDDEEDIRIALKRVLSREGYQIELSESASDAVKRIETGETFSLVISDILMSGMSGIDFTKFIAEKNINLPVILI
TGNPNLSSAEAAIRYHAYEYISKPVDRTQLLSVVKRALEVKNQKDSDLEKLLLSEKLEKALRTQNLDLNRQNAAILNATS
DAVITINHKLVIVSANKASFDMFRFHSPLDLIGQNVRLLFTENKMQKYMNQVSSVLSQEPNKSTLQLSDVTLQRSDSTTF
LADIAICSYSLDGDSYYTGVIRDVTQKKLMVEQLIHSERRAFLSVVAASIGHEINNSLTAIQGFVEMASRENADLMLKDR
ALKVTLNQTEKLRALTSNLLQLGKSVKSNHEKTEILNLNGEITAVLQVFKETAKLKYCQIKRDEANETIPFRMNSDQFAL
LLSNILLNAADATNNIGTIEIKAYLQNHKAHLIITDDGEGMSPETLEKIYEPYFTTKELGKGTGLGMFVVKQIVDNFEID
LQIDSNPGKGSKFHFIFPEVSES
>Mature_503_residues
MDKILIIDDEEDIRIALKRVLSREGYQIELSESASDAVKRIETGETFSLVISDILMSGMSGIDFTKFIAEKNINLPVILI
TGNPNLSSAEAAIRYHAYEYISKPVDRTQLLSVVKRALEVKNQKDSDLEKLLLSEKLEKALRTQNLDLNRQNAAILNATS
DAVITINHKLVIVSANKASFDMFRFHSPLDLIGQNVRLLFTENKMQKYMNQVSSVLSQEPNKSTLQLSDVTLQRSDSTTF
LADIAICSYSLDGDSYYTGVIRDVTQKKLMVEQLIHSERRAFLSVVAASIGHEINNSLTAIQGFVEMASRENADLMLKDR
ALKVTLNQTEKLRALTSNLLQLGKSVKSNHEKTEILNLNGEITAVLQVFKETAKLKYCQIKRDEANETIPFRMNSDQFAL
LLSNILLNAADATNNIGTIEIKAYLQNHKAHLIITDDGEGMSPETLEKIYEPYFTTKELGKGTGLGMFVVKQIVDNFEID
LQIDSNPGKGSKFHFIFPEVSES

Specific function: Phosphorylates the sporulation-regulatory proteins spo0A and spo0F. It also autophosphorylates in the presence of ATP [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=239, Percent_Identity=32.2175732217573, Blast_Score=111, Evalue=9e-26,
Organism=Escherichia coli, GI1788549, Length=370, Percent_Identity=27.027027027027, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1786600, Length=248, Percent_Identity=29.4354838709677, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1788713, Length=276, Percent_Identity=23.9130434782609, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1790299, Length=115, Percent_Identity=30.4347826086957, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1790300, Length=378, Percent_Identity=23.2804232804233, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1788550, Length=124, Percent_Identity=29.8387096774194, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1788905, Length=165, Percent_Identity=29.6969696969697, Blast_Score=62, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR013655
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS; PF08447 PAS_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 56319; Mature: 56319

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKILIIDDEEDIRIALKRVLSREGYQIELSESASDAVKRIETGETFSLVISDILMSGMS
CCCEEEEECCCHHHHHHHHHHCCCCCEEEECCCHHHHHHHHCCCCHHHHHHHHHHHHCCC
GIDFTKFIAEKNINLPVILITGNPNLSSAEAAIRYHAYEYISKPVDRTQLLSVVKRALEV
CCHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
KNQKDSDLEKLLLSEKLEKALRTQNLDLNRQNAAILNATSDAVITINHKLVIVSANKASF
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCEEEEEEEEEEEEECCCCCC
DMFRFHSPLDLIGQNVRLLFTENKMQKYMNQVSSVLSQEPNKSTLQLSDVTLQRSDSTTF
HHHHHCCCHHHCCCCEEEEEECHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCEE
LADIAICSYSLDGDSYYTGVIRDVTQKKLMVEQLIHSERRAFLSVVAASIGHEINNSLTA
EEEEEEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
IQGFVEMASRENADLMLKDRALKVTLNQTEKLRALTSNLLQLGKSVKSNHEKTEILNLNG
HHHHHHHHCCCCCCEEEECCEEEEEECHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCC
EITAVLQVFKETAKLKYCQIKRDEANETIPFRMNSDQFALLLSNILLNAADATNNIGTIE
HHHHHHHHHHHHHCEEEEEEECCCCCCEECEEECCCHHHHHHHHHHHCCCCCCCCCEEEE
IKAYLQNHKAHLIITDDGEGMSPETLEKIYEPYFTTKELGKGTGLGMFVVKQIVDNFEID
EEEEECCCCEEEEEECCCCCCCHHHHHHHHCCCCCHHHHCCCCCHHHHHHHHHHCCCEEE
LQIDSNPGKGSKFHFIFPEVSES
EEECCCCCCCCEEEEECCCCCCC
>Mature Secondary Structure
MDKILIIDDEEDIRIALKRVLSREGYQIELSESASDAVKRIETGETFSLVISDILMSGMS
CCCEEEEECCCHHHHHHHHHHCCCCCEEEECCCHHHHHHHHCCCCHHHHHHHHHHHHCCC
GIDFTKFIAEKNINLPVILITGNPNLSSAEAAIRYHAYEYISKPVDRTQLLSVVKRALEV
CCHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
KNQKDSDLEKLLLSEKLEKALRTQNLDLNRQNAAILNATSDAVITINHKLVIVSANKASF
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCEEEEEEEEEEEEECCCCCC
DMFRFHSPLDLIGQNVRLLFTENKMQKYMNQVSSVLSQEPNKSTLQLSDVTLQRSDSTTF
HHHHHCCCHHHCCCCEEEEEECHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCEE
LADIAICSYSLDGDSYYTGVIRDVTQKKLMVEQLIHSERRAFLSVVAASIGHEINNSLTA
EEEEEEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
IQGFVEMASRENADLMLKDRALKVTLNQTEKLRALTSNLLQLGKSVKSNHEKTEILNLNG
HHHHHHHHCCCCCCEEEECCEEEEEECHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCC
EITAVLQVFKETAKLKYCQIKRDEANETIPFRMNSDQFALLLSNILLNAADATNNIGTIE
HHHHHHHHHHHHHCEEEEEEECCCCCCEECEEECCCHHHHHHHHHHHCCCCCCCCCEEEE
IKAYLQNHKAHLIITDDGEGMSPETLEKIYEPYFTTKELGKGTGLGMFVVKQIVDNFEID
EEEEECCCCEEEEEECCCCCCCHHHHHHHHCCCCCHHHHCCCCCHHHHHHHHHHCCCEEE
LQIDSNPGKGSKFHFIFPEVSES
EEECCCCCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2104615; 2509430; 9384377 [H]